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The map label for this gene is sucB

Identifier: 88608348

GI number: 88608348

Start: 477331

End: 478614

Strand: Reverse

Name: sucB

Synonym: NSE_0548

Alternate gene names: 88608348

Gene position: 478614-477331 (Counterclockwise)

Preceding gene: 88608269

Following gene: 88608604

Centisome position: 55.72

GC content: 41.9

Gene sequence:

>1284_bases
ATGAAAAAAGTTCTCGTCCCAAGAATGGGTGAATCAATTGCAGAAGCATCCGTTGTGAAAATTATCAAAAATATTGGTGA
ATCCGTGAGGGAGGACGAATTACTTTTTGAGCTTGAAACCGATAAGGCAGCCGTAGAAGTATCTGCTCCTGTTTCTGGAA
TCCTGAGTAAAATCAACGTTGAAATAGGGCAAGCGGTAAAAGTAGACGATGTTCTCGGGTTAATTGATGAAAATGTTGTC
GCACCCGGTGGAGGTAACCCAATTTCATCTGGAGTTGGTGATCGTAATATCGTTCCGCCATCTGTTGCCATTGCAGGAGG
AGTCGCATTGGGTGCAAGCGCGGAAAAGAATATTTCCTCGATAAAATCTTCTGAGTTAATTTATGCAAAGCAGGATGCAC
CATCTGCGCGGATTCTGATGGAAGAAAAGTTCTTGTCCCCGTGTGATATCGTTGGTACGGGGAAAGATAACAGGATAAGG
AAAGTTGATGTATTAAGTCGACTGTTTTATGGTGATCCAGAGCAAGAAAAAGATTCGGAATCAGAACAGCGTGCAGTTGC
TGGTAGTAGTTCTGTGAGTCCTGGGTTCCCTGAAAGGGTTGTGCCGATGTCTAAGTTGAGACAAAGAATTGCGTCGAGAC
TGAAGGAATCACAAAATACAGCTGCAATTTTAACGACTTTCAATGAAGTAGATATGGGGAATGTCATTCAAATAAGAAAG
CGTTACAAGGATTCCTTTGAAAAGGTCCATGGCTTAAAACTTGGCTTTATGTCGTTTTTTGTACAAGCTGTTATTTGTGG
GCTTGAAGCTTTTCCGGAAATTAATGCCGAGATTCGTGGAAAGGACATAGTTTATAAGGATTACTATAATATTGGGGTTG
CCGTTGGAACAAAGAACGGTCTTGTTGTGCCAGTAATAAAAAATGCGCAGAATCTTTCCTTTGCAGAAGTTGAAAGACAA
ATACTTGAGTACGGAAAAAAGGCTAGGGATGGCAAGATAGAGCCAGACGATATGCAGGGTGGTACTTTTACCATTTCTAA
TGGTGGTATTTATGGTTCACTCATGTCAACGCCGATTATTAATCCCCCACAATCTGGCATACTTGGAATGCACGCAATAA
AAGAAAGGCCTATTGTTATTGATGGTGCGATTGTTGTGCGGCCAATGATGTACCTTGCTCTCTCTTATGATCACCGTATA
GTTGATGGTAGGGAGGCAGTGAGCTTCCTTGTTCGTGTGAAGGAGTGTTTAGAAAACCCTGAGAGACTCTTACTTAAAGT
CTAG

Upstream 100 bases:

>100_bases
AAGAAATGAAATTGCGGGAAGCTATGTCTATTCAGTTAGAGTGGGTTTATAATTGCGCCTGAGTGCTAGTGATAAACATT
TAATTTGTATTAACTGAAAC

Downstream 100 bases:

>100_bases
TAGAGCTTCTAGAGATGGTTCAAATTTTACCTGACCTTTTGCTTTGGGGGAATTTATTTACTTTGGCCGCGTGGTTGTGT
CTCTGTTATTCGGCAAGAGC

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKKVLVPRMGESIAEASVVKIIKNIGESVREDELLFELETDKAAVEVSAPVSGILSKINVEIGQAVKVDDVLGLIDENVV
APGGGNPISSGVGDRNIVPPSVAIAGGVALGASAEKNISSIKSSELIYAKQDAPSARILMEEKFLSPCDIVGTGKDNRIR
KVDVLSRLFYGDPEQEKDSESEQRAVAGSSSVSPGFPERVVPMSKLRQRIASRLKESQNTAAILTTFNEVDMGNVIQIRK
RYKDSFEKVHGLKLGFMSFFVQAVICGLEAFPEINAEIRGKDIVYKDYYNIGVAVGTKNGLVVPVIKNAQNLSFAEVERQ
ILEYGKKARDGKIEPDDMQGGTFTISNGGIYGSLMSTPIINPPQSGILGMHAIKERPIVIDGAIVVRPMMYLALSYDHRI
VDGREAVSFLVRVKECLENPERLLLKV

Sequences:

>Translated_427_residues
MKKVLVPRMGESIAEASVVKIIKNIGESVREDELLFELETDKAAVEVSAPVSGILSKINVEIGQAVKVDDVLGLIDENVV
APGGGNPISSGVGDRNIVPPSVAIAGGVALGASAEKNISSIKSSELIYAKQDAPSARILMEEKFLSPCDIVGTGKDNRIR
KVDVLSRLFYGDPEQEKDSESEQRAVAGSSSVSPGFPERVVPMSKLRQRIASRLKESQNTAAILTTFNEVDMGNVIQIRK
RYKDSFEKVHGLKLGFMSFFVQAVICGLEAFPEINAEIRGKDIVYKDYYNIGVAVGTKNGLVVPVIKNAQNLSFAEVERQ
ILEYGKKARDGKIEPDDMQGGTFTISNGGIYGSLMSTPIINPPQSGILGMHAIKERPIVIDGAIVVRPMMYLALSYDHRI
VDGREAVSFLVRVKECLENPERLLLKV
>Mature_427_residues
MKKVLVPRMGESIAEASVVKIIKNIGESVREDELLFELETDKAAVEVSAPVSGILSKINVEIGQAVKVDDVLGLIDENVV
APGGGNPISSGVGDRNIVPPSVAIAGGVALGASAEKNISSIKSSELIYAKQDAPSARILMEEKFLSPCDIVGTGKDNRIR
KVDVLSRLFYGDPEQEKDSESEQRAVAGSSSVSPGFPERVVPMSKLRQRIASRLKESQNTAAILTTFNEVDMGNVIQIRK
RYKDSFEKVHGLKLGFMSFFVQAVICGLEAFPEINAEIRGKDIVYKDYYNIGVAVGTKNGLVVPVIKNAQNLSFAEVERQ
ILEYGKKARDGKIEPDDMQGGTFTISNGGIYGSLMSTPIINPPQSGILGMHAIKERPIVIDGAIVVRPMMYLALSYDHRI
VDGREAVSFLVRVKECLENPERLLLKV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=58.6666666666667, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=30.1310043668122, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI203098816, Length=458, Percent_Identity=30.1310043668122, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI31711992, Length=296, Percent_Identity=32.0945945945946, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=26.605504587156, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=36.25, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786946, Length=428, Percent_Identity=42.9906542056075, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1786305, Length=449, Percent_Identity=28.0623608017817, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=227, Percent_Identity=49.7797356828194, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17560088, Length=446, Percent_Identity=29.5964125560538, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=28.2407407407407, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=423, Percent_Identity=43.4988179669031, Blast_Score=330, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=26.2222222222222, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=54.9356223175966, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=30.0699300699301, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=30.1310043668122, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=30.1310043668122, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46372; Mature: 46372

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLVPRMGESIAEASVVKIIKNIGESVREDELLFELETDKAAVEVSAPVSGILSKINV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCHHHHHHHHHH
EIGQAVKVDDVLGLIDENVVAPGGGNPISSGVGDRNIVPPSVAIAGGVALGASAEKNISS
HHCCEEEHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEECCCCHHHHHH
IKSSELIYAKQDAPSARILMEEKFLSPCDIVGTGKDNRIRKVDVLSRLFYGDPEQEKDSE
HCCCCEEEEECCCCCHHEEEHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCH
SEQRAVAGSSSVSPGFPERVVPMSKLRQRIASRLKESQNTAAILTTFNEVDMGNVIQIRK
HHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
RYKDSFEKVHGLKLGFMSFFVQAVICGLEAFPEINAEIRGKDIVYKDYYNIGVAVGTKNG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCEEEEEEECCCC
LVVPVIKNAQNLSFAEVERQILEYGKKARDGKIEPDDMQGGTFTISNGGIYGSLMSTPII
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHCCCCC
NPPQSGILGMHAIKERPIVIDGAIVVRPMMYLALSYDHRIVDGREAVSFLVRVKECLENP
CCCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
ERLLLKV
HHHEECC
>Mature Secondary Structure
MKKVLVPRMGESIAEASVVKIIKNIGESVREDELLFELETDKAAVEVSAPVSGILSKINV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCHHHHHHHHHH
EIGQAVKVDDVLGLIDENVVAPGGGNPISSGVGDRNIVPPSVAIAGGVALGASAEKNISS
HHCCEEEHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEECCCCHHHHHH
IKSSELIYAKQDAPSARILMEEKFLSPCDIVGTGKDNRIRKVDVLSRLFYGDPEQEKDSE
HCCCCEEEEECCCCCHHEEEHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCCH
SEQRAVAGSSSVSPGFPERVVPMSKLRQRIASRLKESQNTAAILTTFNEVDMGNVIQIRK
HHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHH
RYKDSFEKVHGLKLGFMSFFVQAVICGLEAFPEINAEIRGKDIVYKDYYNIGVAVGTKNG
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEECCEEEEEEECCCC
LVVPVIKNAQNLSFAEVERQILEYGKKARDGKIEPDDMQGGTFTISNGGIYGSLMSTPII
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHCCCCC
NPPQSGILGMHAIKERPIVIDGAIVVRPMMYLALSYDHRIVDGREAVSFLVRVKECLENP
CCCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
ERLLLKV
HHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]