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The map label for this gene is trmD

Identifier: 88606964

GI number: 88606964

Start: 1335485

End: 1336183

Strand: Reverse

Name: trmD

Synonym: APH_1267

Alternate gene names: 88606964

Gene position: 1336183-1335485 (Counterclockwise)

Preceding gene: 88606853

Following gene: 88607375

Centisome position: 90.82

GC content: 42.92

Gene sequence:

>699_bases
ATGATCTTCAACGTTCTAACAATCTTTCCGCAAATGTTTCCCGGCCCTCTTGGCGTATCTAATCTGGGTTCTGCTCTGAA
AAAAGGGCTGTGGACGTTGAACGTTTTTGATATTAGAGCCTTTGCGAATAATAAACATAATACTGTTGATGATACCCCTT
ATGGAGGGGGGCCAGGCATGCTTCTCAGGGCCGATGTGTTGGGAAGATGCATAGATGAAGTTTTGTCTCTGCATCCGAAT
ACTAAACTTATGTTTACTTCTCCTCGAGGTGTAAGTTTTACTCAAGATATCGCTAGACAAACTATGAATTTTGACAATAT
AACCTTACTCTGCGGGCGCTTTGAAGGTATTGATGAGAGAGTGGTAGATTTTTACAAATTGCAGGAAGTTAGCATAGGAG
ATTATGTTCTCTCTGGTGGTGAGTTAGCTGCAATGGTAATTATCGACACGTGTGTTAGAATGGTGCCAGGAGTTATAGGT
AATGCAGAGAGCTTAAAGCAGGAAAGTATGGAGGGTAGCCTTGAATACCCCCAGTATACTAGGCCAGCTTCCTGGAAGGG
GATGGAGGTTCCAGAGGTCCTTCTTACTGGGAATCATGGAGAAATAGAAAAGTGGAGACGAAATGCTTCTCTTTCGATTA
CTGCTGCAAGACGACCGGACTTGCTAAAAGATAGATATGGTGAAAATGACGTGGAATAG

Upstream 100 bases:

>100_bases
AGAAGGAGATGTTCTCTTTTACTTCAAATATTTTTCCATCCATAGATATGAAAACACGAGAAATGACTATCGTGCCTCCA
GAAATTGTTGGAGTATACAA

Downstream 100 bases:

>100_bases
GAAATGAGTGGGTTACTTGAAAAGTTTAATAATAAACAAATAGAACAGTTACGCCTTGCTACGAACCTAGATATACCTGA
GTTTCGTCCAGGAGACACTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIFNVLTIFPQMFPGPLGVSNLGSALKKGLWTLNVFDIRAFANNKHNTVDDTPYGGGPGMLLRADVLGRCIDEVLSLHPN
TKLMFTSPRGVSFTQDIARQTMNFDNITLLCGRFEGIDERVVDFYKLQEVSIGDYVLSGGELAAMVIIDTCVRMVPGVIG
NAESLKQESMEGSLEYPQYTRPASWKGMEVPEVLLTGNHGEIEKWRRNASLSITAARRPDLLKDRYGENDVE

Sequences:

>Translated_232_residues
MIFNVLTIFPQMFPGPLGVSNLGSALKKGLWTLNVFDIRAFANNKHNTVDDTPYGGGPGMLLRADVLGRCIDEVLSLHPN
TKLMFTSPRGVSFTQDIARQTMNFDNITLLCGRFEGIDERVVDFYKLQEVSIGDYVLSGGELAAMVIIDTCVRMVPGVIG
NAESLKQESMEGSLEYPQYTRPASWKGMEVPEVLLTGNHGEIEKWRRNASLSITAARRPDLLKDRYGENDVE
>Mature_232_residues
MIFNVLTIFPQMFPGPLGVSNLGSALKKGLWTLNVFDIRAFANNKHNTVDDTPYGGGPGMLLRADVLGRCIDEVLSLHPN
TKLMFTSPRGVSFTQDIARQTMNFDNITLLCGRFEGIDERVVDFYKLQEVSIGDYVLSGGELAAMVIIDTCVRMVPGVIG
NAESLKQESMEGSLEYPQYTRPASWKGMEVPEVLLTGNHGEIEKWRRNASLSITAARRPDLLKDRYGENDVE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=226, Percent_Identity=43.3628318584071, Blast_Score=185, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_ANAPZ (Q2GIL5)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505786.1
- PDB:   3KNU
- PDBsum:   3KNU
- ProteinModelPortal:   Q2GIL5
- SMR:   Q2GIL5
- STRING:   Q2GIL5
- GeneID:   3930029
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_1267
- NMPDR:   fig|212042.5.peg.1214
- TIGR:   APH_1267
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q2GIL5
- ProtClustDB:   PRK00026
- BioCyc:   APHA212042:APH_1267-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25761; Mature: 25761

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNVLTIFPQMFPGPLGVSNLGSALKKGLWTLNVFDIRAFANNKHNTVDDTPYGGGPGM
CEEHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
LLRADVLGRCIDEVLSLHPNTKLMFTSPRGVSFTQDIARQTMNFDNITLLCGRFEGIDER
EEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHH
VVDFYKLQEVSIGDYVLSGGELAAMVIIDTCVRMVPGVIGNAESLKQESMEGSLEYPQYT
HHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
RPASWKGMEVPEVLLTGNHGEIEKWRRNASLSITAARRPDLLKDRYGENDVE
CCCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MIFNVLTIFPQMFPGPLGVSNLGSALKKGLWTLNVFDIRAFANNKHNTVDDTPYGGGPGM
CEEHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
LLRADVLGRCIDEVLSLHPNTKLMFTSPRGVSFTQDIARQTMNFDNITLLCGRFEGIDER
EEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHH
VVDFYKLQEVSIGDYVLSGGELAAMVIIDTCVRMVPGVIGNAESLKQESMEGSLEYPQYT
HHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
RPASWKGMEVPEVLLTGNHGEIEKWRRNASLSITAARRPDLLKDRYGENDVE
CCCCCCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA