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The map label for this gene is ruvC

Identifier: 88608702

GI number: 88608702

Start: 839874

End: 840374

Strand: Reverse

Name: ruvC

Synonym: NSE_0955

Alternate gene names: 88608702

Gene position: 840374-839874 (Counterclockwise)

Preceding gene: 88608796

Following gene: 88608823

Centisome position: 97.83

GC content: 44.11

Gene sequence:

>501_bases
ATGAGCTATGTAGCATTAGGGGTTGATCCGGGTCTCCTTCGTACGGGCTGGGCTGTAGTCGAGTATGATGGCCTGTGTAA
CGTGCGTTATATTGATTCCGGAATAGTAAAAACGGCTTCCCAAGGGAGTCTTTCAGCCAGACTAGAAAAGATCCATCGTG
GTATTAGTGATGTGATTGAAAAAGTAAATCCATCTGTTGCCGTTTTAGAGAAGGTATTTGTTAATAACAATCCATATTCT
TCGCTTAACTTAGCTTACTGTCGAGGTGCTCTGATACTTACGCTAGCCCTGAAGGGTCTCTTTATAGTTGAATTCGCGCC
CAGTGTTCTAAAAAAGCGCATAACAGGGAACGGTCGTGCTACTAAGGCTCAGGTTAAGTACATGGTAGAACAACTGCTCG
GGTTGGATCCTTGCTTATCAAAATACTCAGATTTGTATGATGCGCTAGCATTGGCAGCTTCGGTGACTAGATATGACATT
ATGGAAGCAAAGGGCACGTGA

Upstream 100 bases:

>100_bases
AATACTTTTAGAAAGCTGTCTGTTTTTATTGACGGCAAATTAGTGTGTGTAAGGGTTCCTGCATGCGTTTTGTGGTATTT
GAAAAGAATTTTCCTGTTTG

Downstream 100 bases:

>100_bases
GCAAGCCCCTACAACCAAGTATCAGTACTGTATAAAACTCCCATGCGGATGATCTCATTCCTTAGGAGATCCGAAGCAAG
ATGAATTGACTCCCTTAAGG

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSYVALGVDPGLLRTGWAVVEYDGLCNVRYIDSGIVKTASQGSLSARLEKIHRGISDVIEKVNPSVAVLEKVFVNNNPYS
SLNLAYCRGALILTLALKGLFIVEFAPSVLKKRITGNGRATKAQVKYMVEQLLGLDPCLSKYSDLYDALALAASVTRYDI
MEAKGT

Sequences:

>Translated_166_residues
MSYVALGVDPGLLRTGWAVVEYDGLCNVRYIDSGIVKTASQGSLSARLEKIHRGISDVIEKVNPSVAVLEKVFVNNNPYS
SLNLAYCRGALILTLALKGLFIVEFAPSVLKKRITGNGRATKAQVKYMVEQLLGLDPCLSKYSDLYDALALAASVTRYDI
MEAKGT
>Mature_165_residues
SYVALGVDPGLLRTGWAVVEYDGLCNVRYIDSGIVKTASQGSLSARLEKIHRGISDVIEKVNPSVAVLEKVFVNNNPYSS
LNLAYCRGALILTLALKGLFIVEFAPSVLKKRITGNGRATKAQVKYMVEQLLGLDPCLSKYSDLYDALALAASVTRYDIM
EAKGT

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=132, Percent_Identity=34.0909090909091, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_NEOSM (Q2GCH7)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506819.1
- ProteinModelPortal:   Q2GCH7
- SMR:   Q2GCH7
- STRING:   Q2GCH7
- GeneID:   3931536
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0955
- TIGR:   NSE_0955
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- PhylomeDB:   Q2GCH7
- ProtClustDB:   CLSK2528092
- BioCyc:   NSEN222891:NSE_0955-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18019; Mature: 17888

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVALGVDPGLLRTGWAVVEYDGLCNVRYIDSGIVKTASQGSLSARLEKIHRGISDVIE
CCEEEECCCCCHHHCCCEEEEECCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHH
KVNPSVAVLEKVFVNNNPYSSLNLAYCRGALILTLALKGLFIVEFAPSVLKKRITGNGRA
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TKAQVKYMVEQLLGLDPCLSKYSDLYDALALAASVTRYDIMEAKGT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYVALGVDPGLLRTGWAVVEYDGLCNVRYIDSGIVKTASQGSLSARLEKIHRGISDVIE
CEEEECCCCCHHHCCCEEEEECCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHH
KVNPSVAVLEKVFVNNNPYSSLNLAYCRGALILTLALKGLFIVEFAPSVLKKRITGNGRA
HHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TKAQVKYMVEQLLGLDPCLSKYSDLYDALALAASVTRYDIMEAKGT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA