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The map label for this gene is metK [H]

Identifier: 88607550

GI number: 88607550

Start: 7895

End: 9073

Strand: Reverse

Name: metK [H]

Synonym: APH_0007

Alternate gene names: 88607550

Gene position: 9073-7895 (Counterclockwise)

Preceding gene: 88607501

Following gene: NA

Centisome position: 0.62

GC content: 41.14

Gene sequence:

>1179_bases
GTGTTTCATCTAGATAGATACTACAACAGCTATTTTATTACGAGTGAGTCAGTTGCTCATGGGCATCCAGATAAGGTTGC
CGATAGGATATCTGATGATATTCTCGACTACTTTATGTCTATAAATCCTGAGGCTCATGTTGCGGTAGAAGCCCTTGTAA
CAAGGGATAATGTTATCATAGCAGGAGAAGTAAGCGGAGTAATAGTAGAGGAGGAAAAGGTTGACGGAGTAGTTCGTAAG
ACACTGAGAGAAATAGGATACGAAAAGGAAGGATTTCACTGGAGAGAGGTTAAAGTGAGGATCATGCTCCAGGAACAATC
TCATGATATAATGTTGGGAGTGAATCGTGGTCGTAAAAAGAAGGGAGCAGGAGATCAGGGAGTAATGTATGGCTATGCCA
TAAACGAGACAGAATCATACATGCCGTCTCCAATATTTTACTCCCACCGTGTTCTTAAAAATTTGTCAGATGCAGTGAAA
CAAGGAAGCATTACCGGTATAGGACCAGATGCAAAGACAGAAATAACCCTCCAGTATGTTGACCATAAGCCCGTAAAAGT
GTTGAGAGCAGTACTTTCAGTGCAGCATGATGAAAGTAGAACACAACAAGACGTCAGGGATCTTGTCTATCCATACTTCG
TATCATCGATACCAGAAGGATGGATGTGTAAAGAAGAGAATTTTCTAGTCAATCCTACAGGAAGGTTCGTTGTAGGAGGG
CCAGTGGGTGATACAGGAATCACTGGTAGAAAGATAATGGTTGATACCTACGGAGGCCACATACCGCACGGCGGAGGCGC
TTTTTCAGGAAAGGATCCAAGTAAGGTAGATCGTTCCGCAGCGTATATGGCAAGATACATAGCTAAAAATGTAGTACATG
CAGGTCTAGCTAGTGAATGTTTGGTACAGATGTCATATGCAATAGGTGTACCAGCTCCACTGACTTTTGCAATCAATACG
TTTGGAACCAGCGTGGTTGATGTAAAGGAGATAGAAAGGTGTATTATAAGTAATATTGATCTTTCAGTCTCAGGAATATG
TGAAAAGCTGTCGCTATATGAACAAATATATAGCCCTACGTCATGTTATGGACATTTTGGAAGGACTCATGAGAATAGTT
TTTCATGGGAAAAGCTAGACCTGTCTCTAATTCTCAATAGAGAGTTTTCTTGTAATTAA

Upstream 100 bases:

>100_bases
ATGTAGTTTTACCAGCACTTAACTTATTGTAAGGAGTAAAATTTTTATGTATTATCGCATGTCTATTATATTCTATTATC
CTTAATAATCCGTCATAAAA

Downstream 100 bases:

>100_bases
TGTAATATAACTACCCACAGTAAAATAAGCCGATTCACAGGGCTTTTTGTTGTGACGGGATTAAGTATAAAAATAACTAA
ACAATCAGTAGGATATTGAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MFHLDRYYNSYFITSESVAHGHPDKVADRISDDILDYFMSINPEAHVAVEALVTRDNVIIAGEVSGVIVEEEKVDGVVRK
TLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKKKGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVK
QGSITGIGPDAKTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVNPTGRFVVGG
PVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNVVHAGLASECLVQMSYAIGVPAPLTFAINT
FGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPTSCYGHFGRTHENSFSWEKLDLSLILNREFSCN

Sequences:

>Translated_392_residues
MFHLDRYYNSYFITSESVAHGHPDKVADRISDDILDYFMSINPEAHVAVEALVTRDNVIIAGEVSGVIVEEEKVDGVVRK
TLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKKKGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVK
QGSITGIGPDAKTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVNPTGRFVVGG
PVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNVVHAGLASECLVQMSYAIGVPAPLTFAINT
FGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPTSCYGHFGRTHENSFSWEKLDLSLILNREFSCN
>Mature_392_residues
MFHLDRYYNSYFITSESVAHGHPDKVADRISDDILDYFMSINPEAHVAVEALVTRDNVIIAGEVSGVIVEEEKVDGVVRK
TLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKKKGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVK
QGSITGIGPDAKTEITLQYVDHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVNPTGRFVVGG
PVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNVVHAGLASECLVQMSYAIGVPAPLTFAINT
FGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPTSCYGHFGRTHENSFSWEKLDLSLILNREFSCN

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=378, Percent_Identity=43.3862433862434, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI4557737, Length=377, Percent_Identity=43.236074270557, Blast_Score=288, Evalue=6e-78,
Organism=Escherichia coli, GI1789311, Length=382, Percent_Identity=47.1204188481675, Blast_Score=345, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI25145633, Length=379, Percent_Identity=42.4802110817942, Blast_Score=287, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17538494, Length=379, Percent_Identity=41.4248021108179, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17538498, Length=379, Percent_Identity=41.4248021108179, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17551082, Length=379, Percent_Identity=40.3693931398417, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=38.9920424403183, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=41.2121212121212, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=42.1212121212121, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI32565903, Length=297, Percent_Identity=42.7609427609428, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI32565909, Length=186, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323209, Length=380, Percent_Identity=40.2631578947368, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=40.1574803149606, Blast_Score=270, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24580527, Length=379, Percent_Identity=43.0079155672823, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24580525, Length=379, Percent_Identity=43.0079155672823, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI45552159, Length=379, Percent_Identity=43.2717678100264, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24580529, Length=379, Percent_Identity=43.2717678100264, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24580537, Length=379, Percent_Identity=43.2717678100264, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24580533, Length=379, Percent_Identity=43.2717678100264, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24580535, Length=379, Percent_Identity=43.2717678100264, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24580531, Length=379, Percent_Identity=43.2717678100264, Blast_Score=274, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24580541, Length=365, Percent_Identity=42.4657534246575, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=40.1639344262295, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43660; Mature: 43660

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHLDRYYNSYFITSESVAHGHPDKVADRISDDILDYFMSINPEAHVAVEALVTRDNVII
CCCCHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCEEE
AGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKK
EECCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCEEEEECCCCCC
KGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDAKTEITLQYV
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEC
DHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVNPTGRFVVGG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEECCCCCEEECC
PVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNVVHAGLASEC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPT
HHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCH
SCYGHFGRTHENSFSWEKLDLSLILNREFSCN
HHHHCCCCCCCCCCCHHEEEEEEEECCCCCCC
>Mature Secondary Structure
MFHLDRYYNSYFITSESVAHGHPDKVADRISDDILDYFMSINPEAHVAVEALVTRDNVII
CCCCHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCEEE
AGEVSGVIVEEEKVDGVVRKTLREIGYEKEGFHWREVKVRIMLQEQSHDIMLGVNRGRKK
EECCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCEEEEECCCCCC
KGAGDQGVMYGYAINETESYMPSPIFYSHRVLKNLSDAVKQGSITGIGPDAKTEITLQYV
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEEC
DHKPVKVLRAVLSVQHDESRTQQDVRDLVYPYFVSSIPEGWMCKEENFLVNPTGRFVVGG
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEECCCCCEEECC
PVGDTGITGRKIMVDTYGGHIPHGGGAFSGKDPSKVDRSAAYMARYIAKNVVHAGLASEC
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHH
LVQMSYAIGVPAPLTFAINTFGTSVVDVKEIERCIISNIDLSVSGICEKLSLYEQIYSPT
HHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCH
SCYGHFGRTHENSFSWEKLDLSLILNREFSCN
HHHHCCCCCCCCCCCHHEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA