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The map label for this gene is folC

Identifier: 88608449

GI number: 88608449

Start: 634781

End: 636109

Strand: Reverse

Name: folC

Synonym: NSE_0715

Alternate gene names: 88608449

Gene position: 636109-634781 (Counterclockwise)

Preceding gene: 88608741

Following gene: 88608063

Centisome position: 74.05

GC content: 41.76

Gene sequence:

>1329_bases
GTGTATTTCTGGTACCTGATGCCTGTCTATATGCCGCATTGGCCCAAGCCACTAGGTGCTCGGCCTACTGAAATTGATCT
GAAATTACATAGAGTAACACGTGTCTTGAAAAGGCTTGGTAGTCCACATTTGTCTGTGCCCCCAGTCATACATGTTGCTG
GTACCAATGGTAAGGGTTCCACGATAGCATTTCTTCGAGCAATACTTCAGGCTTCAGGTTATTCGGTGAATGTTTACACG
TCACCCCACCTAAGATACTTTAACGAACGAATAGTGCTTCGTAACAGAGAAATAAGTGACAAGTTTCTCTACGACGTTTT
AGAAGATGTGAGAATTGCTTCCCAGGGTGAAAAATTAACTTTTTTTGAAGGGACCACACTTGCTGCGATTTTGGCTTTCT
CGCGTAGTAATGCTGATTTTTCTCTGATTGAAACAGGTATGGGCGGTAGGTTAGATGCAACAAATGTTTTTCCTAAGCCA
ATAGCGACAATTATCACGGCAATAGATTTTGATCATACTTCTTATTTAGGCGCAACTATTGCTCAAATTGCCTATGAAAA
AGCTGGTATTATAAAGGAAGGAGTTCCCTGCGTTGTTGCAAGACAACATGGTGAAGCTATAGACATCATACAGGGTGTTG
CCAAGAGTAAAAGAGTTCCTGTTTTTAGGCAAGGGCATGAGTGGGAGGTTGGAACTGATGGGAGGATGTTACAGTTTGGT
GTTAGTTCACATGTGGAAGAATATCCACTTCCCAACTTGAGAGGCCTACACCAAATTGGGAACGCAGGTAATGCAATTGC
TGCAGCAACAATTTTGAGTAGTAGGTATAACTGCGATGCAATTAGTGTTGAAAGTGTTAGTATTGGTATTAAAAATGCGG
ACTGGCCAGCTAGATTGCAGTTGATACGTGGCAAGAAGAATAAGCTTGTTGGAATGCTTCCAGAAGGCTGGGAGTTCTAC
ATGGATGGAGCACATAACCCTTCTGGAGCTAAAACTATTTCTGAGTGGTTGGAGAAAGAGAAGATTCATATCATTCTTGG
TATGACGAGAGATAAGGATACAAAAGAGTTTGTATCTCATCTCCTCCCGAATATCGAGAGCATCTCAGTAGTATGTGTAC
AAGGTGAGCCACGTAGTAAGACGATTGAAGAAATCGCACAACCCATTCTTGAGCTAGGCTTGGAATGTAGAAAGAGCTCT
TCTCTCGAAGATGCATTTTATCATATTCTCAGTAGAGATTCAGATAGCAAGAAGATTTTGTTATGTGGATCACTATTTCT
TTTTCGTGACATCTGCAGTTATGGTGAGATCGAGGGCTGTCTCTGTTAA

Upstream 100 bases:

>100_bases
CATCAACATTGCAACTATCTTTTGTGCATTAGTTATTGCACTAAAATGATTTATGGTACGAAGCAGGTGCATCTGTGCTA
AACTCTCATCGCTTTTTTAT

Downstream 100 bases:

>100_bases
GCTGCTTCTCTCGAATAAAGTGACTGGTCCCCCCTATTATCTCTGAGCAAACAGGTAATATCACCCTTTTTGACGTGCTT
CTATCTCGTCCCTGAAGTTT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MYFWYLMPVYMPHWPKPLGARPTEIDLKLHRVTRVLKRLGSPHLSVPPVIHVAGTNGKGSTIAFLRAILQASGYSVNVYT
SPHLRYFNERIVLRNREISDKFLYDVLEDVRIASQGEKLTFFEGTTLAAILAFSRSNADFSLIETGMGGRLDATNVFPKP
IATIITAIDFDHTSYLGATIAQIAYEKAGIIKEGVPCVVARQHGEAIDIIQGVAKSKRVPVFRQGHEWEVGTDGRMLQFG
VSSHVEEYPLPNLRGLHQIGNAGNAIAAATILSSRYNCDAISVESVSIGIKNADWPARLQLIRGKKNKLVGMLPEGWEFY
MDGAHNPSGAKTISEWLEKEKIHIILGMTRDKDTKEFVSHLLPNIESISVVCVQGEPRSKTIEEIAQPILELGLECRKSS
SLEDAFYHILSRDSDSKKILLCGSLFLFRDICSYGEIEGCLC

Sequences:

>Translated_442_residues
MYFWYLMPVYMPHWPKPLGARPTEIDLKLHRVTRVLKRLGSPHLSVPPVIHVAGTNGKGSTIAFLRAILQASGYSVNVYT
SPHLRYFNERIVLRNREISDKFLYDVLEDVRIASQGEKLTFFEGTTLAAILAFSRSNADFSLIETGMGGRLDATNVFPKP
IATIITAIDFDHTSYLGATIAQIAYEKAGIIKEGVPCVVARQHGEAIDIIQGVAKSKRVPVFRQGHEWEVGTDGRMLQFG
VSSHVEEYPLPNLRGLHQIGNAGNAIAAATILSSRYNCDAISVESVSIGIKNADWPARLQLIRGKKNKLVGMLPEGWEFY
MDGAHNPSGAKTISEWLEKEKIHIILGMTRDKDTKEFVSHLLPNIESISVVCVQGEPRSKTIEEIAQPILELGLECRKSS
SLEDAFYHILSRDSDSKKILLCGSLFLFRDICSYGEIEGCLC
>Mature_442_residues
MYFWYLMPVYMPHWPKPLGARPTEIDLKLHRVTRVLKRLGSPHLSVPPVIHVAGTNGKGSTIAFLRAILQASGYSVNVYT
SPHLRYFNERIVLRNREISDKFLYDVLEDVRIASQGEKLTFFEGTTLAAILAFSRSNADFSLIETGMGGRLDATNVFPKP
IATIITAIDFDHTSYLGATIAQIAYEKAGIIKEGVPCVVARQHGEAIDIIQGVAKSKRVPVFRQGHEWEVGTDGRMLQFG
VSSHVEEYPLPNLRGLHQIGNAGNAIAAATILSSRYNCDAISVESVSIGIKNADWPARLQLIRGKKNKLVGMLPEGWEFY
MDGAHNPSGAKTISEWLEKEKIHIILGMTRDKDTKEFVSHLLPNIESISVVCVQGEPRSKTIEEIAQPILELGLECRKSS
SLEDAFYHILSRDSDSKKILLCGSLFLFRDICSYGEIEGCLC

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=364, Percent_Identity=27.7472527472527, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI66932984, Length=364, Percent_Identity=27.7472527472527, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1788654, Length=406, Percent_Identity=29.8029556650246, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71984923, Length=340, Percent_Identity=28.8235294117647, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=345, Percent_Identity=28.4057971014493, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=345, Percent_Identity=28.4057971014493, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=426, Percent_Identity=31.924882629108, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=371, Percent_Identity=27.2237196765499, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24641571, Length=332, Percent_Identity=28.0120481927711, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581568, Length=191, Percent_Identity=29.3193717277487, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49113; Mature: 49113

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFWYLMPVYMPHWPKPLGARPTEIDLKLHRVTRVLKRLGSPHLSVPPVIHVAGTNGKGS
CEEEEEEHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCH
TIAFLRAILQASGYSVNVYTSPHLRYFNERIVLRNREISDKFLYDVLEDVRIASQGEKLT
HHHHHHHHHHHCCCEEEEEECCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
FFEGTTLAAILAFSRSNADFSLIETGMGGRLDATNVFPKPIATIITAIDFDHTSYLGATI
EECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
AQIAYEKAGIIKEGVPCVVARQHGEAIDIIQGVAKSKRVPVFRQGHEWEVGTDGRMLQFG
HHHHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCEECCCCEECCCCCCEEEEC
VSSHVEEYPLPNLRGLHQIGNAGNAIAAATILSSRYNCDAISVESVSIGIKNADWPARLQ
HHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHH
LIRGKKNKLVGMLPEGWEFYMDGAHNPSGAKTISEWLEKEKIHIILGMTRDKDTKEFVSH
EECCCCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
LLPNIESISVVCVQGEPRSKTIEEIAQPILELGLECRKSSSLEDAFYHILSRDSDSKKIL
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHCCCCCCCEEE
LCGSLFLFRDICSYGEIEGCLC
EEHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYFWYLMPVYMPHWPKPLGARPTEIDLKLHRVTRVLKRLGSPHLSVPPVIHVAGTNGKGS
CEEEEEEHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCH
TIAFLRAILQASGYSVNVYTSPHLRYFNERIVLRNREISDKFLYDVLEDVRIASQGEKLT
HHHHHHHHHHHCCCEEEEEECCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEE
FFEGTTLAAILAFSRSNADFSLIETGMGGRLDATNVFPKPIATIITAIDFDHTSYLGATI
EECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
AQIAYEKAGIIKEGVPCVVARQHGEAIDIIQGVAKSKRVPVFRQGHEWEVGTDGRMLQFG
HHHHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHCCCCCEECCCCEECCCCCCEEEEC
VSSHVEEYPLPNLRGLHQIGNAGNAIAAATILSSRYNCDAISVESVSIGIKNADWPARLQ
HHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHH
LIRGKKNKLVGMLPEGWEFYMDGAHNPSGAKTISEWLEKEKIHIILGMTRDKDTKEFVSH
EECCCCCCEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
LLPNIESISVVCVQGEPRSKTIEEIAQPILELGLECRKSSSLEDAFYHILSRDSDSKKIL
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHCCCCCCCEEE
LCGSLFLFRDICSYGEIEGCLC
EEHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]