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The map label for this gene is recG

Identifier: 88608747

GI number: 88608747

Start: 609646

End: 611673

Strand: Reverse

Name: recG

Synonym: NSE_0690

Alternate gene names: 88608747

Gene position: 611673-609646 (Counterclockwise)

Preceding gene: 88608196

Following gene: 161511034

Centisome position: 71.21

GC content: 40.19

Gene sequence:

>2028_bases
ATGGCCAACTTGTTTGATCCAATAGATAAGATTTTTCTACTCGAAGAGAGAATGCTTGAGGTGGTACGTAACCTTGTTTC
TGGGAGTAGAGTTGTCGATCTGTTGCTGCATAAGCCAAGGGGATTTGTCAATAGGTGTAACTTTAAGGCGATTGCAAATT
CAGTCGAGGGAGAATTTGTAACGATCGAGGCAAGGTACGAGGGATCAAAAGAGCATTTTCATAGATCTAGACGAGTAAAG
CGACTTCATAAGTTTTTCTTCAAAGATAAAAATGAAGATTCTTTAGAAGTGATTTTTTTCACAAAAACTTACTTACAGAG
GAAGCTAAAAGTAGGAGAATGGTATCTCATAAACGGGAGAATGGGAGTGAGTGCTCAGATGTTTCATCCCGACAAGATAT
TAAAACTTAGTGAGCGAGATAAACTGTCGGCGTTTGAGCCAAAATATCGCCTTTCAGACGGTATAACTAACTACCAGTTT
TTATCTTTGGTTGACAGTTTACTTAAGTCGTCTAAACCTCCAAAAGAGTGGCTAGAGCAAACATCTGTAAGAAAGAGAGG
GCTAATATCATGGGGAAAAGCAATTGAGCAGTTACACCACCCATCATCCGAGGTTGAGTTCGAACAGGCATGCAAAAGGC
TTGCTTATGATGAGATTCTTGCAATGCATTTGGTGAACAACAGTCTGCATACTCAAGTCTTAAATCAGAAGAAGGAGCCT
ATAAAAGGAGATGGCAGAATTACAGCTCTTTTGCGTTCACGGTTGCCATTCAGTTTAACAGAAGGTCAAGAAGAGGTGAT
AAAAAAAATATATGCGCTGCAGGGGCAAGATGTAAGAATGGTAGCCCTACTACAAGGTGATGTTGGAAGTGGAAAGACAC
TTGTTGTGATTTTTGCCATGCTTAATGCAGTGGAGGTGGGTAAGCAAGTTGTTCTACTGTCACCAACCATCGTGTTAGCT
AAGCAACATTTTGAGGTTCTGCAAAAATTACTTCCGGAACTCAAACCTGTGCTTCTAACCGGGGAAAAGATTGCTTGTTC
TAGGGAGCAATTCTTACAAGACATTAGATCTGGGGATGCGAAAGTCATTGTTGCCACGCATGCAATTTTGGCTGTTGGGA
TACTTTTCTTTGATCTGGGGCTCTTGGTCATAGATGAGCAACATAGGTTTGGAGCAAATCAAAGGACGAAGTTGATCAGA
GAGAATCCTGGAGCTGACCTGGTGCTTGTAAGTGCTACCCCGATTCCCCGGACAATAGGACAAGTGTTGTTTGGTTCAAT
CACCTTGCTGAACCTTAGGGAAAAACCCAAATCTAGACCAGTCGTAACTACCTCTACAGTAACAAAAAGCAAAATTGCAA
AGCTCATTGAGAGGATTAAAGTGATTCTCGGAAAAGGGTCCAAAGTATTCTGGATTTGTCCCGTGATTGAGGAAAGCGAA
AATTTAAAAGTTATTTCTGTAACAGAAAGGTTTCGACTGCTTAAAAAACACTTTGCCGATGAAGTAGATTGTGTTCATGG
TGTGTTATCGGCGCAAAAAATGGAGGAAAAACTCACTGCTTTTCGCAATGGTAAAACGAAAATACTACTTGCTACAACCG
TTGTTGAAGTTGGTGTAGACATTCCTGATGTAGACGTAATAGTTATTGAAGATGCAGATCATTTTGGATTAGCTCAGCTC
CATCAACTTAGGGGTAGAGTAGGTCGCGGAAACAAAAAGTCTTTCTGCATTCTCCTTTATGATTCCCAGAAAATCACCGA
AAACGGAAAGAAGAGGCTAAAGATACTTAGAGAGTCCAGTGATGGTTTCTTCATAGCAGAAGAAGATTTTAAACTACGGG
GTGTTGGTGATATTTTCGGGGTCAGACAATCCGGATTTTACAAGTTTAAGTTTTTAGATAGACCTTTCGAACACTCATTT
TTTGATAGCGCATCAATGGAAGCACAGGATGCTATAAATATGGGCAGGATAGCTCAATATGAGGTGTTGATAAAAGTATT
TTTTTCAGATAATAATTATTACCTGTAG

Upstream 100 bases:

>100_bases
GCTTTGGATTGAGTGATACTCTCTCTTGCAGGAAGAGCGTTTCTTACATTTGATCTTGAATGCATATAATGTCTAAATCC
GTTTATCGGTGGCGAATCAC

Downstream 100 bases:

>100_bases
CTCGGCCCGAGTCCGCGCTGTGTCTTATTTTCGGAAAAGTTTTGTTTTCGGTCTAAATTATATTTGATGCCTACGATCAA
TCAGTTGGTTAGGAAACCCA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MANLFDPIDKIFLLEERMLEVVRNLVSGSRVVDLLLHKPRGFVNRCNFKAIANSVEGEFVTIEARYEGSKEHFHRSRRVK
RLHKFFFKDKNEDSLEVIFFTKTYLQRKLKVGEWYLINGRMGVSAQMFHPDKILKLSERDKLSAFEPKYRLSDGITNYQF
LSLVDSLLKSSKPPKEWLEQTSVRKRGLISWGKAIEQLHHPSSEVEFEQACKRLAYDEILAMHLVNNSLHTQVLNQKKEP
IKGDGRITALLRSRLPFSLTEGQEEVIKKIYALQGQDVRMVALLQGDVGSGKTLVVIFAMLNAVEVGKQVVLLSPTIVLA
KQHFEVLQKLLPELKPVLLTGEKIACSREQFLQDIRSGDAKVIVATHAILAVGILFFDLGLLVIDEQHRFGANQRTKLIR
ENPGADLVLVSATPIPRTIGQVLFGSITLLNLREKPKSRPVVTTSTVTKSKIAKLIERIKVILGKGSKVFWICPVIEESE
NLKVISVTERFRLLKKHFADEVDCVHGVLSAQKMEEKLTAFRNGKTKILLATTVVEVGVDIPDVDVIVIEDADHFGLAQL
HQLRGRVGRGNKKSFCILLYDSQKITENGKKRLKILRESSDGFFIAEEDFKLRGVGDIFGVRQSGFYKFKFLDRPFEHSF
FDSASMEAQDAINMGRIAQYEVLIKVFFSDNNYYL

Sequences:

>Translated_675_residues
MANLFDPIDKIFLLEERMLEVVRNLVSGSRVVDLLLHKPRGFVNRCNFKAIANSVEGEFVTIEARYEGSKEHFHRSRRVK
RLHKFFFKDKNEDSLEVIFFTKTYLQRKLKVGEWYLINGRMGVSAQMFHPDKILKLSERDKLSAFEPKYRLSDGITNYQF
LSLVDSLLKSSKPPKEWLEQTSVRKRGLISWGKAIEQLHHPSSEVEFEQACKRLAYDEILAMHLVNNSLHTQVLNQKKEP
IKGDGRITALLRSRLPFSLTEGQEEVIKKIYALQGQDVRMVALLQGDVGSGKTLVVIFAMLNAVEVGKQVVLLSPTIVLA
KQHFEVLQKLLPELKPVLLTGEKIACSREQFLQDIRSGDAKVIVATHAILAVGILFFDLGLLVIDEQHRFGANQRTKLIR
ENPGADLVLVSATPIPRTIGQVLFGSITLLNLREKPKSRPVVTTSTVTKSKIAKLIERIKVILGKGSKVFWICPVIEESE
NLKVISVTERFRLLKKHFADEVDCVHGVLSAQKMEEKLTAFRNGKTKILLATTVVEVGVDIPDVDVIVIEDADHFGLAQL
HQLRGRVGRGNKKSFCILLYDSQKITENGKKRLKILRESSDGFFIAEEDFKLRGVGDIFGVRQSGFYKFKFLDRPFEHSF
FDSASMEAQDAINMGRIAQYEVLIKVFFSDNNYYL
>Mature_674_residues
ANLFDPIDKIFLLEERMLEVVRNLVSGSRVVDLLLHKPRGFVNRCNFKAIANSVEGEFVTIEARYEGSKEHFHRSRRVKR
LHKFFFKDKNEDSLEVIFFTKTYLQRKLKVGEWYLINGRMGVSAQMFHPDKILKLSERDKLSAFEPKYRLSDGITNYQFL
SLVDSLLKSSKPPKEWLEQTSVRKRGLISWGKAIEQLHHPSSEVEFEQACKRLAYDEILAMHLVNNSLHTQVLNQKKEPI
KGDGRITALLRSRLPFSLTEGQEEVIKKIYALQGQDVRMVALLQGDVGSGKTLVVIFAMLNAVEVGKQVVLLSPTIVLAK
QHFEVLQKLLPELKPVLLTGEKIACSREQFLQDIRSGDAKVIVATHAILAVGILFFDLGLLVIDEQHRFGANQRTKLIRE
NPGADLVLVSATPIPRTIGQVLFGSITLLNLREKPKSRPVVTTSTVTKSKIAKLIERIKVILGKGSKVFWICPVIEESEN
LKVISVTERFRLLKKHFADEVDCVHGVLSAQKMEEKLTAFRNGKTKILLATTVVEVGVDIPDVDVIVIEDADHFGLAQLH
QLRGRVGRGNKKSFCILLYDSQKITENGKKRLKILRESSDGFFIAEEDFKLRGVGDIFGVRQSGFYKFKFLDRPFEHSFF
DSASMEAQDAINMGRIAQYEVLIKVFFSDNNYYL

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=631, Percent_Identity=32.4881141045959, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1787357, Length=386, Percent_Identity=34.1968911917098, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76712; Mature: 76580

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLFDPIDKIFLLEERMLEVVRNLVSGSRVVDLLLHKPRGFVNRCNFKAIANSVEGEFV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCHHHHHHCCCCCEE
TIEARYEGSKEHFHRSRRVKRLHKFFFKDKNEDSLEVIFFTKTYLQRKLKVGEWYLINGR
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHCCCCEEEEECC
MGVSAQMFHPDKILKLSERDKLSAFEPKYRLSDGITNYQFLSLVDSLLKSSKPPKEWLEQ
CCCEEEEECCHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
TSVRKRGLISWGKAIEQLHHPSSEVEFEQACKRLAYDEILAMHLVNNSLHTQVLNQKKEP
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
IKGDGRITALLRSRLPFSLTEGQEEVIKKIYALQGQDVRMVALLQGDVGSGKTLVVIFAM
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHH
LNAVEVGKQVVLLSPTIVLAKQHFEVLQKLLPELKPVLLTGEKIACSREQFLQDIRSGDA
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCHHHHHHHHHCCCC
KVIVATHAILAVGILFFDLGLLVIDEQHRFGANQRTKLIRENPGADLVLVSATPIPRTIG
EEEEHHHHHHHHHHHHHHCCCEEEECHHHCCCCHHHHHHHCCCCCCEEEEECCCCHHHHH
QVLFGSITLLNLREKPKSRPVVTTSTVTKSKIAKLIERIKVILGKGSKVFWICPVIEESE
HHHHHHEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
NLKVISVTERFRLLKKHFADEVDCVHGVLSAQKMEEKLTAFRNGKTKILLATTVVEVGVD
CEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHCCC
IPDVDVIVIEDADHFGLAQLHQLRGRVGRGNKKSFCILLYDSQKITENGKKRLKILRESS
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCHHHHHHHHEECC
DGFFIAEEDFKLRGVGDIFGVRQSGFYKFKFLDRPFEHSFFDSASMEAQDAINMGRIAQY
CCEEEEECCCEEECCHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHE
EVLIKVFFSDNNYYL
EEEEEEEECCCCCCC
>Mature Secondary Structure 
ANLFDPIDKIFLLEERMLEVVRNLVSGSRVVDLLLHKPRGFVNRCNFKAIANSVEGEFV
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCHHHHHHCCCCCEE
TIEARYEGSKEHFHRSRRVKRLHKFFFKDKNEDSLEVIFFTKTYLQRKLKVGEWYLINGR
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHCCCCEEEEECC
MGVSAQMFHPDKILKLSERDKLSAFEPKYRLSDGITNYQFLSLVDSLLKSSKPPKEWLEQ
CCCEEEEECCHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
TSVRKRGLISWGKAIEQLHHPSSEVEFEQACKRLAYDEILAMHLVNNSLHTQVLNQKKEP
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
IKGDGRITALLRSRLPFSLTEGQEEVIKKIYALQGQDVRMVALLQGDVGSGKTLVVIFAM
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHH
LNAVEVGKQVVLLSPTIVLAKQHFEVLQKLLPELKPVLLTGEKIACSREQFLQDIRSGDA
HHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHCCHHHHHHHHHCCCC
KVIVATHAILAVGILFFDLGLLVIDEQHRFGANQRTKLIRENPGADLVLVSATPIPRTIG
EEEEHHHHHHHHHHHHHHCCCEEEECHHHCCCCHHHHHHHCCCCCCEEEEECCCCHHHHH
QVLFGSITLLNLREKPKSRPVVTTSTVTKSKIAKLIERIKVILGKGSKVFWICPVIEESE
HHHHHHEEEEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
NLKVISVTERFRLLKKHFADEVDCVHGVLSAQKMEEKLTAFRNGKTKILLATTVVEVGVD
CEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHCCC
IPDVDVIVIEDADHFGLAQLHQLRGRVGRGNKKSFCILLYDSQKITENGKKRLKILRESS
CCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHCHHHHHHHHEECC
DGFFIAEEDFKLRGVGDIFGVRQSGFYKFKFLDRPFEHSFFDSASMEAQDAINMGRIAQY
CCEEEEECCCEEECCHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHE
EVLIKVFFSDNNYYL
EEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830261; 11463916 [H]