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The map label for this gene is nifS1 [H]

Identifier: 88607096

GI number: 88607096

Start: 694078

End: 695538

Strand: Direct

Name: nifS1 [H]

Synonym: APH_0673

Alternate gene names: 88607096

Gene position: 694078-695538 (Clockwise)

Preceding gene: 88607784

Following gene: 88606709

Centisome position: 47.18

GC content: 43.12

Gene sequence:

>1461_bases
GTGATAGCGGATTCGCAATCGCTCTCTGAAGGATACTTAGAGCGCGTCATTTCACATCTTAAAAGCAGGGGATTAGTAAA
ACCCACTAGGGGGCCTGGAGGGGGGTACTCTTTAGGCATGTCACCAGATCGCATAACCTTGGATATGCTATTAGATTCCG
TGGGAGATAAAGTTAAAATGGTCAGATGTGACGCAGACGGGAATACCGGGTGTATATCTTCCTATGCGGGGAAGTGTAAT
AGTCACGACCTGTGGGAAAGTATGGAAAAGTACATGATGCACTACTTTGCCAATACTACAATACTCGATGTATCTAATAA
TACGCTTAAAGAACTAGGTGGTGGTGGAGATTATATTTACGCCGATGATAATGCGTCTTCAGAAGTGTGCCAGCAAGTTC
GGCATAAGTTAGGTAATGCGCTATTGTTTGGTAATTTTTATAATCCTTCAGCGGTTTATGGCTTAGGACAGAAGGCCAGA
AGTTTAGTAGAAGACGTGAGGCGAGTTGTTATTGAAGCACTTGATGCCAGAGGTTATGAAGTGGTTTTTACTTCTTCTGG
AACGGAAGCCAATAATCTCGTGTTCAATAGTGTAAATGGCAGGCATATAATTTCAGCAATAGAGCATCCGTCAGTGATGA
ATGCAGCAGTGGATCCGGTGTTGATATCGGTAACAGGAGATGGTGTTGTTTCTTTAGAAGCGCTGGAGGAAGCTTTAAAG
TCGGGCGATAGCAAAGGAAGCCTTGTATCTGTTATGTTAGCTAACAACGAGATTGGTGTAATTCAGCCTCTAAAAGACGT
TGTGAATATTGCTCATAAATATGGTGCTGTAGTGCATATGGATGCCGTGCAGGCGTGTGGGAAGATACCTGTAAGTGTGT
TGGATATTGGTGCAGATTTTGTAACCATTTCTTCCCATAAAGTCGGGGGAATTGTGGGTGCTGGTGCTGTGCTTTACAAC
GGTAAAAAGGTCAATGTAGTGCCGATGTTACGTGGTGGCATGCAGGAAAAAGGGTTACGTGCTGGTACTGAGAATATTCC
GGCAATTTATTCTTTGGCTATAGCCTTGGAAAATCTCAAAGATCGTCTGCAAAAGATGTCTAATGTGGCGAGTATGCGTG
ATTTGCTTGAAAAGAAGATTAAGGAAATGGTTCCAGAATGCATAATCTGGGGTTCCGGAGCCGACAGGTTACCTAACACG
ACGTGTATATCAATGCCTGGTGTGAGCAGTGAAACACAACTTATAGGGTTTGATAGCAATGGGATAGCGGTTGGTGTAGG
AGCAGCGTGCAGTAGCGGGAAAATAGGAAGGTCAAAGGTATTAGAAGCACTAGGAGCAAGTGAAGAGGATGCTAAGAATG
CTATTAGGATCAGCTTAGGTCCTGAAGTAAGCAGTGATCAAGTAAATAAAATTATCGAATGTTGGTATAAAATCTACAGT
AATTTTCACTCGCTGAGTTGA

Upstream 100 bases:

>100_bases
TGTCGTGTTGATGACAACGCGTTTGCGTTATGCCGTTATGTTCATGGTTGGTCTAGTGCAAAAGGAGTGTTCTGGTTACG
GCAAACCGAAACGTGCATCG

Downstream 100 bases:

>100_bases
GTTGTCCTGTAGGCAGTTGAGGTGGACTATGAAAAATAATGAGGATAAGGGAGCGAAGCTTCCTGTATTTTTTGATTATC
AGGCAACTACTAAGCTTGAC

Product: rrf2 family protein /cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MIADSQSLSEGYLERVISHLKSRGLVKPTRGPGGGYSLGMSPDRITLDMLLDSVGDKVKMVRCDADGNTGCISSYAGKCN
SHDLWESMEKYMMHYFANTTILDVSNNTLKELGGGGDYIYADDNASSEVCQQVRHKLGNALLFGNFYNPSAVYGLGQKAR
SLVEDVRRVVIEALDARGYEVVFTSSGTEANNLVFNSVNGRHIISAIEHPSVMNAAVDPVLISVTGDGVVSLEALEEALK
SGDSKGSLVSVMLANNEIGVIQPLKDVVNIAHKYGAVVHMDAVQACGKIPVSVLDIGADFVTISSHKVGGIVGAGAVLYN
GKKVNVVPMLRGGMQEKGLRAGTENIPAIYSLAIALENLKDRLQKMSNVASMRDLLEKKIKEMVPECIIWGSGADRLPNT
TCISMPGVSSETQLIGFDSNGIAVGVGAACSSGKIGRSKVLEALGASEEDAKNAIRISLGPEVSSDQVNKIIECWYKIYS
NFHSLS

Sequences:

>Translated_486_residues
MIADSQSLSEGYLERVISHLKSRGLVKPTRGPGGGYSLGMSPDRITLDMLLDSVGDKVKMVRCDADGNTGCISSYAGKCN
SHDLWESMEKYMMHYFANTTILDVSNNTLKELGGGGDYIYADDNASSEVCQQVRHKLGNALLFGNFYNPSAVYGLGQKAR
SLVEDVRRVVIEALDARGYEVVFTSSGTEANNLVFNSVNGRHIISAIEHPSVMNAAVDPVLISVTGDGVVSLEALEEALK
SGDSKGSLVSVMLANNEIGVIQPLKDVVNIAHKYGAVVHMDAVQACGKIPVSVLDIGADFVTISSHKVGGIVGAGAVLYN
GKKVNVVPMLRGGMQEKGLRAGTENIPAIYSLAIALENLKDRLQKMSNVASMRDLLEKKIKEMVPECIIWGSGADRLPNT
TCISMPGVSSETQLIGFDSNGIAVGVGAACSSGKIGRSKVLEALGASEEDAKNAIRISLGPEVSSDQVNKIIECWYKIYS
NFHSLS
>Mature_486_residues
MIADSQSLSEGYLERVISHLKSRGLVKPTRGPGGGYSLGMSPDRITLDMLLDSVGDKVKMVRCDADGNTGCISSYAGKCN
SHDLWESMEKYMMHYFANTTILDVSNNTLKELGGGGDYIYADDNASSEVCQQVRHKLGNALLFGNFYNPSAVYGLGQKAR
SLVEDVRRVVIEALDARGYEVVFTSSGTEANNLVFNSVNGRHIISAIEHPSVMNAAVDPVLISVTGDGVVSLEALEEALK
SGDSKGSLVSVMLANNEIGVIQPLKDVVNIAHKYGAVVHMDAVQACGKIPVSVLDIGADFVTISSHKVGGIVGAGAVLYN
GKKVNVVPMLRGGMQEKGLRAGTENIPAIYSLAIALENLKDRLQKMSNVASMRDLLEKKIKEMVPECIIWGSGADRLPNT
TCISMPGVSSETQLIGFDSNGIAVGVGAACSSGKIGRSKVLEALGASEEDAKNAIRISLGPEVSSDQVNKIIECWYKIYS
NFHSLS

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=373, Percent_Identity=29.4906166219839, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI156713448, Length=402, Percent_Identity=30.5970149253731, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=29.7927461139896, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787970, Length=350, Percent_Identity=27.4285714285714, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=392, Percent_Identity=30.8673469387755, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=32.0987654320988, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=32.4607329842932, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI20129463, Length=374, Percent_Identity=31.0160427807487, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 51798; Mature: 51798

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIADSQSLSEGYLERVISHLKSRGLVKPTRGPGGGYSLGMSPDRITLDMLLDSVGDKVKM
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
VRCDADGNTGCISSYAGKCNSHDLWESMEKYMMHYFANTTILDVSNNTLKELGGGGDYIY
EEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCEEE
ADDNASSEVCQQVRHKLGNALLFGNFYNPSAVYGLGQKARSLVEDVRRVVIEALDARGYE
ECCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEE
VVFTSSGTEANNLVFNSVNGRHIISAIEHPSVMNAAVDPVLISVTGDGVVSLEALEEALK
EEEECCCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCCCEEEEECCCCEEEHHHHHHHHH
SGDSKGSLVSVMLANNEIGVIQPLKDVVNIAHKYGAVVHMDAVQACGKIPVSVLDIGADF
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCEEEEECCCCE
VTISSHKVGGIVGAGAVLYNGKKVNVVPMLRGGMQEKGLRAGTENIPAIYSLAIALENLK
EEECCCCCCCEEECCEEEECCCEEEEEHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
DRLQKMSNVASMRDLLEKKIKEMVPECIIWGSGADRLPNTTCISMPGVSSETQLIGFDSN
HHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCEEEECCCCCCCCEEEEECCC
GIAVGVGAACSSGKIGRSKVLEALGASEEDAKNAIRISLGPEVSSDQVNKIIECWYKIYS
CEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
NFHSLS
HHHCCC
>Mature Secondary Structure
MIADSQSLSEGYLERVISHLKSRGLVKPTRGPGGGYSLGMSPDRITLDMLLDSVGDKVKM
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
VRCDADGNTGCISSYAGKCNSHDLWESMEKYMMHYFANTTILDVSNNTLKELGGGGDYIY
EEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCCCEEE
ADDNASSEVCQQVRHKLGNALLFGNFYNPSAVYGLGQKARSLVEDVRRVVIEALDARGYE
ECCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEE
VVFTSSGTEANNLVFNSVNGRHIISAIEHPSVMNAAVDPVLISVTGDGVVSLEALEEALK
EEEECCCCCCCCEEEECCCCCHHHHHHCCCHHHHHCCCCEEEEECCCCEEEHHHHHHHHH
SGDSKGSLVSVMLANNEIGVIQPLKDVVNIAHKYGAVVHMDAVQACGKIPVSVLDIGADF
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHCCCCCEEEEECCCCE
VTISSHKVGGIVGAGAVLYNGKKVNVVPMLRGGMQEKGLRAGTENIPAIYSLAIALENLK
EEECCCCCCCEEECCEEEECCCEEEEEHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
DRLQKMSNVASMRDLLEKKIKEMVPECIIWGSGADRLPNTTCISMPGVSSETQLIGFDSN
HHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCCEEEECCCCCCCCEEEEECCC
GIAVGVGAACSSGKIGRSKVLEALGASEEDAKNAIRISLGPEVSSDQVNKIIECWYKIYS
CEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
NFHSLS
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]