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The map label for this gene is folC [H]

Identifier: 88607153

GI number: 88607153

Start: 792404

End: 793693

Strand: Reverse

Name: folC [H]

Synonym: APH_0747

Alternate gene names: 88607153

Gene position: 793693-792404 (Counterclockwise)

Preceding gene: 88607269

Following gene: 88607872

Centisome position: 53.95

GC content: 42.17

Gene sequence:

>1290_bases
ATGGTATATATGCCACATTGGCCTAAGGTTTTAGAATTCAAACCTGAAGGTCTTACACTTGATAGAATGAGAGAAATTCT
CTGTAGAATCGGGAATCCGGAAAATAACCTCCCGCCAGTTGTGCACGTTGCAGGTACGAATGGTAAAGGGTCTACCATAG
CGTTTTTATCTAGTATATTGCAGGCAGCCGGGATGCGTGTTCATGTATATACTTCGCCGCATTTGCTAGAATTTAATGAA
AGGATTGTTCTTAACGGGAGTAAAATCTCAGACAATCACTTACATGAGATTTTAGAAGAATGTCGTTCTAAGTGCGATGG
GATGACGGTAACCTTTTTTGAGGCGACAACAGCGGCAGCGCTACTTGCATTCTCACGGGTTCATGCGGACATAGTGTTAT
TAGAAGTAGGTATGGGTGGTAGGCTGGATGCTACAAATGTGGTTATGCCTATCCTGACCATTATTACTTCAATTTCCTAT
GATCACACTTGCTTTTTGGGGGATACTGTAGAACTAATAGCAGGGGAAAAAGCGGGAATTCTTAAGGAAGGGGTGAGTTG
TGTTTTAGCTCCTCAGAAGTATGATGCCGCTCGTAGGACGATAGAATATCATGCGGCTGTAAATCATGCTCCAATATACC
GAGGAGGTATAGAATGGTCATGCACAAAAGATGCTGATGGAATGGTGTTTCGTAGTTCTGAAGAGTATAATTTTCCTCTT
CCAGCGCTTCAAGGTGATCATCAAATAGATAACGCAGGCACAGCAATAGCTGCCTGCAGTGTATTATCAGGAAAGTTTGG
ATATGAAATAAGTCATGAAGACATAGAGGCGGGTTTGGTAAATGCTGTATGGCCTGCTAGGCTCGAAAGGATTGCTTCAG
GAAGCCTCTATAACATGTTGCCAAAGTGCTGGAAATTGTTTTTAGATGGTGCCCACAACGTTGCAGGGGCAGAAGCTTTG
AGAGATTGGATCACCGCTTATATGAAGCATGAGGATGTTTATCTCATAGTTGGAATGACGAAAGATAGAGATAGCAAGGC
TTTTTTGGAGCCTTTAAAGCAGCACATTAGGTTTTTATGTACTGTATGTGTTAAGGCTGAGTATCGTGCTCAGTCTGCCG
AAGATATTTTAAAGTGTGCGCTCGAATTGGATATTCCTGCATCTGCTGAAGAGGATATTAGAGCGTCTATACAAAAGATT
ATTGATATCGGTGATAGGGTGCGTAGCGCCACCATATTGGTTTGTGGTTCATTATTCCTTGCTGGTGATTTATTGAGAGA
GAGTCTATAA

Upstream 100 bases:

>100_bases
TAAGTGTAGTTGATATGCTCAATGCATAGATAGTAGGTTTTACATACATGTTGCTTGTAGAGGCAATAGCTCTGATTTTG
TGATTTTCGGCTATGAGAGT

Downstream 100 bases:

>100_bases
CATTGCTAATAAAGGTGAGAGATAGCAAATATTTTTGTTTATGTGCTCTTTGAATTTTCTACGCTATGCCTGTAAAAGCA
AAGTTATATGTGTAAGTATG

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVYMPHWPKVLEFKPEGLTLDRMREILCRIGNPENNLPPVVHVAGTNGKGSTIAFLSSILQAAGMRVHVYTSPHLLEFNE
RIVLNGSKISDNHLHEILEECRSKCDGMTVTFFEATTAAALLAFSRVHADIVLLEVGMGGRLDATNVVMPILTIITSISY
DHTCFLGDTVELIAGEKAGILKEGVSCVLAPQKYDAARRTIEYHAAVNHAPIYRGGIEWSCTKDADGMVFRSSEEYNFPL
PALQGDHQIDNAGTAIAACSVLSGKFGYEISHEDIEAGLVNAVWPARLERIASGSLYNMLPKCWKLFLDGAHNVAGAEAL
RDWITAYMKHEDVYLIVGMTKDRDSKAFLEPLKQHIRFLCTVCVKAEYRAQSAEDILKCALELDIPASAEEDIRASIQKI
IDIGDRVRSATILVCGSLFLAGDLLRESL

Sequences:

>Translated_429_residues
MVYMPHWPKVLEFKPEGLTLDRMREILCRIGNPENNLPPVVHVAGTNGKGSTIAFLSSILQAAGMRVHVYTSPHLLEFNE
RIVLNGSKISDNHLHEILEECRSKCDGMTVTFFEATTAAALLAFSRVHADIVLLEVGMGGRLDATNVVMPILTIITSISY
DHTCFLGDTVELIAGEKAGILKEGVSCVLAPQKYDAARRTIEYHAAVNHAPIYRGGIEWSCTKDADGMVFRSSEEYNFPL
PALQGDHQIDNAGTAIAACSVLSGKFGYEISHEDIEAGLVNAVWPARLERIASGSLYNMLPKCWKLFLDGAHNVAGAEAL
RDWITAYMKHEDVYLIVGMTKDRDSKAFLEPLKQHIRFLCTVCVKAEYRAQSAEDILKCALELDIPASAEEDIRASIQKI
IDIGDRVRSATILVCGSLFLAGDLLRESL
>Mature_429_residues
MVYMPHWPKVLEFKPEGLTLDRMREILCRIGNPENNLPPVVHVAGTNGKGSTIAFLSSILQAAGMRVHVYTSPHLLEFNE
RIVLNGSKISDNHLHEILEECRSKCDGMTVTFFEATTAAALLAFSRVHADIVLLEVGMGGRLDATNVVMPILTIITSISY
DHTCFLGDTVELIAGEKAGILKEGVSCVLAPQKYDAARRTIEYHAAVNHAPIYRGGIEWSCTKDADGMVFRSSEEYNFPL
PALQGDHQIDNAGTAIAACSVLSGKFGYEISHEDIEAGLVNAVWPARLERIASGSLYNMLPKCWKLFLDGAHNVAGAEAL
RDWITAYMKHEDVYLIVGMTKDRDSKAFLEPLKQHIRFLCTVCVKAEYRAQSAEDILKCALELDIPASAEEDIRASIQKI
IDIGDRVRSATILVCGSLFLAGDLLRESL

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=361, Percent_Identity=32.1329639889197, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI66932990, Length=361, Percent_Identity=32.1329639889197, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1788654, Length=408, Percent_Identity=31.1274509803922, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17553148, Length=437, Percent_Identity=28.1464530892449, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=437, Percent_Identity=28.1464530892449, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=437, Percent_Identity=28.1464530892449, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=426, Percent_Identity=30.5164319248826, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324815, Length=473, Percent_Identity=26.8498942917548, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322718, Length=298, Percent_Identity=24.8322147651007, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641571, Length=304, Percent_Identity=31.25, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24581568, Length=147, Percent_Identity=36.0544217687075, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47217; Mature: 47217

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYMPHWPKVLEFKPEGLTLDRMREILCRIGNPENNLPPVVHVAGTNGKGSTIAFLSSIL
CCCCCCCCHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
QAAGMRVHVYTSPHLLEFNERIVLNGSKISDNHLHEILEECRSKCDGMTVTFFEATTAAA
HHCCCEEEEECCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
LLAFSRVHADIVLLEVGMGGRLDATNVVMPILTIITSISYDHTCFLGDTVELIAGEKAGI
HHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCH
LKEGVSCVLAPQKYDAARRTIEYHAAVNHAPIYRGGIEWSCTKDADGMVFRSSEEYNFPL
HHHCCEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCCCEEECCCCCCCCC
PALQGDHQIDNAGTAIAACSVLSGKFGYEISHEDIEAGLVNAVWPARLERIASGSLYNML
CCCCCCCCCCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
PKCWKLFLDGAHNVAGAEALRDWITAYMKHEDVYLIVGMTKDRDSKAFLEPLKQHIRFLC
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
TVCVKAEYRAQSAEDILKCALELDIPASAEEDIRASIQKIIDIGDRVRSATILVCGSLFL
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDLLRESL
HHHHHHHCC
>Mature Secondary Structure
MVYMPHWPKVLEFKPEGLTLDRMREILCRIGNPENNLPPVVHVAGTNGKGSTIAFLSSIL
CCCCCCCCHHEEECCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
QAAGMRVHVYTSPHLLEFNERIVLNGSKISDNHLHEILEECRSKCDGMTVTFFEATTAAA
HHCCCEEEEECCCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
LLAFSRVHADIVLLEVGMGGRLDATNVVMPILTIITSISYDHTCFLGDTVELIAGEKAGI
HHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCCH
LKEGVSCVLAPQKYDAARRTIEYHAAVNHAPIYRGGIEWSCTKDADGMVFRSSEEYNFPL
HHHCCEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCCCEEECCCCCCCCC
PALQGDHQIDNAGTAIAACSVLSGKFGYEISHEDIEAGLVNAVWPARLERIASGSLYNML
CCCCCCCCCCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
PKCWKLFLDGAHNVAGAEALRDWITAYMKHEDVYLIVGMTKDRDSKAFLEPLKQHIRFLC
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
TVCVKAEYRAQSAEDILKCALELDIPASAEEDIRASIQKIIDIGDRVRSATILVCGSLFL
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGDLLRESL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]