Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 88608741

GI number: 88608741

Start: 636509

End: 638161

Strand: Reverse

Name: trmE

Synonym: NSE_0717

Alternate gene names: 88608741

Gene position: 638161-636509 (Counterclockwise)

Preceding gene: 88608737

Following gene: 88608449

Centisome position: 74.29

GC content: 41.2

Gene sequence:

>1653_bases
ATGTCAACGATATACGCGCTTTCTACTGTTTTTGGGAAGTCAGGCGTAGCTGTTTTTAGAATTTCTGGGCCAGACGCACT
GCGGGCTCTAGAACTCCTTGGGTTAGATATTAAGCAGCCCCGTCCTAGATTTGTGTACTTTGCAAGACTTTTCGATGAGC
AACTCCTAATCGATGAGGTATTGGTTGTTTATTTTGCTTCTCCTGCGAGTTTTACTGGTGAGGATGTTGTTGAGCTGCAT
TCACATGGTAGCATAGCAGTTCTGCGGTATATCAGTGAGAAGCTTTCTACTTTGTTCAAACCTGCTGAACCGGGTGAGTT
TACCAGGAGAGCAGTTCTGAATAACAGAATGGATCTCACTAAAGCAGAGGGCATAATAGATATAATTAACTCTGAGACCC
AAGAGCAATTAAAACAAGCCTCTCGGCATTTATCGGGGAAATTAGCTGAGGAATATAACTCTCTACGCGATAAAATAATC
AAAGTACTTTCGTATTTGGAGGCTTATATAGACTTTCCAGATGAAGAAATACCAGAAACTGTACTTGCAGAGATACAACA
ATCGATTGTTGCTATACAATGCGACATTTCTCGTTATCTTGCTGACGGTAAAGTTGGAGAAAAAATTAGAGAAGGTTTCT
CGGTTGTAATAGTTGGGAAACCCAATGTAGGTAAGTCGACGCTTTTTAACTATCTTGCTAAGAGGGATCTTGCAATTGTT
ACCGATATTCCAGGTACTACAAGAGACATACTGGAGGTAAGACTAGATTGTCATGGTTATCCAGTGATCCTATCAGATAC
GGCCGGAATCCAGGAAACCTGTGATGCAATAGAAAAAATGGGTATAACTAGGGCGCTCAAAAAAGCAACCGAAGCAGATG
TAATTGTCTTTCTCAGGGATATAACCGAATTGCATCTCACAAATGTTAGACGCGAAGATGTAGCAAACTCAGTCATTGAA
CGACATCTGGATAGGCAAGTAGCAGCTTTGGGAAGTAGAGAGCACATACAAATTGATCAAAGACATGTAGATAAGCTTAT
AGGTTCAAGTGACTGGGCGCAGGAAATGCAATTGAACGAGGCGGGTGTTAGAAGTAAGAATATGAACTACCCGGATATTG
AGGAGAGGGATCTCAATTTGCGAGATATTCTTGATGAACGGCATGAAGAGAAAGACCGTAGTGTGATGTGTCACGACATG
ATGCAGGAGCACGATCCAGATAATGGTGAGTGTAGCGATGATGTCGATAGAAGATTCAATGAATTATTTACAGCTGCAAA
AAAGCTAGAAATTCCTGTAGTAAAAGTTGTGACGAAGGGTGACATTGCACCCACACAGTTGTCATTTTGGCAAGATAAGG
GGTACATATGCATATCCGTATATAAAGGAGAAGGTATGCAGCTTCTTCTTGATAAGATCTTTGATATTATCTCGTCTTCA
AATATTGAAGCTCACATTATTACACGTGCGCGGCATCGACTGGCATTGGAGAATGCTTTAGAGCATCTGCGACGTTTTAA
TACAGATCTCCCAATCGAGCTTGCAGCAGAGGAAATCAAGCTTGCAGCTAATCATATCGCATCTGTTACAGGTGAGATTA
AGTTGGATGATGTCTTGGATGAGATTTTTTCCTCGTTTTGCATAGGTAAATGA

Upstream 100 bases:

>100_bases
GTTTGGTGTGCTTAGAGTCGATGTAGCGAAGGCTTTAATAGAAGGAAAAGGAGATAAGGTGAAGACCGTAAAGTTCGGTA
TAGTGTCACCATTTTAACGA

Downstream 100 bases:

>100_bases
GATCATGTTGTTGTTTGTTTAGTGTATTGCTTGTACTGGTGCAAGGAATGTTGTCATACCAGGTAAAATCTTTCTTTTCC
GTTTAAGAAATTGTCTTTTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSTIYALSTVFGKSGVAVFRISGPDALRALELLGLDIKQPRPRFVYFARLFDEQLLIDEVLVVYFASPASFTGEDVVELH
SHGSIAVLRYISEKLSTLFKPAEPGEFTRRAVLNNRMDLTKAEGIIDIINSETQEQLKQASRHLSGKLAEEYNSLRDKII
KVLSYLEAYIDFPDEEIPETVLAEIQQSIVAIQCDISRYLADGKVGEKIREGFSVVIVGKPNVGKSTLFNYLAKRDLAIV
TDIPGTTRDILEVRLDCHGYPVILSDTAGIQETCDAIEKMGITRALKKATEADVIVFLRDITELHLTNVRREDVANSVIE
RHLDRQVAALGSREHIQIDQRHVDKLIGSSDWAQEMQLNEAGVRSKNMNYPDIEERDLNLRDILDERHEEKDRSVMCHDM
MQEHDPDNGECSDDVDRRFNELFTAAKKLEIPVVKVVTKGDIAPTQLSFWQDKGYICISVYKGEGMQLLLDKIFDIISSS
NIEAHIITRARHRLALENALEHLRRFNTDLPIELAAEEIKLAANHIASVTGEIKLDDVLDEIFSSFCIGK

Sequences:

>Translated_550_residues
MSTIYALSTVFGKSGVAVFRISGPDALRALELLGLDIKQPRPRFVYFARLFDEQLLIDEVLVVYFASPASFTGEDVVELH
SHGSIAVLRYISEKLSTLFKPAEPGEFTRRAVLNNRMDLTKAEGIIDIINSETQEQLKQASRHLSGKLAEEYNSLRDKII
KVLSYLEAYIDFPDEEIPETVLAEIQQSIVAIQCDISRYLADGKVGEKIREGFSVVIVGKPNVGKSTLFNYLAKRDLAIV
TDIPGTTRDILEVRLDCHGYPVILSDTAGIQETCDAIEKMGITRALKKATEADVIVFLRDITELHLTNVRREDVANSVIE
RHLDRQVAALGSREHIQIDQRHVDKLIGSSDWAQEMQLNEAGVRSKNMNYPDIEERDLNLRDILDERHEEKDRSVMCHDM
MQEHDPDNGECSDDVDRRFNELFTAAKKLEIPVVKVVTKGDIAPTQLSFWQDKGYICISVYKGEGMQLLLDKIFDIISSS
NIEAHIITRARHRLALENALEHLRRFNTDLPIELAAEEIKLAANHIASVTGEIKLDDVLDEIFSSFCIGK
>Mature_549_residues
STIYALSTVFGKSGVAVFRISGPDALRALELLGLDIKQPRPRFVYFARLFDEQLLIDEVLVVYFASPASFTGEDVVELHS
HGSIAVLRYISEKLSTLFKPAEPGEFTRRAVLNNRMDLTKAEGIIDIINSETQEQLKQASRHLSGKLAEEYNSLRDKIIK
VLSYLEAYIDFPDEEIPETVLAEIQQSIVAIQCDISRYLADGKVGEKIREGFSVVIVGKPNVGKSTLFNYLAKRDLAIVT
DIPGTTRDILEVRLDCHGYPVILSDTAGIQETCDAIEKMGITRALKKATEADVIVFLRDITELHLTNVRREDVANSVIER
HLDRQVAALGSREHIQIDQRHVDKLIGSSDWAQEMQLNEAGVRSKNMNYPDIEERDLNLRDILDERHEEKDRSVMCHDMM
QEHDPDNGECSDDVDRRFNELFTAAKKLEIPVVKVVTKGDIAPTQLSFWQDKGYICISVYKGEGMQLLLDKIFDIISSSN
IEAHIITRARHRLALENALEHLRRFNTDLPIELAAEEIKLAANHIASVTGEIKLDDVLDEIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=308, Percent_Identity=40.5844155844156, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI193082993, Length=289, Percent_Identity=41.522491349481, Blast_Score=231, Evalue=2e-60,
Organism=Homo sapiens, GI193082988, Length=340, Percent_Identity=36.7647058823529, Blast_Score=226, Evalue=6e-59,
Organism=Escherichia coli, GI2367268, Length=304, Percent_Identity=39.1447368421053, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082120, Length=86, Percent_Identity=45.3488372093023, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=304, Percent_Identity=37.828947368421, Blast_Score=181, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=330, Percent_Identity=36.969696969697, Blast_Score=199, Evalue=9e-52,
Organism=Drosophila melanogaster, GI45550813, Length=303, Percent_Identity=41.9141914191419, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_NEOSM (Q2GD53)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506593.1
- ProteinModelPortal:   Q2GD53
- SMR:   Q2GD53
- STRING:   Q2GD53
- GeneID:   3931628
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0717
- TIGR:   NSE_0717
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   SHGSIAV
- PhylomeDB:   Q2GD53
- ProtClustDB:   CLSK2528116
- BioCyc:   NSEN222891:NSE_0717-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR003593
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- SMART:   SM00382
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 62020; Mature: 61888

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 78-78 BINDING 116-116 BINDING 550-550

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIYALSTVFGKSGVAVFRISGPDALRALELLGLDIKQPRPRFVYFARLFDEQLLIDEV
CCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVVYFASPASFTGEDVVELHSHGSIAVLRYISEKLSTLFKPAEPGEFTRRAVLNNRMDLT
HHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHH
KAEGIIDIINSETQEQLKQASRHLSGKLAEEYNSLRDKIIKVLSYLEAYIDFPDEEIPET
HHCCHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLAEIQQSIVAIQCDISRYLADGKVGEKIREGFSVVIVGKPNVGKSTLFNYLAKRDLAIV
HHHHHHHHHEEEEECHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEE
TDIPGTTRDILEVRLDCHGYPVILSDTAGIQETCDAIEKMGITRALKKATEADVIVFLRD
ECCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ITELHLTNVRREDVANSVIERHLDRQVAALGSREHIQIDQRHVDKLIGSSDWAQEMQLNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHH
AGVRSKNMNYPDIEERDLNLRDILDERHEEKDRSVMCHDMMQEHDPDNGECSDDVDRRFN
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ELFTAAKKLEIPVVKVVTKGDIAPTQLSFWQDKGYICISVYKGEGMQLLLDKIFDIISSS
HHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCC
NIEAHIITRARHRLALENALEHLRRFNTDLPIELAAEEIKLAANHIASVTGEIKLDDVLD
CCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHH
EIFSSFCIGK
HHHHHHCCCC
>Mature Secondary Structure 
STIYALSTVFGKSGVAVFRISGPDALRALELLGLDIKQPRPRFVYFARLFDEQLLIDEV
CHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVVYFASPASFTGEDVVELHSHGSIAVLRYISEKLSTLFKPAEPGEFTRRAVLNNRMDLT
HHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHH
KAEGIIDIINSETQEQLKQASRHLSGKLAEEYNSLRDKIIKVLSYLEAYIDFPDEEIPET
HHCCHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VLAEIQQSIVAIQCDISRYLADGKVGEKIREGFSVVIVGKPNVGKSTLFNYLAKRDLAIV
HHHHHHHHHEEEEECHHHHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEE
TDIPGTTRDILEVRLDCHGYPVILSDTAGIQETCDAIEKMGITRALKKATEADVIVFLRD
ECCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ITELHLTNVRREDVANSVIERHLDRQVAALGSREHIQIDQRHVDKLIGSSDWAQEMQLNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHH
AGVRSKNMNYPDIEERDLNLRDILDERHEEKDRSVMCHDMMQEHDPDNGECSDDVDRRFN
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ELFTAAKKLEIPVVKVVTKGDIAPTQLSFWQDKGYICISVYKGEGMQLLLDKIFDIISSS
HHHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCC
NIEAHIITRARHRLALENALEHLRRFNTDLPIELAAEEIKLAANHIASVTGEIKLDDVLD
CCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHH
EIFSSFCIGK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA