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The map label for this gene is hemF

Identifier: 88608200

GI number: 88608200

Start: 370372

End: 371292

Strand: Direct

Name: hemF

Synonym: NSE_0454

Alternate gene names: 88608200

Gene position: 370372-371292 (Clockwise)

Preceding gene: 88608089

Following gene: 88608050

Centisome position: 43.12

GC content: 41.37

Gene sequence:

>921_bases
ATGTACTCTCGAATTCTTTTGGGTTTGGGGGAACCAATGTATCATTACTGTTTGGGCAATATTCATAATTGTGAGGTCCG
TGTGTTAAGTGAGAAAAAAGAAACAGCAGCTGCCTGGTTTAGGGAGTTAAGAGATTGTATCAGGGATGAGTTTGTCGCTA
TAGAAAAAGAGTTTGATCACAATTTTCGGTCGGGTTTTGCAGTAGAATCTTGGGAACGAGAAGGTGGCGGAGGCGGTGAA
ATGAGCCTCATGTACGGTACTGTATTTGAGAAGATAGGTGTGAATATATCAACCGTGCATGGAGTTTTCAGCGAAGATTT
TGCATCTAAAATTCCTGGTGCACTAGAAAGTGGGGGGAATTTCTGGGCTAGTGGTATTTCATTGGTGGCGCATATGAGTT
CTCCACTTGTTCCTGCAGTGCATATGAATACACGCATGATAGTTACTGATAAATACTGGTTTGGTGGTGGTGCAGATTTG
ACTCCTATGTACTACAACGAGGAGGATGCAAAAACCTTCCACGCTGAGTTTAAAGAAACGTGTGATAGACATGATAGCAA
GTATTACAACGAATTCAGTAAGTGGGCGGATGAATATTTTTACGTAAAACATAGGAATGAACCTAGGGGAATAGGGGGAA
TATTCTACGATTACTTAAGAACAGATGATTGGGAGAAGGATTTTGCTTTCACAAAAGATGTTGGTAAGTGCTTTCTTTCT
GTATATCCAAAGATAGTGAGGAGAAATATGCATCTTTCTTATACAAATGAGCAAAGGCAGTTTCAGCTTCTCAAGAGGGG
CAGGTATGTTGAATTTAATCTTCTGTATGATAGAGGTACCGAGTTCGGGCTCAAGACTGGTGGTAACGTGAAGGCAATCC
TGATGTCTTTGCCCCCTGTGGTGGAGTGGAGAGATTTTTAA

Upstream 100 bases:

>100_bases
GATTCGGTAATGCCTCCAACGCTTAATTTGGATAAACCTGATGATTGTGCTAGTGGATTTAATTTAGTACCACACACATC
TCAAGAGAAGAATTTGCGGT

Downstream 100 bases:

>100_bases
AGGTACTTTGCTATCCTTTCTCGTCCAGTAGTTTGGTCTGATACATGGAGTTTGTTTGCCTGCTTGTTGTTCTAGCTGTT
TTTTTCTTTGTTTATCGGCA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MYSRILLGLGEPMYHYCLGNIHNCEVRVLSEKKETAAAWFRELRDCIRDEFVAIEKEFDHNFRSGFAVESWEREGGGGGE
MSLMYGTVFEKIGVNISTVHGVFSEDFASKIPGALESGGNFWASGISLVAHMSSPLVPAVHMNTRMIVTDKYWFGGGADL
TPMYYNEEDAKTFHAEFKETCDRHDSKYYNEFSKWADEYFYVKHRNEPRGIGGIFYDYLRTDDWEKDFAFTKDVGKCFLS
VYPKIVRRNMHLSYTNEQRQFQLLKRGRYVEFNLLYDRGTEFGLKTGGNVKAILMSLPPVVEWRDF

Sequences:

>Translated_306_residues
MYSRILLGLGEPMYHYCLGNIHNCEVRVLSEKKETAAAWFRELRDCIRDEFVAIEKEFDHNFRSGFAVESWEREGGGGGE
MSLMYGTVFEKIGVNISTVHGVFSEDFASKIPGALESGGNFWASGISLVAHMSSPLVPAVHMNTRMIVTDKYWFGGGADL
TPMYYNEEDAKTFHAEFKETCDRHDSKYYNEFSKWADEYFYVKHRNEPRGIGGIFYDYLRTDDWEKDFAFTKDVGKCFLS
VYPKIVRRNMHLSYTNEQRQFQLLKRGRYVEFNLLYDRGTEFGLKTGGNVKAILMSLPPVVEWRDF
>Mature_306_residues
MYSRILLGLGEPMYHYCLGNIHNCEVRVLSEKKETAAAWFRELRDCIRDEFVAIEKEFDHNFRSGFAVESWEREGGGGGE
MSLMYGTVFEKIGVNISTVHGVFSEDFASKIPGALESGGNFWASGISLVAHMSSPLVPAVHMNTRMIVTDKYWFGGGADL
TPMYYNEEDAKTFHAEFKETCDRHDSKYYNEFSKWADEYFYVKHRNEPRGIGGIFYDYLRTDDWEKDFAFTKDVGKCFLS
VYPKIVRRNMHLSYTNEQRQFQLLKRGRYVEFNLLYDRGTEFGLKTGGNVKAILMSLPPVVEWRDF

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III [H]

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI41393599, Length=254, Percent_Identity=44.8818897637795, Blast_Score=215, Evalue=3e-56,
Organism=Escherichia coli, GI1788777, Length=268, Percent_Identity=45.5223880597015, Blast_Score=227, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6320249, Length=290, Percent_Identity=41.0344827586207, Blast_Score=215, Evalue=6e-57,
Organism=Drosophila melanogaster, GI17864388, Length=271, Percent_Identity=46.1254612546125, Blast_Score=224, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001260
- InterPro:   IPR018375 [H]

Pfam domain/function: PF01218 Coprogen_oxidas [H]

EC number: =1.3.3.3 [H]

Molecular weight: Translated: 35438; Mature: 35438

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSRILLGLGEPMYHYCLGNIHNCEVRVLSEKKETAAAWFRELRDCIRDEFVAIEKEFDH
CCCCEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFRSGFAVESWEREGGGGGEMSLMYGTVFEKIGVNISTVHGVFSEDFASKIPGALESGGN
HHHCCCCEECHHCCCCCCCCEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCHHHCCCC
FWASGISLVAHMSSPLVPAVHMNTRMIVTDKYWFGGGADLTPMYYNEEDAKTFHAEFKET
HHHHHHHHHHHHCCCCCCEEECCCEEEEEECEEECCCCCCCCEEECCHHHHHHHHHHHHH
CDRHDSKYYNEFSKWADEYFYVKHRNEPRGIGGIFYDYLRTDDWEKDFAFTKDVGKCFLS
HHHCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VYPKIVRRNMHLSYTNEQRQFQLLKRGRYVEFNLLYDRGTEFGLKTGGNVKAILMSLPPV
HHHHHHHCCCCEEECCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCC
VEWRDF
CCCCCC
>Mature Secondary Structure
MYSRILLGLGEPMYHYCLGNIHNCEVRVLSEKKETAAAWFRELRDCIRDEFVAIEKEFDH
CCCCEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFRSGFAVESWEREGGGGGEMSLMYGTVFEKIGVNISTVHGVFSEDFASKIPGALESGGN
HHHCCCCEECHHCCCCCCCCEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCHHHCCCC
FWASGISLVAHMSSPLVPAVHMNTRMIVTDKYWFGGGADLTPMYYNEEDAKTFHAEFKET
HHHHHHHHHHHHCCCCCCEEECCCEEEEEECEEECCCCCCCCEEECCHHHHHHHHHHHHH
CDRHDSKYYNEFSKWADEYFYVKHRNEPRGIGGIFYDYLRTDDWEKDFAFTKDVGKCFLS
HHHCCHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VYPKIVRRNMHLSYTNEQRQFQLLKRGRYVEFNLLYDRGTEFGLKTGGNVKAILMSLPPV
HHHHHHHCCCCEEECCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCC
VEWRDF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA