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The map label for this gene is proP [H]

Identifier: 88608756

GI number: 88608756

Start: 694897

End: 696144

Strand: Reverse

Name: proP [H]

Synonym: NSE_0783

Alternate gene names: 88608756

Gene position: 696144-694897 (Counterclockwise)

Preceding gene: 88608641

Following gene: 88608831

Centisome position: 81.04

GC content: 41.03

Gene sequence:

>1248_bases
GTGTCCGAGAAGTCTGGGTTCATTAAACAAATTATTGTAACCCTTCTATGTAACAGCCTCGTGTGGTACGACTATGCACT
TTATGGTCACCTTATAGGTATCATTAATATCTTATTCTTCCCTGAAATTAACCCGGTAACACAACTCCTCTCATCTTTTG
GAACATTTGCTGTTGGTTTCTTGATGAGGCCAATAGGGGCGGTTTTTTTTGGGCACATAGGAGACAAATACGGTAGAAAA
ATTGGGGTTCTGCTATCCCTGATGATGATGTTCTTGCCTGTGGGGATGATAGCGGTTTTACCGACATATCACAGTATTGG
TACGTCGGCGATTGTCCTGCTTATTTTGATTAGATTAATTCAAGGATTTGCTCTTGGTGGGGAAGTTGGAAATGTACTCT
TCTTGATAGAATGCAGTCCTCAGAAGTATAGGGGATTCTTTGGCAGTTTTGAGGTGCTCAGTGCAGTGATAGGTGCTGCA
ATGAGCAGTGTCGTGGTTCTGATATGTAGAAACATAATTCCTGAGGAAGCGTTTTTGAGTTGGGGTTGGAGAGTACCTTT
TGTTATAGGAACATGTGTCGGTGTCTTGGGTGCAGTTCTTAGAAATACACTTGGCGAAACGCCAGTCTTCGAGAGTGTCA
AGAGGTCTGACATCCTACCTGTAACGAAACTCTTTGGTGAATATAAGAAGGCATTTTTTGTTTCAATTGGAATAGACAGT
TTGGAAGAAACAAGTCTGTATCTTTTTCTTGTTTTCTTGGATGTTTATTTTGCAAATAAAATGGGGTTTTCTGGTGCAAC
CGTTGGATTAGTACATCTAATGTTCTTGCTTTTGCTTGCTGTGTTGACCTTGTTTTCGGCGCTACTATCTGATTTCTTCG
GTAGGAGACTAATACTCTCATTAGCTGCAATACTCGCGATTGTGTCTGCGTATCCGATCTTTTTTCTCATAGAAAGCGGG
GGGTTGGGGAATATTATTATTGCAAAAATCTTGTTTGTGGTGATAGTGGGGTTCTCATTGGGGCCCGTGAGCACCGCCGT
GTATGAAATATTTCCTGCTGAGATTTCTTATACAGGACTGGCAATCTCCCGAAATATCTCAAGTGCGCTGTTTGGAGGTA
TGGCACCAGCTTTTACAATGTTTCTTATTGCAGTGACAGGTAATAATCTTTCAGCTGCGATTCCTTTGATTCTTGTTGGG
GTTTTGACTTTAGGTTCTCTATATTTTTATTCAAAAGTATTACACTAG

Upstream 100 bases:

>100_bases
TTCGGGTTTTTATCCTTTGTTTGGACTTGCTCTGGTAATATGCTATATCTAGAAAGTCTAGATTTTAGTTTATCTATCCG
ATCTACTGTAGTAGCGTGCC

Downstream 100 bases:

>100_bases
CTCTACAAAATTGCATTACAATGTACCTCATCTGGTAGTAGGTGTTTCTGACTTTGTATCCAAGGCGGGTTATTTTAAAA
CTATGTCTATTGATGAGAGT

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSEKSGFIKQIIVTLLCNSLVWYDYALYGHLIGIINILFFPEINPVTQLLSSFGTFAVGFLMRPIGAVFFGHIGDKYGRK
IGVLLSLMMMFLPVGMIAVLPTYHSIGTSAIVLLILIRLIQGFALGGEVGNVLFLIECSPQKYRGFFGSFEVLSAVIGAA
MSSVVVLICRNIIPEEAFLSWGWRVPFVIGTCVGVLGAVLRNTLGETPVFESVKRSDILPVTKLFGEYKKAFFVSIGIDS
LEETSLYLFLVFLDVYFANKMGFSGATVGLVHLMFLLLLAVLTLFSALLSDFFGRRLILSLAAILAIVSAYPIFFLIESG
GLGNIIIAKILFVVIVGFSLGPVSTAVYEIFPAEISYTGLAISRNISSALFGGMAPAFTMFLIAVTGNNLSAAIPLILVG
VLTLGSLYFYSKVLH

Sequences:

>Translated_415_residues
MSEKSGFIKQIIVTLLCNSLVWYDYALYGHLIGIINILFFPEINPVTQLLSSFGTFAVGFLMRPIGAVFFGHIGDKYGRK
IGVLLSLMMMFLPVGMIAVLPTYHSIGTSAIVLLILIRLIQGFALGGEVGNVLFLIECSPQKYRGFFGSFEVLSAVIGAA
MSSVVVLICRNIIPEEAFLSWGWRVPFVIGTCVGVLGAVLRNTLGETPVFESVKRSDILPVTKLFGEYKKAFFVSIGIDS
LEETSLYLFLVFLDVYFANKMGFSGATVGLVHLMFLLLLAVLTLFSALLSDFFGRRLILSLAAILAIVSAYPIFFLIESG
GLGNIIIAKILFVVIVGFSLGPVSTAVYEIFPAEISYTGLAISRNISSALFGGMAPAFTMFLIAVTGNNLSAAIPLILVG
VLTLGSLYFYSKVLH
>Mature_414_residues
SEKSGFIKQIIVTLLCNSLVWYDYALYGHLIGIINILFFPEINPVTQLLSSFGTFAVGFLMRPIGAVFFGHIGDKYGRKI
GVLLSLMMMFLPVGMIAVLPTYHSIGTSAIVLLILIRLIQGFALGGEVGNVLFLIECSPQKYRGFFGSFEVLSAVIGAAM
SSVVVLICRNIIPEEAFLSWGWRVPFVIGTCVGVLGAVLRNTLGETPVFESVKRSDILPVTKLFGEYKKAFFVSIGIDSL
EETSLYLFLVFLDVYFANKMGFSGATVGLVHLMFLLLLAVLTLFSALLSDFFGRRLILSLAAILAIVSAYPIFFLIESGG
LGNIIIAKILFVVIVGFSLGPVSTAVYEIFPAEISYTGLAISRNISSALFGGMAPAFTMFLIAVTGNNLSAAIPLILVGV
LTLGSLYFYSKVLH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=409, Percent_Identity=31.0513447432763, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1788292, Length=397, Percent_Identity=31.9899244332494, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=365, Percent_Identity=31.5068493150685, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789941, Length=381, Percent_Identity=30.4461942257218, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44955; Mature: 44823

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKSGFIKQIIVTLLCNSLVWYDYALYGHLIGIINILFFPEINPVTQLLSSFGTFAVGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LMRPIGAVFFGHIGDKYGRKIGVLLSLMMMFLPVGMIAVLPTYHSIGTSAIVLLILIRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGFALGGEVGNVLFLIECSPQKYRGFFGSFEVLSAVIGAAMSSVVVLICRNIIPEEAFLS
HHHCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WGWRVPFVIGTCVGVLGAVLRNTLGETPVFESVKRSDILPVTKLFGEYKKAFFVSIGIDS
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCC
LEETSLYLFLVFLDVYFANKMGFSGATVGLVHLMFLLLLAVLTLFSALLSDFFGRRLILS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAILAIVSAYPIFFLIESGGLGNIIIAKILFVVIVGFSLGPVSTAVYEIFPAEISYTGL
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHCCCCH
AISRNISSALFGGMAPAFTMFLIAVTGNNLSAAIPLILVGVLTLGSLYFYSKVLH
HHHHHHHHHHHHCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKSGFIKQIIVTLLCNSLVWYDYALYGHLIGIINILFFPEINPVTQLLSSFGTFAVGF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LMRPIGAVFFGHIGDKYGRKIGVLLSLMMMFLPVGMIAVLPTYHSIGTSAIVLLILIRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGFALGGEVGNVLFLIECSPQKYRGFFGSFEVLSAVIGAAMSSVVVLICRNIIPEEAFLS
HHHCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
WGWRVPFVIGTCVGVLGAVLRNTLGETPVFESVKRSDILPVTKLFGEYKKAFFVSIGIDS
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCC
LEETSLYLFLVFLDVYFANKMGFSGATVGLVHLMFLLLLAVLTLFSALLSDFFGRRLILS
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAILAIVSAYPIFFLIESGGLGNIIIAKILFVVIVGFSLGPVSTAVYEIFPAEISYTGL
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHCCCCH
AISRNISSALFGGMAPAFTMFLIAVTGNNLSAAIPLILVGVLTLGSLYFYSKVLH
HHHHHHHHHHHHCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]