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The map label for this gene is lpdA-2

Identifier: 88608170

GI number: 88608170

Start: 586511

End: 587917

Strand: Direct

Name: lpdA-2

Synonym: NSE_0671

Alternate gene names: 88608170

Gene position: 586511-587917 (Clockwise)

Preceding gene: 88608463

Following gene: 88608724

Centisome position: 68.28

GC content: 44.14

Gene sequence:

>1407_bases
TTGTGTCACTTTTTTGTGAGAATGTCCGAGAAAGAGTTTGATGTAGTTGTCATAGGCGGCGGCCCCGCTGGATACGTGTG
TAGTATAAAAGCGGCACAACTCGGTATGAAGGTTGCATGTGTAGAAAAACGCCCTTCTCTCGGTGGGACATGTCTGAACG
AAGGATGTATCCCTTCCAAGGCGTTACTCCACTCATCCTACGCTTACTATTCTGCTAAAAAGTGCTTTGATGTTCTCGGA
GTAGAATGCTCTGATGTAAAGTTGAACCTAACAAAAATGATGGGAAATAAATCCAGAATAGTGATGGAATTATCCCAAGG
CATCGAGTTTTTATTCAAGAAAAATAAGGTTACTCGGTTTACTGGAACGGGCAGCATACTAGCCAATGGTGATACAAAAA
AAAAATCCGTCATTATTGACAAAACTGAGACCATACACACAAAGTATGTTGTTCTTGCAACTGGTTCCGAGGCAGCTGAG
CTTCCGTTCGCGAAGTGTGATGAGAAAAGCATCTTATCATCACGTGGCGCATTAGAGCTGGACGCTGTCCCTAAAAGCAT
GATTATCATTGGAGGGGGAGCAATAGGACTGGAAATGGCTTCCATTTGGTCAAGACTTGGCACTGAGGTCACCCTTATGG
AATACGCGGACAGAATCGCAGCAGCCTCAGATGGTGAAGTCAGCGACTATCTTCTGAAATCACTAACAAAACAAGGTATA
AAGTTCCATCTCTCCAGTAGGATTACAGAGATAAAAAAAGGAAAGCTTCTTTCTGCAACCTTTGAGAAAGATGAAAAAAT
AGGAAGTATATCAGCAGAAAAAATTCTAGTCGCCGTTGGCAGGCGTCCATACTCAGCCAATATTGGTGTTGAGCTGGAGA
GGAACCCGTCTGGTTTCATAAAGGTAGATAAAAATTTCCAAACCAGCGTTCCTGGAGTATATGCCATAGGGGACACAATT
CCGGGGGTAATGCTAGCTCACAAAGCCGAGGAAGAAGGGGTAGCTGTAGCAGAAATCCTAGCAGGTAGGACAGGCCACAT
TGGATGGATTCCATCTGTAATATATACTCACCCAGAAGTTGCAAGTGTAGGAAAAACCGAGGAAGAATTGAAAGTCATAG
GTATAAAATACAAAGCCTCCAAATTTCCGTTTGCAGCTAATAGTCGGGCTAAAACGACTAACGACACAGGAGGTTTTGTA
AAGATGTTAGTTGATGAGCACGACACCGTACTCGGTGTGCACATAGTCGGACCATCTGCTTCTTCACTTATAGCCGAAGC
CGTCCTGGCAATGGAATATGGTGCTTCAGCGGAGGATATCGCGAGAACCTGCCACTCTCACCCAGACCTAAACGAAGCAA
TGAAAGAAGCCGCTCTAGGAGCATTTTTCAAGCCAATACATTTCTAA

Upstream 100 bases:

>100_bases
AGCAAGTCTGCGCAGCAGAGAACTACCCAAAAAGTAATTTCGGCATAATAAAGAATAGAAAAAGATTTCCAGCACAAAAT
TTTTGTAATACCATTTAGGC

Downstream 100 bases:

>100_bases
TCATTCCAGGTCAGAATTACCTGGTATATGTTTGTTTTATAATCCACTCGCCTGGCGTGCGTTTGTTCGTTGCGTGTCGT
AGAGCAATTTCTCCTCTATC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MCHFFVRMSEKEFDVVVIGGGPAGYVCSIKAAQLGMKVACVEKRPSLGGTCLNEGCIPSKALLHSSYAYYSAKKCFDVLG
VECSDVKLNLTKMMGNKSRIVMELSQGIEFLFKKNKVTRFTGTGSILANGDTKKKSVIIDKTETIHTKYVVLATGSEAAE
LPFAKCDEKSILSSRGALELDAVPKSMIIIGGGAIGLEMASIWSRLGTEVTLMEYADRIAAASDGEVSDYLLKSLTKQGI
KFHLSSRITEIKKGKLLSATFEKDEKIGSISAEKILVAVGRRPYSANIGVELERNPSGFIKVDKNFQTSVPGVYAIGDTI
PGVMLAHKAEEEGVAVAEILAGRTGHIGWIPSVIYTHPEVASVGKTEEELKVIGIKYKASKFPFAANSRAKTTNDTGGFV
KMLVDEHDTVLGVHIVGPSASSLIAEAVLAMEYGASAEDIARTCHSHPDLNEAMKEAALGAFFKPIHF

Sequences:

>Translated_468_residues
MCHFFVRMSEKEFDVVVIGGGPAGYVCSIKAAQLGMKVACVEKRPSLGGTCLNEGCIPSKALLHSSYAYYSAKKCFDVLG
VECSDVKLNLTKMMGNKSRIVMELSQGIEFLFKKNKVTRFTGTGSILANGDTKKKSVIIDKTETIHTKYVVLATGSEAAE
LPFAKCDEKSILSSRGALELDAVPKSMIIIGGGAIGLEMASIWSRLGTEVTLMEYADRIAAASDGEVSDYLLKSLTKQGI
KFHLSSRITEIKKGKLLSATFEKDEKIGSISAEKILVAVGRRPYSANIGVELERNPSGFIKVDKNFQTSVPGVYAIGDTI
PGVMLAHKAEEEGVAVAEILAGRTGHIGWIPSVIYTHPEVASVGKTEEELKVIGIKYKASKFPFAANSRAKTTNDTGGFV
KMLVDEHDTVLGVHIVGPSASSLIAEAVLAMEYGASAEDIARTCHSHPDLNEAMKEAALGAFFKPIHF
>Mature_468_residues
MCHFFVRMSEKEFDVVVIGGGPAGYVCSIKAAQLGMKVACVEKRPSLGGTCLNEGCIPSKALLHSSYAYYSAKKCFDVLG
VECSDVKLNLTKMMGNKSRIVMELSQGIEFLFKKNKVTRFTGTGSILANGDTKKKSVIIDKTETIHTKYVVLATGSEAAE
LPFAKCDEKSILSSRGALELDAVPKSMIIIGGGAIGLEMASIWSRLGTEVTLMEYADRIAAASDGEVSDYLLKSLTKQGI
KFHLSSRITEIKKGKLLSATFEKDEKIGSISAEKILVAVGRRPYSANIGVELERNPSGFIKVDKNFQTSVPGVYAIGDTI
PGVMLAHKAEEEGVAVAEILAGRTGHIGWIPSVIYTHPEVASVGKTEEELKVIGIKYKASKFPFAANSRAKTTNDTGGFV
KMLVDEHDTVLGVHIVGPSASSLIAEAVLAMEYGASAEDIARTCHSHPDLNEAMKEAALGAFFKPIHF

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=472, Percent_Identity=50.8474576271186, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI50301238, Length=471, Percent_Identity=26.3269639065817, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI22035672, Length=487, Percent_Identity=26.0780287474333, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI291045266, Length=462, Percent_Identity=25.974025974026, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI33519430, Length=449, Percent_Identity=24.2761692650334, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI33519428, Length=449, Percent_Identity=24.2761692650334, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI33519426, Length=449, Percent_Identity=24.2761692650334, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI148277065, Length=449, Percent_Identity=24.2761692650334, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI148277071, Length=449, Percent_Identity=24.2761692650334, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI291045268, Length=454, Percent_Identity=25.7709251101322, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=36.480686695279, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=27.5210084033613, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=26.2068965517241, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=24.6252676659529, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=51.0822510822511, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17557007, Length=486, Percent_Identity=27.3662551440329, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=379, Percent_Identity=26.3852242744063, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71983419, Length=379, Percent_Identity=26.3852242744063, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=493, Percent_Identity=25.9634888438134, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=50.4166666666667, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=31.5565031982942, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=26.226012793177, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=52.431289640592, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24640551, Length=497, Percent_Identity=25.9557344064386, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=25.8196721311475, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=25.8196721311475, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=23.1083844580777, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50254; Mature: 50254

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCHFFVRMSEKEFDVVVIGGGPAGYVCSIKAAQLGMKVACVEKRPSLGGTCLNEGCIPSK
CEEEEEEECCCCCCEEEECCCCCCEEEEEEHHHCCEEEEEECCCCCCCCCCCCCCCCCHH
ALLHSSYAYYSAKKCFDVLGVECSDVKLNLTKMMGNKSRIVMELSQGIEFLFKKNKVTRF
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCEEHHHHHHHHHHHHCCCEEEE
TGTGSILANGDTKKKSVIIDKTETIHTKYVVLATGSEAAELPFAKCDEKSILSSRGALEL
ECCCCEEECCCCCCCEEEEECCCEEEEEEEEEEECCCHHCCCCCCCCCHHHHHCCCCEEE
DAVPKSMIIIGGGAIGLEMASIWSRLGTEVTLMEYADRIAAASDGEVSDYLLKSLTKQGI
ECCCCEEEEEECCHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
KFHLSSRITEIKKGKLLSATFEKDEKIGSISAEKILVAVGRRPYSANIGVELERNPSGFI
EEEHHHHHHHHHCCCEEEEHHHCCHHCCCCCHHEEEEEECCCCCCCCCCEEEECCCCCEE
KVDKNFQTSVPGVYAIGDTIPGVMLAHKAEEEGVAVAEILAGRTGHIGWIPSVIYTHPEV
EECCCCCCCCCEEEEECCCCCCHHEEECCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCH
ASVGKTEEELKVIGIKYKASKFPFAANSRAKTTNDTGGFVKMLVDEHDTVLGVHIVGPSA
HHCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCH
SSLIAEAVLAMEYGASAEDIARTCHSHPDLNEAMKEAALGAFFKPIHF
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCHFFVRMSEKEFDVVVIGGGPAGYVCSIKAAQLGMKVACVEKRPSLGGTCLNEGCIPSK
CEEEEEEECCCCCCEEEECCCCCCEEEEEEHHHCCEEEEEECCCCCCCCCCCCCCCCCHH
ALLHSSYAYYSAKKCFDVLGVECSDVKLNLTKMMGNKSRIVMELSQGIEFLFKKNKVTRF
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCEEHHHHHHHHHHHHCCCEEEE
TGTGSILANGDTKKKSVIIDKTETIHTKYVVLATGSEAAELPFAKCDEKSILSSRGALEL
ECCCCEEECCCCCCCEEEEECCCEEEEEEEEEEECCCHHCCCCCCCCCHHHHHCCCCEEE
DAVPKSMIIIGGGAIGLEMASIWSRLGTEVTLMEYADRIAAASDGEVSDYLLKSLTKQGI
ECCCCEEEEEECCHHHHHHHHHHHHHCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
KFHLSSRITEIKKGKLLSATFEKDEKIGSISAEKILVAVGRRPYSANIGVELERNPSGFI
EEEHHHHHHHHHCCCEEEEHHHCCHHCCCCCHHEEEEEECCCCCCCCCCEEEECCCCCEE
KVDKNFQTSVPGVYAIGDTIPGVMLAHKAEEEGVAVAEILAGRTGHIGWIPSVIYTHPEV
EECCCCCCCCCEEEEECCCCCCHHEEECCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCH
ASVGKTEEELKVIGIKYKASKFPFAANSRAKTTNDTGGFVKMLVDEHDTVLGVHIVGPSA
HHCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCH
SSLIAEAVLAMEYGASAEDIARTCHSHPDLNEAMKEAALGAFFKPIHF
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9226266 [H]