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The map label for this gene is hslV

Identifier: 88608110

GI number: 88608110

Start: 145277

End: 145843

Strand: Direct

Name: hslV

Synonym: NSE_0176

Alternate gene names: 88608110

Gene position: 145277-145843 (Clockwise)

Preceding gene: 88607993

Following gene: 88607962

Centisome position: 16.91

GC content: 44.27

Gene sequence:

>567_bases
ATGGGTACTGGGTTTCATGGGACTACTATTCTTTCGATCAGAAAGGATGACAAGGTCGTCATGATAGGTGATGGACAAGT
GACTATGGGAAATGCAGTCGTTGTAAAATCGACTGCACAGAAAGTGAAGCGTCTTTCAAATGGGAAGATAATATCGGGTT
TTGCTGGATCAACTGCGGATGCATTCACATTATTTGAACGCCTAGAGTCAAAATTGGAAGCTCATCCGGGGCAACTTCTG
CGTGCGTGTGTGGAACTTGCGAAAGATTGGCGTACGGATAAATTTCTTCGTCGGTTAGAGGCGATGATGATTGTAGCTGA
TGCTAGGGGTACGCTCATTCTGAATGGTGCTGGTGATGTGATAGAGCCTGAGGATAGTGTTGCTGCTATTGGATCTGGGG
GCAACTATGCTCTAGCTGCTGCTAAAGCGCTTATAGTTCACGCCAGTGATCTTGATGCTTTTCAGATTGCTGAAGCGGCT
ATGAAAATAGCTGCTAAGATCTGCGTTTTTACAAATGAAAATTTTACGGTTGAAGTCATCGATTGTGCTTCAAAAGAGTG
TAGTTGA

Upstream 100 bases:

>100_bases
GATTGGTGCAAAAGATGTATTGGATGAGCGCGGAGTGTTAGCAGAAGCGCTGTAGCATTCATTCGTGTAACCAATTTAGT
TTAGCTTTTGGAGATATAAA

Downstream 100 bases:

>100_bases
AGTTAGTGAGAGTTTAAAACATGGGTACAGAGAAAGTCTTTAAGTATTCAGCTGCGGTTGGGCAGGATGCCGAACATATC
TTGGAGGATGCTTATTCTGC

Product: ATP-dependent protease peptidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MGTGFHGTTILSIRKDDKVVMIGDGQVTMGNAVVVKSTAQKVKRLSNGKIISGFAGSTADAFTLFERLESKLEAHPGQLL
RACVELAKDWRTDKFLRRLEAMMIVADARGTLILNGAGDVIEPEDSVAAIGSGGNYALAAAKALIVHASDLDAFQIAEAA
MKIAAKICVFTNENFTVEVIDCASKECS

Sequences:

>Translated_188_residues
MGTGFHGTTILSIRKDDKVVMIGDGQVTMGNAVVVKSTAQKVKRLSNGKIISGFAGSTADAFTLFERLESKLEAHPGQLL
RACVELAKDWRTDKFLRRLEAMMIVADARGTLILNGAGDVIEPEDSVAAIGSGGNYALAAAKALIVHASDLDAFQIAEAA
MKIAAKICVFTNENFTVEVIDCASKECS
>Mature_187_residues
GTGFHGTTILSIRKDDKVVMIGDGQVTMGNAVVVKSTAQKVKRLSNGKIISGFAGSTADAFTLFERLESKLEAHPGQLLR
ACVELAKDWRTDKFLRRLEAMMIVADARGTLILNGAGDVIEPEDSVAAIGSGGNYALAAAKALIVHASDLDAFQIAEAAM
KIAAKICVFTNENFTVEVIDCASKECS

Specific function: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery

COG id: COG5405

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), peptidase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase T1B family. HslV subfamily

Homologues:

Organism=Escherichia coli, GI1790367, Length=171, Percent_Identity=56.7251461988304, Blast_Score=197, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLV_NEOSM (Q2GEM5)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506071.1
- ProteinModelPortal:   Q2GEM5
- SMR:   Q2GEM5
- STRING:   Q2GEM5
- MEROPS:   T01.006
- GeneID:   3931981
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0176
- TIGR:   NSE_0176
- eggNOG:   COG5405
- HOGENOM:   HBG288822
- OMA:   AADICVY
- PhylomeDB:   Q2GEM5
- ProtClustDB:   PRK05456
- BioCyc:   NSEN222891:NSE_0176-MONOMER
- HAMAP:   MF_00248
- InterPro:   IPR022281
- InterPro:   IPR001353
- TIGRFAMs:   TIGR03692

Pfam domain/function: PF00227 Proteasome

EC number: 3.4.25.-

Molecular weight: Translated: 19935; Mature: 19804

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: ACT_SITE 8-8

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTGFHGTTILSIRKDDKVVMIGDGQVTMGNAVVVKSTAQKVKRLSNGKIISGFAGSTAD
CCCCCCCEEEEEEECCCCEEEEECCEEEECCEEEEEHHHHHHHHHCCCCEEECCCCCCHH
AFTLFERLESKLEAHPGQLLRACVELAKDWRTDKFLRRLEAMMIVADARGTLILNGAGDV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCEEEECCCCCC
IEPEDSVAAIGSGGNYALAAAKALIVHASDLDAFQIAEAAMKIAAKICVFTNENFTVEVI
CCCCCCEEEECCCCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEEE
DCASKECS
ECCCCCCC
>Mature Secondary Structure 
GTGFHGTTILSIRKDDKVVMIGDGQVTMGNAVVVKSTAQKVKRLSNGKIISGFAGSTAD
CCCCCCEEEEEEECCCCEEEEECCEEEECCEEEEEHHHHHHHHHCCCCEEECCCCCCHH
AFTLFERLESKLEAHPGQLLRACVELAKDWRTDKFLRRLEAMMIVADARGTLILNGAGDV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCEEEECCCCCC
IEPEDSVAAIGSGGNYALAAAKALIVHASDLDAFQIAEAAMKIAAKICVFTNENFTVEVI
CCCCCCEEEECCCCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHEEEEECCCCEEEEE
DCASKECS
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA