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The map label for this gene is dnaG

Identifier: 88608559

GI number: 88608559

Start: 847304

End: 849043

Strand: Reverse

Name: dnaG

Synonym: NSE_0961

Alternate gene names: 88608559

Gene position: 849043-847304 (Counterclockwise)

Preceding gene: 88608125

Following gene: 88608410

Centisome position: 98.84

GC content: 36.44

Gene sequence:

>1740_bases
GTGTTAAAAAAATACATAGAACTAATTAAAGAAAGGATTCCTGTTTCCGAGGTTGTAAGTAGGACGGTTAAACTCAAAAG
AGTTGGATCTAGACTTATTGGAAGATGTCCATTCCATCAAGAAAAGACGCCTTCGTTCTCGGTGAATGATAAAGGTAAAT
TCTTTTATTGTTTTGGTTGTGGTAAAAGTGGCGACGTTATAGATTTTGTTTCCCTAACAAAACGATTAGATCTGAGCGAA
GCTGTTGAAGAGTTAGCTTCCAGCATAGGCATAAAAATAGACAAGAAAAATTCTGGGACAGAAAGTTTTATCCAAGTATT
CCAATTTTTAGAAGAGTTAAGAGATTGGTTTTGTAGTAATTTGAACTCTAGAGTCGGTCAAGAAGCAAGTTACTACATAA
GAAACCGTCTAGATGATGATGTGATTTCTGAATTTAAGTTGGGTTATTGTCCTTCTAATTTGACCTCCTTCATCAACAAA
ACTAAGAATTTTCCTAGCACTGTGATTAAAAATTCTGGAGTCCTTGATAAACTGGGTAGATGTTACTTCAGAGATAGACT
GGTTTTTCCAATACACAATATACAGGGAAAAGTAATTGGATTTGGAAGTAGGGTTGTAAAGGAGGGTATTTTCCCAAAGT
ACATAAACTCTTGTGAAACGGAATTTTTCAAAAAGAGTGAACTGCTTTATAACATTGATAAGATTCCGAAGAATTCTGAA
GAACCCATTATATTAGTAGAGGGATATATGGATGTTATATCTCTCTGCCAATTTGGGTATAAAAGTGTTTTGGGTATCTT
AGGAACGTCTATAACGTCACATCATCTTAAACTACTCAACCGATTAAGGAGAAACCTAATATTTTGTTTTGATGGTGACA
ATGCTGGAATAAAAGCTGCTGGTAGAGTAGTGGAGCTCATCATGGATGAGTTCATAAATTCTCTAGAGATAAAAACTACT
TTCTCTTTCTTACCCATAGGTAAAGATCCCGATGATCTTGTGAGAAGTGATAGAGCTGCGTTTCAGCGTGTTTTAGATGG
TGCCAGAAGTTTATCTGATACAATATTCTTTTTAGCTAAATACGGGAGGCAATGCAGTTCGCCAGAGGACTTTATAGAGT
TGGAAAAGATCTTAGAAAAATATCTGATAAACGTGAAGGACTATAAAATTAGAAAACATTTCCAAGATTTTTTTCAGCAG
CAAATCTTTTTACTTAAAAGGAGTCTTCGTCAGCGTAATTTTATTCCTGCGTATAGGGGTGGCGAAGACGCCACGCATTT
TGTAGATGTTAAAAAACTTTCTTCCTCCGCCAGTATACACGTAGATAAGAAAGTCTTATTGCTGAAGATAGTTATAAAAT
TTCCAGAAATTCTGCGGAATGGTGATCGTGAAGAATACTTTTCTTTGTTAAAATTGAGCGATCCACGACATGTAAAACTC
CAATCCTCCATAGTGCAATTGCTCAACACCTCAATCGATATTAGTGGTGCATCTCTTGAAAGGGCATTGCTTGCAGACAG
CGAGACAGAAGCGTCTTATAAGCTTCTGAGTGATTTAGCTTCGCTTCCGGATGATATAGAAAAAGCAACCTTTTTATGGG
ATAGACTGCAGAAACAATTATGGCGCGACGAACTCCGAGAAGAGTATCGACGAATGATTCATTCATCTGATCTAGATGAG
AACAAAATGGAAGAAGCTCAAAGAATCTTGGTAAAGATTCGGGAGGCAGAAAAAGAATGA

Upstream 100 bases:

>100_bases
TCTCTTAGTGATGATGGGGACTATGCAATAGCCTTTGTTGTTCTTGGTGATAGAATATGAGGAACTTCCTCTTTTTTCTT
AGGTCGCGTGTTTTGTCTCT

Downstream 100 bases:

>100_bases
AGTTCATCTAAATTTGACTGCCATTTCTGTAGGGTATAAGTTTTGGTATTGCAGATCAGATCCTATTTAAAGTCTCTATG
AATATGTTTTTATTTCCCGG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLKKYIELIKERIPVSEVVSRTVKLKRVGSRLIGRCPFHQEKTPSFSVNDKGKFFYCFGCGKSGDVIDFVSLTKRLDLSE
AVEELASSIGIKIDKKNSGTESFIQVFQFLEELRDWFCSNLNSRVGQEASYYIRNRLDDDVISEFKLGYCPSNLTSFINK
TKNFPSTVIKNSGVLDKLGRCYFRDRLVFPIHNIQGKVIGFGSRVVKEGIFPKYINSCETEFFKKSELLYNIDKIPKNSE
EPIILVEGYMDVISLCQFGYKSVLGILGTSITSHHLKLLNRLRRNLIFCFDGDNAGIKAAGRVVELIMDEFINSLEIKTT
FSFLPIGKDPDDLVRSDRAAFQRVLDGARSLSDTIFFLAKYGRQCSSPEDFIELEKILEKYLINVKDYKIRKHFQDFFQQ
QIFLLKRSLRQRNFIPAYRGGEDATHFVDVKKLSSSASIHVDKKVLLLKIVIKFPEILRNGDREEYFSLLKLSDPRHVKL
QSSIVQLLNTSIDISGASLERALLADSETEASYKLLSDLASLPDDIEKATFLWDRLQKQLWRDELREEYRRMIHSSDLDE
NKMEEAQRILVKIREAEKE

Sequences:

>Translated_579_residues
MLKKYIELIKERIPVSEVVSRTVKLKRVGSRLIGRCPFHQEKTPSFSVNDKGKFFYCFGCGKSGDVIDFVSLTKRLDLSE
AVEELASSIGIKIDKKNSGTESFIQVFQFLEELRDWFCSNLNSRVGQEASYYIRNRLDDDVISEFKLGYCPSNLTSFINK
TKNFPSTVIKNSGVLDKLGRCYFRDRLVFPIHNIQGKVIGFGSRVVKEGIFPKYINSCETEFFKKSELLYNIDKIPKNSE
EPIILVEGYMDVISLCQFGYKSVLGILGTSITSHHLKLLNRLRRNLIFCFDGDNAGIKAAGRVVELIMDEFINSLEIKTT
FSFLPIGKDPDDLVRSDRAAFQRVLDGARSLSDTIFFLAKYGRQCSSPEDFIELEKILEKYLINVKDYKIRKHFQDFFQQ
QIFLLKRSLRQRNFIPAYRGGEDATHFVDVKKLSSSASIHVDKKVLLLKIVIKFPEILRNGDREEYFSLLKLSDPRHVKL
QSSIVQLLNTSIDISGASLERALLADSETEASYKLLSDLASLPDDIEKATFLWDRLQKQLWRDELREEYRRMIHSSDLDE
NKMEEAQRILVKIREAEKE
>Mature_579_residues
MLKKYIELIKERIPVSEVVSRTVKLKRVGSRLIGRCPFHQEKTPSFSVNDKGKFFYCFGCGKSGDVIDFVSLTKRLDLSE
AVEELASSIGIKIDKKNSGTESFIQVFQFLEELRDWFCSNLNSRVGQEASYYIRNRLDDDVISEFKLGYCPSNLTSFINK
TKNFPSTVIKNSGVLDKLGRCYFRDRLVFPIHNIQGKVIGFGSRVVKEGIFPKYINSCETEFFKKSELLYNIDKIPKNSE
EPIILVEGYMDVISLCQFGYKSVLGILGTSITSHHLKLLNRLRRNLIFCFDGDNAGIKAAGRVVELIMDEFINSLEIKTT
FSFLPIGKDPDDLVRSDRAAFQRVLDGARSLSDTIFFLAKYGRQCSSPEDFIELEKILEKYLINVKDYKIRKHFQDFFQQ
QIFLLKRSLRQRNFIPAYRGGEDATHFVDVKKLSSSASIHVDKKVLLLKIVIKFPEILRNGDREEYFSLLKLSDPRHVKL
QSSIVQLLNTSIDISGASLERALLADSETEASYKLLSDLASLPDDIEKATFLWDRLQKQLWRDELREEYRRMIHSSDLDE
NKMEEAQRILVKIREAEKE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=433, Percent_Identity=33.256351039261, Blast_Score=238, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66714; Mature: 66714

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKYIELIKERIPVSEVVSRTVKLKRVGSRLIGRCPFHQEKTPSFSVNDKGKFFYCFGC
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEC
GKSGDVIDFVSLTKRLDLSEAVEELASSIGIKIDKKNSGTESFIQVFQFLEELRDWFCSN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
LNSRVGQEASYYIRNRLDDDVISEFKLGYCPSNLTSFINKTKNFPSTVIKNSGVLDKLGR
HHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
CYFRDRLVFPIHNIQGKVIGFGSRVVKEGIFPKYINSCETEFFKKSELLYNIDKIPKNSE
HHHHCCEEEEEECCCCCEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPIILVEGYMDVISLCQFGYKSVLGILGTSITSHHLKLLNRLRRNLIFCFDGDNAGIKAA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHH
GRVVELIMDEFINSLEIKTTFSFLPIGKDPDDLVRSDRAAFQRVLDGARSLSDTIFFLAK
HHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGRQCSSPEDFIELEKILEKYLINVKDYKIRKHFQDFFQQQIFLLKRSLRQRNFIPAYRG
HCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GEDATHFVDVKKLSSSASIHVDKKVLLLKIVIKFPEILRNGDREEYFSLLKLSDPRHVKL
CCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
QSSIVQLLNTSIDISGASLERALLADSETEASYKLLSDLASLPDDIEKATFLWDRLQKQL
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
WRDELREEYRRMIHSSDLDENKMEEAQRILVKIREAEKE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKKYIELIKERIPVSEVVSRTVKLKRVGSRLIGRCPFHQEKTPSFSVNDKGKFFYCFGC
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEC
GKSGDVIDFVSLTKRLDLSEAVEELASSIGIKIDKKNSGTESFIQVFQFLEELRDWFCSN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHC
LNSRVGQEASYYIRNRLDDDVISEFKLGYCPSNLTSFINKTKNFPSTVIKNSGVLDKLGR
HHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
CYFRDRLVFPIHNIQGKVIGFGSRVVKEGIFPKYINSCETEFFKKSELLYNIDKIPKNSE
HHHHCCEEEEEECCCCCEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPIILVEGYMDVISLCQFGYKSVLGILGTSITSHHLKLLNRLRRNLIFCFDGDNAGIKAA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHH
GRVVELIMDEFINSLEIKTTFSFLPIGKDPDDLVRSDRAAFQRVLDGARSLSDTIFFLAK
HHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGRQCSSPEDFIELEKILEKYLINVKDYKIRKHFQDFFQQQIFLLKRSLRQRNFIPAYRG
HCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GEDATHFVDVKKLSSSASIHVDKKVLLLKIVIKFPEILRNGDREEYFSLLKLSDPRHVKL
CCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
QSSIVQLLNTSIDISGASLERALLADSETEASYKLLSDLASLPDDIEKATFLWDRLQKQL
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
WRDELREEYRRMIHSSDLDENKMEEAQRILVKIREAEKE
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA