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The map label for this gene is pstA

Identifier: 88608117

GI number: 88608117

Start: 397522

End: 398715

Strand: Direct

Name: pstA

Synonym: NSE_0479

Alternate gene names: 88608117

Gene position: 397522-398715 (Clockwise)

Preceding gene: 88608344

Following gene: 88608225

Centisome position: 46.28

GC content: 41.04

Gene sequence:

>1194_bases
ATGAACATAAATTACCTTCGTTTAAAGCTTAACACGCGTAGTTTTATCAATAAGTTCTTCTCGTTTGTGTGCGTTTTTTG
CGCTTTTCTTCCGGTAGTTTTCCTTTTTGTACTGCTCTTTAGTACTTTTTCAGGAGCATATAAAGCTCTTCAGCAAACGA
AGTTTGCAGTTGACCTGGAGAAGGTAGGTATTGCATCTGGTGACTTACGTTACGAGATACGGGAAAAGCTCATAAGCTAT
TTGAAAGATCAGCTTCCATGGGAGGAAGATCCCTTTATTATAATGAATTTTTTTAGTAACTATGCGGTAGACGAAGTGTT
AGGCGCCTTGAACGTTGGAGAAAAAAAGGTTTGGGCGACAATTTCTGGCGAAGCAGATACAGTCCATAAATATCGACGCT
CCGGGAGTCCCGGAATGAAAAGGGTTGTCCAGTATTTTTCCGAGCGCGGACTTTTGAAAAAGGCGCTCAATTATAAGCTT
TTCACTAATCCTGATTCTAGAGATAGTAGCCAAGCTGGTATAGTAGGTGCGCTAATCGGATCTTTATATACTGTCTTTGT
TAGCTTGTTGGTTTCTTTTTTTATCGGTGTTCTGACGGCTTTGTGGCTTGGTGAATATAGCAGTAGGAGCAGCATCACGA
GAGTCCTTGAAGTGAGTATTAATAATCTTTCATCTACTCCACCGATTGTATTTGGTATACTTGGCCTAACGTTCTATATA
AACTTCCTTGGTTTGCCTAGGTCTTCTTCTCTGGTTGGAGGACTAACCTTAGCATTTATAATGTTTCCTATTCTAGTGAC
TTCTTCTTTACACGCGATCAGGAGTGTACCCGATAATATAAAGCAAGCCGCATTTGCTCTAGGTGCATCAAAGGTTCAGG
TTATCCTACACCATGTATTACCCCTTTCGATGCCAGGCATAATGACCGGAACTATCTTAGGGATTGCAAGGATTTTGGGA
GAGTCAGCACCTCTGCTGATGATAGGAATGGTTGCGTTTGTTGGTAGCATTCCGGAAAGCATCAGAGAGCCGGCAAATGT
TTTTGCGGTGCAGATCTATATATGGGCAACTAGTCCTGACATTTCAACGAATGAAAAGACTTCCATTATGGTACTTGTGC
TTTTGCTTTTACTTCTTGCGCTGAACTGCGTGGTTCACTTTATTAAAAAGGTATTTCACTACGATTTCCACTAA

Upstream 100 bases:

>100_bases
ATGTACTTCGAAAAGGTGACATCATCCGTTTTTTGTTGATTGAATTTGAGTTCATTCTATTTGTAAAGCTATAATGTGGG
TTCCGAAAGACCTGTCTGTA

Downstream 100 bases:

>100_bases
GATATTATGAGTTTGTCTCTGAAGGACGTTTCGCACTGTTATGAGCATTTTTCTATAGAGAAGGCTTGTTTTGAGTGTTC
TAGAGGGGAAATTGTTTGCT

Product: phosphate ABC transporter permease PstA

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNINYLRLKLNTRSFINKFFSFVCVFCAFLPVVFLFVLLFSTFSGAYKALQQTKFAVDLEKVGIASGDLRYEIREKLISY
LKDQLPWEEDPFIIMNFFSNYAVDEVLGALNVGEKKVWATISGEADTVHKYRRSGSPGMKRVVQYFSERGLLKKALNYKL
FTNPDSRDSSQAGIVGALIGSLYTVFVSLLVSFFIGVLTALWLGEYSSRSSITRVLEVSINNLSSTPPIVFGILGLTFYI
NFLGLPRSSSLVGGLTLAFIMFPILVTSSLHAIRSVPDNIKQAAFALGASKVQVILHHVLPLSMPGIMTGTILGIARILG
ESAPLLMIGMVAFVGSIPESIREPANVFAVQIYIWATSPDISTNEKTSIMVLVLLLLLLALNCVVHFIKKVFHYDFH

Sequences:

>Translated_397_residues
MNINYLRLKLNTRSFINKFFSFVCVFCAFLPVVFLFVLLFSTFSGAYKALQQTKFAVDLEKVGIASGDLRYEIREKLISY
LKDQLPWEEDPFIIMNFFSNYAVDEVLGALNVGEKKVWATISGEADTVHKYRRSGSPGMKRVVQYFSERGLLKKALNYKL
FTNPDSRDSSQAGIVGALIGSLYTVFVSLLVSFFIGVLTALWLGEYSSRSSITRVLEVSINNLSSTPPIVFGILGLTFYI
NFLGLPRSSSLVGGLTLAFIMFPILVTSSLHAIRSVPDNIKQAAFALGASKVQVILHHVLPLSMPGIMTGTILGIARILG
ESAPLLMIGMVAFVGSIPESIREPANVFAVQIYIWATSPDISTNEKTSIMVLVLLLLLLALNCVVHFIKKVFHYDFH
>Mature_397_residues
MNINYLRLKLNTRSFINKFFSFVCVFCAFLPVVFLFVLLFSTFSGAYKALQQTKFAVDLEKVGIASGDLRYEIREKLISY
LKDQLPWEEDPFIIMNFFSNYAVDEVLGALNVGEKKVWATISGEADTVHKYRRSGSPGMKRVVQYFSERGLLKKALNYKL
FTNPDSRDSSQAGIVGALIGSLYTVFVSLLVSFFIGVLTALWLGEYSSRSSITRVLEVSINNLSSTPPIVFGILGLTFYI
NFLGLPRSSSLVGGLTLAFIMFPILVTSSLHAIRSVPDNIKQAAFALGASKVQVILHHVLPLSMPGIMTGTILGIARILG
ESAPLLMIGMVAFVGSIPESIREPANVFAVQIYIWATSPDISTNEKTSIMVLVLLLLLLALNCVVHFIKKVFHYDFH

Specific function: Part of the binding-protein-dependent transport system yqgGHIJK. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=250, Percent_Identity=28.8, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 43995; Mature: 43995

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINYLRLKLNTRSFINKFFSFVCVFCAFLPVVFLFVLLFSTFSGAYKALQQTKFAVDLE
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVGIASGDLRYEIREKLISYLKDQLPWEEDPFIIMNFFSNYAVDEVLGALNVGEKKVWAT
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEE
ISGEADTVHKYRRSGSPGMKRVVQYFSERGLLKKALNYKLFTNPDSRDSSQAGIVGALIG
ECCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCEEECCCCCCCCCHHHHHHHHHH
SLYTVFVSLLVSFFIGVLTALWLGEYSSRSSITRVLEVSINNLSSTPPIVFGILGLTFYI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
NFLGLPRSSSLVGGLTLAFIMFPILVTSSLHAIRSVPDNIKQAAFALGASKVQVILHHVL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
PLSMPGIMTGTILGIARILGESAPLLMIGMVAFVGSIPESIREPANVFAVQIYIWATSPD
HHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCCHHEEEEEEEEEEECCC
ISTNEKTSIMVLVLLLLLLALNCVVHFIKKVFHYDFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNINYLRLKLNTRSFINKFFSFVCVFCAFLPVVFLFVLLFSTFSGAYKALQQTKFAVDLE
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVGIASGDLRYEIREKLISYLKDQLPWEEDPFIIMNFFSNYAVDEVLGALNVGEKKVWAT
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEE
ISGEADTVHKYRRSGSPGMKRVVQYFSERGLLKKALNYKLFTNPDSRDSSQAGIVGALIG
ECCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCEEECCCCCCCCCHHHHHHHHHH
SLYTVFVSLLVSFFIGVLTALWLGEYSSRSSITRVLEVSINNLSSTPPIVFGILGLTFYI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
NFLGLPRSSSLVGGLTLAFIMFPILVTSSLHAIRSVPDNIKQAAFALGASKVQVILHHVL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
PLSMPGIMTGTILGIARILGESAPLLMIGMVAFVGSIPESIREPANVFAVQIYIWATSPD
HHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCCHHEEEEEEEEEEECCC
ISTNEKTSIMVLVLLLLLLALNCVVHFIKKVFHYDFH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]