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The map label for this gene is nrdF

Identifier: 88608725

GI number: 88608725

Start: 443212

End: 444180

Strand: Reverse

Name: nrdF

Synonym: NSE_0520

Alternate gene names: 88608725

Gene position: 444180-443212 (Counterclockwise)

Preceding gene: 88607977

Following gene: 88608371

Centisome position: 51.71

GC content: 40.66

Gene sequence:

>969_bases
ATGTCCCTGTTAGAGCCCCGTCTCTCGTATAAACCGTTTCAGTATGATTGGGCCTACCAAGCTTGGGAAATACAACAGAA
GATCCATTGGTTGCCAGAAGAGATTCCTATGGCTGATGATGTGCAGGATTGGCATCATAAGATATGTGGTACAGAGAAAA
ATTTACTGACTCAGATTTTTCGCTTTTTTACCCAAGCAGATGTTGAAGTTCACGATTGTTACATGAGGCACTATGCTGGT
GTTTTCAAGCCGCCGGAAGTCTGTATGATGCTTACTGCTTTTGCAAATATGGAAACTATACATATAGCTGCTTATGCACA
CTTACTAGACACGGTTGGTATGCCGGAAACGGAGTACCTTGCCTTCACAAAGTACAAACAAATGAAGGAAAAATGCGAGT
ATCTGAAGGCTTTCAAGATGGACAATCCAACTGAAATAGCAAAGACACTTGCTGTGTATGGGGCATTTACTGAGGGACTT
CAATTATTTGCATCGTTTGCGATTCTATTAAACTTTCCGCGCTTCAATAAGATGAAGGGTATGGGGCAAATTATTGCCTA
TTCTGTTCGTGATGAAACGCTGCATTCTAACTCGATTACTAAGCTTTTCCGTACTTTTCTGCTTGAGAACAATATCGACA
AGTTTAAATTAGAAGCAGAGATCAGAGAGATATGTAATGCTATGGTTCATCATGAGGATGCGTTTATAGATCTTGCTTTC
GAGATGGGAGATGTGGAAGGTTTAAAGGCATCTGAAGTAAAGGAATACATCAGATACATTGCAAATCTACGGCTGAAGCA
GTTGGGTTTTTCAGAGATATATGAGCAGAAAGTGAATCCTTTGCTCTGGTTAGAGGCGGTACTGAATACAGTCGAGCACA
CTAACTTCTTTGAAAATAAAGCTACCGAATATTCTAAGGCTGCAACAAAAGGTACCTGGGACGAGGCATTCAATTTTAGT
GATGCGTAA

Upstream 100 bases:

>100_bases
CGATTGAATGAGCAATTTATTGCGTACATAATCCCTTGAATTTTGTGAATCAATTTGAGACAATGGCACTCCTCCTTTCT
TCCCTTATTCGAGGTGCGAT

Downstream 100 bases:

>100_bases
AGGTTACATATTACCGATATTTTCTCTGATTGCGTTTTGGCTGATTATTTCAGGGCAAAAGTCGATGTTCCTTTTTGTTC
TTGGATTGGGTGCTACAGCG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSLLEPRLSYKPFQYDWAYQAWEIQQKIHWLPEEIPMADDVQDWHHKICGTEKNLLTQIFRFFTQADVEVHDCYMRHYAG
VFKPPEVCMMLTAFANMETIHIAAYAHLLDTVGMPETEYLAFTKYKQMKEKCEYLKAFKMDNPTEIAKTLAVYGAFTEGL
QLFASFAILLNFPRFNKMKGMGQIIAYSVRDETLHSNSITKLFRTFLLENNIDKFKLEAEIREICNAMVHHEDAFIDLAF
EMGDVEGLKASEVKEYIRYIANLRLKQLGFSEIYEQKVNPLLWLEAVLNTVEHTNFFENKATEYSKAATKGTWDEAFNFS
DA

Sequences:

>Translated_322_residues
MSLLEPRLSYKPFQYDWAYQAWEIQQKIHWLPEEIPMADDVQDWHHKICGTEKNLLTQIFRFFTQADVEVHDCYMRHYAG
VFKPPEVCMMLTAFANMETIHIAAYAHLLDTVGMPETEYLAFTKYKQMKEKCEYLKAFKMDNPTEIAKTLAVYGAFTEGL
QLFASFAILLNFPRFNKMKGMGQIIAYSVRDETLHSNSITKLFRTFLLENNIDKFKLEAEIREICNAMVHHEDAFIDLAF
EMGDVEGLKASEVKEYIRYIANLRLKQLGFSEIYEQKVNPLLWLEAVLNTVEHTNFFENKATEYSKAATKGTWDEAFNFS
DA
>Mature_321_residues
SLLEPRLSYKPFQYDWAYQAWEIQQKIHWLPEEIPMADDVQDWHHKICGTEKNLLTQIFRFFTQADVEVHDCYMRHYAGV
FKPPEVCMMLTAFANMETIHIAAYAHLLDTVGMPETEYLAFTKYKQMKEKCEYLKAFKMDNPTEIAKTLAVYGAFTEGLQ
LFASFAILLNFPRFNKMKGMGQIIAYSVRDETLHSNSITKLFRTFLLENNIDKFKLEAEIREICNAMVHHEDAFIDLAFE
MGDVEGLKASEVKEYIRYIANLRLKQLGFSEIYEQKVNPLLWLEAVLNTVEHTNFFENKATEYSKAATKGTWDEAFNFSD
A

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=311, Percent_Identity=25.4019292604502, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI289177074, Length=313, Percent_Identity=25.2396166134185, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI289177076, Length=290, Percent_Identity=25.1724137931034, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4557845, Length=308, Percent_Identity=23.0519480519481, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI260064013, Length=308, Percent_Identity=23.0519480519481, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1789031, Length=317, Percent_Identity=21.7665615141956, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17551968, Length=286, Percent_Identity=25.8741258741259, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322434, Length=315, Percent_Identity=25.3968253968254, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18079279, Length=302, Percent_Identity=24.8344370860927, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 37614; Mature: 37482

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLEPRLSYKPFQYDWAYQAWEIQQKIHWLPEEIPMADDVQDWHHKICGTEKNLLTQIF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHCCCHHHHHHHHH
RFFTQADVEVHDCYMRHYAGVFKPPEVCMMLTAFANMETIHIAAYAHLLDTVGMPETEYL
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHH
AFTKYKQMKEKCEYLKAFKMDNPTEIAKTLAVYGAFTEGLQLFASFAILLNFPRFNKMKG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIIAYSVRDETLHSNSITKLFRTFLLENNIDKFKLEAEIREICNAMVHHEDAFIDLAF
CHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEE
EMGDVEGLKASEVKEYIRYIANLRLKQLGFSEIYEQKVNPLLWLEAVLNTVEHTNFFENK
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ATEYSKAATKGTWDEAFNFSDA
HHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure 
SLLEPRLSYKPFQYDWAYQAWEIQQKIHWLPEEIPMADDVQDWHHKICGTEKNLLTQIF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHCCCHHHHHHHHH
RFFTQADVEVHDCYMRHYAGVFKPPEVCMMLTAFANMETIHIAAYAHLLDTVGMPETEYL
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHH
AFTKYKQMKEKCEYLKAFKMDNPTEIAKTLAVYGAFTEGLQLFASFAILLNFPRFNKMKG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
MGQIIAYSVRDETLHSNSITKLFRTFLLENNIDKFKLEAEIREICNAMVHHEDAFIDLAF
CHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEE
EMGDVEGLKASEVKEYIRYIANLRLKQLGFSEIYEQKVNPLLWLEAVLNTVEHTNFFENK
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ATEYSKAATKGTWDEAFNFSDA
HHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]