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The map label for this gene is ispD

Identifier: 88608617

GI number: 88608617

Start: 147285

End: 147983

Strand: Direct

Name: ispD

Synonym: NSE_0178

Alternate gene names: 88608617

Gene position: 147285-147983 (Clockwise)

Preceding gene: 88607962

Following gene: 88608290

Centisome position: 17.15

GC content: 43.49

Gene sequence:

>699_bases
ATGGGTAAAATTGCCGGTCTAGTTGTTGCTGGAGGTGGTGGTTCGAGGATAACGAATTCGGTTCTCCCAAAGCAGTACTT
GCAGGTCCGCGGCAAGGCAATTTTGCAGTACACAGTTGAAGCCCTTTTTGCCCATCCGAAAATTGAGTGTGTACACCTTG
TTGTCAATTCAAAGTGTGAGGTGCACTATTTGCCAATTTTGCGCAACTTGAGTGGGTATGTAGTTTCATTGTCAGAAGCT
GGTAATACTCGAACGGATTCAGTTTTTTCTGGGCTAAAGGCGTTAGAGTGCTTAAATCCTAGTCACGTATTGATACAGGA
TGCAGCTAGGCCTTTTACGACCCCTAAGGTGATCAATGCAGTAATTAAGAGCTTATTGGAGGGTTGTGAGGGTGTTGTTC
CAGTTGTGCCGGTACAGGACACAATCATAAAAAGAGAACCTGAAGGTATAGTAACCGATGTCAATAGAGATGAGTTAAGA
ATAGTGCAGACGCCACAAGGGTTTGATTTTTGCAAGATTTTTGCAGCATATAAGGCGCACTTCATGTGTCCTTCTAAGAG
GTATACAGATGACGGAAGTCTAGCGCTTGCACACGGTATAAAGGTGGAATGTATCCCAGGTGACAGCAGTAATCTTAAGA
TTACCCATCCTTTTGATTTGAAGTTTGCTGATTTTCTCTTAGCACAGAGTCACCGATAA

Upstream 100 bases:

>100_bases
ATAATGTGGGGAAGACTTTTTCAATTGACCGTGAATATGTAGATAAACATCTGCAGACTATCATCAAGAAGCTGGATTTG
TCGAAGTTTATTCTGTAAGA

Downstream 100 bases:

>100_bases
AATAGGTTGCTTGTATTTCTCATACTGCTTGTACTTTTCGCTTCTTGCGTTTTATTTATCTATTCCATCTTGAAAATTTT
CAGAAAGTGACGGAAAATTC

Product: putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MGKIAGLVVAGGGGSRITNSVLPKQYLQVRGKAILQYTVEALFAHPKIECVHLVVNSKCEVHYLPILRNLSGYVVSLSEA
GNTRTDSVFSGLKALECLNPSHVLIQDAARPFTTPKVINAVIKSLLEGCEGVVPVVPVQDTIIKREPEGIVTDVNRDELR
IVQTPQGFDFCKIFAAYKAHFMCPSKRYTDDGSLALAHGIKVECIPGDSSNLKITHPFDLKFADFLLAQSHR

Sequences:

>Translated_232_residues
MGKIAGLVVAGGGGSRITNSVLPKQYLQVRGKAILQYTVEALFAHPKIECVHLVVNSKCEVHYLPILRNLSGYVVSLSEA
GNTRTDSVFSGLKALECLNPSHVLIQDAARPFTTPKVINAVIKSLLEGCEGVVPVVPVQDTIIKREPEGIVTDVNRDELR
IVQTPQGFDFCKIFAAYKAHFMCPSKRYTDDGSLALAHGIKVECIPGDSSNLKITHPFDLKFADFLLAQSHR
>Mature_231_residues
GKIAGLVVAGGGGSRITNSVLPKQYLQVRGKAILQYTVEALFAHPKIECVHLVVNSKCEVHYLPILRNLSGYVVSLSEAG
NTRTDSVFSGLKALECLNPSHVLIQDAARPFTTPKVINAVIKSLLEGCEGVVPVVPVQDTIIKREPEGIVTDVNRDELRI
VQTPQGFDFCKIFAAYKAHFMCPSKRYTDDGSLALAHGIKVECIPGDSSNLKITHPFDLKFADFLLAQSHR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=236, Percent_Identity=27.9661016949153, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=28.695652173913, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_NEOSM (Q2GEM3)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506073.1
- ProteinModelPortal:   Q2GEM3
- STRING:   Q2GEM3
- GeneID:   3932002
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0178
- TIGR:   NSE_0178
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   REQQDFW
- PhylomeDB:   Q2GEM3
- ProtClustDB:   CLSK2528037
- BioCyc:   NSEN222891:NSE_0178-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25311; Mature: 25179

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIAGLVVAGGGGSRITNSVLPKQYLQVRGKAILQYTVEALFAHPKIECVHLVVNSKCE
CCCEEEEEEECCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VHYLPILRNLSGYVVSLSEAGNTRTDSVFSGLKALECLNPSHVLIQDAARPFTTPKVINA
EEEHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHH
VIKSLLEGCEGVVPVVPVQDTIIKREPEGIVTDVNRDELRIVQTPQGFDFCKIFAAYKAH
HHHHHHHHCCCCEEEECCCHHHHEECCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHH
FMCPSKRYTDDGSLALAHGIKVECIPGDSSNLKITHPFDLKFADFLLAQSHR
EECCCCCCCCCCCEEEECCCEEEEECCCCCCEEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
GKIAGLVVAGGGGSRITNSVLPKQYLQVRGKAILQYTVEALFAHPKIECVHLVVNSKCE
CCEEEEEEECCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VHYLPILRNLSGYVVSLSEAGNTRTDSVFSGLKALECLNPSHVLIQDAARPFTTPKVINA
EEEHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHH
VIKSLLEGCEGVVPVVPVQDTIIKREPEGIVTDVNRDELRIVQTPQGFDFCKIFAAYKAH
HHHHHHHHCCCCEEEECCCHHHHEECCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHH
FMCPSKRYTDDGSLALAHGIKVECIPGDSSNLKITHPFDLKFADFLLAQSHR
EECCCCCCCCCCCEEEECCCEEEEECCCCCCEEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA