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The map label for this gene is bioD

Identifier: 88608873

GI number: 88608873

Start: 539613

End: 540362

Strand: Direct

Name: bioD

Synonym: NSE_0617

Alternate gene names: 88608873

Gene position: 539613-540362 (Clockwise)

Preceding gene: 88608178

Following gene: 88608024

Centisome position: 62.82

GC content: 42.53

Gene sequence:

>750_bases
ATGGCCATCTTTATCACTGGAACAGACACAAATGTCGGCAAAACAATAATAAGCACTTGGATATGCTTGCACTTGGGTTG
GGGTTATTTTAAGCCAATACAAACCGGAGGTAATAGTGACAGCGATTTCGTTTCCAGTACCACTGGTGTGCCAGCCTATG
ATTCGGTCTTTTCCTTCCCTGATCCAATTGCACCACATGTTGCAGCTAAGATGAGTGGAAGCTCAATTGATATTCTCAAA
ATTCAACTACCTGAATATAGGAAAAGCCTTAGCAACAAAATAAACTCTATTCCGACCACAGAACACGGGCGAAGTAAGTT
CATCAGCGCAACTACTCAAACAAAGTATTGGGAAAAAGAAAATAACAAAATTGTTATCGAGGGTGCTGGCGGTGTCCTTG
TGCCCCTCCAGGAAAACGGGACTAAAATGGTGGATCTGATTCAACACCTGAACGTACCGGTGATAATTGTATCGCGCTCT
ACTCTAGGCGCAATTAACCACACTTTACTTACACTCGAAGCACTACAGGCAAGAGAAATAAAAGTTCTCGGCATAGTGAT
AAACTCAATGTGCGAGGACTTTCTGGACTACAATTCAAAAGCAATAGCAGAATACGGTTCGACCGAGATCTTAGCCACTT
TTCCATACCTAAAGGAAGTAACCAGAGATAGCATCCTCAGCGTACAGATGGGTGATTCAATGAAAGCACTACTGGAATCC
ACACTAAAATGCAAAAATTCGCTGCTCTAG

Upstream 100 bases:

>100_bases
TCTAAAACAGCCTTAGCGAATTACAAGCAAGCACATCTTGAAGTACTAACGCAGTGTGATTTATATAAACAAGCAACCCT
CCGTATTGGCTGAGTAGAAC

Downstream 100 bases:

>100_bases
TGATTCTTGGAGCAAGATATTTCTGTATACGTCATACAAAATTGAGCTATGGAGTTATTATTTCGAGCTGACAAATTTCA
ACTTCTTTGAAAAACAGAAA

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MAIFITGTDTNVGKTIISTWICLHLGWGYFKPIQTGGNSDSDFVSSTTGVPAYDSVFSFPDPIAPHVAAKMSGSSIDILK
IQLPEYRKSLSNKINSIPTTEHGRSKFISATTQTKYWEKENNKIVIEGAGGVLVPLQENGTKMVDLIQHLNVPVIIVSRS
TLGAINHTLLTLEALQAREIKVLGIVINSMCEDFLDYNSKAIAEYGSTEILATFPYLKEVTRDSILSVQMGDSMKALLES
TLKCKNSLL

Sequences:

>Translated_249_residues
MAIFITGTDTNVGKTIISTWICLHLGWGYFKPIQTGGNSDSDFVSSTTGVPAYDSVFSFPDPIAPHVAAKMSGSSIDILK
IQLPEYRKSLSNKINSIPTTEHGRSKFISATTQTKYWEKENNKIVIEGAGGVLVPLQENGTKMVDLIQHLNVPVIIVSRS
TLGAINHTLLTLEALQAREIKVLGIVINSMCEDFLDYNSKAIAEYGSTEILATFPYLKEVTRDSILSVQMGDSMKALLES
TLKCKNSLL
>Mature_248_residues
AIFITGTDTNVGKTIISTWICLHLGWGYFKPIQTGGNSDSDFVSSTTGVPAYDSVFSFPDPIAPHVAAKMSGSSIDILKI
QLPEYRKSLSNKINSIPTTEHGRSKFISATTQTKYWEKENNKIVIEGAGGVLVPLQENGTKMVDLIQHLNVPVIIVSRST
LGAINHTLLTLEALQAREIKVLGIVINSMCEDFLDYNSKAIAEYGSTEILATFPYLKEVTRDSILSVQMGDSMKALLEST
LKCKNSLL

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=242, Percent_Identity=27.6859504132231, Blast_Score=67, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324385, Length=221, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_NEOSM (Q2GDE9)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506497.1
- ProteinModelPortal:   Q2GDE9
- SMR:   Q2GDE9
- STRING:   Q2GDE9
- GeneID:   3931611
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0617
- TIGR:   NSE_0617
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   VEDFEID
- PhylomeDB:   Q2GDE9
- ProtClustDB:   CLSK2528200
- BioCyc:   NSEN222891:NSE_0617-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: NA

EC number: =6.3.3.3

Molecular weight: Translated: 27217; Mature: 27085

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIFITGTDTNVGKTIISTWICLHLGWGYFKPIQTGGNSDSDFVSSTTGVPAYDSVFSFP
CEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCC
DPIAPHVAAKMSGSSIDILKIQLPEYRKSLSNKINSIPTTEHGRSKFISATTQTKYWEKE
CCCCCHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCHHEEECCCCCCEEECC
NNKIVIEGAGGVLVPLQENGTKMVDLIQHLNVPVIIVSRSTLGAINHTLLTLEALQAREI
CCEEEEEECCCEEEEECCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCC
KVLGIVINSMCEDFLDYNSKAIAEYGSTEILATFPYLKEVTRDSILSVQMGDSMKALLES
HHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHHHEEEEECCCHHHHHHHH
TLKCKNSLL
HHHHHHCCC
>Mature Secondary Structure 
AIFITGTDTNVGKTIISTWICLHLGWGYFKPIQTGGNSDSDFVSSTTGVPAYDSVFSFP
EEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCC
DPIAPHVAAKMSGSSIDILKIQLPEYRKSLSNKINSIPTTEHGRSKFISATTQTKYWEKE
CCCCCHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCHHEEECCCCCCEEECC
NNKIVIEGAGGVLVPLQENGTKMVDLIQHLNVPVIIVSRSTLGAINHTLLTLEALQAREI
CCEEEEEECCCEEEEECCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCC
KVLGIVINSMCEDFLDYNSKAIAEYGSTEILATFPYLKEVTRDSILSVQMGDSMKALLES
HHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHHHEEEEECCCHHHHHHHH
TLKCKNSLL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA