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The map label for this gene is thrS [H]

Identifier: 88607718

GI number: 88607718

Start: 1332575

End: 1334422

Strand: Reverse

Name: thrS [H]

Synonym: APH_1264

Alternate gene names: 88607718

Gene position: 1334422-1332575 (Counterclockwise)

Preceding gene: 88607375

Following gene: 88607227

Centisome position: 90.7

GC content: 40.69

Gene sequence:

>1848_bases
GTGGTTTCAACCATATTTTCAGACATTGTAGAGTCTGTAATAGCAGTTTACATAGATGGGGAAGTGTGTGATCTTGCGAG
CACAATAGCTGCCAGTGCCGTCGTTAATCCTGTGCTGATCGATTGTGAAGAAGGAATAAATATCTTACGCAAGAGTACTG
CGTTTATAACCGCCATGGCAGTGAGCTCAGTTGTTCCGAATATGAAGTTGGCTGTATGTACTGAAACGTCAGACGGGTTT
TATTTGGATTTCGATTCTGAGACTAAATTGTCAGAAAAGAGCTTCGAAGACATTGAGAAAAAGATAGAAGAGATCGTACG
CAAGGACGAGAAGTTTGTACGGGAAACTTGGTCAAAGAAGGAAGCCATTACTTTTTTTGAAAATAACTCTGAGACTTATC
AACTAGAAATCCTAGAGCAGAACACTCAAGAGAACATTATTGTTTATCGTTGTGGTAGCCATATTTTCCAGAATCATAGC
CCTTTAGTTCCATCAACTAAATATATAGGAGCTCTAAAGCTTACAAAGTTGTCGGGTGTATATTGGCTTGGAGATAGCAA
AAATAAAATGCTGCAGCGCATATCTGGGGTTGTTCAGGAAAACAAGGAAAAACTTGATGCATATTTCAAACTTTTAGAAG
AGGCAGAAAGAAGAGATCATCGCAAAATAGCGAAGGATTTAGAGTGGTTTCACATACAGAATGAAGCGCTGGGGCAAGTG
TTTTGGCATGATAAGGGATGGACATTGTACCGTACCATAGAGGAATATTTAAGGAAAAAGCTTCGGGAGAACGGATATAC
GGAAATAAAGACGCCCATAATGTTAGATCGCAAGCTTTGGGAGAAGTCAGGGCATTGGGAAAAATTCCGTGAAAACATGT
TTATAGTCAGTGATCAAAACTCGCAAGAGTTAGCCATAAAGCCTATGAACTGTCCTTGCCACGTTCAGATATTTAAATCA
AAAACAAGGAGCTATAAAGATCTGCCTATCCGTATGGCTGAATTTGGAATGTGTCATAGAAACGAGCCTTCTGGTTCTCT
ATATGGATTGATGAGAGTTAGGGGATTTACACAAGATGATGCGCATATTTTCTGCACACATGATCAAATACGTGACGAGG
TAAGAAAATTTTACAAGCTTCTAGTGGAGGTCTACAACGACTTTGGTTTCAAGAACATAGATGTAAAGCTATCTGACCGT
CCTGAGGAGAGAATAGGCAGTGATGAGATGTGGGATATGGCAGAGCGTTCTCTCATGGATTCCATGGATGACGAAGGTGT
TAAATACACTATAAACAAGGGAGATGGCGCATTTTATGGTCCAAAATTGGAATTCACCTTGAAAGACGCTCTAGGAAGAA
ATTGGCAGTGTGGAACCATACAGTTAGACTTTGTACTACCGAGTCGTTTAGGTGCATACTATGTTGGTTCTGATGGAAAT
AAGCACCATCCTGTGATGATCCATAGAGCGATACTGGGAACTTTGGAACGTTTTATAGGGATTTTGATAGAAAGTTATGC
TGGTAATCTTCCGGCTTGGTTGGCCCCGGTACAATTGGAAATTCTAACAGTGAATAGTAACGCTTTAGAATATGCAACTA
CTCTTGCAAACAAGGCACGGGAGCATAATATTCGTGTAGAATTGAATAGTGGAGAGGAGAGTATCGGGCAAAAAATACGT
AAATCCATTTTTAACAAAATTCCTGTATTATGGGTGGTTGGATCGGCGGAGACAGCTGACTGTACTGTGTCTGTGCGTCA
TTACGGTACGAGCAATGCGTGTAGCATGGAGGCTGATAAAGCGTTGAAAACGTTAGTGACTTATGTTAGCATCCGTCAGG
TGCTTTAG

Upstream 100 bases:

>100_bases
GTGTTTCCTTTGAAAGATGCCAAAGATGATAAATATTACCACTTCTTATTATGGAGATGATAGATCATGTAGCTTCTCTG
AACCCATAACTGCGTCGGTA

Downstream 100 bases:

>100_bases
TGTTTAGGTAAGTGGGTTAAAAGTGGATAAAAAAGGTCCTAGAATAAACGATGCTATCACTACGAAGAGGGTTCGTCTCG
TTGACGAGACTGGAACTATG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MVSTIFSDIVESVIAVYIDGEVCDLASTIAASAVVNPVLIDCEEGINILRKSTAFITAMAVSSVVPNMKLAVCTETSDGF
YLDFDSETKLSEKSFEDIEKKIEEIVRKDEKFVRETWSKKEAITFFENNSETYQLEILEQNTQENIIVYRCGSHIFQNHS
PLVPSTKYIGALKLTKLSGVYWLGDSKNKMLQRISGVVQENKEKLDAYFKLLEEAERRDHRKIAKDLEWFHIQNEALGQV
FWHDKGWTLYRTIEEYLRKKLRENGYTEIKTPIMLDRKLWEKSGHWEKFRENMFIVSDQNSQELAIKPMNCPCHVQIFKS
KTRSYKDLPIRMAEFGMCHRNEPSGSLYGLMRVRGFTQDDAHIFCTHDQIRDEVRKFYKLLVEVYNDFGFKNIDVKLSDR
PEERIGSDEMWDMAERSLMDSMDDEGVKYTINKGDGAFYGPKLEFTLKDALGRNWQCGTIQLDFVLPSRLGAYYVGSDGN
KHHPVMIHRAILGTLERFIGILIESYAGNLPAWLAPVQLEILTVNSNALEYATTLANKAREHNIRVELNSGEESIGQKIR
KSIFNKIPVLWVVGSAETADCTVSVRHYGTSNACSMEADKALKTLVTYVSIRQVL

Sequences:

>Translated_615_residues
MVSTIFSDIVESVIAVYIDGEVCDLASTIAASAVVNPVLIDCEEGINILRKSTAFITAMAVSSVVPNMKLAVCTETSDGF
YLDFDSETKLSEKSFEDIEKKIEEIVRKDEKFVRETWSKKEAITFFENNSETYQLEILEQNTQENIIVYRCGSHIFQNHS
PLVPSTKYIGALKLTKLSGVYWLGDSKNKMLQRISGVVQENKEKLDAYFKLLEEAERRDHRKIAKDLEWFHIQNEALGQV
FWHDKGWTLYRTIEEYLRKKLRENGYTEIKTPIMLDRKLWEKSGHWEKFRENMFIVSDQNSQELAIKPMNCPCHVQIFKS
KTRSYKDLPIRMAEFGMCHRNEPSGSLYGLMRVRGFTQDDAHIFCTHDQIRDEVRKFYKLLVEVYNDFGFKNIDVKLSDR
PEERIGSDEMWDMAERSLMDSMDDEGVKYTINKGDGAFYGPKLEFTLKDALGRNWQCGTIQLDFVLPSRLGAYYVGSDGN
KHHPVMIHRAILGTLERFIGILIESYAGNLPAWLAPVQLEILTVNSNALEYATTLANKAREHNIRVELNSGEESIGQKIR
KSIFNKIPVLWVVGSAETADCTVSVRHYGTSNACSMEADKALKTLVTYVSIRQVL
>Mature_615_residues
MVSTIFSDIVESVIAVYIDGEVCDLASTIAASAVVNPVLIDCEEGINILRKSTAFITAMAVSSVVPNMKLAVCTETSDGF
YLDFDSETKLSEKSFEDIEKKIEEIVRKDEKFVRETWSKKEAITFFENNSETYQLEILEQNTQENIIVYRCGSHIFQNHS
PLVPSTKYIGALKLTKLSGVYWLGDSKNKMLQRISGVVQENKEKLDAYFKLLEEAERRDHRKIAKDLEWFHIQNEALGQV
FWHDKGWTLYRTIEEYLRKKLRENGYTEIKTPIMLDRKLWEKSGHWEKFRENMFIVSDQNSQELAIKPMNCPCHVQIFKS
KTRSYKDLPIRMAEFGMCHRNEPSGSLYGLMRVRGFTQDDAHIFCTHDQIRDEVRKFYKLLVEVYNDFGFKNIDVKLSDR
PEERIGSDEMWDMAERSLMDSMDDEGVKYTINKGDGAFYGPKLEFTLKDALGRNWQCGTIQLDFVLPSRLGAYYVGSDGN
KHHPVMIHRAILGTLERFIGILIESYAGNLPAWLAPVQLEILTVNSNALEYATTLANKAREHNIRVELNSGEESIGQKIR
KSIFNKIPVLWVVGSAETADCTVSVRHYGTSNACSMEADKALKTLVTYVSIRQVL

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=590, Percent_Identity=37.1186440677966, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI38202255, Length=592, Percent_Identity=37.3310810810811, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI20070344, Length=632, Percent_Identity=33.8607594936709, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1788013, Length=612, Percent_Identity=46.078431372549, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1786392, Length=160, Percent_Identity=22.5, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=590, Percent_Identity=36.7796610169491, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71984184, Length=590, Percent_Identity=36.7796610169491, Blast_Score=406, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6322112, Length=590, Percent_Identity=37.1186440677966, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322654, Length=378, Percent_Identity=37.3015873015873, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24583842, Length=585, Percent_Identity=36.7521367521368, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28574141, Length=585, Percent_Identity=36.7521367521368, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24583839, Length=585, Percent_Identity=36.7521367521368, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 70498; Mature: 70498

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTIFSDIVESVIAVYIDGEVCDLASTIAASAVVNPVLIDCEEGINILRKSTAFITAMA
CHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHH
VSSVVPNMKLAVCTETSDGFYLDFDSETKLSEKSFEDIEKKIEEIVRKDEKFVRETWSKK
HHHHCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EAITFFENNSETYQLEILEQNTQENIIVYRCGSHIFQNHSPLVPSTKYIGALKLTKLSGV
CEEEEEECCCCEEEEEEEECCCCCCEEEEECCCHHHCCCCCCCCCCHHHCEEEEEEECCE
YWLGDSKNKMLQRISGVVQENKEKLDAYFKLLEEAERRDHRKIAKDLEWFHIQNEALGQV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCEE
FWHDKGWTLYRTIEEYLRKKLRENGYTEIKTPIMLDRKLWEKSGHWEKFRENMFIVSDQN
EEECCCCHHHHHHHHHHHHHHHHCCCCEECCCHHCCHHHHHCCCCHHHHHCCEEEEECCC
SQELAIKPMNCPCHVQIFKSKTRSYKDLPIRMAEFGMCHRNEPSGSLYGLMRVRGFTQDD
CCEEEEEECCCCEEEEEEHHHCCCCCCCCEEHHHCCCCCCCCCCCHHEEEHHHCCCCCCC
AHIFCTHDQIRDEVRKFYKLLVEVYNDFGFKNIDVKLSDRPEERIGSDEMWDMAERSLMD
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHHH
SMDDEGVKYTINKGDGAFYGPKLEFTLKDALGRNWQCGTIQLDFVLPSRLGAYYVGSDGN
CCCCCCCEEEEECCCCCEECCEEEEEEHHHCCCCCCCCEEEEEEEECCCCCEEEECCCCC
KHHPVMIHRAILGTLERFIGILIESYAGNLPAWLAPVQLEILTVNSNALEYATTLANKAR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEEECCCHHHHHHHHHHHHH
EHNIRVELNSGEESIGQKIRKSIFNKIPVLWVVGSAETADCTVSVRHYGTSNACSMEADK
HCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHH
ALKTLVTYVSIRQVL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVSTIFSDIVESVIAVYIDGEVCDLASTIAASAVVNPVLIDCEEGINILRKSTAFITAMA
CHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHH
VSSVVPNMKLAVCTETSDGFYLDFDSETKLSEKSFEDIEKKIEEIVRKDEKFVRETWSKK
HHHHCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EAITFFENNSETYQLEILEQNTQENIIVYRCGSHIFQNHSPLVPSTKYIGALKLTKLSGV
CEEEEEECCCCEEEEEEEECCCCCCEEEEECCCHHHCCCCCCCCCCHHHCEEEEEEECCE
YWLGDSKNKMLQRISGVVQENKEKLDAYFKLLEEAERRDHRKIAKDLEWFHIQNEALGQV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHCEE
FWHDKGWTLYRTIEEYLRKKLRENGYTEIKTPIMLDRKLWEKSGHWEKFRENMFIVSDQN
EEECCCCHHHHHHHHHHHHHHHHCCCCEECCCHHCCHHHHHCCCCHHHHHCCEEEEECCC
SQELAIKPMNCPCHVQIFKSKTRSYKDLPIRMAEFGMCHRNEPSGSLYGLMRVRGFTQDD
CCEEEEEECCCCEEEEEEHHHCCCCCCCCEEHHHCCCCCCCCCCCHHEEEHHHCCCCCCC
AHIFCTHDQIRDEVRKFYKLLVEVYNDFGFKNIDVKLSDRPEERIGSDEMWDMAERSLMD
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHHH
SMDDEGVKYTINKGDGAFYGPKLEFTLKDALGRNWQCGTIQLDFVLPSRLGAYYVGSDGN
CCCCCCCEEEEECCCCCEECCEEEEEEHHHCCCCCCCCEEEEEEEECCCCCEEEECCCCC
KHHPVMIHRAILGTLERFIGILIESYAGNLPAWLAPVQLEILTVNSNALEYATTLANKAR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEEECCCHHHHHHHHHHHHH
EHNIRVELNSGEESIGQKIRKSIFNKIPVLWVVGSAETADCTVSVRHYGTSNACSMEADK
HCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCHHHH
ALKTLVTYVSIRQVL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA