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The map label for this gene is infB

Identifier: 88608686

GI number: 88608686

Start: 446040

End: 448379

Strand: Direct

Name: infB

Synonym: NSE_0522

Alternate gene names: 88608686

Gene position: 446040-448379 (Clockwise)

Preceding gene: 88608874

Following gene: 88608159

Centisome position: 51.93

GC content: 42.09

Gene sequence:

>2340_bases
ATGGATACTAAAAAAAGGCTCAGCTTGAATCTGAAGAACGGGAAAGAAGAATTGTCGCGCAGGGCCCTGAGAGAGTTTAT
CGGCAAGTCTTCCACTGGTGGCAGTACTCAGACTGCAGAGAGTGGTGGGGATCGTGCCAGTCATCTAACCAGACAAGAGG
AACGAAACAGGTTGGATGCACTTCATAGATATCGGAAAGCGAATATGTCGGTAGCTGCCCAGGAAAATTCTGTGCAAGAA
GAAGCGGTAGAAGAGTCTACGCAAGCTGCCCTTATTTTGCCAGCTTGGGCAAAGATATCTTCAACTCGCACTGGTAATTC
ATGTTTAACAAAAGCAGATGTGCAACCAAACATACAGGAAGATCCCCTATCACTTGTACAACTAGATAATCCAGAAAGGG
ATAGCTCTTCTTTGAAAAAGGGTAAAAAAGGACATAAGGCTTCACAGAACAAAGATGGATGGAAGAATCAAGAGATAAGA
AAGCGGAAAGATGCATTGGACCTTCAGGTAAAGCTGCAGAACATTGATGGAAAAGAATACTTTGAGAAGGCTCCCTTGAG
ATCGCCAAGTCTGAGAAGAAAGTCGTCTAGGGGTTCCGATATTTTACAGAGAAAAAATGTGAACCCAAAACGCGGACAGC
TCATCATGATAGATAAGCGCTCGGTAACACTGCGAGAGCTTTCTGTATTACTCTCTGAACCCATGGTGCGGATGAAGATG
ATCCTAAAAAAAATGGGAGAGATTTGCGGAGAAGATGATTATCTCAAGCAGGAGACTGTTGAACTATTGGCACTGGAACT
TGGCCACGAAATAAAGTACAAGCCCTCTACAGCTGAGATCTTTGCCGCCACAAGAAGGAACACAGATGAATCTGATCTAA
GGCCAAGAGTTCCTGTAGTCACTGTCATGGGACATATAGACCACGGGAAGACAACTTTGTTAGATGCGCTAAGGAATACA
AGTCTCACAGCAACTGAAAGTGGAGGCATAACACAACACATAGGGGCTTATCAGGTTCAGGTGGGTGATAGAAGTATTAC
TTTTATAGATACACCAGGGCACGCAGCCTTCACCTCAATGAGAATGAGAGGTGCAAAAGTTACAGATATAGTCGTTCTTG
TCGTTGCAGCAGATGATGGTGTGAACAAGCAGACTATCGAAGCAATTAAGCATGCAAAAGCAGCAGAGGTGCCAATCATT
GTTGCAATAAATAAGATCGACAAAGAATCTGCTAACCCTGCAAGAGTTTTCACTGAGCTTGCGCAGCACGGGGTGATAGT
CGAGGAAATGCATGGTGATGTTATGAGTGTTAACATCTCTGCAAAAAATCATATCAATCTGGATAAATTGAAAGAAGTGA
TCTTAATTCAAGCTGATTTTCTTGATTTGAAATATAATCCTAAACAGCCTGCAGAAGGTGTGATTATCGAATCTAGAGTT
GATAGAAAGTGCGGCGCTTTCTCTACTTTAATAGTAAAAGAAGGCGAACTGAAGACTGGACAGGTAGCTGTTCTTGGATC
CTTCTACGGAAAGATAAGAAGTATGATGACTTCGTCAGGGGAGATTGTCGGAAGTGCAGTCGCAGGAACTCCTGTTGAAG
TGATGGGTTTAAGTGAGGCTCCGGAACCAGGTACATCATTTTTCGTTGTCAATAGCGAAAAAGAAGCAAAAATGCTTGTC
AGCAGCCACGAGTATATAAAGGAGGATTCCTATAAAAGACCCTTGCGTGATTCATTCGAAAGCGATAAGCATAAGCTGAA
TTTCATAATCAAAGCCGACGTAAACGGTTCAGTGGAGGCACTTGTACAGTCAATAAGTGGGCTTTCTCATCCAGAAGTTG
ATATTGCTATCGTACACACTGGTATTGGTGATATAAGTGATTCTGATGTGTTGCTTGCACAAGTTTCTGGGGCCTACATT
GTAGGTTTCAGGGTTAAGACGGAAAAAACTGTAAAAGATTACCCAAAGATTATTTTTCAATCGATCATATATGACGTAAT
AAAGAAAGTTCAAGAAATGGTCGAACTTCTCGTCTCACCAAAGACACGAGAGGTTATTTTAGGTTCCGCAGTAGTCAAAG
AGGTCTTTACACTTTCAGATAAATCTAATGTCGCAGGATGCCAAGTAAAGTCTGGAGTGATCAAAAAATCTGCTTCTATA
AGGCTTTTGAGAAATGGAGAGAACATAAAGGATCTTTCAATAAAATCATTAAAGCGCTTCAGGGAAGAAGTAAAAGAGGT
GAAATCTGGTTACGAATGCGGGATCCTGCTTAATGGATATAACGAGATCAAGGTTGGTGACATCTTGGAGTCGTTTGGGC
TGCTTTCAAATGAGGAATAG

Upstream 100 bases:

>100_bases
ATTAAGAGAGGTCATTGGAGATAATGGATTTGGTGTGCCGTTGCTAGAACAGCTTATCATCAGATCAAGGAAGACTCTGG
GTTGGTTATAGTGCAGGCAT

Downstream 100 bases:

>100_bases
AAGCTTATATGAATCCGATAAAGAAGTTACGGGTTGAATCTTTGCTTAGAAGAAAAATAACTGAGATTATTAATAAAACC
TTTGGCCCGGTGGCTGCAGT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MDTKKRLSLNLKNGKEELSRRALREFIGKSSTGGSTQTAESGGDRASHLTRQEERNRLDALHRYRKANMSVAAQENSVQE
EAVEESTQAALILPAWAKISSTRTGNSCLTKADVQPNIQEDPLSLVQLDNPERDSSSLKKGKKGHKASQNKDGWKNQEIR
KRKDALDLQVKLQNIDGKEYFEKAPLRSPSLRRKSSRGSDILQRKNVNPKRGQLIMIDKRSVTLRELSVLLSEPMVRMKM
ILKKMGEICGEDDYLKQETVELLALELGHEIKYKPSTAEIFAATRRNTDESDLRPRVPVVTVMGHIDHGKTTLLDALRNT
SLTATESGGITQHIGAYQVQVGDRSITFIDTPGHAAFTSMRMRGAKVTDIVVLVVAADDGVNKQTIEAIKHAKAAEVPII
VAINKIDKESANPARVFTELAQHGVIVEEMHGDVMSVNISAKNHINLDKLKEVILIQADFLDLKYNPKQPAEGVIIESRV
DRKCGAFSTLIVKEGELKTGQVAVLGSFYGKIRSMMTSSGEIVGSAVAGTPVEVMGLSEAPEPGTSFFVVNSEKEAKMLV
SSHEYIKEDSYKRPLRDSFESDKHKLNFIIKADVNGSVEALVQSISGLSHPEVDIAIVHTGIGDISDSDVLLAQVSGAYI
VGFRVKTEKTVKDYPKIIFQSIIYDVIKKVQEMVELLVSPKTREVILGSAVVKEVFTLSDKSNVAGCQVKSGVIKKSASI
RLLRNGENIKDLSIKSLKRFREEVKEVKSGYECGILLNGYNEIKVGDILESFGLLSNEE

Sequences:

>Translated_779_residues
MDTKKRLSLNLKNGKEELSRRALREFIGKSSTGGSTQTAESGGDRASHLTRQEERNRLDALHRYRKANMSVAAQENSVQE
EAVEESTQAALILPAWAKISSTRTGNSCLTKADVQPNIQEDPLSLVQLDNPERDSSSLKKGKKGHKASQNKDGWKNQEIR
KRKDALDLQVKLQNIDGKEYFEKAPLRSPSLRRKSSRGSDILQRKNVNPKRGQLIMIDKRSVTLRELSVLLSEPMVRMKM
ILKKMGEICGEDDYLKQETVELLALELGHEIKYKPSTAEIFAATRRNTDESDLRPRVPVVTVMGHIDHGKTTLLDALRNT
SLTATESGGITQHIGAYQVQVGDRSITFIDTPGHAAFTSMRMRGAKVTDIVVLVVAADDGVNKQTIEAIKHAKAAEVPII
VAINKIDKESANPARVFTELAQHGVIVEEMHGDVMSVNISAKNHINLDKLKEVILIQADFLDLKYNPKQPAEGVIIESRV
DRKCGAFSTLIVKEGELKTGQVAVLGSFYGKIRSMMTSSGEIVGSAVAGTPVEVMGLSEAPEPGTSFFVVNSEKEAKMLV
SSHEYIKEDSYKRPLRDSFESDKHKLNFIIKADVNGSVEALVQSISGLSHPEVDIAIVHTGIGDISDSDVLLAQVSGAYI
VGFRVKTEKTVKDYPKIIFQSIIYDVIKKVQEMVELLVSPKTREVILGSAVVKEVFTLSDKSNVAGCQVKSGVIKKSASI
RLLRNGENIKDLSIKSLKRFREEVKEVKSGYECGILLNGYNEIKVGDILESFGLLSNEE
>Mature_779_residues
MDTKKRLSLNLKNGKEELSRRALREFIGKSSTGGSTQTAESGGDRASHLTRQEERNRLDALHRYRKANMSVAAQENSVQE
EAVEESTQAALILPAWAKISSTRTGNSCLTKADVQPNIQEDPLSLVQLDNPERDSSSLKKGKKGHKASQNKDGWKNQEIR
KRKDALDLQVKLQNIDGKEYFEKAPLRSPSLRRKSSRGSDILQRKNVNPKRGQLIMIDKRSVTLRELSVLLSEPMVRMKM
ILKKMGEICGEDDYLKQETVELLALELGHEIKYKPSTAEIFAATRRNTDESDLRPRVPVVTVMGHIDHGKTTLLDALRNT
SLTATESGGITQHIGAYQVQVGDRSITFIDTPGHAAFTSMRMRGAKVTDIVVLVVAADDGVNKQTIEAIKHAKAAEVPII
VAINKIDKESANPARVFTELAQHGVIVEEMHGDVMSVNISAKNHINLDKLKEVILIQADFLDLKYNPKQPAEGVIIESRV
DRKCGAFSTLIVKEGELKTGQVAVLGSFYGKIRSMMTSSGEIVGSAVAGTPVEVMGLSEAPEPGTSFFVVNSEKEAKMLV
SSHEYIKEDSYKRPLRDSFESDKHKLNFIIKADVNGSVEALVQSISGLSHPEVDIAIVHTGIGDISDSDVLLAQVSGAYI
VGFRVKTEKTVKDYPKIIFQSIIYDVIKKVQEMVELLVSPKTREVILGSAVVKEVFTLSDKSNVAGCQVKSGVIKKSASI
RLLRNGENIKDLSIKSLKRFREEVKEVKSGYECGILLNGYNEIKVGDILESFGLLSNEE

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=544, Percent_Identity=39.3382352941176, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI53729337, Length=544, Percent_Identity=39.3382352941176, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI84043963, Length=131, Percent_Identity=42.7480916030534, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI157426893, Length=230, Percent_Identity=27.3913043478261, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI18390331, Length=142, Percent_Identity=33.0985915492958, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1789559, Length=573, Percent_Identity=41.5357766143106, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI2367247, Length=115, Percent_Identity=39.1304347826087, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI48994988, Length=324, Percent_Identity=27.1604938271605, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788922, Length=222, Percent_Identity=27.4774774774775, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=132, Percent_Identity=37.1212121212121, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1789737, Length=132, Percent_Identity=37.1212121212121, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=536, Percent_Identity=34.8880597014925, Blast_Score=291, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI212656558, Length=597, Percent_Identity=24.7906197654941, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=219, Percent_Identity=32.4200913242009, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=563, Percent_Identity=34.8134991119005, Blast_Score=303, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6319282, Length=131, Percent_Identity=39.6946564885496, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI28572034, Length=526, Percent_Identity=39.5437262357414, Blast_Score=341, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24656849, Length=145, Percent_Identity=41.3793103448276, Blast_Score=107, Evalue=4e-23,
Organism=Drosophila melanogaster, GI160714833, Length=74, Percent_Identity=67.5675675675676, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI78706572, Length=279, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582462, Length=146, Percent_Identity=31.5068493150685, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922690, Length=129, Percent_Identity=34.8837209302326, Blast_Score=68, Evalue=3e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013575
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF08364 IF2_assoc; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 85938; Mature: 85938

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKKRLSLNLKNGKEELSRRALREFIGKSSTGGSTQTAESGGDRASHLTRQEERNRLDA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LHRYRKANMSVAAQENSVQEEAVEESTQAALILPAWAKISSTRTGNSCLTKADVQPNIQE
HHHHHHCCCEEEECCCCHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCEEECCCCCCCCC
DPLSLVQLDNPERDSSSLKKGKKGHKASQNKDGWKNQEIRKRKDALDLQVKLQNIDGKEY
CCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCHHH
FEKAPLRSPSLRRKSSRGSDILQRKNVNPKRGQLIMIDKRSVTLRELSVLLSEPMVRMKM
HHHCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHH
ILKKMGEICGEDDYLKQETVELLALELGHEIKYKPSTAEIFAATRRNTDESDLRPRVPVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCHHCCCCCCCEE
TVMGHIDHGKTTLLDALRNTSLTATESGGITQHIGAYQVQVGDRSITFIDTPGHAAFTSM
EEEECCCCCHHHHHHHHHCCCCEEECCCCCHHCCCEEEEEECCCEEEEEECCCCHHHHHH
RMRGAKVTDIVVLVVAADDGVNKQTIEAIKHAKAAEVPIIVAINKIDKESANPARVFTEL
HHCCCEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHH
AQHGVIVEEMHGDVMSVNISAKNHINLDKLKEVILIQADFLDLKYNPKQPAEGVIIESRV
HHCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHEEEEEEEEEECCCCCCCCCEEEECCC
DRKCGAFSTLIVKEGELKTGQVAVLGSFYGKIRSMMTSSGEIVGSAVAGTPVEVMGLSEA
CCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCEEEHHCCCCCEEEEECCCC
PEPGTSFFVVNSEKEAKMLVSSHEYIKEDSYKRPLRDSFESDKHKLNFIIKADVNGSVEA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEEEEECCCCCHHH
LVQSISGLSHPEVDIAIVHTGIGDISDSDVLLAQVSGAYIVGFRVKTEKTVKDYPKIIFQ
HHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCEEEEEEEEECCHHHHHHHHHHHH
SIIYDVIKKVQEMVELLVSPKTREVILGSAVVKEVFTLSDKSNVAGCQVKSGVIKKSASI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHCCCCE
RLLRNGENIKDLSIKSLKRFREEVKEVKSGYECGILLNGYNEIKVGDILESFGLLSNEE
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTKKRLSLNLKNGKEELSRRALREFIGKSSTGGSTQTAESGGDRASHLTRQEERNRLDA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LHRYRKANMSVAAQENSVQEEAVEESTQAALILPAWAKISSTRTGNSCLTKADVQPNIQE
HHHHHHCCCEEEECCCCHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCEEECCCCCCCCC
DPLSLVQLDNPERDSSSLKKGKKGHKASQNKDGWKNQEIRKRKDALDLQVKLQNIDGKEY
CCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECCCCHHH
FEKAPLRSPSLRRKSSRGSDILQRKNVNPKRGQLIMIDKRSVTLRELSVLLSEPMVRMKM
HHHCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHH
ILKKMGEICGEDDYLKQETVELLALELGHEIKYKPSTAEIFAATRRNTDESDLRPRVPVV
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCHHCCCCCCCEE
TVMGHIDHGKTTLLDALRNTSLTATESGGITQHIGAYQVQVGDRSITFIDTPGHAAFTSM
EEEECCCCCHHHHHHHHHCCCCEEECCCCCHHCCCEEEEEECCCEEEEEECCCCHHHHHH
RMRGAKVTDIVVLVVAADDGVNKQTIEAIKHAKAAEVPIIVAINKIDKESANPARVFTEL
HHCCCEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHH
AQHGVIVEEMHGDVMSVNISAKNHINLDKLKEVILIQADFLDLKYNPKQPAEGVIIESRV
HHCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHEEEEEEEEEECCCCCCCCCEEEECCC
DRKCGAFSTLIVKEGELKTGQVAVLGSFYGKIRSMMTSSGEIVGSAVAGTPVEVMGLSEA
CCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHCCCCEEEHHCCCCCEEEEECCCC
PEPGTSFFVVNSEKEAKMLVSSHEYIKEDSYKRPLRDSFESDKHKLNFIIKADVNGSVEA
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEEEEEECCCCCHHH
LVQSISGLSHPEVDIAIVHTGIGDISDSDVLLAQVSGAYIVGFRVKTEKTVKDYPKIIFQ
HHHHHHCCCCCCEEEEEEEECCCCCCCCCEEEEEECCEEEEEEEEECCHHHHHHHHHHHH
SIIYDVIKKVQEMVELLVSPKTREVILGSAVVKEVFTLSDKSNVAGCQVKSGVIKKSASI
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHCCCCE
RLLRNGENIKDLSIKSLKRFREEVKEVKSGYECGILLNGYNEIKVGDILESFGLLSNEE
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA