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The map label for this gene is iscS

Identifier: 88608058

GI number: 88608058

Start: 245003

End: 246211

Strand: Reverse

Name: iscS

Synonym: NSE_0303

Alternate gene names: 88608058

Gene position: 246211-245003 (Counterclockwise)

Preceding gene: 88608399

Following gene: 88608191

Centisome position: 28.66

GC content: 42.76

Gene sequence:

>1209_bases
TTGCCAGTATTCCTTGATAATCAATCCACAACAAAACCTGATCCAGAAGTCATTGAGGCAATGGTCAGTCTCTTTGATCG
TCCTTGCAATCCACATTCGAGAACACATTCATATGGATGGCATGCCGAAGCTGCTGTGGAAAATGCACGTGAAAAAATTG
CACACCTTATTGGTGCTTCTTCTAAAGACATCGTTTTTACATCTGGTGCAACAGAATCAAATAATTTAGCTATCAAGGGG
GTGGCTAATTTTTATCGTTCAAATGAAAAAAATCACATAATTACCCTCTCTACAGAGCATAAATGCGTCCTGGACAGTTG
CAGAAACTTAGAAACAAGTGGAATCGAAGTAACTTACCTTCCAGTTGAGAAAAACGGCATCGTAAATTTGGACGTACTCA
AAAAAGCCATAAAACCATCAACTATGATGGTGTCTATAATGGCTGCAAATAACGAAATAGGTGTCTTGCAACCGCTCAAA
GAGATCGGGCAAATATGTAGGGAAGCGGAGATAATCTTTCACACAGATGCAGCGCAAGCCTTTGGTAAAATCCAGCTCGA
TGTGGAAGAAATGCAGATCTCCCTTATGAGCATTTCTGGTCATAAGATATACGGCCCAATGGGCATTGGAGCACTTTATA
TTCGCAGAAAAAACCCTAGGATAAGGGTGACACCGCTGTTCAGTGGAGGTGGTCAAGAGAGAGGTATTCGCTCCGGTACA
CTTCCTACTCCTTTGATTGTTGGTTTCGGAAAAGCAGCAGAAATTGCAGCAAGGGTCATGGAGGAAGAAAACAAGAGAGT
GAAGTTTTTAGCAAATAAACTGTATGAAATAATCAAGGCAGGAATACCAGATATCGTTCTGAACGGAGATCTCTCAAGAA
GGCTACCTGGCAACCTTAATATCAGTTTCCCTTATGTTGAGGGGGAATCAATAATCATGGCGATCCCAGAGATATCTGTT
AGCTCCGGATCTGCGTGCACATCAGCATCATTAGAGCCATCCTACGTTCTGAAAGCACTTGGAGTTGCAGAAGACCTCGC
CCACTCATCAATACGCTTTTCGGTTGGTAGGTACAACACAGAGGAAGAAATAATCATGGCTGGTGAGTTGCTTGTGAAAC
AAGTCATTCGCCTGCGTGAGATGAGCCCTCTATGGGAAATGGCCCAAGAAGGGATAGATCTTAACTCAATAAAATGGGAT
ACGAGCTAG

Upstream 100 bases:

>100_bases
GAAGAAGTGAAAAAGCTTGTATCAACATGGGAGATATTGTACAACATTAGGGCGTAAAACGATTATTACATAGTTTTAAT
TAGGAAAATGAAAAATGAAG

Downstream 100 bases:

>100_bases
GCAAACGGTTTACAAAATAGGTAATATTGATATTTAGTTTAGGAGGAGGACGTTTATGGCTTATAGTGATTCCGTATTAA
ATCATTACAACAATCCAAGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPVFLDNQSTTKPDPEVIEAMVSLFDRPCNPHSRTHSYGWHAEAAVENAREKIAHLIGASSKDIVFTSGATESNNLAIKG
VANFYRSNEKNHIITLSTEHKCVLDSCRNLETSGIEVTYLPVEKNGIVNLDVLKKAIKPSTMMVSIMAANNEIGVLQPLK
EIGQICREAEIIFHTDAAQAFGKIQLDVEEMQISLMSISGHKIYGPMGIGALYIRRKNPRIRVTPLFSGGGQERGIRSGT
LPTPLIVGFGKAAEIAARVMEEENKRVKFLANKLYEIIKAGIPDIVLNGDLSRRLPGNLNISFPYVEGESIIMAIPEISV
SSGSACTSASLEPSYVLKALGVAEDLAHSSIRFSVGRYNTEEEIIMAGELLVKQVIRLREMSPLWEMAQEGIDLNSIKWD
TS

Sequences:

>Translated_402_residues
MPVFLDNQSTTKPDPEVIEAMVSLFDRPCNPHSRTHSYGWHAEAAVENAREKIAHLIGASSKDIVFTSGATESNNLAIKG
VANFYRSNEKNHIITLSTEHKCVLDSCRNLETSGIEVTYLPVEKNGIVNLDVLKKAIKPSTMMVSIMAANNEIGVLQPLK
EIGQICREAEIIFHTDAAQAFGKIQLDVEEMQISLMSISGHKIYGPMGIGALYIRRKNPRIRVTPLFSGGGQERGIRSGT
LPTPLIVGFGKAAEIAARVMEEENKRVKFLANKLYEIIKAGIPDIVLNGDLSRRLPGNLNISFPYVEGESIIMAIPEISV
SSGSACTSASLEPSYVLKALGVAEDLAHSSIRFSVGRYNTEEEIIMAGELLVKQVIRLREMSPLWEMAQEGIDLNSIKWD
TS
>Mature_401_residues
PVFLDNQSTTKPDPEVIEAMVSLFDRPCNPHSRTHSYGWHAEAAVENAREKIAHLIGASSKDIVFTSGATESNNLAIKGV
ANFYRSNEKNHIITLSTEHKCVLDSCRNLETSGIEVTYLPVEKNGIVNLDVLKKAIKPSTMMVSIMAANNEIGVLQPLKE
IGQICREAEIIFHTDAAQAFGKIQLDVEEMQISLMSISGHKIYGPMGIGALYIRRKNPRIRVTPLFSGGGQERGIRSGTL
PTPLIVGFGKAAEIAARVMEEENKRVKFLANKLYEIIKAGIPDIVLNGDLSRRLPGNLNISFPYVEGESIIMAIPEISVS
SGSACTSASLEPSYVLKALGVAEDLAHSSIRFSVGRYNTEEEIIMAGELLVKQVIRLREMSPLWEMAQEGIDLNSIKWDT
S

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=399, Percent_Identity=61.9047619047619, Blast_Score=526, Evalue=1e-149,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=36.5384615384615, Blast_Score=229, Evalue=5e-60,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=56.4676616915423, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787970, Length=384, Percent_Identity=24.21875, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789175, Length=232, Percent_Identity=27.5862068965517, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=399, Percent_Identity=58.8972431077694, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=34.9544072948328, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=56.8922305764411, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI20129463, Length=399, Percent_Identity=60.1503759398496, Blast_Score=510, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44120; Mature: 43989

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFLDNQSTTKPDPEVIEAMVSLFDRPCNPHSRTHSYGWHAEAAVENAREKIAHLIGAS
CCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
SKDIVFTSGATESNNLAIKGVANFYRSNEKNHIITLSTEHKCVLDSCRNLETSGIEVTYL
CCCEEEECCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEE
PVEKNGIVNLDVLKKAIKPSTMMVSIMAANNEIGVLQPLKEIGQICREAEIIFHTDAAQA
EECCCCEEEHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEECHHHH
FGKIQLDVEEMQISLMSISGHKIYGPMGIGALYIRRKNPRIRVTPLFSGGGQERGIRSGT
HCEEEEEHHHHEEEEEEECCCEEECCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCC
LPTPLIVGFGKAAEIAARVMEEENKRVKFLANKLYEIIKAGIPDIVLNGDLSRRLPGNLN
CCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCE
ISFPYVEGESIIMAIPEISVSSGSACTSASLEPSYVLKALGVAEDLAHSSIRFSVGRYNT
EEEEEECCCEEEEEECCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCC
EEEIIMAGELLVKQVIRLREMSPLWEMAQEGIDLNSIKWDTS
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEECCCC
>Mature Secondary Structure 
PVFLDNQSTTKPDPEVIEAMVSLFDRPCNPHSRTHSYGWHAEAAVENAREKIAHLIGAS
CEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
SKDIVFTSGATESNNLAIKGVANFYRSNEKNHIITLSTEHKCVLDSCRNLETSGIEVTYL
CCCEEEECCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEE
PVEKNGIVNLDVLKKAIKPSTMMVSIMAANNEIGVLQPLKEIGQICREAEIIFHTDAAQA
EECCCCEEEHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEECHHHH
FGKIQLDVEEMQISLMSISGHKIYGPMGIGALYIRRKNPRIRVTPLFSGGGQERGIRSGT
HCEEEEEHHHHEEEEEEECCCEEECCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCC
LPTPLIVGFGKAAEIAARVMEEENKRVKFLANKLYEIIKAGIPDIVLNGDLSRRLPGNLN
CCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCE
ISFPYVEGESIIMAIPEISVSSGSACTSASLEPSYVLKALGVAEDLAHSSIRFSVGRYNT
EEEEEECCCEEEEEECCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCC
EEEIIMAGELLVKQVIRLREMSPLWEMAQEGIDLNSIKWDTS
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA