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The map label for this gene is puuA [H]

Identifier: 88608196

GI number: 88608196

Start: 617009

End: 617827

Strand: Reverse

Name: puuA [H]

Synonym: NSE_0696

Alternate gene names: 88608196

Gene position: 617827-617009 (Counterclockwise)

Preceding gene: 88608388

Following gene: 88608747

Centisome position: 71.92

GC content: 41.76

Gene sequence:

>819_bases
ATGACTCTGGCAACTCTGAATGAACAGTTTAACTTTGGAATAAAATTAGGGATCGAATTGGAATTCTACAGTAGTGAAAA
AATCGGGTTTTTTAAGGAGTTGGTTTCGCAGCACTTTAGTTTGATCGAAGGTGTCTCTAAAGAAAGAGGGGATGGGCAGT
ATGAAATCTCGACACTTCCGACAGGTAACATATCTCTTCTTTTAAATGAGATAAACAGCTTTAAGAGCCTTGTACGTGGA
GTAGCAGATTTTTCGGCCAAACTACGCTTGGACCAGCCTGGAAGTGCCTTGCATGTTCATGTTAGTTTGCACGATAAAGG
GTCTCAAAAAAATATCACCAGGTTGGAACCTAAGCTAAGAAGCTTTATTGTTGGTGGATTTTGTCATTTAATGCGTTCTT
CTATGCTGTGTTTTGCTCCTAGTATTGAGTCCTATAGGCGCTTCCAATATTACGATAAGTTCACACCTAGGTTTATAAAT
TGGGGTTTTGAAGAGAACAAAAGTAATGCTGTACGGGTTACTTATGACACGATAGAGCATAGAGTAGCGGGGGCAGATGC
AGTACCCTCAAAAGTATTAGGACAAATAATGCGGGCGATCAAGTACGGCATTTGCAATGAGGTCGTTCCAAAGGCGCCAA
ATTTTGGAGAGAGTCAATATGCAGAGGCATTTCAGGATAGGCTGCCACTTTCATACTCTGAAGCTATAATTCATGGCGGT
GAGCACGGTTGTTTCGAGGGTATGATGTGTATGAATAGTGTTGGAATTCAGAACCATGCAGGCGAGGTTGGTGTGGGAAA
GTGTTTATGCTGCCATTAG

Upstream 100 bases:

>100_bases
CTTATATCAATTGAAGATAAGGTAATCAAGTTCACTGTGTTCTAGATGAACTTTCAGATTCGAAAGATATAGGTGATAAT
TGCGTGAAAATGTAACTAGT

Downstream 100 bases:

>100_bases
CTAAATTCCTCAGAATCGCTGCTGGATAAACTTGGTTTGTAAGGTGTGTTTTTCTTCAGAGTGTGACCGGTAGATGAAGT
AAAGCGTGCGGGACACAACA

Product: glutamine synthetase domain-containing protein

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTLATLNEQFNFGIKLGIELEFYSSEKIGFFKELVSQHFSLIEGVSKERGDGQYEISTLPTGNISLLLNEINSFKSLVRG
VADFSAKLRLDQPGSALHVHVSLHDKGSQKNITRLEPKLRSFIVGGFCHLMRSSMLCFAPSIESYRRFQYYDKFTPRFIN
WGFEENKSNAVRVTYDTIEHRVAGADAVPSKVLGQIMRAIKYGICNEVVPKAPNFGESQYAEAFQDRLPLSYSEAIIHGG
EHGCFEGMMCMNSVGIQNHAGEVGVGKCLCCH

Sequences:

>Translated_272_residues
MTLATLNEQFNFGIKLGIELEFYSSEKIGFFKELVSQHFSLIEGVSKERGDGQYEISTLPTGNISLLLNEINSFKSLVRG
VADFSAKLRLDQPGSALHVHVSLHDKGSQKNITRLEPKLRSFIVGGFCHLMRSSMLCFAPSIESYRRFQYYDKFTPRFIN
WGFEENKSNAVRVTYDTIEHRVAGADAVPSKVLGQIMRAIKYGICNEVVPKAPNFGESQYAEAFQDRLPLSYSEAIIHGG
EHGCFEGMMCMNSVGIQNHAGEVGVGKCLCCH
>Mature_271_residues
TLATLNEQFNFGIKLGIELEFYSSEKIGFFKELVSQHFSLIEGVSKERGDGQYEISTLPTGNISLLLNEINSFKSLVRGV
ADFSAKLRLDQPGSALHVHVSLHDKGSQKNITRLEPKLRSFIVGGFCHLMRSSMLCFAPSIESYRRFQYYDKFTPRFINW
GFEENKSNAVRVTYDTIEHRVAGADAVPSKVLGQIMRAIKYGICNEVVPKAPNFGESQYAEAFQDRLPLSYSEAIIHGGE
HGCFEGMMCMNSVGIQNHAGEVGVGKCLCCH

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=211, Percent_Identity=30.3317535545024, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 30377; Mature: 30246

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLATLNEQFNFGIKLGIELEFYSSEKIGFFKELVSQHFSLIEGVSKERGDGQYEISTLP
CCEEECCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
TGNISLLLNEINSFKSLVRGVADFSAKLRLDQPGSALHVHVSLHDKGSQKNITRLEPKLR
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHH
SFIVGGFCHLMRSSMLCFAPSIESYRRFQYYDKFTPRFINWGFEENKSNAVRVTYDTIEH
HHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEHHHHHH
RVAGADAVPSKVLGQIMRAIKYGICNEVVPKAPNFGESQYAEAFQDRLPLSYSEAIIHGG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCC
EHGCFEGMMCMNSVGIQNHAGEVGVGKCLCCH
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TLATLNEQFNFGIKLGIELEFYSSEKIGFFKELVSQHFSLIEGVSKERGDGQYEISTLP
CEEECCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
TGNISLLLNEINSFKSLVRGVADFSAKLRLDQPGSALHVHVSLHDKGSQKNITRLEPKLR
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHH
SFIVGGFCHLMRSSMLCFAPSIESYRRFQYYDKFTPRFINWGFEENKSNAVRVTYDTIEH
HHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEHHHHHH
RVAGADAVPSKVLGQIMRAIKYGICNEVVPKAPNFGESQYAEAFQDRLPLSYSEAIIHGG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCC
EHGCFEGMMCMNSVGIQNHAGEVGVGKCLCCH
CCCHHHHHHHHHHCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]