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The map label for this gene is hslU

Identifier: 88607962

GI number: 88607962

Start: 145864

End: 147282

Strand: Direct

Name: hslU

Synonym: NSE_0177

Alternate gene names: 88607962

Gene position: 145864-147282 (Clockwise)

Preceding gene: 88608110

Following gene: 88608617

Centisome position: 16.98

GC content: 41.79

Gene sequence:

>1419_bases
ATGGGTACAGAGAAAGTCTTTAAGTATTCAGCTGCGGTTGGGCAGGATGCCGAACATATCTTGGAGGATGCTTATTCTGC
TGCTCCAAAGGCGCTTGTAAGTTATCTAGACAGATTTGTTGTTGGGCAGAAGTTGGCCAAGAAAAAAATTGCGATTGCAA
TAAGAAACCGCTGGAGGAGAAATAATATCCCGAAGCCGCTTCATGATGAAATCATTCCGAAAAATATACTTATGATAGGA
CCAACTGGTGTTGGAAAAACGGAAATTGCTAGACGCGTGGCAAAACTCTCAGGGGCACCGTTTATTAAGGTCGAGGCAAC
AAAGTTTACCGAGGTCGGCTATGTCGGTCGGGATGTCGAATCGATAATTCGGGATCTTGTTGATAGTGCCGTTGCCCAGG
TCAAAGATCAAAAGAGGAAGCAGTTTTCAAAAGAAGCTGAAGAATCTGCCAAGGAGAAAATTCTTGATGCGCTGGTTGGT
AAAAAAAATACTGAGGACGACGATCTGGCTGATGGTGAGGATGAAAAACATGATGCAAGAGCTGTTTTTGAGAAAAAGCT
CGATGAAGGGAAATTAGATGATGCAGAAATTGAAATAAATGTTCGTGAGCTTCCTCAGAATGTTTTTCCTACGATGGATG
TCCCAGGTATGCCAGGTACTCAGATCGGTATGATGAACATCGGGGACATGATGAGTAAGGTCTTTGGTGCCAATAAGAAG
TTCAAAAGAAAGAGAGTTAAAGTCAAAGATGCTAGGAAAATCCTTGCGGATGCAGAAGTCGAGGATCTTTTCGATGAAGA
CGCAATAATCAAAGAAGCATTAGATCTGGTGACGAACAGGGGTATGGTTTTTATCGATGAGATAGATAAAATCTGTGCTC
GAACAGAGGTTAGAGGGGAAGTGAATAGAGAGGGTGTTCAGAGGGATTTGTTACCTCTTTTAGAGGGAACCACGGTAGTG
ACAAAATATGGTGTTGTAAAAACGGATCACATACTTTTTGTTGGTTCTGGAGCGTTTCACTTTGCTAAACCGTCAGACTT
GCTACCAGAATTACAGGGACGTTTGCCAATAAGGGTAGAACTTGACTCGCTTGATGTAGAAGATATGATCCGTATTCTTA
CTGAAACTGAGTCCAGCCTATTGAAGCAGTACTGTGCATTATTGGAGACAGAAGGTGTTTCTCTTGAATTCACAAAAGAT
GGTGTGCGTGCAATTGCTGAAGCTGCAATAACGGTCAATAATGAAGTTGAAAATATTGGTGCGAGGCGTCTCCATACAAT
AATGGAAACACTACTTGAGGAAATTAACTTCGAGGCCAACGATAATGTGGGGAAGACTTTTTCAATTGACCGTGAATATG
TAGATAAACATCTGCAGACTATCATCAAGAAGCTGGATTTGTCGAAGTTTATTCTGTAA

Upstream 100 bases:

>100_bases
TAGCTGCTAAGATCTGCGTTTTTACAAATGAAAATTTTACGGTTGAAGTCATCGATTGTGCTTCAAAAGAGTGTAGTTGA
AGTTAGTGAGAGTTTAAAAC

Downstream 100 bases:

>100_bases
GAATGGGTAAAATTGCCGGTCTAGTTGTTGCTGGAGGTGGTGGTTCGAGGATAACGAATTCGGTTCTCCCAAAGCAGTAC
TTGCAGGTCCGCGGCAAGGC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MGTEKVFKYSAAVGQDAEHILEDAYSAAPKALVSYLDRFVVGQKLAKKKIAIAIRNRWRRNNIPKPLHDEIIPKNILMIG
PTGVGKTEIARRVAKLSGAPFIKVEATKFTEVGYVGRDVESIIRDLVDSAVAQVKDQKRKQFSKEAEESAKEKILDALVG
KKNTEDDDLADGEDEKHDARAVFEKKLDEGKLDDAEIEINVRELPQNVFPTMDVPGMPGTQIGMMNIGDMMSKVFGANKK
FKRKRVKVKDARKILADAEVEDLFDEDAIIKEALDLVTNRGMVFIDEIDKICARTEVRGEVNREGVQRDLLPLLEGTTVV
TKYGVVKTDHILFVGSGAFHFAKPSDLLPELQGRLPIRVELDSLDVEDMIRILTETESSLLKQYCALLETEGVSLEFTKD
GVRAIAEAAITVNNEVENIGARRLHTIMETLLEEINFEANDNVGKTFSIDREYVDKHLQTIIKKLDLSKFIL

Sequences:

>Translated_472_residues
MGTEKVFKYSAAVGQDAEHILEDAYSAAPKALVSYLDRFVVGQKLAKKKIAIAIRNRWRRNNIPKPLHDEIIPKNILMIG
PTGVGKTEIARRVAKLSGAPFIKVEATKFTEVGYVGRDVESIIRDLVDSAVAQVKDQKRKQFSKEAEESAKEKILDALVG
KKNTEDDDLADGEDEKHDARAVFEKKLDEGKLDDAEIEINVRELPQNVFPTMDVPGMPGTQIGMMNIGDMMSKVFGANKK
FKRKRVKVKDARKILADAEVEDLFDEDAIIKEALDLVTNRGMVFIDEIDKICARTEVRGEVNREGVQRDLLPLLEGTTVV
TKYGVVKTDHILFVGSGAFHFAKPSDLLPELQGRLPIRVELDSLDVEDMIRILTETESSLLKQYCALLETEGVSLEFTKD
GVRAIAEAAITVNNEVENIGARRLHTIMETLLEEINFEANDNVGKTFSIDREYVDKHLQTIIKKLDLSKFIL
>Mature_471_residues
GTEKVFKYSAAVGQDAEHILEDAYSAAPKALVSYLDRFVVGQKLAKKKIAIAIRNRWRRNNIPKPLHDEIIPKNILMIGP
TGVGKTEIARRVAKLSGAPFIKVEATKFTEVGYVGRDVESIIRDLVDSAVAQVKDQKRKQFSKEAEESAKEKILDALVGK
KNTEDDDLADGEDEKHDARAVFEKKLDEGKLDDAEIEINVRELPQNVFPTMDVPGMPGTQIGMMNIGDMMSKVFGANKKF
KRKRVKVKDARKILADAEVEDLFDEDAIIKEALDLVTNRGMVFIDEIDKICARTEVRGEVNREGVQRDLLPLLEGTTVVT
KYGVVKTDHILFVGSGAFHFAKPSDLLPELQGRLPIRVELDSLDVEDMIRILTETESSLLKQYCALLETEGVSLEFTKDG
VRAIAEAAITVNNEVENIGARRLHTIMETLLEEINFEANDNVGKTFSIDREYVDKHLQTIIKKLDLSKFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=250, Percent_Identity=30.4, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790366, Length=447, Percent_Identity=53.9149888143177, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1786642, Length=112, Percent_Identity=41.9642857142857, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648289, Length=224, Percent_Identity=31.6964285714286, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648291, Length=224, Percent_Identity=31.6964285714286, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 52834; Mature: 52703

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTEKVFKYSAAVGQDAEHILEDAYSAAPKALVSYLDRFVVGQKLAKKKIAIAIRNRWRR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNIPKPLHDEIIPKNILMIGPTGVGKTEIARRVAKLSGAPFIKVEATKFTEVGYVGRDVE
CCCCCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCHH
SIIRDLVDSAVAQVKDQKRKQFSKEAEESAKEKILDALVGKKNTEDDDLADGEDEKHDAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
AVFEKKLDEGKLDDAEIEINVRELPQNVFPTMDVPGMPGTQIGMMNIGDMMSKVFGANKK
HHHHHHHCCCCCCCCEEEEEHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHH
FKRKRVKVKDARKILADAEVEDLFDEDAIIKEALDLVTNRGMVFIDEIDKICARTEVRGE
HHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCC
VNREGVQRDLLPLLEGTTVVTKYGVVKTDHILFVGSGAFHFAKPSDLLPELQGRLPIRVE
HHHHHHHHHHHHHHCCCEEEEECCCEEECCEEEEECCCCCCCCCHHHHHHHHCCCCEEEE
LDSLDVEDMIRILTETESSLLKQYCALLETEGVSLEFTKDGVRAIAEAAITVNNEVENIG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCHHHHHH
ARRLHTIMETLLEEINFEANDNVGKTFSIDREYVDKHLQTIIKKLDLSKFIL
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTEKVFKYSAAVGQDAEHILEDAYSAAPKALVSYLDRFVVGQKLAKKKIAIAIRNRWRR
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNIPKPLHDEIIPKNILMIGPTGVGKTEIARRVAKLSGAPFIKVEATKFTEVGYVGRDVE
CCCCCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCHH
SIIRDLVDSAVAQVKDQKRKQFSKEAEESAKEKILDALVGKKNTEDDDLADGEDEKHDAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
AVFEKKLDEGKLDDAEIEINVRELPQNVFPTMDVPGMPGTQIGMMNIGDMMSKVFGANKK
HHHHHHHCCCCCCCCEEEEEHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHH
FKRKRVKVKDARKILADAEVEDLFDEDAIIKEALDLVTNRGMVFIDEIDKICARTEVRGE
HHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCC
VNREGVQRDLLPLLEGTTVVTKYGVVKTDHILFVGSGAFHFAKPSDLLPELQGRLPIRVE
HHHHHHHHHHHHHHCCCEEEEECCCEEECCEEEEECCCCCCCCCHHHHHHHHCCCCEEEE
LDSLDVEDMIRILTETESSLLKQYCALLETEGVSLEFTKDGVRAIAEAAITVNNEVENIG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCHHHHHH
ARRLHTIMETLLEEINFEANDNVGKTFSIDREYVDKHLQTIIKKLDLSKFIL
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA