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The map label for this gene is engD [H]

Identifier: 88608807

GI number: 88608807

Start: 808080

End: 809132

Strand: Direct

Name: engD [H]

Synonym: NSE_0910

Alternate gene names: 88608807

Gene position: 808080-809132 (Clockwise)

Preceding gene: 88608049

Following gene: 88608105

Centisome position: 94.07

GC content: 40.27

Gene sequence:

>1053_bases
ATGGGCTTAAAGTGCGGAATCGTCGGGCTTCCAAATGTTGGAAAGTCGACATTGTTTAATGCGATGACGCAAACACAAGC
AGCTGAAGCTGCAAACTATCCTTTTTGCACAATAGAACCAAACATTGCCAAGGTACCAGAATATGACGAACGTCTTGTTC
AAATAGCCAAGATTTCATCGGCACAAAAAATTATATTCAGCCAATTGGAAATCGTTGATATCGCAGGGCTAGTTAAGGGA
GCAAATAAAGGTGAGGGATTAGGTAATAAATTCCTTTCCCACATAAGAGAAGTAGATCTCATAATACACGTGCTGAGATG
TTTTCCAGATAACAATATAAGACACGTTCATGGAAAGGTAGACCCTGTGTACGATGCAGAAATCATAAAGACTGAGCTGC
TCCTCGCGGACATTCAGAGTCTGGAAAATCGTCTCAAAAAAAGTAAAGCCTCTAGTCAGATAATAGAACAGGCTCTAGAG
ATATTAAATAAAAAAAATCCTCTTCCATATGATCTAATGGATCAAGCATTTAAAGAACTGCATCTATTAAGTGCGAAGCC
GGTTCTATATGTGTGTAATGTCCCTCAAGAAGATATCGTCTCAGGAAACAATTTCAGCAAACAAGCAGAAAAATTCGCAC
AGGACAGCATGTCTGAAGCAGTTAAAGTTTCTGCAGCAATAGAAGCGGAAATCTCACTGTTAGAATCAGCAGAAGACCGC
AAACTTTTTTTGAATGAACTTGGCTTAGAAGAATCACAATTGAATAAGGTAATCCGGGCAAGTTCAAGGCTCCTCAACCT
CATTGTCTTTTTCACTTCAGGAGAAAAAGAAGCAAGAGCCTGGCCTATAAAAAGAGGTTCTACAGCGCCTCAGGCAGCTG
GAACGATTCATACAGACTTCGAAAAAAAGTTTATTAAGGCTGAAACAATAAGCTTCACAGACTATATCACCTTCGGCGGT
GAGCAAAAATGTAAAGCACTCGGCAAAATTCGTTCAGAGGGTCGCGAGTACGTCGTACAAGATGGAGATATAATCCTTTT
TAAACACGGGTAG

Upstream 100 bases:

>100_bases
AGGTCTTTCCAAGGTAAAGCAATTCACTACGCTTTCCATTGTAAAAGTTGCGTAAAAACAACAAAAAGTGTAGACTCCCA
CCAGGTAGTGAATTCTAGAT

Downstream 100 bases:

>100_bases
TACCATGCCAAAGATCAGCAAGGACATCCTAGCAAAGAGACTGAGAGAAAACATACAAAGACGGAAAAAACGCAAAAACA
GTGCACTATCTAAAGACAGC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MGLKCGIVGLPNVGKSTLFNAMTQTQAAEAANYPFCTIEPNIAKVPEYDERLVQIAKISSAQKIIFSQLEIVDIAGLVKG
ANKGEGLGNKFLSHIREVDLIIHVLRCFPDNNIRHVHGKVDPVYDAEIIKTELLLADIQSLENRLKKSKASSQIIEQALE
ILNKKNPLPYDLMDQAFKELHLLSAKPVLYVCNVPQEDIVSGNNFSKQAEKFAQDSMSEAVKVSAAIEAEISLLESAEDR
KLFLNELGLEESQLNKVIRASSRLLNLIVFFTSGEKEARAWPIKRGSTAPQAAGTIHTDFEKKFIKAETISFTDYITFGG
EQKCKALGKIRSEGREYVVQDGDIILFKHG

Sequences:

>Translated_350_residues
MGLKCGIVGLPNVGKSTLFNAMTQTQAAEAANYPFCTIEPNIAKVPEYDERLVQIAKISSAQKIIFSQLEIVDIAGLVKG
ANKGEGLGNKFLSHIREVDLIIHVLRCFPDNNIRHVHGKVDPVYDAEIIKTELLLADIQSLENRLKKSKASSQIIEQALE
ILNKKNPLPYDLMDQAFKELHLLSAKPVLYVCNVPQEDIVSGNNFSKQAEKFAQDSMSEAVKVSAAIEAEISLLESAEDR
KLFLNELGLEESQLNKVIRASSRLLNLIVFFTSGEKEARAWPIKRGSTAPQAAGTIHTDFEKKFIKAETISFTDYITFGG
EQKCKALGKIRSEGREYVVQDGDIILFKHG
>Mature_349_residues
GLKCGIVGLPNVGKSTLFNAMTQTQAAEAANYPFCTIEPNIAKVPEYDERLVQIAKISSAQKIIFSQLEIVDIAGLVKGA
NKGEGLGNKFLSHIREVDLIIHVLRCFPDNNIRHVHGKVDPVYDAEIIKTELLLADIQSLENRLKKSKASSQIIEQALEI
LNKKNPLPYDLMDQAFKELHLLSAKPVLYVCNVPQEDIVSGNNFSKQAEKFAQDSMSEAVKVSAAIEAEISLLESAEDRK
LFLNELGLEESQLNKVIRASSRLLNLIVFFTSGEKEARAWPIKRGSTAPQAAGTIHTDFEKKFIKAETISFTDYITFGGE
QKCKALGKIRSEGREYVVQDGDIILFKHG

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=43.9024390243902, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI58761502, Length=198, Percent_Identity=39.8989898989899, Blast_Score=134, Evalue=8e-32,
Organism=Escherichia coli, GI1787454, Length=362, Percent_Identity=51.9337016574586, Blast_Score=351, Evalue=3e-98,
Organism=Escherichia coli, GI1789574, Length=128, Percent_Identity=32.03125, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17509631, Length=364, Percent_Identity=41.2087912087912, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17552324, Length=102, Percent_Identity=32.3529411764706, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319499, Length=379, Percent_Identity=39.3139841688654, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=35.0923482849604, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321649, Length=159, Percent_Identity=36.4779874213836, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=41.8032786885246, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=41.8032786885246, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=41.8032786885246, Blast_Score=258, Evalue=6e-69,
Organism=Drosophila melanogaster, GI24640879, Length=325, Percent_Identity=38.7692307692308, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24585318, Length=185, Percent_Identity=31.8918918918919, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129375, Length=100, Percent_Identity=37, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 38788; Mature: 38657

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKCGIVGLPNVGKSTLFNAMTQTQAAEAANYPFCTIEPNIAKVPEYDERLVQIAKISS
CCCEECEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHH
AQKIIFSQLEIVDIAGLVKGANKGEGLGNKFLSHIREVDLIIHVLRCFPDNNIRHVHGKV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
DPVYDAEIIKTELLLADIQSLENRLKKSKASSQIIEQALEILNKKNPLPYDLMDQAFKEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HLLSAKPVLYVCNVPQEDIVSGNNFSKQAEKFAQDSMSEAVKVSAAIEAEISLLESAEDR
HHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KLFLNELGLEESQLNKVIRASSRLLNLIVFFTSGEKEARAWPIKRGSTAPQAAGTIHTDF
HHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
EKKFIKAETISFTDYITFGGEQKCKALGKIRSEGREYVVQDGDIILFKHG
HHHHHHHHEEEEEHEEECCCHHHHHHHHHHHHCCCEEEEECCCEEEEECC
>Mature Secondary Structure 
GLKCGIVGLPNVGKSTLFNAMTQTQAAEAANYPFCTIEPNIAKVPEYDERLVQIAKISS
CCEECEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHH
AQKIIFSQLEIVDIAGLVKGANKGEGLGNKFLSHIREVDLIIHVLRCFPDNNIRHVHGKV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
DPVYDAEIIKTELLLADIQSLENRLKKSKASSQIIEQALEILNKKNPLPYDLMDQAFKEL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
HLLSAKPVLYVCNVPQEDIVSGNNFSKQAEKFAQDSMSEAVKVSAAIEAEISLLESAEDR
HHHCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KLFLNELGLEESQLNKVIRASSRLLNLIVFFTSGEKEARAWPIKRGSTAPQAAGTIHTDF
HHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
EKKFIKAETISFTDYITFGGEQKCKALGKIRSEGREYVVQDGDIILFKHG
HHHHHHHHEEEEEHEEECCCHHHHHHHHHHHHCCCEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]