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The map label for this gene is dus [H]

Identifier: 88608299

GI number: 88608299

Start: 306962

End: 307957

Strand: Reverse

Name: dus [H]

Synonym: NSE_0373

Alternate gene names: 88608299

Gene position: 307957-306962 (Counterclockwise)

Preceding gene: 88607995

Following gene: 88608240

Centisome position: 35.85

GC content: 40.36

Gene sequence:

>996_bases
ATGAAACTCAACCTGGCCAATAACATAAGCATTGACAACGTAATTTTAGCACCCATGTCTGGGGTAACTGATATGCCTTA
CAGGGATCTTGTAAAAAGTTTTGGCGCGGGACTTGTGGTTTCCGAGATGATAGCAAGCAGAGCTATGATTCTGAAAACAA
AAGATACAATGAAAAAAGTTTCTTTGTCTCCTAGAGAAGATATCACTGCTATCCAATTAGCCGGGTTCGAGCCAGACGTA
ATGGCAGAAGCAGCAAAAATAAATGAAGATATGGGCGCTAAAATCATTGATATTAATTTCGGGTGCCCTGTTAAGAAAGT
AGTCAACGGATATGCTGGTTCCTACCTGATGAAAGACGAATGTCACGCAGCAAAAATCCTCGAAGCAACAGTAAAGGCAG
TTAAAATACCAGTAACGCTAAAAATGCGCACTGGTTGGGATGAAAATCACAGGAACGCTCCGAAATTAGCAAAAATTGCG
GAGGATCTTGGAATAAAGATGATCACTATACACGGACGAACACGAGCACAAATGTATAAAGGAAAGTCAGATTGGCAATT
TATCCGGACAGTAAAAAATACCGTTAAGATCCCAGTAATAGCAAACGGTGATATTAAAACGTACGAAGATGCTGAAAGAG
CCTTAGTAGAGTCTGGTGCTGACGGTATCATGATCGGTCGTGGAGCCTATGGAAAACCTTGGTCTATAAGAAATATGTTG
CACTTTCTCAGGACGGGCAAAAAATTACAAGAACCTACACCACAGGAAAAACTTCCGATAATACTGCAACATATTAGTTC
AATATTCTCTCACTATGGCACCGAAATGGGTATAGGAATCGCAAGAAAGCACATTCTCTGGTACAGCGCGAATTATAAAA
ACTCCGCTGAATTCCGACAACAAATATGCCAAGAAACATGCGTAGAAAAACTTCTCGAAAGAGTGGAAGAGTTCTTTCAT
TCAGCTTGTTGTACTACCAAACTACCACAACAATAA

Upstream 100 bases:

>100_bases
TTTGGTATAGAAGAGCGAGTTGAAATCCTAAAAGAGAATAACCTAGACGACTGTAAACAATTAACTAGTGACTTTGTGAA
TTTTATCATTCTAAAGAACA

Downstream 100 bases:

>100_bases
CTTCTTGTACCTCAAAAAAATATCTGTGACCCTAGTGAAGAAGGACAACTGTACTCAGCAGGCTCGCATCATCCCTTTTG
TTCATCACTAGATGAAGAGC

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKLNLANNISIDNVILAPMSGVTDMPYRDLVKSFGAGLVVSEMIASRAMILKTKDTMKKVSLSPREDITAIQLAGFEPDV
MAEAAKINEDMGAKIIDINFGCPVKKVVNGYAGSYLMKDECHAAKILEATVKAVKIPVTLKMRTGWDENHRNAPKLAKIA
EDLGIKMITIHGRTRAQMYKGKSDWQFIRTVKNTVKIPVIANGDIKTYEDAERALVESGADGIMIGRGAYGKPWSIRNML
HFLRTGKKLQEPTPQEKLPIILQHISSIFSHYGTEMGIGIARKHILWYSANYKNSAEFRQQICQETCVEKLLERVEEFFH
SACCTTKLPQQ

Sequences:

>Translated_331_residues
MKLNLANNISIDNVILAPMSGVTDMPYRDLVKSFGAGLVVSEMIASRAMILKTKDTMKKVSLSPREDITAIQLAGFEPDV
MAEAAKINEDMGAKIIDINFGCPVKKVVNGYAGSYLMKDECHAAKILEATVKAVKIPVTLKMRTGWDENHRNAPKLAKIA
EDLGIKMITIHGRTRAQMYKGKSDWQFIRTVKNTVKIPVIANGDIKTYEDAERALVESGADGIMIGRGAYGKPWSIRNML
HFLRTGKKLQEPTPQEKLPIILQHISSIFSHYGTEMGIGIARKHILWYSANYKNSAEFRQQICQETCVEKLLERVEEFFH
SACCTTKLPQQ
>Mature_331_residues
MKLNLANNISIDNVILAPMSGVTDMPYRDLVKSFGAGLVVSEMIASRAMILKTKDTMKKVSLSPREDITAIQLAGFEPDV
MAEAAKINEDMGAKIIDINFGCPVKKVVNGYAGSYLMKDECHAAKILEATVKAVKIPVTLKMRTGWDENHRNAPKLAKIA
EDLGIKMITIHGRTRAQMYKGKSDWQFIRTVKNTVKIPVIANGDIKTYEDAERALVESGADGIMIGRGAYGKPWSIRNML
HFLRTGKKLQEPTPQEKLPIILQHISSIFSHYGTEMGIGIARKHILWYSANYKNSAEFRQQICQETCVEKLLERVEEFFH
SACCTTKLPQQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=286, Percent_Identity=31.8181818181818, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI239788462, Length=286, Percent_Identity=31.8181818181818, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI40807366, Length=223, Percent_Identity=27.3542600896861, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=27.9279279279279, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI8923374, Length=280, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789660, Length=311, Percent_Identity=42.443729903537, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693211, Length=239, Percent_Identity=29.2887029288703, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=281, Percent_Identity=28.4697508896797, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=223, Percent_Identity=27.8026905829596, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=29.8165137614679, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=241, Percent_Identity=29.4605809128631, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=298, Percent_Identity=28.1879194630872, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=27.9661016949153, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585320, Length=295, Percent_Identity=30.5084745762712, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24580595, Length=221, Percent_Identity=30.316742081448, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19920448, Length=221, Percent_Identity=30.316742081448, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45549423, Length=238, Percent_Identity=27.7310924369748, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=27.6315789473684, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36988; Mature: 36988

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNLANNISIDNVILAPMSGVTDMPYRDLVKSFGAGLVVSEMIASRAMILKTKDTMKKV
CEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHEEECHHHHHHH
SLSPREDITAIQLAGFEPDVMAEAAKINEDMGAKIIDINFGCPVKKVVNGYAGSYLMKDE
CCCCCCCCCEEEEECCCHHHHHHHHHHCHHHCCEEEEEECCCCHHHHHCCCCCCEEEHHH
CHAAKILEATVKAVKIPVTLKMRTGWDENHRNAPKLAKIAEDLGIKMITIHGRTRAQMYK
HHHHHHHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHC
GKSDWQFIRTVKNTVKIPVIANGDIKTYEDAERALVESGADGIMIGRGAYGKPWSIRNML
CCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHH
HFLRTGKKLQEPTPQEKLPIILQHISSIFSHYGTEMGIGIARKHILWYSANYKNSAEFRQ
HHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCHHHHHHEEEEEECCCCCHHHHHH
QICQETCVEKLLERVEEFFHSACCTTKLPQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLNLANNISIDNVILAPMSGVTDMPYRDLVKSFGAGLVVSEMIASRAMILKTKDTMKKV
CEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHEEECHHHHHHH
SLSPREDITAIQLAGFEPDVMAEAAKINEDMGAKIIDINFGCPVKKVVNGYAGSYLMKDE
CCCCCCCCCEEEEECCCHHHHHHHHHHCHHHCCEEEEEECCCCHHHHHCCCCCCEEEHHH
CHAAKILEATVKAVKIPVTLKMRTGWDENHRNAPKLAKIAEDLGIKMITIHGRTRAQMYK
HHHHHHHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHC
GKSDWQFIRTVKNTVKIPVIANGDIKTYEDAERALVESGADGIMIGRGAYGKPWSIRNML
CCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHH
HFLRTGKKLQEPTPQEKLPIILQHISSIFSHYGTEMGIGIARKHILWYSANYKNSAEFRQ
HHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCHHHHHHEEEEEECCCCCHHHHHH
QICQETCVEKLLERVEEFFHSACCTTKLPQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]