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The map label for this gene is pepQ [H]

Identifier: 88608080

GI number: 88608080

Start: 388285

End: 389922

Strand: Direct

Name: pepQ [H]

Synonym: NSE_0471

Alternate gene names: 88608080

Gene position: 388285-389922 (Clockwise)

Preceding gene: 88608300

Following gene: 88608344

Centisome position: 45.2

GC content: 40.29

Gene sequence:

>1638_bases
GTGATAACGCCTGAAGAGAAGTTGTCTGCTCTGAGGAGAATAATGGAAGATAACCGGATGGAGGGTTTTCTTATAACTAT
TAGTGATGAATTCTTACTTGAGTCTCCTCTTTCGTATAATAACCGCATTAAGTGGCTTACTGGATTTTGTGGATCGTTTG
CTATGGTCCTCATTACTTGCGAGAAAGCGTATTTTTTTACCGATTCTAGGTATTTGATTCAAGTAAAGCTTGAAGTTTCT
GAAATATATACACGCCTGCAGTTTAGTCTTGCTGAAATTGAGCGTGTGATAAAGGAGAGTTGTATCCGGAGAATTTGTTA
CGATTCAAGATTGCTTAACCGTGCCATGTTGAGCTTCTTCCGGTGTCAGATGGAGCCTTTAGATTGGAATCCCATTGATT
GCCTCTGGGACAGAAATACAGTTTCTGTTGGAAGGGTTGTAGTTCATCCACTTTGTTATGCAGGGTTATCTAGTCAGGAA
AAGTGCAAACAAATCATCAACATAACAGGTGGGAATAACTATTTTTTCTCTAATTCAGAGTCCGTGTGCTGGTTAGCAAA
CATACGTGGTTCAGATCTTGAATATACACCTGTAGTTTGCTGCAGAGCCATTCTTTATTCGAATGGGCTGCTGAGAATCT
TTCTATGGGGCCAGGTAGAGGATTTACCTTCGTTGGAAAGTCATATAGAGATTTTGCGATTGGAAGAATTACAGTTTTAC
TTGACACAATTAAAATCGGTAGCTCTAGATGAGCAGAGCGTAAATATTTACTACTTGAATCTAATAAGAGATAGAGATAT
ACAAATTACTCATCTGCAGGATCCATCGGTTATTATGAGAGCTTGCAAGAACCACATAGAACTTGAAGGTAGTATAGCAG
CTCATAAAAGAGATGGCTTGGCTCTGACAAAGTTCTTAAACTGGCTCAAAGTAAATGAGTCTTCAGATGAATTAGCATCA
GCAGAGATGTTATTAAGTTTTAGAAAAGAGCAAGAGCTGTTTTTTTCCCTGAGTTTTCCTACGATTTCTGCATTTGGTCC
ACACGGTGCGATAGTTCACTATACTCCTTCTAAAAAGAGCAATTTGCAATTCAAGCCCGGAAATCTATATTTGGTCGACT
CCGGTGCTCAGTATCTGGATGGTACGACAGACGTGACACGTACTGTTGCAATAGGTGAGCCAACTGAGGAGCAAAAGTTT
CATTATACTATTGTCCTGAAGGCGCACATTGGGCTTGCTAAAGCTGTGTTTCCGGCTGGTACTACTGGTAGACAGTTAGA
TGTACTGGCTAGGTCTCACCTTTGGAGTTACAAACTTGATTATGCTCATGGTACTGGTCATGGTGTTGGGAGCTTTCTTA
ATGTTCATGAAGGGCCACATTCATTTGGAAGTGAGGTGCCGTTAAAAGTAGGTATGATTATTTCAAATGAACCTGGTCTC
TATTTCGAAGGGAAATATGGCATAAGACTGGAGAATCTTATGTATGTTAAGGAAGCTGGAGATGGGTTTTTGAGTTTTGC
TCCTCTTACTCTTGTTAATTTTGATGAGAATCTAATACGACACGAAATGCTTTCCGATAGCGAATCACGGTGGCTGGAGG
ATTACTCGGATTTAGTCAGGACAACACACAGGTCTTAG

Upstream 100 bases:

>100_bases
TTTCTTTTAAGAGCACAAAGCTTATGGTGATTTCTATGACCTGCATATCCAAAACCAGACAGTAATGTTAAAACACTTGT
TTTAATTTTCTGTGATTAGT

Downstream 100 bases:

>100_bases
CACTTTTTTCTTTGAAAAAGCAAAACAGACATACTCAGAAAACAACAAGTTAACTCTAATAACGCGCTCGGCTTACTTAC
TTTTTGCTAATCCGCACTTT

Product: M24 family metallopeptidase

Products: NA

Alternate protein names: X-Pro dipeptidase; Imidodipeptidase; Proline dipeptidase; Prolidase [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MITPEEKLSALRRIMEDNRMEGFLITISDEFLLESPLSYNNRIKWLTGFCGSFAMVLITCEKAYFFTDSRYLIQVKLEVS
EIYTRLQFSLAEIERVIKESCIRRICYDSRLLNRAMLSFFRCQMEPLDWNPIDCLWDRNTVSVGRVVVHPLCYAGLSSQE
KCKQIINITGGNNYFFSNSESVCWLANIRGSDLEYTPVVCCRAILYSNGLLRIFLWGQVEDLPSLESHIEILRLEELQFY
LTQLKSVALDEQSVNIYYLNLIRDRDIQITHLQDPSVIMRACKNHIELEGSIAAHKRDGLALTKFLNWLKVNESSDELAS
AEMLLSFRKEQELFFSLSFPTISAFGPHGAIVHYTPSKKSNLQFKPGNLYLVDSGAQYLDGTTDVTRTVAIGEPTEEQKF
HYTIVLKAHIGLAKAVFPAGTTGRQLDVLARSHLWSYKLDYAHGTGHGVGSFLNVHEGPHSFGSEVPLKVGMIISNEPGL
YFEGKYGIRLENLMYVKEAGDGFLSFAPLTLVNFDENLIRHEMLSDSESRWLEDYSDLVRTTHRS

Sequences:

>Translated_545_residues
MITPEEKLSALRRIMEDNRMEGFLITISDEFLLESPLSYNNRIKWLTGFCGSFAMVLITCEKAYFFTDSRYLIQVKLEVS
EIYTRLQFSLAEIERVIKESCIRRICYDSRLLNRAMLSFFRCQMEPLDWNPIDCLWDRNTVSVGRVVVHPLCYAGLSSQE
KCKQIINITGGNNYFFSNSESVCWLANIRGSDLEYTPVVCCRAILYSNGLLRIFLWGQVEDLPSLESHIEILRLEELQFY
LTQLKSVALDEQSVNIYYLNLIRDRDIQITHLQDPSVIMRACKNHIELEGSIAAHKRDGLALTKFLNWLKVNESSDELAS
AEMLLSFRKEQELFFSLSFPTISAFGPHGAIVHYTPSKKSNLQFKPGNLYLVDSGAQYLDGTTDVTRTVAIGEPTEEQKF
HYTIVLKAHIGLAKAVFPAGTTGRQLDVLARSHLWSYKLDYAHGTGHGVGSFLNVHEGPHSFGSEVPLKVGMIISNEPGL
YFEGKYGIRLENLMYVKEAGDGFLSFAPLTLVNFDENLIRHEMLSDSESRWLEDYSDLVRTTHRS
>Mature_545_residues
MITPEEKLSALRRIMEDNRMEGFLITISDEFLLESPLSYNNRIKWLTGFCGSFAMVLITCEKAYFFTDSRYLIQVKLEVS
EIYTRLQFSLAEIERVIKESCIRRICYDSRLLNRAMLSFFRCQMEPLDWNPIDCLWDRNTVSVGRVVVHPLCYAGLSSQE
KCKQIINITGGNNYFFSNSESVCWLANIRGSDLEYTPVVCCRAILYSNGLLRIFLWGQVEDLPSLESHIEILRLEELQFY
LTQLKSVALDEQSVNIYYLNLIRDRDIQITHLQDPSVIMRACKNHIELEGSIAAHKRDGLALTKFLNWLKVNESSDELAS
AEMLLSFRKEQELFFSLSFPTISAFGPHGAIVHYTPSKKSNLQFKPGNLYLVDSGAQYLDGTTDVTRTVAIGEPTEEQKF
HYTIVLKAHIGLAKAVFPAGTTGRQLDVLARSHLWSYKLDYAHGTGHGVGSFLNVHEGPHSFGSEVPLKVGMIISNEPGL
YFEGKYGIRLENLMYVKEAGDGFLSFAPLTLVNFDENLIRHEMLSDSESRWLEDYSDLVRTTHRS

Specific function: Splits dipeptides with a prolyl in the C-terminal position and a nonpolar amino acid at the N-terminal position [H]

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family. Archaeal-type prolidase subfamily [H]

Homologues:

Organism=Homo sapiens, GI264681563, Length=584, Percent_Identity=34.0753424657534, Blast_Score=303, Evalue=4e-82,
Organism=Homo sapiens, GI264681565, Length=584, Percent_Identity=32.3630136986301, Blast_Score=269, Evalue=6e-72,
Organism=Homo sapiens, GI93141226, Length=579, Percent_Identity=30.2245250431779, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1788728, Length=183, Percent_Identity=32.7868852459016, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509539, Length=588, Percent_Identity=32.1428571428571, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI25149105, Length=550, Percent_Identity=26, Blast_Score=164, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322999, Length=627, Percent_Identity=28.8676236044657, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17137632, Length=567, Percent_Identity=33.5097001763668, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI161078230, Length=581, Percent_Identity=26.8502581755594, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21357287, Length=581, Percent_Identity=26.8502581755594, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: =3.4.13.9 [H]

Molecular weight: Translated: 62199; Mature: 62199

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00491 PROLINE_PEPTIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPEEKLSALRRIMEDNRMEGFLITISDEFLLESPLSYNNRIKWLTGFCGSFAMVLITC
CCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCCEEEEEHHHHHHHEEEEEEE
EKAYFFTDSRYLIQVKLEVSEIYTRLQFSLAEIERVIKESCIRRICYDSRLLNRAMLSFF
CCEEEEECCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RCQMEPLDWNPIDCLWDRNTVSVGRVVVHPLCYAGLSSQEKCKQIINITGGNNYFFSNSE
HHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCEEEECCCC
SVCWLANIRGSDLEYTPVVCCRAILYSNGLLRIFLWGQVEDLPSLESHIEILRLEELQFY
CEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LTQLKSVALDEQSVNIYYLNLIRDRDIQITHLQDPSVIMRACKNHIELEGSIAAHKRDGL
HHHHHHHHCCCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHEEECCCEEECCCCCC
ALTKFLNWLKVNESSDELASAEMLLSFRKEQELFFSLSFPTISAFGPHGAIVHYTPSKKS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCEEEECCCCCC
NLQFKPGNLYLVDSGAQYLDGTTDVTRTVAIGEPTEEQKFHYTIVLKAHIGLAKAVFPAG
CEEECCCCEEEEECCCHHCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCHHHHHCCCC
TTGRQLDVLARSHLWSYKLDYAHGTGHGVGSFLNVHEGPHSFGSEVPLKVGMIISNEPGL
CCCCCHHHHHHHCCEEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEEEECCCCE
YFEGKYGIRLENLMYVKEAGDGFLSFAPLTLVNFDENLIRHEMLSDSESRWLEDYSDLVR
EEECCCCEEEEEEEEEEECCCCCEEECCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHH
TTHRS
HHCCC
>Mature Secondary Structure
MITPEEKLSALRRIMEDNRMEGFLITISDEFLLESPLSYNNRIKWLTGFCGSFAMVLITC
CCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCCEEEEEHHHHHHHEEEEEEE
EKAYFFTDSRYLIQVKLEVSEIYTRLQFSLAEIERVIKESCIRRICYDSRLLNRAMLSFF
CCEEEEECCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RCQMEPLDWNPIDCLWDRNTVSVGRVVVHPLCYAGLSSQEKCKQIINITGGNNYFFSNSE
HHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCEEEECCCC
SVCWLANIRGSDLEYTPVVCCRAILYSNGLLRIFLWGQVEDLPSLESHIEILRLEELQFY
CEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LTQLKSVALDEQSVNIYYLNLIRDRDIQITHLQDPSVIMRACKNHIELEGSIAAHKRDGL
HHHHHHHHCCCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHEEECCCEEECCCCCC
ALTKFLNWLKVNESSDELASAEMLLSFRKEQELFFSLSFPTISAFGPHGAIVHYTPSKKS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCEEEECCCCCC
NLQFKPGNLYLVDSGAQYLDGTTDVTRTVAIGEPTEEQKFHYTIVLKAHIGLAKAVFPAG
CEEECCCCEEEEECCCHHCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCHHHHHCCCC
TTGRQLDVLARSHLWSYKLDYAHGTGHGVGSFLNVHEGPHSFGSEVPLKVGMIISNEPGL
CCCCCHHHHHHHCCEEEEEEECCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEEEECCCCE
YFEGKYGIRLENLMYVKEAGDGFLSFAPLTLVNFDENLIRHEMLSDSESRWLEDYSDLVR
EEECCCCEEEEEEEEEEECCCCCEEECCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHH
TTHRS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9733678; 11223522; 11210522 [H]