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The map label for this gene is folE [H]

Identifier: 88607486

GI number: 88607486

Start: 864306

End: 864806

Strand: Direct

Name: folE [H]

Synonym: APH_0814

Alternate gene names: 88607486

Gene position: 864306-864806 (Clockwise)

Preceding gene: 88607143

Following gene: 88606788

Centisome position: 58.75

GC content: 41.32

Gene sequence:

>501_bases
ATGGCAGAAGTAACGCAATGTGGTAGAGTTTGTCCAACGATTGAAGAAGCTGAAGAGGCGGTTAGAGTCATAATAAGGTG
GATGGGGGATGATCCTAATAGGGAGGGTTTATTAAAAACGCCTCGTAGGGTCTTGGATTACTATAAAAAGTCTTGTTCTG
GTTATGCTCCTGAGCTTCTCTCTCACTATTCTATTCTTCCCTATGATGGGAGTAGTTCAGAAATGGTGTTGGTGAAGAAC
ATAGGGTTTTCTTCAAACTGCGAACATCATCTTAGTCCTATATGTGGTGTGGCGCATATCGCTTATATGCCAAAAGAGAA
GATACTAGATCCTCATGGTGTTGCTGTACTACTAGAGGCCGAGCATTGGTGCGTTGAATGTAGCAACAGGGATACTGCCT
CTAGAGATGAGAGGTTGAATGTACAAACTAGTCAAATGTTAGGGGTTTTTGAAGAGCGTTGTGATTTGCGCAATGAATTC
TTCTTAAGAATTAAAATGTAA

Upstream 100 bases:

>100_bases
CTAAACAAGTTGGTCTTAGATTACAATCGATCAAGACAGGCGGCAATAACCACGGATCTAATAGAAGTGGTTAGTGGATG
CGAAGCTTTGGGATAGGTAT

Downstream 100 bases:

>100_bases
AGGTCTCGGGAATTTAATGCGAACTCAACTAAAGTCATCGTATTGAAAAAATTCTCTTTCTATGCAAATAAAGCTAATAC
AAAGAGCACAAGTACACCTT

Product: GTP cyclohydrolase I, internal deletion

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MAEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELLSHYSILPYDGSSSEMVLVKN
IGFSSNCEHHLSPICGVAHIAYMPKEKILDPHGVAVLLEAEHWCVECSNRDTASRDERLNVQTSQMLGVFEERCDLRNEF
FLRIKM

Sequences:

>Translated_166_residues
MAEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELLSHYSILPYDGSSSEMVLVKN
IGFSSNCEHHLSPICGVAHIAYMPKEKILDPHGVAVLLEAEHWCVECSNRDTASRDERLNVQTSQMLGVFEERCDLRNEF
FLRIKM
>Mature_165_residues
AEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELLSHYSILPYDGSSSEMVLVKNI
GFSSNCEHHLSPICGVAHIAYMPKEKILDPHGVAVLLEAEHWCVECSNRDTASRDERLNVQTSQMLGVFEERCDLRNEFF
LRIKM

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=181, Percent_Identity=30.939226519337, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4503949, Length=181, Percent_Identity=30.939226519337, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI66932970, Length=100, Percent_Identity=36, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI66932972, Length=96, Percent_Identity=36.4583333333333, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=32.0224719101124, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321706, Length=184, Percent_Identity=30.9782608695652, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24656782, Length=170, Percent_Identity=28.8235294117647, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656796, Length=170, Percent_Identity=28.8235294117647, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24656791, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 18922; Mature: 18791

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELL
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
SHYSILPYDGSSSEMVLVKNIGFSSNCEHHLSPICGVAHIAYMPKEKILDPHGVAVLLEA
HHCEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEC
EHWCVECSNRDTASRDERLNVQTSQMLGVFEERCDLRNEFFLRIKM
CCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
AEVTQCGRVCPTIEEAEEAVRVIIRWMGDDPNREGLLKTPRRVLDYYKKSCSGYAPELL
CCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
SHYSILPYDGSSSEMVLVKNIGFSSNCEHHLSPICGVAHIAYMPKEKILDPHGVAVLLEA
HHCEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEC
EHWCVECSNRDTASRDERLNVQTSQMLGVFEERCDLRNEFFLRIKM
CCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA