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The map label for this gene is bioB

Identifier: 88608192

GI number: 88608192

Start: 534862

End: 535863

Strand: Reverse

Name: bioB

Synonym: NSE_0612

Alternate gene names: 88608192

Gene position: 535863-534862 (Counterclockwise)

Preceding gene: 88608302

Following gene: 88608541

Centisome position: 62.38

GC content: 43.21

Gene sequence:

>1002_bases
ATGGCACCGGTTCCAGCAGGTATTATGGCGAAAGTTTGGCAATTCTCTGAGGCTGAGGAGTTATTCCACCTTCCTTTTTT
AGATCTTCTTTATTCTGCTCAACATGTGCATAGGGAAAATTTTCCACATAATGAGGTGCAGCTCGCTATGTTGCTCAGTG
TTAAGACTGGTGGGTGTCCGGAAAATTGCTCCTACTGTCCACAGTCTGCACACTATGACACAGGACTGAGAAAGGAACCC
GTAATGCATGTCGAAAAGGTACTTGAGGCAGCGAAGAGAGCTGTTGAGCTTGGTGCCACGAGATTCTGTATTGGTGCTGC
CTGGCGCGGACCGCGTGGGAAAGATTTGGATATAGTCTGTGAAATGATTTCCAGAATCAAGAAGCTCGGATTAGAAACTT
GCGCTTCGCTGGGCCTTCTCTCTTACGAACAAGCTGTTTCCCTGAAAGCAGCTGGTTTGGATTTTTACAACCACAATATT
GATACCTCACAGGAATATTATTCTAAAATAATAACTACCAGAAGGTTCAGCGACAGGATAGAAACCCTAGAGAATGTTGC
GAAAGCTGGTTTAAAAATCTGCTCTGGAGGTATTTTAGGGATGGGTGAGAGTAATGAAGACCGCATAAAGATGCTTGTTG
AGCTTTCTCACCTGAGTTTTCCACCCGAATCAATACCCATAAATAAGCTCATTCCGATTCCGGGGACTCCACTAGCGGAA
AAAAATGCTGTTGATCCTTTAGATTTCGTAAGGATTATCGCGCTTGCGAGGATTATGTTTCCTAAATCCTATGTGAGGCT
TTCTGCAGGTAGGGAAAGCATGTCAGATGAATTACAAACTTTATGTTTTATTGCCGGTGCTAATTCCATTTTCTATGGTG
AGAAGCTTCTTACATCTGCCAACTCTGACCCAGAGAGCGACGAGAAACTCTTATTGAAATTAGGTCTTCTGAGAGAGAAA
AGTAAAACAGCTGTTGATTGTTCCATTAATTTGGGATCATAG

Upstream 100 bases:

>100_bases
GACTGACTTGCATAAGGGGAGATTAGGTCTGGAAATGTTGAATGAGGTATCGATACGTTATTGGATAGGAAGATTCATTT
CTCTTAACCAGGGTGGTACA

Downstream 100 bases:

>100_bases
AGAAAGCAGCTTGCCTAAGGATTTTTTTCTGGGATGTGTAATACCTCTGTGTGTCTGTTTAAGTCATACAAGAAATAAAT
CCGTTCTAAAGGAATGGTAG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAPVPAGIMAKVWQFSEAEELFHLPFLDLLYSAQHVHRENFPHNEVQLAMLLSVKTGGCPENCSYCPQSAHYDTGLRKEP
VMHVEKVLEAAKRAVELGATRFCIGAAWRGPRGKDLDIVCEMISRIKKLGLETCASLGLLSYEQAVSLKAAGLDFYNHNI
DTSQEYYSKIITTRRFSDRIETLENVAKAGLKICSGGILGMGESNEDRIKMLVELSHLSFPPESIPINKLIPIPGTPLAE
KNAVDPLDFVRIIALARIMFPKSYVRLSAGRESMSDELQTLCFIAGANSIFYGEKLLTSANSDPESDEKLLLKLGLLREK
SKTAVDCSINLGS

Sequences:

>Translated_333_residues
MAPVPAGIMAKVWQFSEAEELFHLPFLDLLYSAQHVHRENFPHNEVQLAMLLSVKTGGCPENCSYCPQSAHYDTGLRKEP
VMHVEKVLEAAKRAVELGATRFCIGAAWRGPRGKDLDIVCEMISRIKKLGLETCASLGLLSYEQAVSLKAAGLDFYNHNI
DTSQEYYSKIITTRRFSDRIETLENVAKAGLKICSGGILGMGESNEDRIKMLVELSHLSFPPESIPINKLIPIPGTPLAE
KNAVDPLDFVRIIALARIMFPKSYVRLSAGRESMSDELQTLCFIAGANSIFYGEKLLTSANSDPESDEKLLLKLGLLREK
SKTAVDCSINLGS
>Mature_332_residues
APVPAGIMAKVWQFSEAEELFHLPFLDLLYSAQHVHRENFPHNEVQLAMLLSVKTGGCPENCSYCPQSAHYDTGLRKEPV
MHVEKVLEAAKRAVELGATRFCIGAAWRGPRGKDLDIVCEMISRIKKLGLETCASLGLLSYEQAVSLKAAGLDFYNHNID
TSQEYYSKIITTRRFSDRIETLENVAKAGLKICSGGILGMGESNEDRIKMLVELSHLSFPPESIPINKLIPIPGTPLAEK
NAVDPLDFVRIIALARIMFPKSYVRLSAGRESMSDELQTLCFIAGANSIFYGEKLLTSANSDPESDEKLLLKLGLLREKS
KTAVDCSINLGS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=313, Percent_Identity=54.6325878594249, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=47.2843450479233, Blast_Score=313, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36748; Mature: 36617

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPVPAGIMAKVWQFSEAEELFHLPFLDLLYSAQHVHRENFPHNEVQLAMLLSVKTGGCP
CCCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCC
ENCSYCPQSAHYDTGLRKEPVMHVEKVLEAAKRAVELGATRFCIGAAWRGPRGKDLDIVC
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHH
EMISRIKKLGLETCASLGLLSYEQAVSLKAAGLDFYNHNIDTSQEYYSKIITTRRFSDRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
ETLENVAKAGLKICSGGILGMGESNEDRIKMLVELSHLSFPPESIPINKLIPIPGTPLAE
HHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
KNAVDPLDFVRIIALARIMFPKSYVRLSAGRESMSDELQTLCFIAGANSIFYGEKLLTSA
CCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
NSDPESDEKLLLKLGLLREKSKTAVDCSINLGS
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure 
APVPAGIMAKVWQFSEAEELFHLPFLDLLYSAQHVHRENFPHNEVQLAMLLSVKTGGCP
CCCCHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCC
ENCSYCPQSAHYDTGLRKEPVMHVEKVLEAAKRAVELGATRFCIGAAWRGPRGKDLDIVC
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHH
EMISRIKKLGLETCASLGLLSYEQAVSLKAAGLDFYNHNIDTSQEYYSKIITTRRFSDRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
ETLENVAKAGLKICSGGILGMGESNEDRIKMLVELSHLSFPPESIPINKLIPIPGTPLAE
HHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
KNAVDPLDFVRIIALARIMFPKSYVRLSAGRESMSDELQTLCFIAGANSIFYGEKLLTSA
CCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
NSDPESDEKLLLKLGLLREKSKTAVDCSINLGS
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA