Click here to switch to the map view.

The map label for this gene is purD

Identifier: 88608190

GI number: 88608190

Start: 818998

End: 820263

Strand: Direct

Name: purD

Synonym: NSE_0924

Alternate gene names: 88608190

Gene position: 818998-820263 (Clockwise)

Preceding gene: 88608105

Following gene: 88608025

Centisome position: 95.34

GC content: 42.34

Gene sequence:

>1266_bases
ATGCAATTAAAAAAAGTTCTTATCGTCGGAAACGGCGCAAGGGAGCACGCTTTGATCACGAAAATCAAGGAATCACCGAT
GCTTGACAAGGTTTATACTACGAACTCGAATTTCGGTCAGTTCGCAGAATATCTTGATACAAACCTAGAAAATTATTTGT
ACACGTCGGCTACTTGCAAGACGGAAGGAATAGACCTAGTGGTAATAGGGCAGGAAGAACATTTGGCAAGAGGCATAACG
GATGCTCTTACGGCTGAAGGTATTCCTGTTTTTGGGCCCACAAAAAATGCAGCAAAATTAGAATCGTCGAAGTTTTTTGC
TAAGGAAATAGCAGAAATGAACGGTATACCTGTAGCCAGGTACAGTTTTTGTTCATCCATCAACAACCTGAAAAAGCAGA
TTGAAAATATTGGAAGTTTCCCTCTGGTTATTAAGGCCGATGGACTTGCATCCGGAAAAGGCGTCATAGTGTGTGAAACT
ATGGGAGAAGCACTAGAAGCAGGTGAAGCAATGCTAAATGGTTTATTTGGCGAAGCAGGCAGAAATATAATAATAGAAGA
GTTCTTATATGGGCAGGAATTAAGTTATTTTGTCCTGGTTGATGGACAGACTATTCTTCCAATTGGCCACGCGAGGGATC
ACAAACAAATCTCATACAACGGAAAAAAGTATAACACCGGTGGAATGGGAGCATACTCGCCCGTGCTGCTCTCCAAAAAA
ACTGAACAAGAAATACTTGGGAAGATTGTATATCCAACTATCAATGCATTGTCAGCAATTAACGTTCAGTACAAGGGAGT
CCTCTTTGCCGGACTCATGTTGACCAACTCTGGACCTAAACTATTGGAATATAATGTCCGATTTGGTGATCCAGAAACCC
AAGCAATACTACCACGCCTCAATGCCGATCTCCTTGATCTTATGCTCAAAACATTAGATGGGGGTCTTGACAGAGCTACG
ATTTCCTTCAAGAAAGAAGTGTGTATGTCTGTTGTTCTGGCAACCCGTGGGTATCCAGTCTCCTATGAAACCGGATACCG
CATAGATAATATAGAAAAGGCACTGCAAAGCGTTGAAGGACTCCAGATTCTCAACGCTGCTGTGAGATACGATGCAGAAG
GACGGATGATCTCTAACGGTGGTCGTGTTCTAAACTTAGTTGTAAGCGCCCCAACGTTATTGGAGTGCAAACGTAAAGTC
AAACGTGGCTTGGAACTAATAGACTGGAAAGAGGGAGTTTACTTAGATGAATTAGTCGTAGTGTAA

Upstream 100 bases:

>100_bases
AGAGAGAACACAAAGAAAACAGACAAACAAAAAGTTATATAGCTTTCTTCTCATTCGGGTAATGACGAACTACAATATGA
CCGTTGATTATAATCCGGCA

Downstream 100 bases:

>100_bases
GACCCAGAGAGAGCCTTTTCACAGCGATGTTTCACCGAGACGCCATAGCTATATCGTCTGTGGAATTCACGAGTGCATAG
CGGCTTCGGCACAGTCAAAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MQLKKVLIVGNGAREHALITKIKESPMLDKVYTTNSNFGQFAEYLDTNLENYLYTSATCKTEGIDLVVIGQEEHLARGIT
DALTAEGIPVFGPTKNAAKLESSKFFAKEIAEMNGIPVARYSFCSSINNLKKQIENIGSFPLVIKADGLASGKGVIVCET
MGEALEAGEAMLNGLFGEAGRNIIIEEFLYGQELSYFVLVDGQTILPIGHARDHKQISYNGKKYNTGGMGAYSPVLLSKK
TEQEILGKIVYPTINALSAINVQYKGVLFAGLMLTNSGPKLLEYNVRFGDPETQAILPRLNADLLDLMLKTLDGGLDRAT
ISFKKEVCMSVVLATRGYPVSYETGYRIDNIEKALQSVEGLQILNAAVRYDAEGRMISNGGRVLNLVVSAPTLLECKRKV
KRGLELIDWKEGVYLDELVVV

Sequences:

>Translated_421_residues
MQLKKVLIVGNGAREHALITKIKESPMLDKVYTTNSNFGQFAEYLDTNLENYLYTSATCKTEGIDLVVIGQEEHLARGIT
DALTAEGIPVFGPTKNAAKLESSKFFAKEIAEMNGIPVARYSFCSSINNLKKQIENIGSFPLVIKADGLASGKGVIVCET
MGEALEAGEAMLNGLFGEAGRNIIIEEFLYGQELSYFVLVDGQTILPIGHARDHKQISYNGKKYNTGGMGAYSPVLLSKK
TEQEILGKIVYPTINALSAINVQYKGVLFAGLMLTNSGPKLLEYNVRFGDPETQAILPRLNADLLDLMLKTLDGGLDRAT
ISFKKEVCMSVVLATRGYPVSYETGYRIDNIEKALQSVEGLQILNAAVRYDAEGRMISNGGRVLNLVVSAPTLLECKRKV
KRGLELIDWKEGVYLDELVVV
>Mature_421_residues
MQLKKVLIVGNGAREHALITKIKESPMLDKVYTTNSNFGQFAEYLDTNLENYLYTSATCKTEGIDLVVIGQEEHLARGIT
DALTAEGIPVFGPTKNAAKLESSKFFAKEIAEMNGIPVARYSFCSSINNLKKQIENIGSFPLVIKADGLASGKGVIVCET
MGEALEAGEAMLNGLFGEAGRNIIIEEFLYGQELSYFVLVDGQTILPIGHARDHKQISYNGKKYNTGGMGAYSPVLLSKK
TEQEILGKIVYPTINALSAINVQYKGVLFAGLMLTNSGPKLLEYNVRFGDPETQAILPRLNADLLDLMLKTLDGGLDRAT
ISFKKEVCMSVVLATRGYPVSYETGYRIDNIEKALQSVEGLQILNAAVRYDAEGRMISNGGRVLNLVVSAPTLLECKRKV
KRGLELIDWKEGVYLDELVVV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=418, Percent_Identity=37.799043062201, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI4503915, Length=418, Percent_Identity=37.799043062201, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI209869995, Length=418, Percent_Identity=37.799043062201, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI209869993, Length=418, Percent_Identity=37.799043062201, Blast_Score=262, Evalue=4e-70,
Organism=Escherichia coli, GI1790438, Length=421, Percent_Identity=38.479809976247, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17567511, Length=404, Percent_Identity=36.3861386138614, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321203, Length=434, Percent_Identity=35.4838709677419, Blast_Score=242, Evalue=7e-65,
Organism=Drosophila melanogaster, GI62471619, Length=408, Percent_Identity=36.5196078431373, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24582400, Length=408, Percent_Identity=36.5196078431373, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46082; Mature: 46082

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKKVLIVGNGAREHALITKIKESPMLDKVYTTNSNFGQFAEYLDTNLENYLYTSATCK
CCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCHHHEEEECCCCC
TEGIDLVVIGQEEHLARGITDALTAEGIPVFGPTKNAAKLESSKFFAKEIAEMNGIPVAR
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
YSFCSSINNLKKQIENIGSFPLVIKADGLASGKGVIVCETMGEALEAGEAMLNGLFGEAG
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCC
RNIIIEEFLYGQELSYFVLVDGQTILPIGHARDHKQISYNGKKYNTGGMGAYSPVLLSKK
CCEEEHHHHCCCCCEEEEEECCCEEEECCCCCCCCEEEECCCEECCCCCCCCCCHHCCCC
TEQEILGKIVYPTINALSAINVQYKGVLFAGLMLTNSGPKLLEYNVRFGDPETQAILPRL
HHHHHHHHHHHHHHHHHHEECEEECCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHCC
NADLLDLMLKTLDGGLDRATISFKKEVCMSVVLATRGYPVSYETGYRIDNIEKALQSVEG
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LQILNAAVRYDAEGRMISNGGRVLNLVVSAPTLLECKRKVKRGLELIDWKEGVYLDELVV
HHHHHHHHEECCCCCEEECCCEEEEEEECCCHHHHHHHHHHCCCEEEECCCCCCHHHEEE
V
C
>Mature Secondary Structure
MQLKKVLIVGNGAREHALITKIKESPMLDKVYTTNSNFGQFAEYLDTNLENYLYTSATCK
CCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCHHHEEEECCCCC
TEGIDLVVIGQEEHLARGITDALTAEGIPVFGPTKNAAKLESSKFFAKEIAEMNGIPVAR
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
YSFCSSINNLKKQIENIGSFPLVIKADGLASGKGVIVCETMGEALEAGEAMLNGLFGEAG
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCC
RNIIIEEFLYGQELSYFVLVDGQTILPIGHARDHKQISYNGKKYNTGGMGAYSPVLLSKK
CCEEEHHHHCCCCCEEEEEECCCEEEECCCCCCCCEEEECCCEECCCCCCCCCCHHCCCC
TEQEILGKIVYPTINALSAINVQYKGVLFAGLMLTNSGPKLLEYNVRFGDPETQAILPRL
HHHHHHHHHHHHHHHHHHEECEEECCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHCC
NADLLDLMLKTLDGGLDRATISFKKEVCMSVVLATRGYPVSYETGYRIDNIEKALQSVEG
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LQILNAAVRYDAEGRMISNGGRVLNLVVSAPTLLECKRKVKRGLELIDWKEGVYLDELVV
HHHHHHHHEECCCCCEEECCCEEEEEEECCCHHHHHHHHHHCCCEEEECCCCCCHHHEEE
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]