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The map label for this gene is pth

Identifier: 88608638

GI number: 88608638

Start: 250381

End: 250965

Strand: Direct

Name: pth

Synonym: NSE_0307

Alternate gene names: 88608638

Gene position: 250381-250965 (Clockwise)

Preceding gene: 88608536

Following gene: 88608590

Centisome position: 29.15

GC content: 42.74

Gene sequence:

>585_bases
ATGTCCGAGTTTCGCACGTTTGTTCTGTGTGGCTTGGGTAACCCGGGACTGCGGTATGCGCAGACGCGGCATAACCTTGG
GTTCATGCTTATTGATTACGTTAGGTATACCTTTTCTTTTCCGGAGTTTCTTCCGAAATTTTCAGGGCTCTTGTCTTCGG
GACGGGTCTCTTCCTTTCTTTTGTACTTATTCAAGCCAGTAGCCTTTATGAATAATTCAGGTAGGCCGCTCGTACAGCTG
GTCAACTTTTATAAAGTTGAGCTGGAGAATGTAATTGTCTTTCATGATGATATAGATCTTGATTTTGCCAAAGTGAAGAT
AAAAAGGGGTGGAGGCTCTGGAGGTCATAATGGACTGAAATCTCTTGATTTTAACTTGGGTAGAGATTATTGGAGGTTTC
GCTTCGGTGTTGGAAGAGGTGTTGGTGACCCGGCTGAACATGTTTTGTCACGTTTTACTGCGTTGGAGTTGGAGCGTCTG
AATAAGTTATTTGGATTTATTGGTACGAATCTTCCTCTTCTTTTAAGTGATATTGCTACGCGCAAAGAAAAGTTCCTCAA
TGAATACAAGTGCTGCAGTCAGTAA

Upstream 100 bases:

>100_bases
TTCCTGATGGAGTTGTGCCACTTGCGAGAGATGCAAATTCGGTTGTTGCGACGGTTGTAGGTAAGAAAGTTAAGGCTGGT
ACTGTGGCTGCTACCGCGTA

Downstream 100 bases:

>100_bases
ATGATAATCCCGAGAAGTAGTGTTATATGGTCTTCCGTAACAGGTCGGTATGGGTACTAATAGCGGTCCGTTATTCTTTT
TCCACATCGAGCAATTTCAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSEFRTFVLCGLGNPGLRYAQTRHNLGFMLIDYVRYTFSFPEFLPKFSGLLSSGRVSSFLLYLFKPVAFMNNSGRPLVQL
VNFYKVELENVIVFHDDIDLDFAKVKIKRGGGSGGHNGLKSLDFNLGRDYWRFRFGVGRGVGDPAEHVLSRFTALELERL
NKLFGFIGTNLPLLLSDIATRKEKFLNEYKCCSQ

Sequences:

>Translated_194_residues
MSEFRTFVLCGLGNPGLRYAQTRHNLGFMLIDYVRYTFSFPEFLPKFSGLLSSGRVSSFLLYLFKPVAFMNNSGRPLVQL
VNFYKVELENVIVFHDDIDLDFAKVKIKRGGGSGGHNGLKSLDFNLGRDYWRFRFGVGRGVGDPAEHVLSRFTALELERL
NKLFGFIGTNLPLLLSDIATRKEKFLNEYKCCSQ
>Mature_193_residues
SEFRTFVLCGLGNPGLRYAQTRHNLGFMLIDYVRYTFSFPEFLPKFSGLLSSGRVSSFLLYLFKPVAFMNNSGRPLVQLV
NFYKVELENVIVFHDDIDLDFAKVKIKRGGGSGGHNGLKSLDFNLGRDYWRFRFGVGRGVGDPAEHVLSRFTALELERLN
KLFGFIGTNLPLLLSDIATRKEKFLNEYKCCSQ

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=166, Percent_Identity=28.9156626506024, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1787455, Length=134, Percent_Identity=36.5671641791045, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_NEOSM (Q2GE99)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506197.1
- ProteinModelPortal:   Q2GE99
- SMR:   Q2GE99
- STRING:   Q2GE99
- GeneID:   3931915
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0307
- TIGR:   NSE_0307
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   VAFMNNS
- ProtClustDB:   PRK05426
- BioCyc:   NSEN222891:NSE_0307-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 22098; Mature: 21967

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFRTFVLCGLGNPGLRYAQTRHNLGFMLIDYVRYTFSFPEFLPKFSGLLSSGRVSSFL
CCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
LYLFKPVAFMNNSGRPLVQLVNFYKVELENVIVFHDDIDLDFAKVKIKRGGGSGGHNGLK
HHHHHHHHHHCCCCCHHHHHHHHHHHEECEEEEEECCCCCEEEEEEEECCCCCCCCCCHH
SLDFNLGRDYWRFRFGVGRGVGDPAEHVLSRFTALELERLNKLFGFIGTNLPLLLSDIAT
HCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RKEKFLNEYKCCSQ
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SEFRTFVLCGLGNPGLRYAQTRHNLGFMLIDYVRYTFSFPEFLPKFSGLLSSGRVSSFL
CCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
LYLFKPVAFMNNSGRPLVQLVNFYKVELENVIVFHDDIDLDFAKVKIKRGGGSGGHNGLK
HHHHHHHHHHCCCCCHHHHHHHHHHHEECEEEEEECCCCCEEEEEEEECCCCCCCCCCHH
SLDFNLGRDYWRFRFGVGRGVGDPAEHVLSRFTALELERLNKLFGFIGTNLPLLLSDIAT
HCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RKEKFLNEYKCCSQ
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA