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The map label for this gene is ispA [H]

Identifier: 88607980

GI number: 88607980

Start: 615713

End: 616483

Strand: Direct

Name: ispA [H]

Synonym: NSE_0694

Alternate gene names: 88607980

Gene position: 615713-616483 (Clockwise)

Preceding gene: 88608177

Following gene: 88608548

Centisome position: 71.68

GC content: 38.78

Gene sequence:

>771_bases
ATGACAGTTAAAAAGAAATTGCAAAAAGCATCAAGATTGATTCGCGAGACTCTAAAAGAGAATCTGTCTCTATTTCCAAG
TGAAGATAAACTTTTTCAAGCAATCAGATATTCCCTCCTTGCAGAGAGCTCACATATTCGTGGATTTTTAGTGTTAGAAC
TTGGCACATTATTCAATATCCCATACGTGGACACTATAGAAGTTGCTGCTGCAATTGAAATGATCCACTCTTTTTCACTC
ATCCATGATGATCTCCCTGCCCTGGATAACTCAGATGAAAGAAGGAATCAGCCTGCATGCCATATTGCTTTCCAAGAGAG
CACCGCCATACTCGCAGGAGATGCCTTGCTAGTTTTAGCTTATCAGATGCTCAGTCGTTATGGCACACCGCTTGTACAAT
GTACTTCCAAATATGTATTAGAAATGATCACAGGACAGGTTCATGACGTCATAGGCAGTTCTACACTCACCGCGGATGAA
ATCAATAAAATGAAATCTGGCTCACTTTTTGCATTGAGTTGTTCTAGCGTTGGTCACCTCGCGAATAGTGGACAAAGTGA
GCTGACTGTATTAGAAGCATTTGGGTACGAAATAGGCATAATTTTTCAAGCTGTGGATGACATAAAAGATGGAGACGCTA
AAAATGACCCTCAAAGGAGTATCACTGTTTCTCTAAAAAAAATTAAGAGAATTATTGATACAACATTTCTGCTTGAAAAA
AAGACACTTATAGACGAACTAATTACCTTAATTATCAACGGATCGCTCTAA

Upstream 100 bases:

>100_bases
ACGAAAGCTGCGTGATCTTGATCATCGACAGTAAGAGATTGCTCTGTTAGATTACCCTATTCTCCACATTTTTTATTCGT
AATGCACACCAAAATCAGAT

Downstream 100 bases:

>100_bases
GAATCCGCAAAGCAAAAGTGCTAACTCATAGAATCCGTTTTTAGGCTAAGTGAGTCATCAGAACATGATCTAACTGACCT
AAAACCAAAAATACTTGATA

Product: putative geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTVKKKLQKASRLIRETLKENLSLFPSEDKLFQAIRYSLLAESSHIRGFLVLELGTLFNIPYVDTIEVAAAIEMIHSFSL
IHDDLPALDNSDERRNQPACHIAFQESTAILAGDALLVLAYQMLSRYGTPLVQCTSKYVLEMITGQVHDVIGSSTLTADE
INKMKSGSLFALSCSSVGHLANSGQSELTVLEAFGYEIGIIFQAVDDIKDGDAKNDPQRSITVSLKKIKRIIDTTFLLEK
KTLIDELITLIINGSL

Sequences:

>Translated_256_residues
MTVKKKLQKASRLIRETLKENLSLFPSEDKLFQAIRYSLLAESSHIRGFLVLELGTLFNIPYVDTIEVAAAIEMIHSFSL
IHDDLPALDNSDERRNQPACHIAFQESTAILAGDALLVLAYQMLSRYGTPLVQCTSKYVLEMITGQVHDVIGSSTLTADE
INKMKSGSLFALSCSSVGHLANSGQSELTVLEAFGYEIGIIFQAVDDIKDGDAKNDPQRSITVSLKKIKRIIDTTFLLEK
KTLIDELITLIINGSL
>Mature_255_residues
TVKKKLQKASRLIRETLKENLSLFPSEDKLFQAIRYSLLAESSHIRGFLVLELGTLFNIPYVDTIEVAAAIEMIHSFSLI
HDDLPALDNSDERRNQPACHIAFQESTAILAGDALLVLAYQMLSRYGTPLVQCTSKYVLEMITGQVHDVIGSSTLTADEI
NKMKSGSLFALSCSSVGHLANSGQSELTVLEAFGYEIGIIFQAVDDIKDGDAKNDPQRSITVSLKKIKRIIDTTFLLEKK
TLIDELITLIINGSL

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=228, Percent_Identity=31.5789473684211, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789578, Length=157, Percent_Identity=29.9363057324841, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17505681, Length=151, Percent_Identity=30.4635761589404, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 28276; Mature: 28145

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKKKLQKASRLIRETLKENLSLFPSEDKLFQAIRYSLLAESSHIRGFLVLELGTLFNI
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCC
PYVDTIEVAAAIEMIHSFSLIHDDLPALDNSDERRNQPACHIAFQESTAILAGDALLVLA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEECCCEEEECHHHHHHH
YQMLSRYGTPLVQCTSKYVLEMITGQVHDVIGSSTLTADEINKMKSGSLFALSCSSVGHL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCHHHHH
ANSGQSELTVLEAFGYEIGIIFQAVDDIKDGDAKNDPQRSITVSLKKIKRIIDTTFLLEK
HCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KTLIDELITLIINGSL
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVKKKLQKASRLIRETLKENLSLFPSEDKLFQAIRYSLLAESSHIRGFLVLELGTLFNI
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCC
PYVDTIEVAAAIEMIHSFSLIHDDLPALDNSDERRNQPACHIAFQESTAILAGDALLVLA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEECCCEEEECHHHHHHH
YQMLSRYGTPLVQCTSKYVLEMITGQVHDVIGSSTLTADEINKMKSGSLFALSCSSVGHL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEECCHHHHH
ANSGQSELTVLEAFGYEIGIIFQAVDDIKDGDAKNDPQRSITVSLKKIKRIIDTTFLLEK
HCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KTLIDELITLIINGSL
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]