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The map label for this gene is uvrD [H]

Identifier: 88608415

GI number: 88608415

Start: 290897

End: 292789

Strand: Reverse

Name: uvrD [H]

Synonym: NSE_0356

Alternate gene names: 88608415

Gene position: 292789-290897 (Counterclockwise)

Preceding gene: 88608135

Following gene: 88608644

Centisome position: 34.08

GC content: 40.46

Gene sequence:

>1893_bases
ATGTGGTACGATCTGGACCACCTTGCGCAATCAACAATTATGAACCTGAACGATGAGCAAAGACTTGCTGTGGAAACTGT
AAACGGTCCTGTCTTGATACTTGCTGGTGCGGGAACAGGTAAAACAAAAACGTTAGTGCATAGAATAGCCCACCTGATTA
GGTCAGGGTATGCGCATCCAAATCAAATTATGGCTGTGACCTTCGCAAATAAGGCTGCAAATGAAATGATTCAGCGTGTT
AATGAGATTGTAGGGGAAATGAAATTTCACTGGATTGGAACATTTCACGCAGCAGCAGCAAAGATCCTAAGAATTGAAGC
AGAGAAACTTGGCTTTAAACCGGACTTCACAATATTAGATGTAGACGACCAAGTCAGAATGCTTCAGAAAGTCGCTCAAG
AAAAAAAAATCGAAGCAGATAAAGCCCTACTTAAAAAGTACTTAAGCGTGATCTCTGCATGGAAAGATAAAGCCTTCACG
CCAAGTAGAGTAAGGTTCAATAATGCTGCAAATACATGGTTCGAGAAACAAGCACTCTCTGTGTATGATACTTACCAAAA
AAAACTCAAGGAGAACAATAGCATGGACTTCGGAGATTTGCTCATGCACAACGTTACTCTGTTTAACAAGGACGCAGAAA
CCTTGGAAAAATACAAAGAGAAATTTAGATACATCATGGTAGATGAGTACCAAGATACGAATTTATGTCAATACCTATGG
TTGAGGCTATTAGCGCAGAAGCACTCAAACATCTGCTGCGTTGGGGACGATGATCAATCAATCTATGGGTGGCGTGGTGC
AGAGGTAGGGAACATCTTGAGGTTTGCAAAAGATTTTGAAGGAGCAAAAGTCATCAAGCTGGAACAAAATTATCGCTCGA
CGAACAAAATACTCACTGCCGCACACAATATCATAAAAAATAATCAGTCCAGACTCACAAAGAAGCTGTGGACAGCATAC
CCTGGCGGCGAGGAAATACAAATTTTCACCTACATGGATAGTGAACAGGAGGCAAGAGCTATCATCAGAATGATAAAGGA
AAGAAAATTATTGTGTCGCTACAGCGATACGGCCATCCTTGTTAGGGCAGGCTTTCAGACGAGAGCCTTCGAGGAAGCAG
CTATTCACGAAAACGTCCCCTACCGCGTCGTTGGAGGACTTAAATTCTATGAGAGAAAAGAAATAAGAGACATTATTGCT
TACTTAAGGCTCGTTGTGAATCCAAATGACAATTTGGCCTTTGAAAGAATAATAAACACACCAAAAAGGGGCCTCGGACA
GGCATCACTGAACAAGATATATAACTACTCACTTGAAGAAGGGTGCTCGATGTTTGAAGCAGTCAGAAAACTTGAGGAAA
ACGAAAAACTTAAAAGCACAGCGCTTCTGACATTTGTAAAACAAGTTAGAAAATGGCAGGAAGAATCTCTCAATCACAGT
TTGCATGACTTGCTGAAAAAGATCACACTGGAATCCGGATATATATCAGCGTTAAAAGAGCAAGACGAGACAAGAATTGA
AAACGTTGATGAGCTTTTCAGAGTGATCCAAGAATTCGATGATGCTACTGATTTCCTACAACACGTATCACTTTTGACAG
AGAGTGACGCACAAATAAATAACAGTGATGCTGTCAACATGATGACACTGCACGCTTCTAAAGGACTAGAATTTGAGCAA
GTATTCCTCCCAGGTTGGGAGGATGGAACATTCCCACATGTAAGGTCTCTTTGTAGTACTGAACAAATTGAGGAGGAACG
TCGTTTAGCGTATGTTGGAATAACAAGAGCAAAAAGGTTTTTAACAATAACAAGTGCTCATAGAAGATTAATAAGAAATT
CTTGGCAAAATACTGTAAAATCGAGATTCATGTTGGAGTTAGCAGAGAAATAA

Upstream 100 bases:

>100_bases
ACGTACTCTTGACACATATCTCAGCGTAACAACACATTTGAAGAAACAACCCAGCAAATAAAGCGCAACAGCGCTTAAAC
AATATGTAGAAGAGAAAAAT

Downstream 100 bases:

>100_bases
AAATGAGATGGTCCTCAGCTGCAAATAAAAGGACCAATGCCCCAACTAAAAACAGACCATCAGCATTTAGCAAACATGAA
AAAGCGCACCATCATTTTAC

Product: UvrD/Rep family helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MWYDLDHLAQSTIMNLNDEQRLAVETVNGPVLILAGAGTGKTKTLVHRIAHLIRSGYAHPNQIMAVTFANKAANEMIQRV
NEIVGEMKFHWIGTFHAAAAKILRIEAEKLGFKPDFTILDVDDQVRMLQKVAQEKKIEADKALLKKYLSVISAWKDKAFT
PSRVRFNNAANTWFEKQALSVYDTYQKKLKENNSMDFGDLLMHNVTLFNKDAETLEKYKEKFRYIMVDEYQDTNLCQYLW
LRLLAQKHSNICCVGDDDQSIYGWRGAEVGNILRFAKDFEGAKVIKLEQNYRSTNKILTAAHNIIKNNQSRLTKKLWTAY
PGGEEIQIFTYMDSEQEARAIIRMIKERKLLCRYSDTAILVRAGFQTRAFEEAAIHENVPYRVVGGLKFYERKEIRDIIA
YLRLVVNPNDNLAFERIINTPKRGLGQASLNKIYNYSLEEGCSMFEAVRKLEENEKLKSTALLTFVKQVRKWQEESLNHS
LHDLLKKITLESGYISALKEQDETRIENVDELFRVIQEFDDATDFLQHVSLLTESDAQINNSDAVNMMTLHASKGLEFEQ
VFLPGWEDGTFPHVRSLCSTEQIEEERRLAYVGITRAKRFLTITSAHRRLIRNSWQNTVKSRFMLELAEK

Sequences:

>Translated_630_residues
MWYDLDHLAQSTIMNLNDEQRLAVETVNGPVLILAGAGTGKTKTLVHRIAHLIRSGYAHPNQIMAVTFANKAANEMIQRV
NEIVGEMKFHWIGTFHAAAAKILRIEAEKLGFKPDFTILDVDDQVRMLQKVAQEKKIEADKALLKKYLSVISAWKDKAFT
PSRVRFNNAANTWFEKQALSVYDTYQKKLKENNSMDFGDLLMHNVTLFNKDAETLEKYKEKFRYIMVDEYQDTNLCQYLW
LRLLAQKHSNICCVGDDDQSIYGWRGAEVGNILRFAKDFEGAKVIKLEQNYRSTNKILTAAHNIIKNNQSRLTKKLWTAY
PGGEEIQIFTYMDSEQEARAIIRMIKERKLLCRYSDTAILVRAGFQTRAFEEAAIHENVPYRVVGGLKFYERKEIRDIIA
YLRLVVNPNDNLAFERIINTPKRGLGQASLNKIYNYSLEEGCSMFEAVRKLEENEKLKSTALLTFVKQVRKWQEESLNHS
LHDLLKKITLESGYISALKEQDETRIENVDELFRVIQEFDDATDFLQHVSLLTESDAQINNSDAVNMMTLHASKGLEFEQ
VFLPGWEDGTFPHVRSLCSTEQIEEERRLAYVGITRAKRFLTITSAHRRLIRNSWQNTVKSRFMLELAEK
>Mature_630_residues
MWYDLDHLAQSTIMNLNDEQRLAVETVNGPVLILAGAGTGKTKTLVHRIAHLIRSGYAHPNQIMAVTFANKAANEMIQRV
NEIVGEMKFHWIGTFHAAAAKILRIEAEKLGFKPDFTILDVDDQVRMLQKVAQEKKIEADKALLKKYLSVISAWKDKAFT
PSRVRFNNAANTWFEKQALSVYDTYQKKLKENNSMDFGDLLMHNVTLFNKDAETLEKYKEKFRYIMVDEYQDTNLCQYLW
LRLLAQKHSNICCVGDDDQSIYGWRGAEVGNILRFAKDFEGAKVIKLEQNYRSTNKILTAAHNIIKNNQSRLTKKLWTAY
PGGEEIQIFTYMDSEQEARAIIRMIKERKLLCRYSDTAILVRAGFQTRAFEEAAIHENVPYRVVGGLKFYERKEIRDIIA
YLRLVVNPNDNLAFERIINTPKRGLGQASLNKIYNYSLEEGCSMFEAVRKLEENEKLKSTALLTFVKQVRKWQEESLNHS
LHDLLKKITLESGYISALKEQDETRIENVDELFRVIQEFDDATDFLQHVSLLTESDAQINNSDAVNMMTLHASKGLEFEQ
VFLPGWEDGTFPHVRSLCSTEQIEEERRLAYVGITRAKRFLTITSAHRRLIRNSWQNTVKSRFMLELAEK

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=635, Percent_Identity=40, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI48994965, Length=641, Percent_Identity=38.3775351014041, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787196, Length=356, Percent_Identity=26.9662921348315, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=546, Percent_Identity=29.8534798534799, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=684, Percent_Identity=23.2456140350877, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 72853; Mature: 72853

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYDLDHLAQSTIMNLNDEQRLAVETVNGPVLILAGAGTGKTKTLVHRIAHLIRSGYAHP
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
NQIMAVTFANKAANEMIQRVNEIVGEMKFHWIGTFHAAAAKILRIEAEKLGFKPDFTILD
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDDQVRMLQKVAQEKKIEADKALLKKYLSVISAWKDKAFTPSRVRFNNAANTWFEKQALS
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHH
VYDTYQKKLKENNSMDFGDLLMHNVTLFNKDAETLEKYKEKFRYIMVDEYQDTNLCQYLW
HHHHHHHHHHCCCCCCHHHHHHHHHHEECCCHHHHHHHHHHCCEEEEECCCCCCHHHHHH
LRLLAQKHSNICCVGDDDQSIYGWRGAEVGNILRFAKDFEGAKVIKLEQNYRSTNKILTA
HHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
AHNIIKNNQSRLTKKLWTAYPGGEEIQIFTYMDSEQEARAIIRMIKERKLLCRYSDTAIL
HHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHEEECCCEEE
VRAGFQTRAFEEAAIHENVPYRVVGGLKFYERKEIRDIIAYLRLVVNPNDNLAFERIINT
EECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
PKRGLGQASLNKIYNYSLEEGCSMFEAVRKLEENEKLKSTALLTFVKQVRKWQEESLNHS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LHDLLKKITLESGYISALKEQDETRIENVDELFRVIQEFDDATDFLQHVSLLTESDAQIN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NSDAVNMMTLHASKGLEFEQVFLPGWEDGTFPHVRSLCSTEQIEEERRLAYVGITRAKRF
CCCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHH
LTITSAHRRLIRNSWQNTVKSRFMLELAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWYDLDHLAQSTIMNLNDEQRLAVETVNGPVLILAGAGTGKTKTLVHRIAHLIRSGYAHP
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
NQIMAVTFANKAANEMIQRVNEIVGEMKFHWIGTFHAAAAKILRIEAEKLGFKPDFTILD
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDDQVRMLQKVAQEKKIEADKALLKKYLSVISAWKDKAFTPSRVRFNNAANTWFEKQALS
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHH
VYDTYQKKLKENNSMDFGDLLMHNVTLFNKDAETLEKYKEKFRYIMVDEYQDTNLCQYLW
HHHHHHHHHHCCCCCCHHHHHHHHHHEECCCHHHHHHHHHHCCEEEEECCCCCCHHHHHH
LRLLAQKHSNICCVGDDDQSIYGWRGAEVGNILRFAKDFEGAKVIKLEQNYRSTNKILTA
HHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
AHNIIKNNQSRLTKKLWTAYPGGEEIQIFTYMDSEQEARAIIRMIKERKLLCRYSDTAIL
HHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHEEECCCEEE
VRAGFQTRAFEEAAIHENVPYRVVGGLKFYERKEIRDIIAYLRLVVNPNDNLAFERIINT
EECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
PKRGLGQASLNKIYNYSLEEGCSMFEAVRKLEENEKLKSTALLTFVKQVRKWQEESLNHS
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LHDLLKKITLESGYISALKEQDETRIENVDELFRVIQEFDDATDFLQHVSLLTESDAQIN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NSDAVNMMTLHASKGLEFEQVFLPGWEDGTFPHVRSLCSTEQIEEERRLAYVGITRAKRF
CCCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHH
LTITSAHRRLIRNSWQNTVKSRFMLELAEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]