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The map label for this gene is truB

Identifier: 88608670

GI number: 88608670

Start: 81266

End: 81973

Strand: Direct

Name: truB

Synonym: NSE_0101

Alternate gene names: 88608670

Gene position: 81266-81973 (Clockwise)

Preceding gene: 88608073

Following gene: 88608495

Centisome position: 9.46

GC content: 44.21

Gene sequence:

>708_bases
ATGACGTTGGATGTGAAATTAGATAGCATAGAAGGTTGGCTTAGCTTGGATAAAGAAAGCGGAATCTCCTCGTTTGGTGC
ATTGAAACTTTTAAAGCGTAAGTTTGGTGTAAAGAAGGCGGGTTATGTAGGAACATTAGACCCTATTGCAACGGGAGTCC
TGGTTATTGCTCTTGGCAGGACAACAAAGCTTATCCCGCAGTTTGATGATTCTTTCAAAGAATACGAATTCGCAATTGAA
TGGGGTGCGGAAACAGATACACTAGATTCGGAGGGTGCAGTGCTTCGAAATGGTGGGCTCATACCAAGTACAAGTGCAAT
TCTTGGGGTTTTACCGTCATTTCTTGGTGAGCAGTTACAAGTGCCACCTAAGTTTTCGGCCATCCACGTAAACGGCCATA
GGGCCTATTCACTCGCCCGAGCTGGTATAGAGTTTAACATGCAACCTAGAAGGGTCGTTATACACAGTATCGAATGCATG
AATCATTCAGCTGGACAATCAACCTTAAAAGTATCATGTAGTAAAGGAACTTATGTCAGGGCGCTCGCTCGGGACATCGC
ACGCAAGCTGGAGTCATTAGCATTTGTAAGCAAAATACGCAGAACAAGATCAGGCATATTCAACGTTACCAGTGGAAACA
AGCTTTCAGAAATTACAAAGGAGCATATAGTCCCACCAAGTGTGCCACTTTGCAGGCCTAAAGGCTAA

Upstream 100 bases:

>100_bases
CTGCAAAGGAAATGGGTATAGAAATAAGGAGCTTTAATGCATTTGATAGATACTTCAAAAAATCATTTGTGAAAATCTTT
GGCGAATGAGTTAGGGTGGT

Downstream 100 bases:

>100_bases
TCACCCCTGCCTACATCAGATGCCCGTGAAACCGACTCTACTGATTCGTTCCTGGAAGTTCCACGTAGGACGAGTGTGAT
TGCAGCCGCCATAAAAATGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTLDVKLDSIEGWLSLDKESGISSFGALKLLKRKFGVKKAGYVGTLDPIATGVLVIALGRTTKLIPQFDDSFKEYEFAIE
WGAETDTLDSEGAVLRNGGLIPSTSAILGVLPSFLGEQLQVPPKFSAIHVNGHRAYSLARAGIEFNMQPRRVVIHSIECM
NHSAGQSTLKVSCSKGTYVRALARDIARKLESLAFVSKIRRTRSGIFNVTSGNKLSEITKEHIVPPSVPLCRPKG

Sequences:

>Translated_235_residues
MTLDVKLDSIEGWLSLDKESGISSFGALKLLKRKFGVKKAGYVGTLDPIATGVLVIALGRTTKLIPQFDDSFKEYEFAIE
WGAETDTLDSEGAVLRNGGLIPSTSAILGVLPSFLGEQLQVPPKFSAIHVNGHRAYSLARAGIEFNMQPRRVVIHSIECM
NHSAGQSTLKVSCSKGTYVRALARDIARKLESLAFVSKIRRTRSGIFNVTSGNKLSEITKEHIVPPSVPLCRPKG
>Mature_234_residues
TLDVKLDSIEGWLSLDKESGISSFGALKLLKRKFGVKKAGYVGTLDPIATGVLVIALGRTTKLIPQFDDSFKEYEFAIEW
GAETDTLDSEGAVLRNGGLIPSTSAILGVLPSFLGEQLQVPPKFSAIHVNGHRAYSLARAGIEFNMQPRRVVIHSIECMN
HSAGQSTLKVSCSKGTYVRALARDIARKLESLAFVSKIRRTRSGIFNVTSGNKLSEITKEHIVPPSVPLCRPKG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=196, Percent_Identity=36.2244897959184, Blast_Score=104, Evalue=6e-23,
Organism=Escherichia coli, GI2367200, Length=193, Percent_Identity=36.2694300518135, Blast_Score=131, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324037, Length=135, Percent_Identity=32.5925925925926, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281364189, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364187, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364185, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364183, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62471759, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17975520, Length=212, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25539; Mature: 25408

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDVKLDSIEGWLSLDKESGISSFGALKLLKRKFGVKKAGYVGTLDPIATGVLVIALGR
CEEEEEECCCCHHEEECHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCEEEEEECC
TTKLIPQFDDSFKEYEFAIEWGAETDTLDSEGAVLRNGGLIPSTSAILGVLPSFLGEQLQ
CCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCC
VPPKFSAIHVNGHRAYSLARAGIEFNMQPRRVVIHSIECMNHSAGQSTLKVSCSKGTYVR
CCCCCEEEEECCCHHHHHHHCCCEECCCCCCCEEEHHHHHCCCCCCCEEEEEECCCHHHH
ALARDIARKLESLAFVSKIRRTRSGIFNVTSGNKLSEITKEHIVPPSVPLCRPKG
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TLDVKLDSIEGWLSLDKESGISSFGALKLLKRKFGVKKAGYVGTLDPIATGVLVIALGR
EEEEEECCCCHHEEECHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCEEEEEECC
TTKLIPQFDDSFKEYEFAIEWGAETDTLDSEGAVLRNGGLIPSTSAILGVLPSFLGEQLQ
CCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCC
VPPKFSAIHVNGHRAYSLARAGIEFNMQPRRVVIHSIECMNHSAGQSTLKVSCSKGTYVR
CCCCCEEEEECCCHHHHHHHCCCEECCCCCCCEEEHHHHHCCCCCCCEEEEEECCCHHHH
ALARDIARKLESLAFVSKIRRTRSGIFNVTSGNKLSEITKEHIVPPSVPLCRPKG
HHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]