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The map label for this gene is cysS

Identifier: 88606973

GI number: 88606973

Start: 584970

End: 586358

Strand: Direct

Name: cysS

Synonym: APH_0555

Alternate gene names: 88606973

Gene position: 584970-586358 (Clockwise)

Preceding gene: 88607746

Following gene: 88607292

Centisome position: 39.76

GC content: 44.28

Gene sequence:

>1389_bases
ATGAAGCTGTACGATACTTTTAGTGCTGCCAAGAGGGTATTTGACCCTATAGATAGCGCATGCGTGAAAATTTATGCATG
TGGTCCTACGGTTTATGATCTGGCACATATAGGAAATGCGCGCTCAGCTGTTGTTTACGATGTTCTCTTTAGATTATTGA
GGGAGCTGTATCCTGAGGTAATTTACGTTCGTAACATTACGGATGTCGACGATAAGATAATAAACGCTGCTGCAGAAACG
GGACAGAATATAGGTGATTTCACGGAGAGGTATATAAGGTACTTCCATGAGGATATGGATGCGCTTAACTGTTTATCGCC
TACAGTAGAGCCAAGGGCGACTGCCGAAATAGATACTATGCTTCAGCTTATTAGTAGGCTAGTGGAAAGTGGGCACGCTT
ATGTGAAAGGTGGCTCTGTGTATTTCAGTATTTCATCTCATAGGCATTACGGTAGGCTATCCGGTAGGAAGATAGACGAG
ATGATTTCTGGGAATAGGGTTAGCATTGACGCTGAGAAATTGCATCCTGGGGATTTTGTTTTGTGGAAGCCTGCAACTGA
GCAAGACATCAAATTGGGAGCAGCTTGGGAAAGCCCGTGGGGTGGGGGTAGGCCTGGTTGGCATATAGAATGCTCTGCCA
TGAGCTATCGCTATCTTGGTGAAAGTTTTGATATTCACGGGGGAGGAGCTGATTTAATGTTTCCTCATCATGAGAATGAA
CTTGCGCAAAATATGTGTGCGTTTTCTGGTAGTGAATACGCTAGATACTGGGTACATAACGGGTTTCTCACTGTCAATGC
AGGGGAAAAAATGAGCAAATCCCTTGGTAATGTGATTACCGTAAGGGGATTAAGAAATAGCGGTATTGAAGGGGCGGTGA
TACGGTATGTATTCTTATGTACACACTATAGAAAGCCGCTTGACTGGAATGAAAAGGCCATTTTTGACGCTCAGAGCGCT
TTGAGTAAAATGCGTAGGTCTTGCGAGGAATTTACATCTGAAGAGCTGAATTCTGATATAGAAGCAGTTGGGGTGCATAA
TATGCTCCTAGAAGCCTTAAAAGATGATATGAACACGCCTATGGCAATTGCGGCATTGCATGCATTGGTAGGAGAGATAA
ATAAAACTACTGATTTTAAGGAGCGGCTGAAGTTGGCACGGGTGCTCAATCGCAGTGCTAAGTTAATGGGCATTACCGAC
GGTTTTGCAGGGAAATCTGCTGAAGAGGCGGTAGATGTAGATAAGATTCAGGAACTTTTAGAGCGTAGACGGGAAGCGAG
GAATGGTGGCAATTACTCTTTAGCAGATGAAATACGGGACCAGCTACATTCTATGGGCATCGTGATAAAGGATGACAAGG
ATGGTGTTACGCGTTGGTCTAGGGCTTAG

Upstream 100 bases:

>100_bases
TGCTCGCGTTTTTGGGAATTTCTGTCACATCTGTTGTCTTGATTGCTGGTCCGAAGCTCGTAATGGGGATGTTCTCTTTT
ATCTACAAATTTTTTGTAAT

Downstream 100 bases:

>100_bases
TTAAGGATATTTTAGATCTCAATATTGTAGAGATTTCCTTTAGTAAGGGCTTTGGCAATAGCAGTATGGTATTGTGGGGT
TGGGGCTAGTAGTACAAAGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKLYDTFSAAKRVFDPIDSACVKIYACGPTVYDLAHIGNARSAVVYDVLFRLLRELYPEVIYVRNITDVDDKIINAAAET
GQNIGDFTERYIRYFHEDMDALNCLSPTVEPRATAEIDTMLQLISRLVESGHAYVKGGSVYFSISSHRHYGRLSGRKIDE
MISGNRVSIDAEKLHPGDFVLWKPATEQDIKLGAAWESPWGGGRPGWHIECSAMSYRYLGESFDIHGGGADLMFPHHENE
LAQNMCAFSGSEYARYWVHNGFLTVNAGEKMSKSLGNVITVRGLRNSGIEGAVIRYVFLCTHYRKPLDWNEKAIFDAQSA
LSKMRRSCEEFTSEELNSDIEAVGVHNMLLEALKDDMNTPMAIAALHALVGEINKTTDFKERLKLARVLNRSAKLMGITD
GFAGKSAEEAVDVDKIQELLERRREARNGGNYSLADEIRDQLHSMGIVIKDDKDGVTRWSRA

Sequences:

>Translated_462_residues
MKLYDTFSAAKRVFDPIDSACVKIYACGPTVYDLAHIGNARSAVVYDVLFRLLRELYPEVIYVRNITDVDDKIINAAAET
GQNIGDFTERYIRYFHEDMDALNCLSPTVEPRATAEIDTMLQLISRLVESGHAYVKGGSVYFSISSHRHYGRLSGRKIDE
MISGNRVSIDAEKLHPGDFVLWKPATEQDIKLGAAWESPWGGGRPGWHIECSAMSYRYLGESFDIHGGGADLMFPHHENE
LAQNMCAFSGSEYARYWVHNGFLTVNAGEKMSKSLGNVITVRGLRNSGIEGAVIRYVFLCTHYRKPLDWNEKAIFDAQSA
LSKMRRSCEEFTSEELNSDIEAVGVHNMLLEALKDDMNTPMAIAALHALVGEINKTTDFKERLKLARVLNRSAKLMGITD
GFAGKSAEEAVDVDKIQELLERRREARNGGNYSLADEIRDQLHSMGIVIKDDKDGVTRWSRA
>Mature_462_residues
MKLYDTFSAAKRVFDPIDSACVKIYACGPTVYDLAHIGNARSAVVYDVLFRLLRELYPEVIYVRNITDVDDKIINAAAET
GQNIGDFTERYIRYFHEDMDALNCLSPTVEPRATAEIDTMLQLISRLVESGHAYVKGGSVYFSISSHRHYGRLSGRKIDE
MISGNRVSIDAEKLHPGDFVLWKPATEQDIKLGAAWESPWGGGRPGWHIECSAMSYRYLGESFDIHGGGADLMFPHHENE
LAQNMCAFSGSEYARYWVHNGFLTVNAGEKMSKSLGNVITVRGLRNSGIEGAVIRYVFLCTHYRKPLDWNEKAIFDAQSA
LSKMRRSCEEFTSEELNSDIEAVGVHNMLLEALKDDMNTPMAIAALHALVGEINKTTDFKERLKLARVLNRSAKLMGITD
GFAGKSAEEAVDVDKIQELLERRREARNGGNYSLADEIRDQLHSMGIVIKDDKDGVTRWSRA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=391, Percent_Identity=35.5498721227622, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI10835051, Length=318, Percent_Identity=36.1635220125786, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI62240992, Length=318, Percent_Identity=36.1635220125786, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21269877, Length=318, Percent_Identity=36.1635220125786, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI303304987, Length=318, Percent_Identity=36.1635220125786, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786737, Length=473, Percent_Identity=41.2262156448203, Blast_Score=340, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17509797, Length=251, Percent_Identity=33.4661354581673, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17509795, Length=251, Percent_Identity=33.4661354581673, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324082, Length=311, Percent_Identity=32.7974276527331, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922218, Length=481, Percent_Identity=32.2245322245322, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI19922364, Length=238, Percent_Identity=39.9159663865546, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ANAPZ (Q2GKF4)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505147.1
- ProteinModelPortal:   Q2GKF4
- SMR:   Q2GKF4
- STRING:   Q2GKF4
- GeneID:   3930041
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0555
- NMPDR:   fig|212042.5.peg.547
- TIGR:   APH_0555
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   APHA212042:APH_0555-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51808; Mature: 51808

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYDTFSAAKRVFDPIDSACVKIYACGPTVYDLAHIGNARSAVVYDVLFRLLRELYPEV
CCCHHHHHHHHHHHCCHHHHHEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHE
IYVRNITDVDDKIINAAAETGQNIGDFTERYIRYFHEDMDALNCLSPTVEPRATAEIDTM
EEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LQLISRLVESGHAYVKGGSVYFSISSHRHYGRLSGRKIDEMISGNRVSIDAEKLHPGDFV
HHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEHHHCCCCCEE
LWKPATEQDIKLGAAWESPWGGGRPGWHIECSAMSYRYLGESFDIHGGGADLMFPHHENE
EECCCCCCCCEECCCCCCCCCCCCCCCEEEECCHHHHHCCCCCEECCCCCCEECCCCHHH
LAQNMCAFSGSEYARYWVHNGFLTVNAGEKMSKSLGNVITVRGLRNSGIEGAVIRYVFLC
HHHHHHHCCCCHHHHHEEECCEEEEECCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHH
THYRKPLDWNEKAIFDAQSALSKMRRSCEEFTSEELNSDIEAVGVHNMLLEALKDDMNTP
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
MAIAALHALVGEINKTTDFKERLKLARVLNRSAKLMGITDGFAGKSAEEAVDVDKIQELL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHH
ERRREARNGGNYSLADEIRDQLHSMGIVIKDDKDGVTRWSRA
HHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKLYDTFSAAKRVFDPIDSACVKIYACGPTVYDLAHIGNARSAVVYDVLFRLLRELYPEV
CCCHHHHHHHHHHHCCHHHHHEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHE
IYVRNITDVDDKIINAAAETGQNIGDFTERYIRYFHEDMDALNCLSPTVEPRATAEIDTM
EEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LQLISRLVESGHAYVKGGSVYFSISSHRHYGRLSGRKIDEMISGNRVSIDAEKLHPGDFV
HHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEHHHCCCCCEE
LWKPATEQDIKLGAAWESPWGGGRPGWHIECSAMSYRYLGESFDIHGGGADLMFPHHENE
EECCCCCCCCEECCCCCCCCCCCCCCCEEEECCHHHHHCCCCCEECCCCCCEECCCCHHH
LAQNMCAFSGSEYARYWVHNGFLTVNAGEKMSKSLGNVITVRGLRNSGIEGAVIRYVFLC
HHHHHHHCCCCHHHHHEEECCEEEEECCHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHH
THYRKPLDWNEKAIFDAQSALSKMRRSCEEFTSEELNSDIEAVGVHNMLLEALKDDMNTP
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
MAIAALHALVGEINKTTDFKERLKLARVLNRSAKLMGITDGFAGKSAEEAVDVDKIQELL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHH
ERRREARNGGNYSLADEIRDQLHSMGIVIKDDKDGVTRWSRA
HHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA