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The map label for this gene is aspS

Identifier: 88608344

GI number: 88608344

Start: 394279

End: 396045

Strand: Direct

Name: aspS

Synonym: NSE_0477

Alternate gene names: 88608344

Gene position: 394279-396045 (Clockwise)

Preceding gene: 88608080

Following gene: 88608117

Centisome position: 45.9

GC content: 41.03

Gene sequence:

>1767_bases
TTGCTGAAGTCAAGTTATAGAGATTGTGGAATTGGCGAAATATCAGAGGAGCACATTGGCAAAGTTATAAAGCTTGCCGG
ATGGGTTTTTAGAAAAAGGGATCATGGTGCAATTCTTTTCATTGACCTGAGAGATGCTTATGGTGTAACACAACTTGTCA
TATCAGAAAGTATCGATGGTTTTGAGGAGCTCAGATGTGTCTCCTGTGAGAGTGTGATCACGGTTCAGGGACTAGTTTCG
GCAAGATCACCTGAAACAATAAATAGTTCTCTTCAAAATGGTAGAATAGAGGTTATTGTTAAATCCTGGAGCGTGAATTC
TCATGCCTCTTCATTGCCACTTCAAGTGGATTCCGAGTTTCCTTATCCTGAGGAAACACGGTTGAGGTATAGGTATCTCG
ATCTTAGGAGGAATAAACTCAAGAAAAATATCATACTTCGTTCAAAGATTATTTCTGAGATACGGCGGTTTATGGAAGAC
GAGGGTTTTACAGAATTTCAGACGCCAATACTGACTGCTACCTCACCAGAAGGGGCTCGTGATTATATCGTTCCCAGTAG
GATTCATAAGGGAAAGTTTTACGCTTTGCCCCAGGCGCCACAACAATTCAAGCAGTTGCTTATGGTTGCTGGTTTTGATC
GATACTTTCAGATAGCACCATGCTTCCGTGATGAGGATTCTCGAGCAGATAGGTCTCCTGGGGAGTTTTATCAATTAGAT
GTAGAGATGTCCTTTGTGGGACAAGAGGAGGTATTTGAACTGATGGAACAGCTTTTAAAGCATATCTTCTCAAAGTACTC
AAATAGAAAAGTGTCTGGGAGTTTCGAGCGTATTCCATATAAGGAGTCAATGCTTCTATATGGGACAGATAAGCCTGATC
TTAGGAATCCAATAAAAATTGCTGATTTTACTTCTATCTTTAAGGAATCTGGCCTCGCAGTTTTCATAGAGCAAATACAA
AAAGGAGCTGTTGTTCGGGGGATTCCAGCTCCAAATTGCGGGAATAAGCCAAGAAAGTTTTTTGATGAGATGATAAGATA
TGCTACCACAGAGCTCTGTGCTCGTGGGTTAGCATACCTTTGTTTTGATAATGGGATTCCTAAGGGGCCGATTGCAAAAT
TTTTATCTTCTGAGCAAATAAGTGAGATTGCCAAAAAGGCTTCTCTTGGTAAGGGTGATGCAATTTTTTTCTCATGTGAT
GCTGAACGAGCAGCTACAAAAATTGCTGGGGCCATCAGAAAAAAACTAGGATTGGAACTTTCTCTGCTTGACAACTCAGA
GTACAAATTTTGTTGGATTGTTAACTTTCCGTATTTTGAATTGGATGAAACGACTGGGAAATTAGATTTTTCTCATAACC
CTTTTTCTATGCCTGAGGGTGGTTTGGAGGCTCTTAATGGAGATCCGCTGAAAGTTGTATCACAGCAGTACGATATTGTT
TGCAATGGGGTGGAACTATCGAGTGGTGCTGTAAGAAATCATAAGCCAGAGATAATGTATCGTGCTTTTGAGATACTTGG
CCTAGATAGGGAATATGTAAATCGTGTTTTTGGCGCTTTGATTGAGTCATTTCAGTATGGCGTACCGCCACATGCTGGGA
TAGCACCTGGAATAGACAGGATGGTGATGCTTATTGCAGAAGAGGAAAATATAAGGGAGGTAATTGCATTCCCAATGAAC
CAAAGATGTGAAGACACACTAATGAGAGCTCCTTCTCCTGTGGAGACAGAAAGATTACTTGAATGTGGGGTCAAGTTGGT
AGAGTAG

Upstream 100 bases:

>100_bases
GCTGTGTGTGGGTGGACAGAGTGGTGTGTTTTTGCACTCATGCAGGGATTTTCGTTACCGCAGTAGATCGATGATGAGGG
TCTTTCATATGGGGGTTGGA

Downstream 100 bases:

>100_bases
GGAACAGTAATACCGAGTGTTTGGGTCAAATGTGGAAGTATGAGGGTGTATTTATCCAGCAAGCTTGTCACACACCCTTC
CTATCTATTTGTCTAGTTTC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MLKSSYRDCGIGEISEEHIGKVIKLAGWVFRKRDHGAILFIDLRDAYGVTQLVISESIDGFEELRCVSCESVITVQGLVS
ARSPETINSSLQNGRIEVIVKSWSVNSHASSLPLQVDSEFPYPEETRLRYRYLDLRRNKLKKNIILRSKIISEIRRFMED
EGFTEFQTPILTATSPEGARDYIVPSRIHKGKFYALPQAPQQFKQLLMVAGFDRYFQIAPCFRDEDSRADRSPGEFYQLD
VEMSFVGQEEVFELMEQLLKHIFSKYSNRKVSGSFERIPYKESMLLYGTDKPDLRNPIKIADFTSIFKESGLAVFIEQIQ
KGAVVRGIPAPNCGNKPRKFFDEMIRYATTELCARGLAYLCFDNGIPKGPIAKFLSSEQISEIAKKASLGKGDAIFFSCD
AERAATKIAGAIRKKLGLELSLLDNSEYKFCWIVNFPYFELDETTGKLDFSHNPFSMPEGGLEALNGDPLKVVSQQYDIV
CNGVELSSGAVRNHKPEIMYRAFEILGLDREYVNRVFGALIESFQYGVPPHAGIAPGIDRMVMLIAEEENIREVIAFPMN
QRCEDTLMRAPSPVETERLLECGVKLVE

Sequences:

>Translated_588_residues
MLKSSYRDCGIGEISEEHIGKVIKLAGWVFRKRDHGAILFIDLRDAYGVTQLVISESIDGFEELRCVSCESVITVQGLVS
ARSPETINSSLQNGRIEVIVKSWSVNSHASSLPLQVDSEFPYPEETRLRYRYLDLRRNKLKKNIILRSKIISEIRRFMED
EGFTEFQTPILTATSPEGARDYIVPSRIHKGKFYALPQAPQQFKQLLMVAGFDRYFQIAPCFRDEDSRADRSPGEFYQLD
VEMSFVGQEEVFELMEQLLKHIFSKYSNRKVSGSFERIPYKESMLLYGTDKPDLRNPIKIADFTSIFKESGLAVFIEQIQ
KGAVVRGIPAPNCGNKPRKFFDEMIRYATTELCARGLAYLCFDNGIPKGPIAKFLSSEQISEIAKKASLGKGDAIFFSCD
AERAATKIAGAIRKKLGLELSLLDNSEYKFCWIVNFPYFELDETTGKLDFSHNPFSMPEGGLEALNGDPLKVVSQQYDIV
CNGVELSSGAVRNHKPEIMYRAFEILGLDREYVNRVFGALIESFQYGVPPHAGIAPGIDRMVMLIAEEENIREVIAFPMN
QRCEDTLMRAPSPVETERLLECGVKLVE
>Mature_588_residues
MLKSSYRDCGIGEISEEHIGKVIKLAGWVFRKRDHGAILFIDLRDAYGVTQLVISESIDGFEELRCVSCESVITVQGLVS
ARSPETINSSLQNGRIEVIVKSWSVNSHASSLPLQVDSEFPYPEETRLRYRYLDLRRNKLKKNIILRSKIISEIRRFMED
EGFTEFQTPILTATSPEGARDYIVPSRIHKGKFYALPQAPQQFKQLLMVAGFDRYFQIAPCFRDEDSRADRSPGEFYQLD
VEMSFVGQEEVFELMEQLLKHIFSKYSNRKVSGSFERIPYKESMLLYGTDKPDLRNPIKIADFTSIFKESGLAVFIEQIQ
KGAVVRGIPAPNCGNKPRKFFDEMIRYATTELCARGLAYLCFDNGIPKGPIAKFLSSEQISEIAKKASLGKGDAIFFSCD
AERAATKIAGAIRKKLGLELSLLDNSEYKFCWIVNFPYFELDETTGKLDFSHNPFSMPEGGLEALNGDPLKVVSQQYDIV
CNGVELSSGAVRNHKPEIMYRAFEILGLDREYVNRVFGALIESFQYGVPPHAGIAPGIDRMVMLIAEEENIREVIAFPMN
QRCEDTLMRAPSPVETERLLECGVKLVE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=588, Percent_Identity=34.6938775510204, Blast_Score=328, Evalue=9e-90,
Organism=Homo sapiens, GI226491502, Length=252, Percent_Identity=27.3809523809524, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI45439306, Length=295, Percent_Identity=26.7796610169492, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI5031815, Length=336, Percent_Identity=25.8928571428571, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI194272210, Length=336, Percent_Identity=25.8928571428571, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=41.6949152542373, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=27.9310344827586, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789256, Length=301, Percent_Identity=25.9136212624585, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787161, Length=263, Percent_Identity=25.4752851711027, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32566633, Length=599, Percent_Identity=33.7228714524207, Blast_Score=294, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=28.2142857142857, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994340, Length=236, Percent_Identity=27.1186440677966, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=236, Percent_Identity=27.1186440677966, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=236, Percent_Identity=27.1186440677966, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=307, Percent_Identity=24.4299674267101, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=640, Percent_Identity=31.5625, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323011, Length=261, Percent_Identity=29.5019157088123, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=250, Percent_Identity=24, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=266, Percent_Identity=27.8195488721804, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24584738, Length=603, Percent_Identity=34.3283582089552, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI17136276, Length=317, Percent_Identity=26.813880126183, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24640849, Length=234, Percent_Identity=26.0683760683761, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640851, Length=216, Percent_Identity=26.8518518518519, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66543; Mature: 66543

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSSYRDCGIGEISEEHIGKVIKLAGWVFRKRDHGAILFIDLRDAYGVTQLVISESIDG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHCCCC
FEELRCVSCESVITVQGLVSARSPETINSSLQNGRIEVIVKSWSVNSHASSLPLQVDSEF
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEECCCC
PYPEETRLRYRYLDLRRNKLKKNIILRSKIISEIRRFMEDEGFTEFQTPILTATSPEGAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCC
DYIVPSRIHKGKFYALPQAPQQFKQLLMVAGFDRYFQIAPCFRDEDSRADRSPGEFYQLD
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEE
VEMSFVGQEEVFELMEQLLKHIFSKYSNRKVSGSFERIPYKESMLLYGTDKPDLRNPIKI
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCH
ADFTSIFKESGLAVFIEQIQKGAVVRGIPAPNCGNKPRKFFDEMIRYATTELCARGLAYL
HHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
CFDNGIPKGPIAKFLSSEQISEIAKKASLGKGDAIFFSCDAERAATKIAGAIRKKLGLEL
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCEE
SLLDNSEYKFCWIVNFPYFELDETTGKLDFSHNPFSMPEGGLEALNGDPLKVVSQQYDIV
EEECCCCEEEEEEEECCCEEECCCCCCEECCCCCCCCCCCCHHHCCCCHHHHHCCCCCEE
CNGVELSSGAVRNHKPEIMYRAFEILGLDREYVNRVFGALIESFQYGVPPHAGIAPGIDR
ECCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MVMLIAEEENIREVIAFPMNQRCEDTLMRAPSPVETERLLECGVKLVE
EEEEEECCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCHHCCC
>Mature Secondary Structure
MLKSSYRDCGIGEISEEHIGKVIKLAGWVFRKRDHGAILFIDLRDAYGVTQLVISESIDG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHCCCC
FEELRCVSCESVITVQGLVSARSPETINSSLQNGRIEVIVKSWSVNSHASSLPLQVDSEF
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEECCCC
PYPEETRLRYRYLDLRRNKLKKNIILRSKIISEIRRFMEDEGFTEFQTPILTATSPEGAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCC
DYIVPSRIHKGKFYALPQAPQQFKQLLMVAGFDRYFQIAPCFRDEDSRADRSPGEFYQLD
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEE
VEMSFVGQEEVFELMEQLLKHIFSKYSNRKVSGSFERIPYKESMLLYGTDKPDLRNPIKI
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCH
ADFTSIFKESGLAVFIEQIQKGAVVRGIPAPNCGNKPRKFFDEMIRYATTELCARGLAYL
HHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
CFDNGIPKGPIAKFLSSEQISEIAKKASLGKGDAIFFSCDAERAATKIAGAIRKKLGLEL
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCEE
SLLDNSEYKFCWIVNFPYFELDETTGKLDFSHNPFSMPEGGLEALNGDPLKVVSQQYDIV
EEECCCCEEEEEEEECCCEEECCCCCCEECCCCCCCCCCCCHHHCCCCHHHHHCCCCCEE
CNGVELSSGAVRNHKPEIMYRAFEILGLDREYVNRVFGALIESFQYGVPPHAGIAPGIDR
ECCEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MVMLIAEEENIREVIAFPMNQRCEDTLMRAPSPVETERLLECGVKLVE
EEEEEECCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA