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The map label for this gene is lpdA-1

Identifier: 88608597

GI number: 88608597

Start: 378409

End: 379782

Strand: Direct

Name: lpdA-1

Synonym: NSE_0463

Alternate gene names: 88608597

Gene position: 378409-379782 (Clockwise)

Preceding gene: 88608394

Following gene: 88608646

Centisome position: 44.05

GC content: 41.85

Gene sequence:

>1374_bases
ATGTACGATGTTATAGTAGTTGGTGGTGGTCCGGCCGGGTATCCTGCAGCGATTCGGGCGAGTAGGAGTGGTCTTAAGGT
TGCGCTTGTTGAAAAAAATAAGTTGGGAGGGGTGTGCCTCAATTGCGGGTGTATCCCAACTAAAGCTCTTTTGCACATTG
CAGAGAAGTACCATTTTGTAAAAACTGGAGCAGCTGAGCTAGGTATAAATGTTTCCAATGTTTTTCTTACCTTTAGTAGT
GCTATCGCGTATGCTCAAGAGAAAATTAAAAAACTTGCTGCTGGTGTTTCCTACCTTATGAAGAAAAATAAGGTAGAGCT
TTTTTATTCTAGTGGAAGAATTTTGCCTGGTAAGCAAGTCAAGTTAGAGGATTTAGGTAAGACTATAAGTGCAAAAAACA
TTATTCTCGCAACCGGGAGCACTCCAAAAGAGATTACGGGTCTGGAATATGACCATGAGCTGATTTGGAATTACAATGAT
GCAATGACGGCAACTAAAATGCCTAAATCGTTGTTGGTAGTTGGAGCTGGCGCGATAGGTGTTGAATTTGCGTGCATTTA
TAATGTTTTTGGTAGTAAGGTGACGGTCATAGAAATGCAGAACCAGATCCTTCCAGCTGAGGATACTGAAATCAGTAATC
TTGCCGAAGCTGCGTTTAAAGAAAGTGGAATCACTATACGGAAAGGTACTACTATTCAGTCCTTAAAGAAGGATAAAGAT
AAGGTTCTTGTAACATTAAGCGACGGAACTAACCTAGAGGTTGAAAGAATTTTGGTTGCAGGTGGCGTAGAGGCGAGCTC
TCAGAATCTTGGTCTTGAACAGATTCCTACGATAAGGATGAATAAGGGTTTTGTTTCTGTTGACAAGTACTGTGAAACAG
GCGAGCCTGGTGTATACGCAATTGGTGACTTAAGGGGCTTTCCTTGTGTCGCTCACAAGGCAATATATGATGCGTATGTT
TGTACGGCAAAGATAGCGGGAAAAGAACCTGTTCCTCTAGAAATGGATAGTATTCCTAGTTGTATTTATTCCTTTCCTTC
AATTGCAAGTGTTGGTTTGACAGAAGAGGCCGCTATAAGAATGGGTCATAAAGTGAAAATAGGCCGTGCAAAAGCTGAAG
GAAACGGAAAGTCAGTTGTACTTGGTAAGGATAAAGGTTTGGTCAAAACAGTCTTTGATGCCAAAACAGGGGAACTTCTG
GGAGCACACATAATTGGTTATGAAGCTACGGAGATTCTGAATGGTTACATTATTGCAAAAGCCTCGGAGGCAACTGTTGA
AAGCCTGAAGGCTGTTGTTTTTCCCCACCCAACTATTTCTGAGATGATGTATGAGGCAGTACTAGCAGCGGACAATGAGG
AAGTCCATTCGTAA

Upstream 100 bases:

>100_bases
GAGCTTGTACGGCTGACATAAAAAAGGCGAGTTGTGATTGCGTAAGTTGAATTCTGGATTGGCAGGGTAGTATGAAAGTT
TGCATTGGTTTTAGCTCTCT

Downstream 100 bases:

>100_bases
AGTACAACTTGCTATTTTCTGCGGTAGATATATCACTTCATTTATGCTGTCTTTTTAGAAAGTAGGTTCAGTATAAGTAA
ATGTTGCAGGGGTTATCTCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MYDVIVVGGGPAGYPAAIRASRSGLKVALVEKNKLGGVCLNCGCIPTKALLHIAEKYHFVKTGAAELGINVSNVFLTFSS
AIAYAQEKIKKLAAGVSYLMKKNKVELFYSSGRILPGKQVKLEDLGKTISAKNIILATGSTPKEITGLEYDHELIWNYND
AMTATKMPKSLLVVGAGAIGVEFACIYNVFGSKVTVIEMQNQILPAEDTEISNLAEAAFKESGITIRKGTTIQSLKKDKD
KVLVTLSDGTNLEVERILVAGGVEASSQNLGLEQIPTIRMNKGFVSVDKYCETGEPGVYAIGDLRGFPCVAHKAIYDAYV
CTAKIAGKEPVPLEMDSIPSCIYSFPSIASVGLTEEAAIRMGHKVKIGRAKAEGNGKSVVLGKDKGLVKTVFDAKTGELL
GAHIIGYEATEILNGYIIAKASEATVESLKAVVFPHPTISEMMYEAVLAADNEEVHS

Sequences:

>Translated_457_residues
MYDVIVVGGGPAGYPAAIRASRSGLKVALVEKNKLGGVCLNCGCIPTKALLHIAEKYHFVKTGAAELGINVSNVFLTFSS
AIAYAQEKIKKLAAGVSYLMKKNKVELFYSSGRILPGKQVKLEDLGKTISAKNIILATGSTPKEITGLEYDHELIWNYND
AMTATKMPKSLLVVGAGAIGVEFACIYNVFGSKVTVIEMQNQILPAEDTEISNLAEAAFKESGITIRKGTTIQSLKKDKD
KVLVTLSDGTNLEVERILVAGGVEASSQNLGLEQIPTIRMNKGFVSVDKYCETGEPGVYAIGDLRGFPCVAHKAIYDAYV
CTAKIAGKEPVPLEMDSIPSCIYSFPSIASVGLTEEAAIRMGHKVKIGRAKAEGNGKSVVLGKDKGLVKTVFDAKTGELL
GAHIIGYEATEILNGYIIAKASEATVESLKAVVFPHPTISEMMYEAVLAADNEEVHS
>Mature_457_residues
MYDVIVVGGGPAGYPAAIRASRSGLKVALVEKNKLGGVCLNCGCIPTKALLHIAEKYHFVKTGAAELGINVSNVFLTFSS
AIAYAQEKIKKLAAGVSYLMKKNKVELFYSSGRILPGKQVKLEDLGKTISAKNIILATGSTPKEITGLEYDHELIWNYND
AMTATKMPKSLLVVGAGAIGVEFACIYNVFGSKVTVIEMQNQILPAEDTEISNLAEAAFKESGITIRKGTTIQSLKKDKD
KVLVTLSDGTNLEVERILVAGGVEASSQNLGLEQIPTIRMNKGFVSVDKYCETGEPGVYAIGDLRGFPCVAHKAIYDAYV
CTAKIAGKEPVPLEMDSIPSCIYSFPSIASVGLTEEAAIRMGHKVKIGRAKAEGNGKSVVLGKDKGLVKTVFDAKTGELL
GAHIIGYEATEILNGYIIAKASEATVESLKAVVFPHPTISEMMYEAVLAADNEEVHS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=34.1304347826087, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=27.8260869565217, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI22035672, Length=451, Percent_Identity=27.0509977827051, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI291045266, Length=479, Percent_Identity=25.678496868476, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI148277065, Length=434, Percent_Identity=24.6543778801843, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI33519430, Length=434, Percent_Identity=24.6543778801843, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI33519428, Length=434, Percent_Identity=24.6543778801843, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI33519426, Length=434, Percent_Identity=24.6543778801843, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=24.6543778801843, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI291045268, Length=470, Percent_Identity=25.3191489361702, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786307, Length=445, Percent_Identity=31.4606741573034, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87082354, Length=460, Percent_Identity=28.0434782608696, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789915, Length=430, Percent_Identity=28.3720930232558, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI87081717, Length=449, Percent_Identity=24.4988864142539, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=33.6244541484716, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI71983419, Length=435, Percent_Identity=26.8965517241379, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=435, Percent_Identity=26.8965517241379, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=24.2553191489362, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=27.2349272349272, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=34.1052631578947, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=26.875, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=26.8398268398268, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=34.4226579520697, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=27.0212765957447, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=27.0212765957447, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24640551, Length=470, Percent_Identity=27.0212765957447, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=25.7322175732218, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48759; Mature: 48759

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDVIVVGGGPAGYPAAIRASRSGLKVALVEKNKLGGVCLNCGCIPTKALLHIAEKYHFV
CEEEEEECCCCCCCCHHHEECCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHEE
KTGAAELGINVSNVFLTFSSAIAYAQEKIKKLAAGVSYLMKKNKVELFYSSGRILPGKQV
ECCHHHHCCEEHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCE
KLEDLGKTISAKNIILATGSTPKEITGLEYDHELIWNYNDAMTATKMPKSLLVVGAGAIG
EHHHHCCEECCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCEEEEEECCHHH
VEFACIYNVFGSKVTVIEMQNQILPAEDTEISNLAEAAFKESGITIRKGTTIQSLKKDKD
HHHHEEHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHCCCC
KVLVTLSDGTNLEVERILVAGGVEASSQNLGLEQIPTIRMNKGFVSVDKYCETGEPGVYA
EEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHCCEEEECCCEEEHHHHCCCCCCCEEE
IGDLRGFPCVAHKAIYDAYVCTAKIAGKEPVPLEMDSIPSCIYSFPSIASVGLTEEAAIR
EECCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCHHHCCHHHHCCCCHHHCCCCHHHHHH
MGHKVKIGRAKAEGNGKSVVLGKDKGLVKTVFDAKTGELLGAHIIGYEATEILNGYIIAK
CCCEEEEEEEECCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEECCCHHHHHCCEEEEE
ASEATVESLKAVVFPHPTISEMMYEAVLAADNEEVHS
CCHHHHHHHHEEECCCCCHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MYDVIVVGGGPAGYPAAIRASRSGLKVALVEKNKLGGVCLNCGCIPTKALLHIAEKYHFV
CEEEEEECCCCCCCCHHHEECCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHEE
KTGAAELGINVSNVFLTFSSAIAYAQEKIKKLAAGVSYLMKKNKVELFYSSGRILPGKQV
ECCHHHHCCEEHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCE
KLEDLGKTISAKNIILATGSTPKEITGLEYDHELIWNYNDAMTATKMPKSLLVVGAGAIG
EHHHHCCEECCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCEEEEEECCHHH
VEFACIYNVFGSKVTVIEMQNQILPAEDTEISNLAEAAFKESGITIRKGTTIQSLKKDKD
HHHHEEHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHCCCC
KVLVTLSDGTNLEVERILVAGGVEASSQNLGLEQIPTIRMNKGFVSVDKYCETGEPGVYA
EEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHCCEEEECCCEEEHHHHCCCCCCCEEE
IGDLRGFPCVAHKAIYDAYVCTAKIAGKEPVPLEMDSIPSCIYSFPSIASVGLTEEAAIR
EECCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCHHHCCHHHHCCCCHHHCCCCHHHHHH
MGHKVKIGRAKAEGNGKSVVLGKDKGLVKTVFDAKTGELLGAHIIGYEATEILNGYIIAK
CCCEEEEEEEECCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEECCCHHHHHCCEEEEE
ASEATVESLKAVVFPHPTISEMMYEAVLAADNEEVHS
CCHHHHHHHHEEECCCCCHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]