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The map label for this gene is petB

Identifier: 88608151

GI number: 88608151

Start: 546558

End: 547790

Strand: Direct

Name: petB

Synonym: NSE_0625

Alternate gene names: 88608151

Gene position: 546558-547790 (Clockwise)

Preceding gene: 88608022

Following gene: 88608753

Centisome position: 63.63

GC content: 40.79

Gene sequence:

>1233_bases
ATGTCTGATACTGAAGCTACTCTAAAGGGGAATTCTGGTGTTGTAGGATGGATAGAACATAGGTTGCCCATCTTCTCGTT
TTTAAAGGGGTTTTTTGTCGATTATAGGGCACCAAAGAACCTGAATATTTTTTGGAATTTCGGTATATTAGCTGCGCTGT
CACTGGGGATTCAGCTTCTGACAGGGATATTTCTGGCTATGCATTACGATCCAAATGCTGCTAATGCGTTCGCGAGTGTG
GAACATATAACTAGGGATGTTAATTTTGGATGGCTACTTAGATACTCACATCAGGTTGGAGCATCGATGTTTTTCGCCGT
GGTATACATTCATATCTTCAGGGGACTGTATTATGGATCATATAAAAACCCGCGCGAACTACTGTGGATAATCGGAATCG
TAATATACTTATGCATGATGGCCACTAGCTTCCTTGGATATGTCCTCCCATGGGGACAGATGAGTTTTTGGGCAGCAACT
GTGATAACTAATTTTTTTTCAGCAATCCCTGTTTTTGGAGAGTCAATAGCACAGTGGATTTGGGGTGGATTTGCTGTGGG
AAATCCTACCTTGAATAAGTTTTTTGCCTTACATTACCTTTTTCCATTCATCATAGTTGCACTGGCTTGTCTGCACATGA
TTGCATTACATAGGTTTGGTTCGAATAATCCGTCTGGGATAGAAGTAGCCTCTACAAGAGAAACCGTGCCTTTTCATCCG
TATTATACTGCTAAGGACTTTGTAACAGTTGTGCTGTTTTTCCTTGTATTCTCAGGGTTTGTATTTTTTGCACCTGACTA
CTTAGGGCACCCAGATAATTTTATTGAGGCAGACCCATTGGTTACACCAAGTCATATAGTGCCGGAGTGGTATTTCCTCC
CTTTTTATGCAATCTTACGTGCAATTCCAAATAAACTTGGAGGAGTGGTAGCATTGCTTTCCTCAGTTCTAATACTGATA
GCAGTACCATGGTTGGATACTTCAAAAGTTAAAAGTGGTAGGTACCGTCCGCTTTTTAAAAAGTTCTTCTTTGTCTTCTT
AGTGAATTTTCTATTTTTGCTTTGGTTAGGAGGTAGAGAAGCTGTGGAACCATACATAAGTATGAGTAGAGTAGCGACGT
TATACTACTTCGGTTATTTCATGGTTGTGCTGCCTATCTTAGGGAAGCTTGAGAAAACGCTACCGCTACCAAAAAGTATT
GAGGAAAGCCTCAAGGAGAGCAACAAAGAGTAG

Upstream 100 bases:

>100_bases
TCCGGCAGGGTTGTTTCCGGTCCAGCGCCGACTAATCTTGTTGTGCCGGATTATTATTTCATCAACGATACAACAGTTGT
GGTAGGAGCAAAGGAAAAAA

Downstream 100 bases:

>100_bases
GTCTGTGATATGAAAAAGTATTTTTTTATTGCTCTTTTGCTCTGCCAAGTTTCTGCACTTGCGAGTGAAGGAAGCTTTGA
ACCAAAAGAGCTCGTTAAAA

Product: ubiquinol-cytochrome c reductase, cytochrome b

Products: Q; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSDTEATLKGNSGVVGWIEHRLPIFSFLKGFFVDYRAPKNLNIFWNFGILAALSLGIQLLTGIFLAMHYDPNAANAFASV
EHITRDVNFGWLLRYSHQVGASMFFAVVYIHIFRGLYYGSYKNPRELLWIIGIVIYLCMMATSFLGYVLPWGQMSFWAAT
VITNFFSAIPVFGESIAQWIWGGFAVGNPTLNKFFALHYLFPFIIVALACLHMIALHRFGSNNPSGIEVASTRETVPFHP
YYTAKDFVTVVLFFLVFSGFVFFAPDYLGHPDNFIEADPLVTPSHIVPEWYFLPFYAILRAIPNKLGGVVALLSSVLILI
AVPWLDTSKVKSGRYRPLFKKFFFVFLVNFLFLLWLGGREAVEPYISMSRVATLYYFGYFMVVLPILGKLEKTLPLPKSI
EESLKESNKE

Sequences:

>Translated_410_residues
MSDTEATLKGNSGVVGWIEHRLPIFSFLKGFFVDYRAPKNLNIFWNFGILAALSLGIQLLTGIFLAMHYDPNAANAFASV
EHITRDVNFGWLLRYSHQVGASMFFAVVYIHIFRGLYYGSYKNPRELLWIIGIVIYLCMMATSFLGYVLPWGQMSFWAAT
VITNFFSAIPVFGESIAQWIWGGFAVGNPTLNKFFALHYLFPFIIVALACLHMIALHRFGSNNPSGIEVASTRETVPFHP
YYTAKDFVTVVLFFLVFSGFVFFAPDYLGHPDNFIEADPLVTPSHIVPEWYFLPFYAILRAIPNKLGGVVALLSSVLILI
AVPWLDTSKVKSGRYRPLFKKFFFVFLVNFLFLLWLGGREAVEPYISMSRVATLYYFGYFMVVLPILGKLEKTLPLPKSI
EESLKESNKE
>Mature_409_residues
SDTEATLKGNSGVVGWIEHRLPIFSFLKGFFVDYRAPKNLNIFWNFGILAALSLGIQLLTGIFLAMHYDPNAANAFASVE
HITRDVNFGWLLRYSHQVGASMFFAVVYIHIFRGLYYGSYKNPRELLWIIGIVIYLCMMATSFLGYVLPWGQMSFWAATV
ITNFFSAIPVFGESIAQWIWGGFAVGNPTLNKFFALHYLFPFIIVALACLHMIALHRFGSNNPSGIEVASTRETVPFHPY
YTAKDFVTVVLFFLVFSGFVFFAPDYLGHPDNFIEADPLVTPSHIVPEWYFLPFYAILRAIPNKLGGVVALLSSVLILIA
VPWLDTSKVKSGRYRPLFKKFFFVFLVNFLFLLWLGGREAVEPYISMSRVATLYYFGYFMVVLPILGKLEKTLPLPKSIE
ESLKESNKE

Specific function: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis [H]

COG id: COG1290

COG function: function code C; Cytochrome b subunit of the bc complex

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b family [H]

Homologues:

Organism=Homo sapiens, GI251831119, Length=372, Percent_Identity=50, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6226529, Length=370, Percent_Identity=50.2702702702703, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6226532, Length=245, Percent_Identity=52.2448979591837, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6226531, Length=165, Percent_Identity=52.1212121212121, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6226530, Length=148, Percent_Identity=46.6216216216216, Blast_Score=136, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016175
- InterPro:   IPR005798
- InterPro:   IPR005797
- InterPro:   IPR016174 [H]

Pfam domain/function: PF00032 Cytochrom_B_C; PF00033 Cytochrom_B_N [H]

EC number: 1.10.2.2

Molecular weight: Translated: 46570; Mature: 46439

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS51002 CYTB_NTER ; PS51003 CYTB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTEATLKGNSGVVGWIEHRLPIFSFLKGFFVDYRAPKNLNIFWNFGILAALSLGIQLL
CCCCCCEECCCCCCEEHHHHCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
TGIFLAMHYDPNAANAFASVEHITRDVNFGWLLRYSHQVGASMFFAVVYIHIFRGLYYGS
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YKNPRELLWIIGIVIYLCMMATSFLGYVLPWGQMSFWAATVITNFFSAIPVFGESIAQWI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGGFAVGNPTLNKFFALHYLFPFIIVALACLHMIALHRFGSNNPSGIEVASTRETVPFHP
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
YYTAKDFVTVVLFFLVFSGFVFFAPDYLGHPDNFIEADPLVTPSHIVPEWYFLPFYAILR
CCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
AIPNKLGGVVALLSSVLILIAVPWLDTSKVKSGRYRPLFKKFFFVFLVNFLFLLWLGGRE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
AVEPYISMSRVATLYYFGYFMVVLPILGKLEKTLPLPKSIEESLKESNKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SDTEATLKGNSGVVGWIEHRLPIFSFLKGFFVDYRAPKNLNIFWNFGILAALSLGIQLL
CCCCCEECCCCCCEEHHHHCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
TGIFLAMHYDPNAANAFASVEHITRDVNFGWLLRYSHQVGASMFFAVVYIHIFRGLYYGS
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YKNPRELLWIIGIVIYLCMMATSFLGYVLPWGQMSFWAATVITNFFSAIPVFGESIAQWI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGGFAVGNPTLNKFFALHYLFPFIIVALACLHMIALHRFGSNNPSGIEVASTRETVPFHP
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCC
YYTAKDFVTVVLFFLVFSGFVFFAPDYLGHPDNFIEADPLVTPSHIVPEWYFLPFYAILR
CCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
AIPNKLGGVVALLSSVLILIAVPWLDTSKVKSGRYRPLFKKFFFVFLVNFLFLLWLGGRE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
AVEPYISMSRVATLYYFGYFMVVLPILGKLEKTLPLPKSIEESLKESNKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: QH2; ferricytochrome c

Specific reaction: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]