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The map label for this gene is iscS [H]

Identifier: 88606709

GI number: 88606709

Start: 695567

End: 696805

Strand: Direct

Name: iscS [H]

Synonym: APH_0674

Alternate gene names: 88606709

Gene position: 695567-696805 (Clockwise)

Preceding gene: 88607096

Following gene: 88607576

Centisome position: 47.28

GC content: 43.34

Gene sequence:

>1239_bases
ATGAAAAATAATGAGGATAAGGGAGCGAAGCTTCCTGTATTTTTTGATTATCAGGCAACTACTAAGCTTGACGATAGGGT
TCTTGAGGCGATGATCCCATATTTTCAGCAGTTTTCTAATCCACATTCGCGTAGTCACTGTTATGGTTGGAGAGCGGAGT
CTGCAGTTGAAATTGCCAGGGAGCAGGTTGCTCAGTCGATAGGTGCGGATCCTAAGGAAGTGATTTTTACATCAGGAGCT
ACGGAGTCCAACAATTTAGCAATCAAAGGAGTAGCGCGCTTTTATAAGAATCGAGGTAATCACATAGTCACTGTCAGCAC
TGAGCATAAGTGTGTTTTAGATTCATGTCGTCATCTGGAGACTGAGGGGTTTGAGGTTACATATTTAGGCGTCAAGAAAG
ATGGTCTAATAGATTTGGAAGTTCTAAAAAAGGCTATCAGAGAGGATACTATATTAGTATCAGTTATGATGGTGAATAAT
GAGATAGGTGTTATTCAGCCGATCCGTGAGATAGGGGAGATATGCAAAAGTGAAGGAAGGGACGTATTTTTTCATACTGA
TGCGGCGCAAGCTTTTGGAAAACTACTTATAGATGTTAATGAGATGGGCATAGACCTTTTAAGCATCTCTGGGCACAAGA
TATATGGTCCTATGGGGATAGGAGCATTATACGTACGCAAGCGTAATCCTAGAGTGAGGCTAGTTCCACTATTAAGTGGT
GGTGGTCAAGAGCGGGGAATGCGTTCTGGTACAGTTCCTACTCCTCTAGCTGTAGGCCTTGGTGAAGCAGCGAGAATCGC
ATTAGAAGAGATGGATTCTGAGTCTGAGAGGATAAAGAAGTTGCGCGATGTGTTATATGAGAGGATAATGAATGGCTTAC
CTCACGTTGTCTTGAATGGAAGTTATTCTAGCAGGATAGCCGGTAATTTAAATCTAAGCTTTTCGTATGTTGAGGGAGAG
TCGCTGATAATGTCTGTCAGTGATTTGGCTGTGAGTTCGGGTTCTGCATGCACGTCGGCTTCTCTTGAGTCGTCGTATGT
ACTGCGTGCGTTAAATGTAGAGCAAGATCTAGAACACTCTTCCATTAGGTTTGGTATAGGAAGGTTTACCACGTTGGAAG
ATGTAGAGCGTGCGGCTGATCTTATCATCAGCAGTGTACGCAGGCTGAGGGATATTAGTCCTTTATGGGAGATGGTGCAG
GAAGGTACTAACCTGAGTACGATTAAGTGGGATGTATAG

Upstream 100 bases:

>100_bases
AGCAGTGATCAAGTAAATAAAATTATCGAATGTTGGTATAAAATCTACAGTAATTTTCACTCGCTGAGTTGAGTTGTCCT
GTAGGCAGTTGAGGTGGACT

Downstream 100 bases:

>100_bases
TAAGTAGAGGTTTTTATGAGTTATAGTGATGCTGTATTAAATCGCTGCAAGGCTCCTCAGAATGTGGGTTCGCTACCTAA
GGATGATATTTCTGTTGGTA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKNNEDKGAKLPVFFDYQATTKLDDRVLEAMIPYFQQFSNPHSRSHCYGWRAESAVEIAREQVAQSIGADPKEVIFTSGA
TESNNLAIKGVARFYKNRGNHIVTVSTEHKCVLDSCRHLETEGFEVTYLGVKKDGLIDLEVLKKAIREDTILVSVMMVNN
EIGVIQPIREIGEICKSEGRDVFFHTDAAQAFGKLLIDVNEMGIDLLSISGHKIYGPMGIGALYVRKRNPRVRLVPLLSG
GGQERGMRSGTVPTPLAVGLGEAARIALEEMDSESERIKKLRDVLYERIMNGLPHVVLNGSYSSRIAGNLNLSFSYVEGE
SLIMSVSDLAVSSGSACTSASLESSYVLRALNVEQDLEHSSIRFGIGRFTTLEDVERAADLIISSVRRLRDISPLWEMVQ
EGTNLSTIKWDV

Sequences:

>Translated_412_residues
MKNNEDKGAKLPVFFDYQATTKLDDRVLEAMIPYFQQFSNPHSRSHCYGWRAESAVEIAREQVAQSIGADPKEVIFTSGA
TESNNLAIKGVARFYKNRGNHIVTVSTEHKCVLDSCRHLETEGFEVTYLGVKKDGLIDLEVLKKAIREDTILVSVMMVNN
EIGVIQPIREIGEICKSEGRDVFFHTDAAQAFGKLLIDVNEMGIDLLSISGHKIYGPMGIGALYVRKRNPRVRLVPLLSG
GGQERGMRSGTVPTPLAVGLGEAARIALEEMDSESERIKKLRDVLYERIMNGLPHVVLNGSYSSRIAGNLNLSFSYVEGE
SLIMSVSDLAVSSGSACTSASLESSYVLRALNVEQDLEHSSIRFGIGRFTTLEDVERAADLIISSVRRLRDISPLWEMVQ
EGTNLSTIKWDV
>Mature_412_residues
MKNNEDKGAKLPVFFDYQATTKLDDRVLEAMIPYFQQFSNPHSRSHCYGWRAESAVEIAREQVAQSIGADPKEVIFTSGA
TESNNLAIKGVARFYKNRGNHIVTVSTEHKCVLDSCRHLETEGFEVTYLGVKKDGLIDLEVLKKAIREDTILVSVMMVNN
EIGVIQPIREIGEICKSEGRDVFFHTDAAQAFGKLLIDVNEMGIDLLSISGHKIYGPMGIGALYVRKRNPRVRLVPLLSG
GGQERGMRSGTVPTPLAVGLGEAARIALEEMDSESERIKKLRDVLYERIMNGLPHVVLNGSYSSRIAGNLNLSFSYVEGE
SLIMSVSDLAVSSGSACTSASLESSYVLRALNVEQDLEHSSIRFGIGRFTTLEDVERAADLIISSVRRLRDISPLWEMVQ
EGTNLSTIKWDV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=65, Blast_Score=543, Evalue=1e-154,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=34.2995169082126, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=58.4158415841584, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1787970, Length=236, Percent_Identity=27.9661016949153, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789175, Length=236, Percent_Identity=30.5084745762712, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=412, Percent_Identity=61.4077669902913, Blast_Score=526, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=32.8173374613003, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=400, Percent_Identity=60.75, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI20129463, Length=411, Percent_Identity=64.720194647202, Blast_Score=549, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45588; Mature: 45588

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNEDKGAKLPVFFDYQATTKLDDRVLEAMIPYFQQFSNPHSRSHCYGWRAESAVEIAR
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
EQVAQSIGADPKEVIFTSGATESNNLAIKGVARFYKNRGNHIVTVSTEHKCVLDSCRHLE
HHHHHHHCCCHHHEEEECCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCC
TEGFEVTYLGVKKDGLIDLEVLKKAIREDTILVSVMMVNNEIGVIQPIREIGEICKSEGR
CCCEEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
DVFFHTDAAQAFGKLLIDVNEMGIDLLSISGHKIYGPMGIGALYVRKRNPRVRLVPLLSG
EEEEECHHHHHHHHHEEEEHHCCEEEEEECCCEEECCCCCCEEEEEECCCCEEEEEEECC
GGQERGMRSGTVPTPLAVGLGEAARIALEEMDSESERIKKLRDVLYERIMNGLPHVVLNG
CCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
SYSSRIAGNLNLSFSYVEGESLIMSVSDLAVSSGSACTSASLESSYVLRALNVEQDLEHS
CCCCEEEECCEEEEEEECCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHEEECCHHCCCCC
SIRFGIGRFTTLEDVERAADLIISSVRRLRDISPLWEMVQEGTNLSTIKWDV
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MKNNEDKGAKLPVFFDYQATTKLDDRVLEAMIPYFQQFSNPHSRSHCYGWRAESAVEIAR
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
EQVAQSIGADPKEVIFTSGATESNNLAIKGVARFYKNRGNHIVTVSTEHKCVLDSCRHLE
HHHHHHHCCCHHHEEEECCCCCCCCEEHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCC
TEGFEVTYLGVKKDGLIDLEVLKKAIREDTILVSVMMVNNEIGVIQPIREIGEICKSEGR
CCCEEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
DVFFHTDAAQAFGKLLIDVNEMGIDLLSISGHKIYGPMGIGALYVRKRNPRVRLVPLLSG
EEEEECHHHHHHHHHEEEEHHCCEEEEEECCCEEECCCCCCEEEEEECCCCEEEEEEECC
GGQERGMRSGTVPTPLAVGLGEAARIALEEMDSESERIKKLRDVLYERIMNGLPHVVLNG
CCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
SYSSRIAGNLNLSFSYVEGESLIMSVSDLAVSSGSACTSASLESSYVLRALNVEQDLEHS
CCCCEEEECCEEEEEEECCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHEEECCHHCCCCC
SIRFGIGRFTTLEDVERAADLIISSVRRLRDISPLWEMVQEGTNLSTIKWDV
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA