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The map label for this gene is purC

Identifier: 88608255

GI number: 88608255

Start: 754295

End: 755017

Strand: Reverse

Name: purC

Synonym: NSE_0852

Alternate gene names: 88608255

Gene position: 755017-754295 (Counterclockwise)

Preceding gene: 88607965

Following gene: 88608333

Centisome position: 87.89

GC content: 39.28

Gene sequence:

>723_bases
ATGAGGGAAAGGCGAAAAATTTATGAGGGGAAAGCTAAGATACTATTTAGTTTTCCTGACAACCCTAACCTTGTAACGCA
GCATTTTAAGGATGATGTTACGGCTTATAATAATCGGAAACATTCTGTTATTCCAGGTAAGGGAGTAATCAATAACTATA
TATCCGCCTTCTTCATGCAAAACTTGCAAAATGTCGGAATTAAAACACATTTTGTGAAAGTGCTGAACATGAGAGAACAG
CTTGTAAAAAAGGCTGAATTAATACCCATTGAAGTCGTCATACGTAACATAGTTGCAGGTGGTCTTGCAAAAAGGCTCGG
TTTGGAAGAGGGCATGATTCTTGATGCTCCGCTGATAGAGACGTATTACAAAAGCGATTCTCTTGGAGACCCGATGGTCA
CCGATGATCATATTCTTTCATTTAATTGGCTCAAACTGTCTGAGATAGAGGAAATGAAAGTCATGGCATGGAGGATAAAT
GATTTTCTAAGTGGCGCGCTGTCATCTGCCGGTATCATACTTGTAGACTTGAAGCTAGAATTCGGTTTCTATGATGACCA
GATTATTCTGATTGATGAAATCAGTCCGGATACTTGTCGTTTTTGGGATACTGAAACAAAAGAAAAAATGGATAAGGACC
GCTTCAGAAGGGATTTAGGCGGAGTAAGTAAGTACTACAGGGAAGTCGCTAGACGTTTAGGTATTCTAAATGGTTCATTC
TGA

Upstream 100 bases:

>100_bases
TGGTAGTCAAAGTCAAAATAAACTTTCCCCACGTCGTTAAGTAGATTGCTTTGTGGTATAATTCTGATACCCTTTGGGGT
TTTTAGGTGTTGGGTTGACA

Downstream 100 bases:

>100_bases
GCGCTGTGTCAAGAAAGTTAGGCTCTATATAGCCCGCACGTGTGGGCCAGTGTTATAGGCTTATCGGATCTGGATTTTCT
GTTAATAGCTTTTTATTTAA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRERRKIYEGKAKILFSFPDNPNLVTQHFKDDVTAYNNRKHSVIPGKGVINNYISAFFMQNLQNVGIKTHFVKVLNMREQ
LVKKAELIPIEVVIRNIVAGGLAKRLGLEEGMILDAPLIETYYKSDSLGDPMVTDDHILSFNWLKLSEIEEMKVMAWRIN
DFLSGALSSAGIILVDLKLEFGFYDDQIILIDEISPDTCRFWDTETKEKMDKDRFRRDLGGVSKYYREVARRLGILNGSF

Sequences:

>Translated_240_residues
MRERRKIYEGKAKILFSFPDNPNLVTQHFKDDVTAYNNRKHSVIPGKGVINNYISAFFMQNLQNVGIKTHFVKVLNMREQ
LVKKAELIPIEVVIRNIVAGGLAKRLGLEEGMILDAPLIETYYKSDSLGDPMVTDDHILSFNWLKLSEIEEMKVMAWRIN
DFLSGALSSAGIILVDLKLEFGFYDDQIILIDEISPDTCRFWDTETKEKMDKDRFRRDLGGVSKYYREVARRLGILNGSF
>Mature_240_residues
MRERRKIYEGKAKILFSFPDNPNLVTQHFKDDVTAYNNRKHSVIPGKGVINNYISAFFMQNLQNVGIKTHFVKVLNMREQ
LVKKAELIPIEVVIRNIVAGGLAKRLGLEEGMILDAPLIETYYKSDSLGDPMVTDDHILSFNWLKLSEIEEMKVMAWRIN
DFLSGALSSAGIILVDLKLEFGFYDDQIILIDEISPDTCRFWDTETKEKMDKDRFRRDLGGVSKYYREVARRLGILNGSF

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=249, Percent_Identity=29.718875502008, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI5453539, Length=249, Percent_Identity=29.718875502008, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI119220559, Length=256, Percent_Identity=28.515625, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788820, Length=235, Percent_Identity=43.4042553191489, Blast_Score=213, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17531275, Length=219, Percent_Identity=31.5068493150685, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18860083, Length=234, Percent_Identity=30.7692307692308, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24583917, Length=244, Percent_Identity=29.9180327868852, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_NEOSM (Q2GCS7)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506719.1
- HSSP:   Q9X0X0
- ProteinModelPortal:   Q2GCS7
- STRING:   Q2GCS7
- GeneID:   3931615
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0852
- TIGR:   NSE_0852
- eggNOG:   COG0152
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- PhylomeDB:   Q2GCS7
- ProtClustDB:   PRK09362
- BioCyc:   NSEN222891:NSE_0852-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 27643; Mature: 27643

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERRKIYEGKAKILFSFPDNPNLVTQHFKDDVTAYNNRKHSVIPGKGVINNYISAFFMQ
CCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
NLQNVGIKTHFVKVLNMREQLVKKAELIPIEVVIRNIVAGGLAKRLGLEEGMILDAPLIE
HHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
TYYKSDSLGDPMVTDDHILSFNWLKLSEIEEMKVMAWRINDFLSGALSSAGIILVDLKLE
HHHCCCCCCCCCCCCCCEEEECCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
FGFYDDQIILIDEISPDTCRFWDTETKEKMDKDRFRRDLGGVSKYYREVARRLGILNGSF
ECCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRERRKIYEGKAKILFSFPDNPNLVTQHFKDDVTAYNNRKHSVIPGKGVINNYISAFFMQ
CCCHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
NLQNVGIKTHFVKVLNMREQLVKKAELIPIEVVIRNIVAGGLAKRLGLEEGMILDAPLIE
HHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
TYYKSDSLGDPMVTDDHILSFNWLKLSEIEEMKVMAWRINDFLSGALSSAGIILVDLKLE
HHHCCCCCCCCCCCCCCEEEECCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
FGFYDDQIILIDEISPDTCRFWDTETKEKMDKDRFRRDLGGVSKYYREVARRLGILNGSF
ECCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA