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The map label for this gene is tmk

Identifier: 88608604

GI number: 88608604

Start: 476521

End: 477120

Strand: Reverse

Name: tmk

Synonym: NSE_0547

Alternate gene names: 88608604

Gene position: 477120-476521 (Counterclockwise)

Preceding gene: 88608348

Following gene: 88608723

Centisome position: 55.54

GC content: 40.5

Gene sequence:

>600_bases
GTGTTTATAGTATTAGAGGGAATTGATGGGTCCGGTAAGAGTACACAGGTAAAACTTTTGAGTAAGTTTCTTGCAAAAGA
TGGTTATCCTTGCGTTTTAACGAGGGAGCCCGGTGGTACTTCCTTTGCCGAGAGTTTAAGATCCATGATCCTCCATGATC
CTATAGATCCTATGGCGCGGTTGTTGCTCATAGTTAGTGCACGTGTTGATCATTACAATAAAGTGATACTGCCCGCATTG
AAAGAAGGTAAGGTTGTCGTTTGCGACAGGTTCATTTATTCGACCTTAGCATATCAGGGATATGGGGATAAAATTGACCT
ACAGACAATTTTAGATTTACACAGGCTTTCCGGTTGTCTGGTTGAACCTGATTTAACTCTGCTTCTTTTGGGATCTGGTC
ATAAAAAATTAGGTCGTGATAACTTTGAACGTATGCCCAGAGAGTACCTTTCGAACGTATGTATGGGATACGAGAAAATT
GCACGTATGTATCCGAATATTCATGTTATTAAATGCATGGGAGTCGGTCGTACGAGTGAGGAGATCGTTAAGATAGTACA
AGGAAAAATAAGTGAAAAACAAACTTCATCGTGTAGGTAG

Upstream 100 bases:

>100_bases
AGCTGTACTAAGGGTTAGAGTATGATATACAGAGATGCTATTTTCTAGATGCGGGTTCGCAGAAAGATTTGTTTTTCTTA
TGTAGTTTATACAGGGTAGT

Downstream 100 bases:

>100_bases
ATGCACTGCTTCTTCAAGTCGGTGGCTTTATAGACACGTTAATGATCCTTTCGTGAAAAAAGCGAAAGCTGAACAATACA
GATCCAGAGCGGCATACAAA

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MFIVLEGIDGSGKSTQVKLLSKFLAKDGYPCVLTREPGGTSFAESLRSMILHDPIDPMARLLLIVSARVDHYNKVILPAL
KEGKVVVCDRFIYSTLAYQGYGDKIDLQTILDLHRLSGCLVEPDLTLLLLGSGHKKLGRDNFERMPREYLSNVCMGYEKI
ARMYPNIHVIKCMGVGRTSEEIVKIVQGKISEKQTSSCR

Sequences:

>Translated_199_residues
MFIVLEGIDGSGKSTQVKLLSKFLAKDGYPCVLTREPGGTSFAESLRSMILHDPIDPMARLLLIVSARVDHYNKVILPAL
KEGKVVVCDRFIYSTLAYQGYGDKIDLQTILDLHRLSGCLVEPDLTLLLLGSGHKKLGRDNFERMPREYLSNVCMGYEKI
ARMYPNIHVIKCMGVGRTSEEIVKIVQGKISEKQTSSCR
>Mature_199_residues
MFIVLEGIDGSGKSTQVKLLSKFLAKDGYPCVLTREPGGTSFAESLRSMILHDPIDPMARLLLIVSARVDHYNKVILPAL
KEGKVVVCDRFIYSTLAYQGYGDKIDLQTILDLHRLSGCLVEPDLTLLLLGSGHKKLGRDNFERMPREYLSNVCMGYEKI
ARMYPNIHVIKCMGVGRTSEEIVKIVQGKISEKQTSSCR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=185, Percent_Identity=34.5945945945946, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_NEOSM (Q2GDL6)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506430.1
- HSSP:   P65248
- STRING:   Q2GDL6
- GeneID:   3931989
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0547
- TIGR:   NSE_0547
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   ERMPREY
- PhylomeDB:   Q2GDL6
- ProtClustDB:   CLSK2528025
- BioCyc:   NSEN222891:NSE_0547-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 22235; Mature: 22235

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIVLEGIDGSGKSTQVKLLSKFLAKDGYPCVLTREPGGTSFAESLRSMILHDPIDPMAR
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHH
LLLIVSARVDHYNKVILPALKEGKVVVCDRFIYSTLAYQGYGDKIDLQTILDLHRLSGCL
HHHHHHHHHHHCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCEE
VEPDLTLLLLGSGHKKLGRDNFERMPREYLSNVCMGYEKIARMYPNIHVIKCMGVGRTSE
ECCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHH
EIVKIVQGKISEKQTSSCR
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFIVLEGIDGSGKSTQVKLLSKFLAKDGYPCVLTREPGGTSFAESLRSMILHDPIDPMAR
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHH
LLLIVSARVDHYNKVILPALKEGKVVVCDRFIYSTLAYQGYGDKIDLQTILDLHRLSGCL
HHHHHHHHHHHCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCEE
VEPDLTLLLLGSGHKKLGRDNFERMPREYLSNVCMGYEKIARMYPNIHVIKCMGVGRTSE
ECCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHH
EIVKIVQGKISEKQTSSCR
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA