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The map label for this gene is limC [H]

Identifier: 88608593

GI number: 88608593

Start: 363071

End: 363745

Strand: Direct

Name: limC [H]

Synonym: NSE_0446

Alternate gene names: 88608593

Gene position: 363071-363745 (Clockwise)

Preceding gene: 88608079

Following gene: 88607956

Centisome position: 42.27

GC content: 40.0

Gene sequence:

>675_bases
ATGCTGCAGGGAAAAATAGTCTTAGTAACTGGTGCGTGTAACAGGATTGGTGCATCTATAGCGCGAAGGTTCGCGTCGGA
AGGGGCTTCTCTAATCCTGCCTGTAGCAGATCTATCAGATCTTGATGAGCTAAATCAGGACATAAAAAAATTGGGAGGAG
AATCGATTCTGGTGCAAGCAGATATATTGGATGTTTCTCAGATGGAGTCGCTTAAAAATGCTATCTCAGGTACCTCAAAA
AAGTTAGATGTTCTTATCTCTACCCACTTCTATACTAGTGAGCCTAACCTCTTAGTGGATTATGAAGTTGATGAGTGGAG
AAGAATAGTAGAAGAAAATTTCTCCTTAAATTGGTATATGTTAAAGAACTTTGATGGGCTTTTGAAACGGTCTGAGCATG
GTAGAGTAGTTTTTCTTTCTCTTGAGAGAATTGTTGGAAAAAATCCTTTATACTTTTCCCCGTACGTTGCAAGCAGTGCA
GCCCTTGCTCAGCTTATGAAAGGATATGCTAATGATGTTCTAAATTATGGGATGTGTGTGAACATGGTTGCTCTTGAAGG
ATACGAGGAGGATATTTCTGTGGAACCTCAGCCCAAATGTGCGGAACTTTTCCTTAGTTTGAGCTCAGTACAGTGTAAGA
TTTCTGGCAGAAAGCATTATGTTTCTTACACTTAA

Upstream 100 bases:

>100_bases
TTAGAACAAGCATGGCCCCTGTACATATTGTAGTGTGTTTTACAAAGCCACCTTCCCTATTATAAGATCTTGTACAGGCT
TTTTTGTTGAGGAGACTTTT

Downstream 100 bases:

>100_bases
CCTTAAATACTACTTTACCTGAATTTAACTTTTACTTAGAGCCTATTTGCTTCTTAAAAAAGGTATATCGGTAGTGCAAG
TTTTTCCTGTGAATCGTGGA

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: (4R,6S)-carveol dehydrogenase; CDH; Carveol dehydrogenase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLQGKIVLVTGACNRIGASIARRFASEGASLILPVADLSDLDELNQDIKKLGGESILVQADILDVSQMESLKNAISGTSK
KLDVLISTHFYTSEPNLLVDYEVDEWRRIVEENFSLNWYMLKNFDGLLKRSEHGRVVFLSLERIVGKNPLYFSPYVASSA
ALAQLMKGYANDVLNYGMCVNMVALEGYEEDISVEPQPKCAELFLSLSSVQCKISGRKHYVSYT

Sequences:

>Translated_224_residues
MLQGKIVLVTGACNRIGASIARRFASEGASLILPVADLSDLDELNQDIKKLGGESILVQADILDVSQMESLKNAISGTSK
KLDVLISTHFYTSEPNLLVDYEVDEWRRIVEENFSLNWYMLKNFDGLLKRSEHGRVVFLSLERIVGKNPLYFSPYVASSA
ALAQLMKGYANDVLNYGMCVNMVALEGYEEDISVEPQPKCAELFLSLSSVQCKISGRKHYVSYT
>Mature_224_residues
MLQGKIVLVTGACNRIGASIARRFASEGASLILPVADLSDLDELNQDIKKLGGESILVQADILDVSQMESLKNAISGTSK
KLDVLISTHFYTSEPNLLVDYEVDEWRRIVEENFSLNWYMLKNFDGLLKRSEHGRVVFLSLERIVGKNPLYFSPYVASSA
ALAQLMKGYANDVLNYGMCVNMVALEGYEEDISVEPQPKCAELFLSLSSVQCKISGRKHYVSYT

Specific function: Catalyzes the oxidation of carveol to carvone, with a strong stereoselectivity since it efficiently converts only the (6S)-stereoisomers, of which (-)-(4R,6S)-trans-carveol is the better substrate. Displays a broad substrate specificity with a preference

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI1787335, Length=193, Percent_Identity=28.4974093264249, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 24927; Mature: 24927

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQGKIVLVTGACNRIGASIARRFASEGASLILPVADLSDLDELNQDIKKLGGESILVQA
CCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
DILDVSQMESLKNAISGTSKKLDVLISTHFYTSEPNLLVDYEVDEWRRIVEENFSLNWYM
CCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHCCCCEEEEE
LKNFDGLLKRSEHGRVVFLSLERIVGKNPLYFSPYVASSAALAQLMKGYANDVLNYGMCV
ECCCHHHHHCCCCCCEEEEEEHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
NMVALEGYEEDISVEPQPKCAELFLSLSSVQCKISGRKHYVSYT
HHHEECCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCEEEEECC
>Mature Secondary Structure
MLQGKIVLVTGACNRIGASIARRFASEGASLILPVADLSDLDELNQDIKKLGGESILVQA
CCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEE
DILDVSQMESLKNAISGTSKKLDVLISTHFYTSEPNLLVDYEVDEWRRIVEENFSLNWYM
CCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHCCCCEEEEE
LKNFDGLLKRSEHGRVVFLSLERIVGKNPLYFSPYVASSAALAQLMKGYANDVLNYGMCV
ECCCHHHHHCCCCCCEEEEEEHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
NMVALEGYEEDISVEPQPKCAELFLSLSSVQCKISGRKHYVSYT
HHHEECCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10473585 [H]