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The map label for this gene is ousA [H]

Identifier: 88607466

GI number: 88607466

Start: 951469

End: 951894

Strand: Direct

Name: ousA [H]

Synonym: APH_0895

Alternate gene names: 88607466

Gene position: 951469-951894 (Clockwise)

Preceding gene: 88607156

Following gene: 88606993

Centisome position: 64.67

GC content: 46.24

Gene sequence:

>426_bases
ATGACTGTAGGGTTCGGTGCATTATCTGACGTGATTGGTCGAAAGAAGGTGATGGGTGGCGCATCTATTGCGCTCTTCTT
CCTTGCTATACCTGTGTTCTGGCTATTGAGTCAGGAAAGTTTCTGGTATGTAGGGTTGGGTTACTTTCTGTTTGCGATTC
CGTTTGCTGCTACACTTGGTCCATCCAGTGCTGCGATGTCTGAGCTATTCCCCACAAAAGTGCGTTATACTGGCTTTGGA
ATAGCCCGTAACATATCTTCAGCTCTTGGTGGAGGTATGGCTCCTGTGCTTTGTACATGGCTTATGCAAATTACAGGTTG
GAAGGTTGCACCTGGTTTTTGTGTGATGTTCTGGGCGTTGATTGCATGTATTGCATTGAGTAGGATTACCCCAAAGGACA
TTCATAGAGACTGGGTCAAACAGTAG

Upstream 100 bases:

>100_bases
CATATGCACATAGCTTTCTCATTTTATCTCTCTATCATCTGCAGTAAATCCGCAAATTGGCGAATTTATAGAAATAGTAA
ACGTGATGATCAGTGGGGTG

Downstream 100 bases:

>100_bases
TACTCTACTGCTGGGGTTTCTTACTAAAGTCTAGCAGCCATTGTTAATGGCTGCTTGTTAGTTTCCTTTAGCTAGCTTTT
GCCACAAAAACACATAGCAT

Product: putative transporter, truncation

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MTVGFGALSDVIGRKKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKVRYTGFG
IARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWALIACIALSRITPKDIHRDWVKQ

Sequences:

>Translated_141_residues
MTVGFGALSDVIGRKKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKVRYTGFG
IARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWALIACIALSRITPKDIHRDWVKQ
>Mature_140_residues
TVGFGALSDVIGRKKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLGPSSAAMSELFPTKVRYTGFGI
ARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWALIACIALSRITPKDIHRDWVKQ

Specific function: Involved in uptake and accumulation of various osmoprotectants. Allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=119, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 15348; Mature: 15217

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVGFGALSDVIGRKKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLG
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PSSAAMSELFPTKVRYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWAL
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IACIALSRITPKDIHRDWVKQ
HHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
TVGFGALSDVIGRKKVMGGASIALFFLAIPVFWLLSQESFWYVGLGYFLFAIPFAATLG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
PSSAAMSELFPTKVRYTGFGIARNISSALGGGMAPVLCTWLMQITGWKVAPGFCVMFWAL
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IACIALSRITPKDIHRDWVKQ
HHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8550465 [H]