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The map label for this gene is polA

Identifier: 88608724

GI number: 88608724

Start: 590702

End: 593191

Strand: Direct

Name: polA

Synonym: NSE_0675

Alternate gene names: 88608724

Gene position: 590702-593191 (Clockwise)

Preceding gene: 88608170

Following gene: 88608616

Centisome position: 68.77

GC content: 38.35

Gene sequence:

>2490_bases
ATGTCTGAAACCTTGATGATAATAGACGCATATAACTTCCTTTTTAGTGCGTACTTTGCTCTACCAAACCTTACACATAA
TGAACTGCCCGTAGGAGCAGTATACGGTTTCCTAAACATATTGCTAAAACACCTTCGAACCAAACCAGAATATTTAGTGG
TTGCGCTGGATCATGGCAAATCGACATTTAGGGAGACTATATACAACAACTACAAAGCAAACAGACCTCCAGTTCCTGGA
AACCTGACCCCACAGTTCGCTCTTCTTAGGGAATGTGTAGACGTTTTAAATCTTAATCCACTCGAAATTGAAGGCTTTGA
AGCTGATGATGTCATAGCAACTCTGGTAAGAAAGTTTTCTAAAAGTGACCTAAAAATAAAAATACTTTCAACCGACAAGG
ATCTTATGCAACTTGTCAATGAGAATGTACATGTCATAAACCCAATAAAGAACAGGATCATAGGGGTTAACGAAGTTAAG
GAAAAATTTGGTGTATTTCCAACACAAATGACAGATTTTCTCTCCCTTGTTGGTGACACATCTGATAATATTCCAGGAGT
GCCTGGAATTGGTGTAAAAACAGCTGCAAAACTTCTTAACACGTTCGGTGCAATAGAAAACTTCAGACTTAACGAAATTT
CCCCAGCAAGAATTAAAGAAAGTCTTCTTAGACATCACGATCAGCTTACACTATCAAAGAGACTGGTCTCGCTTGTAGAT
GATATAAACATTCAGTGTTCCCTCGATTGCATACGCTATCAGCAACAAGACGGTACAAAACTATTAGCCTTTCTAGAAAA
ATATGGCTTTAAAAACCTTATTCCAAAGCTAGTCAAAAGTACTCCTAAACAGTTACTACATATTACAAAATCAACAAATA
AAATTAATGATTTTAGTCTTAGTGCCAAATATGAAGGCCAGATAACCATTTATAAAAATGGTGAGAAAACCATTTTTAGT
ACGCGAGAAGCCTCAATCATAGGTGACCTAAACATGCTCCAAGATCTATTTACAGATCGATCAATAAAGAAAATTTTTCA
TAGCGACAACATACCACATCCCGACCCTACAAATGACTCTTTTGAGGATCTAAAGGTAATCGCTTATCCTCTGAATACCT
TGAACACGGATCTTGAGTACTTGTTCTCACACTACCTTGGAGAAACTTCCCGAGAAGCGTTCCATAAAAGACTAAATGAG
CTCTATGAAAAACTATGCAACGCAATGTTCACAGAAAAGGTATTGACCCTCTACTATAAGGTGGACAAACCTCTGATTAA
TGCCATAAAACACATGGAAAAAAATGGCATATCCCTGGACGTAGAACAACTCCTCTCACTATCTACGCAAATATCAGGTA
AATTAGAGGAGATAGAAAGCAAGATTTTTGATATTGCCGGAAAGACTTTTTCTCTGCAATCACCGAAGCAACTCGGGAAG
GTGTTATTCGAAGAAATGCAAATACCTGTGCAGAAAAAGCTAAAAAGTGGTTTGTCCAGTACAGACAACGAAGTGTTAAC
CAGCCTGAGCATTCAGGGATATGAGATAGCAGATTACCTTCTCTCGTGGAGACATTATATTAAACTGAAAACTGCGTACA
TAGATCCACTTCTTCTGAAGGTAAATCCCAACGAAGAAAAACATCGTATTTCCACTACTTATTCTGCAACACTTACACTC
ACTGGCAGGCTGAGCTCGTCCAATCCAAATCTTCAAAACATACCAAGAGAAAAAGAAATACGAAAAATTTTTACAGCAAA
AGAAGGACATCATTTTTTATGTGCAGATTACTCTCAAATTGAACTAAGGATTCTTGCATACATAGCAAACGTAGAGCGTT
TGAAATGCGCTCTTGCCAGTGGCACAGATCTGCACACACTTACAGCAAGTCAGATTTTTAAAACGGATAAAATTACACCA
GATTTGCGCAACAAAGCCAAGGCAGTTAATTTCGGCATAGTCTATGGAATAACAAACTTCGGATTATCAAAACAGCTCGC
GATCTCAAAAGAGGAGGCAGCCTCATACATACAAAAATATTTCGATGAGTACCCAGAAATCAAAATCTATATGAATCACA
TAAGGAACCAAGCAAGAAAAGATACTTATGTTAAAACGATGATGAACCGTAAATGCCCTATTCAAAATATAAATAGCAAT
AATCGTACGATTTCACTCGTAGCAGAGAGGTCTGCTATAAATGCTCCTATACAGGGTACTGCCGCGGATATAATACGCAC
AGCAATGGTTAGGATGGCACAAGAAAAAGAACTACTCTCACATATGGTAATGCAAGTACACGACGAACTTGTATTCGAGC
TTGAAACAGACGTAATCCAACAACTAGCAAAAAAAATAAAGCGAATAATGGAAGAATCTCACGGAATCCCACTCTTAGTA
GAGCTGAAACACGGACCAAACCTCGGAGAACTGGAGGACTTAAAACTCGAAAGAAGCCAAGCCCCACAACAGGGAGGGCA
AGGAGGATGA

Upstream 100 bases:

>100_bases
GGAGCCAACAGTACAAGCCGAGAATCCTATACAATATAAAACGTGACTAACCATCCATATGTAGTACAATTCACACTCTG
ATACTAGGGAAAGGTACTCA

Downstream 100 bases:

>100_bases
ACTAGAGAACACGTATACAGCGCCTGATCCATCAGTGCAGACTAAAAAGCGACACTCAGCCAGTACAAAAAGACAAAGAG
AAAAAGCCAGACTACGTCAA

Product: DNA polymerase I

Products: NA

Alternate protein names: Pol I [H]

Number of amino acids: Translated: 829; Mature: 828

Protein sequence:

>829_residues
MSETLMIIDAYNFLFSAYFALPNLTHNELPVGAVYGFLNILLKHLRTKPEYLVVALDHGKSTFRETIYNNYKANRPPVPG
NLTPQFALLRECVDVLNLNPLEIEGFEADDVIATLVRKFSKSDLKIKILSTDKDLMQLVNENVHVINPIKNRIIGVNEVK
EKFGVFPTQMTDFLSLVGDTSDNIPGVPGIGVKTAAKLLNTFGAIENFRLNEISPARIKESLLRHHDQLTLSKRLVSLVD
DINIQCSLDCIRYQQQDGTKLLAFLEKYGFKNLIPKLVKSTPKQLLHITKSTNKINDFSLSAKYEGQITIYKNGEKTIFS
TREASIIGDLNMLQDLFTDRSIKKIFHSDNIPHPDPTNDSFEDLKVIAYPLNTLNTDLEYLFSHYLGETSREAFHKRLNE
LYEKLCNAMFTEKVLTLYYKVDKPLINAIKHMEKNGISLDVEQLLSLSTQISGKLEEIESKIFDIAGKTFSLQSPKQLGK
VLFEEMQIPVQKKLKSGLSSTDNEVLTSLSIQGYEIADYLLSWRHYIKLKTAYIDPLLLKVNPNEEKHRISTTYSATLTL
TGRLSSSNPNLQNIPREKEIRKIFTAKEGHHFLCADYSQIELRILAYIANVERLKCALASGTDLHTLTASQIFKTDKITP
DLRNKAKAVNFGIVYGITNFGLSKQLAISKEEAASYIQKYFDEYPEIKIYMNHIRNQARKDTYVKTMMNRKCPIQNINSN
NRTISLVAERSAINAPIQGTAADIIRTAMVRMAQEKELLSHMVMQVHDELVFELETDVIQQLAKKIKRIMEESHGIPLLV
ELKHGPNLGELEDLKLERSQAPQQGGQGG

Sequences:

>Translated_829_residues
MSETLMIIDAYNFLFSAYFALPNLTHNELPVGAVYGFLNILLKHLRTKPEYLVVALDHGKSTFRETIYNNYKANRPPVPG
NLTPQFALLRECVDVLNLNPLEIEGFEADDVIATLVRKFSKSDLKIKILSTDKDLMQLVNENVHVINPIKNRIIGVNEVK
EKFGVFPTQMTDFLSLVGDTSDNIPGVPGIGVKTAAKLLNTFGAIENFRLNEISPARIKESLLRHHDQLTLSKRLVSLVD
DINIQCSLDCIRYQQQDGTKLLAFLEKYGFKNLIPKLVKSTPKQLLHITKSTNKINDFSLSAKYEGQITIYKNGEKTIFS
TREASIIGDLNMLQDLFTDRSIKKIFHSDNIPHPDPTNDSFEDLKVIAYPLNTLNTDLEYLFSHYLGETSREAFHKRLNE
LYEKLCNAMFTEKVLTLYYKVDKPLINAIKHMEKNGISLDVEQLLSLSTQISGKLEEIESKIFDIAGKTFSLQSPKQLGK
VLFEEMQIPVQKKLKSGLSSTDNEVLTSLSIQGYEIADYLLSWRHYIKLKTAYIDPLLLKVNPNEEKHRISTTYSATLTL
TGRLSSSNPNLQNIPREKEIRKIFTAKEGHHFLCADYSQIELRILAYIANVERLKCALASGTDLHTLTASQIFKTDKITP
DLRNKAKAVNFGIVYGITNFGLSKQLAISKEEAASYIQKYFDEYPEIKIYMNHIRNQARKDTYVKTMMNRKCPIQNINSN
NRTISLVAERSAINAPIQGTAADIIRTAMVRMAQEKELLSHMVMQVHDELVFELETDVIQQLAKKIKRIMEESHGIPLLV
ELKHGPNLGELEDLKLERSQAPQQGGQGG
>Mature_828_residues
SETLMIIDAYNFLFSAYFALPNLTHNELPVGAVYGFLNILLKHLRTKPEYLVVALDHGKSTFRETIYNNYKANRPPVPGN
LTPQFALLRECVDVLNLNPLEIEGFEADDVIATLVRKFSKSDLKIKILSTDKDLMQLVNENVHVINPIKNRIIGVNEVKE
KFGVFPTQMTDFLSLVGDTSDNIPGVPGIGVKTAAKLLNTFGAIENFRLNEISPARIKESLLRHHDQLTLSKRLVSLVDD
INIQCSLDCIRYQQQDGTKLLAFLEKYGFKNLIPKLVKSTPKQLLHITKSTNKINDFSLSAKYEGQITIYKNGEKTIFST
REASIIGDLNMLQDLFTDRSIKKIFHSDNIPHPDPTNDSFEDLKVIAYPLNTLNTDLEYLFSHYLGETSREAFHKRLNEL
YEKLCNAMFTEKVLTLYYKVDKPLINAIKHMEKNGISLDVEQLLSLSTQISGKLEEIESKIFDIAGKTFSLQSPKQLGKV
LFEEMQIPVQKKLKSGLSSTDNEVLTSLSIQGYEIADYLLSWRHYIKLKTAYIDPLLLKVNPNEEKHRISTTYSATLTLT
GRLSSSNPNLQNIPREKEIRKIFTAKEGHHFLCADYSQIELRILAYIANVERLKCALASGTDLHTLTASQIFKTDKITPD
LRNKAKAVNFGIVYGITNFGLSKQLAISKEEAASYIQKYFDEYPEIKIYMNHIRNQARKDTYVKTMMNRKCPIQNINSNN
RTISLVAERSAINAPIQGTAADIIRTAMVRMAQEKELLSHMVMQVHDELVFELETDVIQQLAKKIKRIMEESHGIPLLVE
LKHGPNLGELEDLKLERSQAPQQGGQGG

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=522, Percent_Identity=28.735632183908, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI139394648, Length=541, Percent_Identity=25.3234750462107, Blast_Score=155, Evalue=2e-37,
Organism=Escherichia coli, GI1790294, Length=404, Percent_Identity=41.5841584158416, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI226510970, Length=255, Percent_Identity=28.2352941176471, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI86563272, Length=454, Percent_Identity=25.3303964757709, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI17933644, Length=429, Percent_Identity=30.0699300699301, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 94014; Mature: 93883

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLMIIDAYNFLFSAYFALPNLTHNELPVGAVYGFLNILLKHLRTKPEYLVVALDHGK
CCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCH
STFRETIYNNYKANRPPVPGNLTPQFALLRECVDVLNLNPLEIEGFEADDVIATLVRKFS
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHC
KSDLKIKILSTDKDLMQLVNENVHVINPIKNRIIGVNEVKEKFGVFPTQMTDFLSLVGDT
CCCCEEEEECCCHHHHHHHCCCCEEECCHHHHEECHHHHHHHHCCCCHHHHHHHHHHCCC
SDNIPGVPGIGVKTAAKLLNTFGAIENFRLNEISPARIKESLLRHHDQLTLSKRLVSLVD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DINIQCSLDCIRYQQQDGTKLLAFLEKYGFKNLIPKLVKSTPKQLLHITKSTNKINDFSL
CCCCEEEHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEE
SAKYEGQITIYKNGEKTIFSTREASIIGDLNMLQDLFTDRSIKKIFHSDNIPHPDPTNDS
EEEECCEEEEEECCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
FEDLKVIAYPLNTLNTDLEYLFSHYLGETSREAFHKRLNELYEKLCNAMFTEKVLTLYYK
HHCCEEEEEEHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKPLINAIKHMEKNGISLDVEQLLSLSTQISGKLEEIESKIFDIAGKTFSLQSPKQLGK
HCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCHHHHHH
VLFEEMQIPVQKKLKSGLSSTDNEVLTSLSIQGYEIADYLLSWRHYIKLKTAYIDPLLLK
HHHHHHCCCHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECEEEEE
VNPNEEKHRISTTYSATLTLTGRLSSSNPNLQNIPREKEIRKIFTAKEGHHFLCADYSQI
ECCCCHHHEEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHH
ELRILAYIANVERLKCALASGTDLHTLTASQIFKTDKITPDLRNKAKAVNFGIVYGITNF
HHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHEEEEEEEEECCC
GLSKQLAISKEEAASYIQKYFDEYPEIKIYMNHIRNQARKDTYVKTMMNRKCPIQNINSN
CCCHHHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NRTISLVAERSAINAPIQGTAADIIRTAMVRMAQEKELLSHMVMQVHDELVFELETDVIQ
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAKKIKRIMEESHGIPLLVELKHGPNLGELEDLKLERSQAPQQGGQGG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SETLMIIDAYNFLFSAYFALPNLTHNELPVGAVYGFLNILLKHLRTKPEYLVVALDHGK
CCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCH
STFRETIYNNYKANRPPVPGNLTPQFALLRECVDVLNLNPLEIEGFEADDVIATLVRKFS
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHC
KSDLKIKILSTDKDLMQLVNENVHVINPIKNRIIGVNEVKEKFGVFPTQMTDFLSLVGDT
CCCCEEEEECCCHHHHHHHCCCCEEECCHHHHEECHHHHHHHHCCCCHHHHHHHHHHCCC
SDNIPGVPGIGVKTAAKLLNTFGAIENFRLNEISPARIKESLLRHHDQLTLSKRLVSLVD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DINIQCSLDCIRYQQQDGTKLLAFLEKYGFKNLIPKLVKSTPKQLLHITKSTNKINDFSL
CCCCEEEHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEE
SAKYEGQITIYKNGEKTIFSTREASIIGDLNMLQDLFTDRSIKKIFHSDNIPHPDPTNDS
EEEECCEEEEEECCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
FEDLKVIAYPLNTLNTDLEYLFSHYLGETSREAFHKRLNELYEKLCNAMFTEKVLTLYYK
HHCCEEEEEEHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKPLINAIKHMEKNGISLDVEQLLSLSTQISGKLEEIESKIFDIAGKTFSLQSPKQLGK
HCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCHHHHHH
VLFEEMQIPVQKKLKSGLSSTDNEVLTSLSIQGYEIADYLLSWRHYIKLKTAYIDPLLLK
HHHHHHCCCHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECEEEEE
VNPNEEKHRISTTYSATLTLTGRLSSSNPNLQNIPREKEIRKIFTAKEGHHFLCADYSQI
ECCCCHHHEEEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHH
ELRILAYIANVERLKCALASGTDLHTLTASQIFKTDKITPDLRNKAKAVNFGIVYGITNF
HHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHEEEEEEEEECCC
GLSKQLAISKEEAASYIQKYFDEYPEIKIYMNHIRNQARKDTYVKTMMNRKCPIQNINSN
CCCHHHHCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NRTISLVAERSAINAPIQGTAADIIRTAMVRMAQEKELLSHMVMQVHDELVFELETDVIQ
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLAKKIKRIMEESHGIPLLVELKHGPNLGELEDLKLERSQAPQQGGQGG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA