Click here to switch to the map view.

The map label for this gene is mdh

Identifier: 88608796

GI number: 88608796

Start: 840464

End: 841411

Strand: Reverse

Name: mdh

Synonym: NSE_0956

Alternate gene names: 88608796

Gene position: 841411-840464 (Counterclockwise)

Preceding gene: 88608771

Following gene: 88608702

Centisome position: 97.95

GC content: 43.99

Gene sequence:

>948_bases
ATGAAAGTAAGTTTAATTGGTGCTGGCAATATTGGTGGGACCTTGGCGTATTTAATAGCCTCGAAGAAGTTGGCTTCAGA
GGTCGAATTAATTGACGTTAACGGAGATCTTGCAAGAGGCAAAGCATTAGATGTTTCACAAACTCTTCCACTCATTGGTT
ATACAATGAAGATCAATGGTAGCGCCAATATGGAGAGAATCAAAGGCTCTTCGGTAATTATCATAACTGCCGGTATTCCA
CGAAAGCCTGGCATGACACGTGAGGAACTCATTGATGTGAATGCTGTTGTTATGAAAGAAGTAGGGGAAAAAATAAAAAA
GTTTGCCCCGAAAGCGTTTGTGATAGTTGTGACTAACCCGCTAGATGTAATGGTTTGGGTCCTTTACAAGGCAGCCGAAA
TCAGCCCTGACAAGATTGTTGGTATGGCAGGTGTACTCGATGCAAGCAGAATGAATCTTTTTCTTGCACAAGAGCTAGGT
GTTTCAGTAGCGGATGTTAAATCGCTGGTATTGGGTAGTCACGGTGACTCTATGGTTCCCTTGTTTAGGCACTCTACGGT
TTCTGGTATGAGCCTACCTGAGTTGGTTAGTGTGGGTTTAATAACGAAGGATAAGGTGGACTCAATTATTGAACGTACTC
GTAGTGGTGGTGCCGAGATAGTTGCCCTCTTGAAAACTGGTTCAGCCTACTACACTCCTGCTGCGTCTGTGCTGGAAATG
GCTGAGGCATATCTGTTGGATCAGAAAAAAACGCTCGTGTGCTCCGTGATGATGAGGGGTCGGTATGGAGTGGAGGATGA
TATCTTTTCTGGTATACCAGTTATCATGGGATCAGGTGGTGTGGAGCGTGTAATTGAACTTGATCTGACTCCTGATGAGA
GGAGGATGTTTGAAAATTCTGTTGCTGCAACGAGAAAATTAGTGCTGGAAGCGCGAAAATACTTTTAG

Upstream 100 bases:

>100_bases
ACAATGGGAATGCTACTAAACTAGAGCCTGTCATGGAAACTATGTATATTAATGAACTTCTGTTCTAGAATTGGCAGTTG
GGTGATAGGAGTGGGTTCGA

Downstream 100 bases:

>100_bases
AAAGCTGTCTGTTTTTATTGACGGCAAATTAGTGTGTGTAAGGGTTCCTGCATGCGTTTTGTGGTATTTGAAAAGAATTT
TCCTGTTTGATGAGCTATGT

Product: malate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKVSLIGAGNIGGTLAYLIASKKLASEVELIDVNGDLARGKALDVSQTLPLIGYTMKINGSANMERIKGSSVIIITAGIP
RKPGMTREELIDVNAVVMKEVGEKIKKFAPKAFVIVVTNPLDVMVWVLYKAAEISPDKIVGMAGVLDASRMNLFLAQELG
VSVADVKSLVLGSHGDSMVPLFRHSTVSGMSLPELVSVGLITKDKVDSIIERTRSGGAEIVALLKTGSAYYTPAASVLEM
AEAYLLDQKKTLVCSVMMRGRYGVEDDIFSGIPVIMGSGGVERVIELDLTPDERRMFENSVAATRKLVLEARKYF

Sequences:

>Translated_315_residues
MKVSLIGAGNIGGTLAYLIASKKLASEVELIDVNGDLARGKALDVSQTLPLIGYTMKINGSANMERIKGSSVIIITAGIP
RKPGMTREELIDVNAVVMKEVGEKIKKFAPKAFVIVVTNPLDVMVWVLYKAAEISPDKIVGMAGVLDASRMNLFLAQELG
VSVADVKSLVLGSHGDSMVPLFRHSTVSGMSLPELVSVGLITKDKVDSIIERTRSGGAEIVALLKTGSAYYTPAASVLEM
AEAYLLDQKKTLVCSVMMRGRYGVEDDIFSGIPVIMGSGGVERVIELDLTPDERRMFENSVAATRKLVLEARKYF
>Mature_315_residues
MKVSLIGAGNIGGTLAYLIASKKLASEVELIDVNGDLARGKALDVSQTLPLIGYTMKINGSANMERIKGSSVIIITAGIP
RKPGMTREELIDVNAVVMKEVGEKIKKFAPKAFVIVVTNPLDVMVWVLYKAAEISPDKIVGMAGVLDASRMNLFLAQELG
VSVADVKSLVLGSHGDSMVPLFRHSTVSGMSLPELVSVGLITKDKVDSIIERTRSGGAEIVALLKTGSAYYTPAASVLEM
AEAYLLDQKKTLVCSVMMRGRYGVEDDIFSGIPVIMGSGGVERVIELDLTPDERRMFENSVAATRKLVLEARKYF

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=301, Percent_Identity=28.9036544850498, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI221136809, Length=301, Percent_Identity=28.9036544850498, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI15082234, Length=301, Percent_Identity=28.5714285714286, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI9257228, Length=301, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4504973, Length=301, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI5031857, Length=301, Percent_Identity=28.9036544850498, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI260099723, Length=301, Percent_Identity=28.9036544850498, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI291575128, Length=302, Percent_Identity=28.8079470198675, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4557032, Length=302, Percent_Identity=28.8079470198675, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260099725, Length=225, Percent_Identity=30.6666666666667, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=31.4432989690722, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI21735621, Length=298, Percent_Identity=29.8657718120805, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI207028494, Length=187, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI103472011, Length=292, Percent_Identity=20.2054794520548, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI5174539, Length=219, Percent_Identity=27.3972602739726, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789632, Length=295, Percent_Identity=31.5254237288136, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=26.3698630136986, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17554310, Length=316, Percent_Identity=28.1645569620253, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=301, Percent_Identity=28.2392026578073, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324446, Length=275, Percent_Identity=28.7272727272727, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320125, Length=353, Percent_Identity=25.7790368271955, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17136226, Length=301, Percent_Identity=26.9102990033223, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24647881, Length=317, Percent_Identity=29.9684542586751, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI45550422, Length=317, Percent_Identity=25.8675078864353, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=27.9220779220779, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24663595, Length=291, Percent_Identity=25.7731958762887, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_NEOSM (Q2GCH6)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506820.1
- ProteinModelPortal:   Q2GCH6
- SMR:   Q2GCH6
- STRING:   Q2GCH6
- GeneID:   3931592
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0956
- TIGR:   NSE_0956
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   LDKPAND
- PhylomeDB:   Q2GCH6
- ProtClustDB:   CLSK2528156
- BioCyc:   NSEN222891:NSE_0956-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33852; Mature: 33852

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSLIGAGNIGGTLAYLIASKKLASEVELIDVNGDLARGKALDVSQTLPLIGYTMKING
CEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHCCCEEEEEEEECC
SANMERIKGSSVIIITAGIPRKPGMTREELIDVNAVVMKEVGEKIKKFAPKAFVIVVTNP
CCCHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECH
LDVMVWVLYKAAEISPDKIVGMAGVLDASRMNLFLAQELGVSVADVKSLVLGSHGDSMVP
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHEEEHHHHCCCHHHHHHHHHCCCCCCCCC
LFRHSTVSGMSLPELVSVGLITKDKVDSIIERTRSGGAEIVALLKTGSAYYTPAASVLEM
HHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHHHH
AEAYLLDQKKTLVCSVMMRGRYGVEDDIFSGIPVIMGSGGVERVIELDLTPDERRMFENS
HHHHHHCCCHHHHHHHHHCCCCCCCHHHHCCCCEEECCCCCEEEEEECCCCHHHHHHHHH
VAATRKLVLEARKYF
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVSLIGAGNIGGTLAYLIASKKLASEVELIDVNGDLARGKALDVSQTLPLIGYTMKING
CEEEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHCCCEEEEEEEECC
SANMERIKGSSVIIITAGIPRKPGMTREELIDVNAVVMKEVGEKIKKFAPKAFVIVVTNP
CCCHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECH
LDVMVWVLYKAAEISPDKIVGMAGVLDASRMNLFLAQELGVSVADVKSLVLGSHGDSMVP
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHEEEHHHHCCCHHHHHHHHHCCCCCCCCC
LFRHSTVSGMSLPELVSVGLITKDKVDSIIERTRSGGAEIVALLKTGSAYYTPAASVLEM
HHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHHHH
AEAYLLDQKKTLVCSVMMRGRYGVEDDIFSGIPVIMGSGGVERVIELDLTPDERRMFENS
HHHHHHCCCHHHHHHHHHCCCCCCCHHHHCCCCEEECCCCCEEEEEECCCCHHHHHHHHH
VAATRKLVLEARKYF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA