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The map label for this gene is lipB

Identifier: 88608073

GI number: 88608073

Start: 80625

End: 81254

Strand: Direct

Name: lipB

Synonym: NSE_0100

Alternate gene names: 88608073

Gene position: 80625-81254 (Clockwise)

Preceding gene: 88608337

Following gene: 88608670

Centisome position: 9.39

GC content: 39.68

Gene sequence:

>630_bases
ATGGAATGGAAAATTGATAAAGACTGTGTTGATTACCAAATGTCACTCATGTTTATGCAAGAGCGGGTGAAGCAGGTTAT
TAACGGTTCAGGCGATGAATTGGTGTGGATGTTGCAATATGAGGCGCTCTATACGGGTGGCACGAGTGCAGATCCACAGG
ATCTACTCAATTCAGATCTTTTCCCAGTTTTCAACGTGGGCAGAGGTGGTAAGTACACTTATCATGGTCCTGGGCAACGT
GTTATCTATCCAATACTTAATCTAAGAAGCCGTAATATATGTGATCTCCATAAATATATTTATTTGTTGGAAGAAGTAGT
AATTGTCACGCTCGATAATTTTGGGATTAATGGCTGTAGAAAAGAGGGGCATATTGGGGTCTGGGTTGGAACGGGATGTC
AGCCACCAAAAAAAATTGCGGCAATTGGTGTAAGAGTGAGTAAGTGGGTAAGTTATCATGGCATTGCTGTTAATTTGTAT
CCAGATCTGAGCCACTATGATGCGATAATTCCCTGCGGAATTAAGAATTTTGGTGTAACTTCTGCAAAGGAAATGGGTAT
AGAAATAAGGAGCTTTAATGCATTTGATAGATACTTCAAAAAATCATTTGTGAAAATCTTTGGCGAATGA

Upstream 100 bases:

>100_bases
TATTTTGACTTTCGATCTCGTAAAAACAGCGAGCTCTGTACAATGTTGATATCATGAATTTGTATAATGACCTCAAGCGG
GTCTGTGTCAATGGGTTATG

Downstream 100 bases:

>100_bases
GTTAGGGTGGTATGACGTTGGATGTGAAATTAGATAGCATAGAAGGTTGGCTTAGCTTGGATAAAGAAAGCGGAATCTCC
TCGTTTGGTGCATTGAAACT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MEWKIDKDCVDYQMSLMFMQERVKQVINGSGDELVWMLQYEALYTGGTSADPQDLLNSDLFPVFNVGRGGKYTYHGPGQR
VIYPILNLRSRNICDLHKYIYLLEEVVIVTLDNFGINGCRKEGHIGVWVGTGCQPPKKIAAIGVRVSKWVSYHGIAVNLY
PDLSHYDAIIPCGIKNFGVTSAKEMGIEIRSFNAFDRYFKKSFVKIFGE

Sequences:

>Translated_209_residues
MEWKIDKDCVDYQMSLMFMQERVKQVINGSGDELVWMLQYEALYTGGTSADPQDLLNSDLFPVFNVGRGGKYTYHGPGQR
VIYPILNLRSRNICDLHKYIYLLEEVVIVTLDNFGINGCRKEGHIGVWVGTGCQPPKKIAAIGVRVSKWVSYHGIAVNLY
PDLSHYDAIIPCGIKNFGVTSAKEMGIEIRSFNAFDRYFKKSFVKIFGE
>Mature_209_residues
MEWKIDKDCVDYQMSLMFMQERVKQVINGSGDELVWMLQYEALYTGGTSADPQDLLNSDLFPVFNVGRGGKYTYHGPGQR
VIYPILNLRSRNICDLHKYIYLLEEVVIVTLDNFGINGCRKEGHIGVWVGTGCQPPKKIAAIGVRVSKWVSYHGIAVNLY
PDLSHYDAIIPCGIKNFGVTSAKEMGIEIRSFNAFDRYFKKSFVKIFGE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=149, Percent_Identity=34.2281879194631, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI87081767, Length=166, Percent_Identity=32.5301204819277, Blast_Score=101, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323268, Length=209, Percent_Identity=28.2296650717703, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355483, Length=216, Percent_Identity=31.4814814814815, Blast_Score=94, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_NEOSM (Q2GEU6)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506000.1
- ProteinModelPortal:   Q2GEU6
- SMR:   Q2GEU6
- STRING:   Q2GEU6
- GeneID:   3931621
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0100
- TIGR:   NSE_0100
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   RKEGHIG
- PhylomeDB:   Q2GEU6
- BioCyc:   NSEN222891:NSE_0100-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23719; Mature: 23719

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWKIDKDCVDYQMSLMFMQERVKQVINGSGDELVWMLQYEALYTGGTSADPQDLLNSDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHCCCC
FPVFNVGRGGKYTYHGPGQRVIYPILNLRSRNICDLHKYIYLLEEVVIVTLDNFGINGCR
CEEEECCCCCCEEEECCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCC
KEGHIGVWVGTGCQPPKKIAAIGVRVSKWVSYHGIAVNLYPDLSHYDAIIPCGIKNFGVT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCCCCCC
SAKEMGIEIRSFNAFDRYFKKSFVKIFGE
CHHHHCEEEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEWKIDKDCVDYQMSLMFMQERVKQVINGSGDELVWMLQYEALYTGGTSADPQDLLNSDL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHCCCC
FPVFNVGRGGKYTYHGPGQRVIYPILNLRSRNICDLHKYIYLLEEVVIVTLDNFGINGCR
CEEEECCCCCCEEEECCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCC
KEGHIGVWVGTGCQPPKKIAAIGVRVSKWVSYHGIAVNLYPDLSHYDAIIPCGIKNFGVT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCCCCCC
SAKEMGIEIRSFNAFDRYFKKSFVKIFGE
CHHHHCEEEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA