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The map label for this gene is pdxJ

Identifier: 88608435

GI number: 88608435

Start: 264220

End: 264954

Strand: Direct

Name: pdxJ

Synonym: NSE_0320

Alternate gene names: 88608435

Gene position: 264220-264954 (Clockwise)

Preceding gene: 88608850

Following gene: 88608656

Centisome position: 30.76

GC content: 42.45

Gene sequence:

>735_bases
ATGACGAGAGTAAGTATTAATTTGGATAAGGTTGCGCTCCTTAGAAATTCAAGAAGTGCTGGAAGTCCCGATATCAAATA
TGTTGCGAATCTTGGCATTATGAGCGGTTGCAGCGGAATTACTTTGCATCCCAGGGCAGATAGGAGGCATGCTTTAATTG
AAGATGTTCAAATAATACTTGGCTTGAGCGAGGTTGCTTGTGGAGAAGTGGAATGCAATGTAGAGGGTGATCTAAGACCT
GAAATAATAGAACTTGTGGAGGCCAGTTCTATACATCAGTTTACGATTGTCCCGGTTAGAGATGGTGAAAGAACCACGGA
AAGAGGGTTTTCCGTTGGAGAGAAAGCAGAACTGCTTGGTTCGACTATAGAGAGGATCAAGAAAAAAAGAAATGTGCGCG
TAAGCCTTTTCGTAGAGCCAGAACCTGCATCTGTGAAGTATGCTGCCGAGGTTGGTTGTGATGCCGTTGAGCTTCATGCC
AAGTGGTATGCGCGCGCATTTTGTACTCATCGTGAATCTGAGGAAATCAAGAGACTGCATTTTGCTGCGCTAGAGGCGAG
AAATCTTGGACTGAAAGTAAATTTAGGTCATGATCTCAGTCTGGTAAATATTTCGACTGTAATAAATAAAATAAGACCAG
ATGAAATTTCTATAGGGCATGCCCTTATAGTTGAGTCATTCTTACAGGGCTTCTCAAGAACCTTAAGAAAGTATGTTTCT
ATCGCTAGTGGCTGA

Upstream 100 bases:

>100_bases
TGACAAAAATGATATGGTACATCTTCAGGTAGCTGAAGGGGTGATAGTCAAAATTTTAAAGAATTTTATTTCAGAGAAGA
GATAGGGGTATATTGGAATA

Downstream 100 bases:

>100_bases
AATAACATCTGCCATTTTGGCGATCTATATGGTATAAGACTATAGCTGTAGTAGTTTCTCTGAGCGTCGATGAGCAATTC
TTGTGATCTAACAGGCCACG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTRVSINLDKVALLRNSRSAGSPDIKYVANLGIMSGCSGITLHPRADRRHALIEDVQIILGLSEVACGEVECNVEGDLRP
EIIELVEASSIHQFTIVPVRDGERTTERGFSVGEKAELLGSTIERIKKKRNVRVSLFVEPEPASVKYAAEVGCDAVELHA
KWYARAFCTHRESEEIKRLHFAALEARNLGLKVNLGHDLSLVNISTVINKIRPDEISIGHALIVESFLQGFSRTLRKYVS
IASG

Sequences:

>Translated_244_residues
MTRVSINLDKVALLRNSRSAGSPDIKYVANLGIMSGCSGITLHPRADRRHALIEDVQIILGLSEVACGEVECNVEGDLRP
EIIELVEASSIHQFTIVPVRDGERTTERGFSVGEKAELLGSTIERIKKKRNVRVSLFVEPEPASVKYAAEVGCDAVELHA
KWYARAFCTHRESEEIKRLHFAALEARNLGLKVNLGHDLSLVNISTVINKIRPDEISIGHALIVESFLQGFSRTLRKYVS
IASG
>Mature_243_residues
TRVSINLDKVALLRNSRSAGSPDIKYVANLGIMSGCSGITLHPRADRRHALIEDVQIILGLSEVACGEVECNVEGDLRPE
IIELVEASSIHQFTIVPVRDGERTTERGFSVGEKAELLGSTIERIKKKRNVRVSLFVEPEPASVKYAAEVGCDAVELHAK
WYARAFCTHRESEEIKRLHFAALEARNLGLKVNLGHDLSLVNISTVINKIRPDEISIGHALIVESFLQGFSRTLRKYVSI
ASG

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=228, Percent_Identity=32.0175438596491, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26924; Mature: 26793

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVSINLDKVALLRNSRSAGSPDIKYVANLGIMSGCSGITLHPRADRRHALIEDVQIIL
CCEEEECHHHEEEEECCCCCCCCCHHHHHHCCHHCCCCCCEECCCCCCHHHHHHHHHHHH
GLSEVACGEVECNVEGDLRPEIIELVEASSIHQFTIVPVRDGERTTERGFSVGEKAELLG
HHHHHCCCCEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCCCHHHHHHH
STIERIKKKRNVRVSLFVEPEPASVKYAAEVGCDAVELHAKWYARAFCTHRESEEIKRLH
HHHHHHHHHCCCEEEEEECCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
FAALEARNLGLKVNLGHDLSLVNISTVINKIRPDEISIGHALIVESFLQGFSRTLRKYVS
HHHHHHHCCCEEEECCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IASG
HCCC
>Mature Secondary Structure 
TRVSINLDKVALLRNSRSAGSPDIKYVANLGIMSGCSGITLHPRADRRHALIEDVQIIL
CEEEECHHHEEEEECCCCCCCCCHHHHHHCCHHCCCCCCEECCCCCCHHHHHHHHHHHH
GLSEVACGEVECNVEGDLRPEIIELVEASSIHQFTIVPVRDGERTTERGFSVGEKAELLG
HHHHHCCCCEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCHHHHCCCCCHHHHHHH
STIERIKKKRNVRVSLFVEPEPASVKYAAEVGCDAVELHAKWYARAFCTHRESEEIKRLH
HHHHHHHHHCCCEEEEEECCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
FAALEARNLGLKVNLGHDLSLVNISTVINKIRPDEISIGHALIVESFLQGFSRTLRKYVS
HHHHHHHCCCEEEECCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IASG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA