Click here to switch to the map view.

The map label for this gene is dnaQ

Identifier: 88608300

GI number: 88608300

Start: 387389

End: 388075

Strand: Direct

Name: dnaQ

Synonym: NSE_0470

Alternate gene names: 88608300

Gene position: 387389-388075 (Clockwise)

Preceding gene: 88608468

Following gene: 88608080

Centisome position: 45.1

GC content: 39.16

Gene sequence:

>687_bases
ATGACTCAGAGACGTAAAGTTATTTTAGATACCGAGACCACGGGCCTCGATATGAAGAATGGAGATCGTGTCATTGAAAT
TGGCTGTGTCGAGGTGGTGGATTTTCGAATTACAGGCGAAATCTTTCACATTTATTTGAATCCTGAGCGTGAGATTTCAA
AAGAGGCAACACGTGTACACGGTATCACTTTCGAACAACTTGCTGATAGTCCAAAGTTCATCGATATAGCAGATGATTTT
CTAAAATTTATAGATTCATCAGAAATAGTCGCTCATAACGCAAGTTTTGATATCCGTTTTCTCAATTCTGAACTGATCCG
TGCAAGGCAGAGAAGATTGATCAAATTGGAAAATGTTATAGATACTCTTGCTATGGCAAGAAAGATATTTCCGGGTTCTC
CAGCTTCATTAGATGCACTATGTAGGAGATTTAGGATTTCACTTGAGCAGAGAGAGTTACACGGAGCTTTATTGGATGCA
CAGCTTTTAGCGCGTGTTTACATAGAATTAAGTAAGGGTAGCCAAGTCAGTATGAGTTTTTTAGAGGGATCTAGTGTAAG
TTCTGAGAAAAAAATGTTCAAAGAACGTGAGAAGCTCATTATTACCCCTGAGGAGTCGCTTGAGCATAGTGAACTGATCA
GGCGCCTTAAAAATCCTCTTTGGGATGAAATTTTGATGGAGGAATAA

Upstream 100 bases:

>100_bases
GAAAATTCAATGCATCCACTTGTTTACCTAGATCTGCAAAGGGACGGGCGGGTTACTTGTCCTTATTGTGGTGCTATATT
TGTTCACAGGGGTACAAACG

Downstream 100 bases:

>100_bases
AGTCATCTACGGACAGGATTCCGCTTTAACACGGTTTTTATGACCAATATACGCTGTGTACTTGTTTTAAGTCTATTGTG
ACTGTTAAAAGTAGTTTAGT

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTQRRKVILDTETTGLDMKNGDRVIEIGCVEVVDFRITGEIFHIYLNPEREISKEATRVHGITFEQLADSPKFIDIADDF
LKFIDSSEIVAHNASFDIRFLNSELIRARQRRLIKLENVIDTLAMARKIFPGSPASLDALCRRFRISLEQRELHGALLDA
QLLARVYIELSKGSQVSMSFLEGSSVSSEKKMFKEREKLIITPEESLEHSELIRRLKNPLWDEILMEE

Sequences:

>Translated_228_residues
MTQRRKVILDTETTGLDMKNGDRVIEIGCVEVVDFRITGEIFHIYLNPEREISKEATRVHGITFEQLADSPKFIDIADDF
LKFIDSSEIVAHNASFDIRFLNSELIRARQRRLIKLENVIDTLAMARKIFPGSPASLDALCRRFRISLEQRELHGALLDA
QLLARVYIELSKGSQVSMSFLEGSSVSSEKKMFKEREKLIITPEESLEHSELIRRLKNPLWDEILMEE
>Mature_227_residues
TQRRKVILDTETTGLDMKNGDRVIEIGCVEVVDFRITGEIFHIYLNPEREISKEATRVHGITFEQLADSPKFIDIADDFL
KFIDSSEIVAHNASFDIRFLNSELIRARQRRLIKLENVIDTLAMARKIFPGSPASLDALCRRFRISLEQRELHGALLDAQ
LLARVYIELSKGSQVSMSFLEGSSVSSEKKMFKEREKLIITPEESLEHSELIRRLKNPLWDEILMEE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786409, Length=188, Percent_Identity=48.4042553191489, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788149, Length=170, Percent_Identity=32.3529411764706, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006054
- InterPro:   IPR006309
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 26279; Mature: 26147

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRRKVILDTETTGLDMKNGDRVIEIGCVEVVDFRITGEIFHIYLNPEREISKEATRVH
CCCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHC
GITFEQLADSPKFIDIADDFLKFIDSSEIVAHNASFDIRFLNSELIRARQRRLIKLENVI
CCCHHHHCCCCCEEEHHHHHHHHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHH
DTLAMARKIFPGSPASLDALCRRFRISLEQRELHGALLDAQLLARVYIELSKGSQVSMSF
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LEGSSVSSEKKMFKEREKLIITPEESLEHSELIRRLKNPLWDEILMEE
CCCCCCCHHHHHHHHHCCEEECCHHCCCHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
TQRRKVILDTETTGLDMKNGDRVIEIGCVEVVDFRITGEIFHIYLNPEREISKEATRVH
CCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHC
GITFEQLADSPKFIDIADDFLKFIDSSEIVAHNASFDIRFLNSELIRARQRRLIKLENVI
CCCHHHHCCCCCEEEHHHHHHHHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHH
DTLAMARKIFPGSPASLDALCRRFRISLEQRELHGALLDAQLLARVYIELSKGSQVSMSF
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LEGSSVSSEKKMFKEREKLIITPEESLEHSELIRRLKNPLWDEILMEE
CCCCCCCHHHHHHHHHCCEEECCHHCCCHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]