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The map label for this gene is topB [H]

Identifier: 88196165

GI number: 88196165

Start: 2320524

End: 2322659

Strand: Reverse

Name: topB [H]

Synonym: SAOUHSC_02517

Alternate gene names: 88196165

Gene position: 2322659-2320524 (Counterclockwise)

Preceding gene: 88196166

Following gene: 88196164

Centisome position: 82.32

GC content: 33.05

Gene sequence:

>2136_bases
ATGAAATCTTTAATATTAGCTGAAAAACCATCAGTCGCAAGAGATATTGCTGATGCTTTACAAATAAATCAGAAGCGTAA
TGGTTACTTTGAAAATAACCAATATATTGTCACGTGGGCGTTAGGTCATCTAGTGACAAATGCGACACCTGAACAATACG
ATAAAAATTTAAAGGAATGGCGATTAGAAGACCTTCCAATTATACCTAAATATATGAAAACTGTTGTTATTGGTAAAACA
AGCAAACAATTTAAAACAGTAAAAGCGTTAATTTTAGATAATAAAGTGAAAGATATTATTATTGCAACAGATGCTGGACG
AGAAGGTGAACTAGTTGCAAGATTGATTTTGGATAAAGTTGGTAACAAAAAGCCAATCCGTCGATTATGGATTAGCTCAG
TTACTAAAAAAGCTATTCAACAAGGTTTTAAAAATTTAAAAGACGGTCGTCAATATAACGATTTGTATTATGCAGCGTTA
GCGAGAAGTGAGGCAGATTGGATTGTTGGGATTAATGCAACGCGTGCACTAACAACAAAGTATGATGCACAGCTATCCCT
GGGACGTGTTCAGACACCAACGATTCAATTAGTAAATACACGACAACAAGAGATTAATCAGTTCAAACCACAACAATACT
TTACATTATCATTAACGGTAAAAGGGTTTGATTTTCAGCTAGAATCAAATCAGCGATATACCAATAAAGAAACTTTAGAA
CAGATGGTTAATAATTTGAAAAATGTCGATGGTAAGATTAAATCTGTTGCTACTAAACATAAGAAGTCGTATCCGCAATC
ACTGTACAATTTAACAGATTTACAACAAGATATGTATAGACGTTATAAAATTGGACCTAAAGAAACATTGAATACACTTC
AAAGCTTATATGAGAGACATAAAGTCGTAACCTATCCAAGAACAGATTCAAACTATTTAACAACTGATATGGTAGATACT
ATGAAAGAACGTATTCAGGTGACGATGGCAACAACATATAAAGACCAAGCACGCCCATTAATGTCTAAAACATTTTCATC
AAAAATGTCGATATTTAATAATCAAAAAGTATCTGATCACCATGCAATTATTCCTACAGAAGTGAGACCTGTCATGTCAG
ACTTAAGTAATAGAGAATTAAAGTTATACGATATGATTGTCGAGCGTTTTTTAGAAGCTTTAATGCCTCCGCACGAGTAT
GACGCGATAACTGTAACTTTAGAGGTTGCAGGGCACACATTTGTTTTGAAAGAGAATGTAACAACTGTTTTAGGTTTTAA
ATCTATTAGACAAGGTGAATCTATTACAGAGATGCAACAGCCTTTTTCAGAAGGCGATGAAGTGAAGATTTCAAAAACAA
ATATTAGAGAACATGAAACAACACCTCCAGAATATTTTAATGAAGGTTCGTTATTAAAAGCGATGGAGAACCCTCAGAAC
TTTATTCAATTGAAGGATAAAAAATATGCGCAAACTTTAAAACAAACAGGTGGTATCGGCACAGTTGCAACAAGGGCCGA
CATTATCGATAAATTATTTAATATGAATGCCATTGAATCAAGAGACGGTAAAATTAAAGTAACGTCAAAAGGTAAACAAA
TATTAGAATTAGCACCAGAAGAATTAACGTCGCCACTTTTAACTGCACAATGGGAAGAAAAATTACTTTTAATTGAACGT
GGTAAATATCAGGCGAAAACATTTATTAATGAAATGAAAGATTTTACGAAAGATGTTGTAAATGGGATTAAAAATAGTGA
TCGTAAATATAAACACGATAATTTAACAACCACAGAATGCCCAACGTGTGGTAAATTCATGATTAAAGTTAAAACTAAAA
ATGGTCAGATGCTTGTGTGCCAAGATCCATCTTGTAAGACGAAAAAGAATGTACAGCGCAAAACAAATGCAAGATGTCCA
AACTGTAAAAAGAAATTAACGTTGTTTGGTAAAGGGAAAGAAGCGGTATATCGTTGTGTTTGTGGACATTCTGAAACGCA
AGCACATATGGATCAGCGTATGAAGTCTAAATCCTCTGGTAAAGTATCTCGTAAAGAAATGAAAAAGTATATGAATAAAA
ATGAAGGTTTAGACAATAATCCGTTTAAAGATGCATTAAAGAACTTGAATTTATAG

Upstream 100 bases:

>100_bases
ACATTTAACACATCATTTGCAATCATTGAATTTAAATCAAAGATTAGTGGAATAGATGAAGCACGTTCGAAATAAAAGAA
CGTATGAGAAAGGATAATTT

Downstream 100 bases:

>100_bases
ATAAAATCGAACAAAGTTGAATCAGAAAAACGAAAAGTTCGCTTTTGGTATTGTTTTTTATTAAGAATGATATTAAACTA
TTAAGGTATTTTAAAAAAAG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKT
SKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDT
MKERIQVTMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEY
DAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIER
GKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCP
NCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

Sequences:

>Translated_711_residues
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKT
SKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDT
MKERIQVTMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEY
DAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIER
GKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCP
NCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL
>Mature_711_residues
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKT
SKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDT
MKERIQVTMATTYKDQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEY
DAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIER
GKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCP
NCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=675, Percent_Identity=23.5555555555556, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI10835218, Length=691, Percent_Identity=22.4312590448625, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1788061, Length=600, Percent_Identity=29.3333333333333, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787529, Length=599, Percent_Identity=26.7111853088481, Blast_Score=149, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI32563869, Length=685, Percent_Identity=23.7956204379562, Blast_Score=130, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=615, Percent_Identity=21.7886178861789, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323263, Length=480, Percent_Identity=22.7083333333333, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640096, Length=709, Percent_Identity=23.4132581100141, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24585251, Length=618, Percent_Identity=23.9482200647249, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81553; Mature: 81553

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00396 TOPOISOMERASE_I_PROK ; PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEW
CCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCHHHHHCCHHHH
RLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKV
CCCCCCCCHHHHHHEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHC
GNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCHHEEEEC
YDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
CCCCEECCCCCCCEEEEECCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCHHHHH
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
KVVTYPRTDSNYLTTDMVDTMKERIQVTMATTYKDQARPLMSKTFSSKMSIFNNQKVSDH
CEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
HAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEECCC
TTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
HHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHCCCHH
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPE
HHEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHCCCH
ELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTEC
HHCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCV
CCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHH
CGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHLVTNATPEQYDKNLKEW
CCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHCCCHHHHHCCHHHH
RLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIATDAGREGELVARLILDKV
CCCCCCCCHHHHHHEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHC
GNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAALARSEADWIVGINATRALTTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCHHEEEEC
YDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSLTVKGFDFQLESNQRYTNKETLE
CCCCEECCCCCCCEEEEECCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCHHHHH
QMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQDMYRRYKIGPKETLNTLQSLYERH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
KVVTYPRTDSNYLTTDMVDTMKERIQVTMATTYKDQARPLMSKTFSSKMSIFNNQKVSDH
CEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCC
HAIIPTEVRPVMSDLSNRELKLYDMIVERFLEALMPPHEYDAITVTLEVAGHTFVLKENV
CCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEECCC
TTVLGFKSIRQGESITEMQQPFSEGDEVKISKTNIREHETTPPEYFNEGSLLKAMENPQN
HHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHCCCHH
FIQLKDKKYAQTLKQTGGIGTVATRADIIDKLFNMNAIESRDGKIKVTSKGKQILELAPE
HHEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHCCCH
ELTSPLLTAQWEEKLLLIERGKYQAKTFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTEC
HHCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PTCGKFMIKVKTKNGQMLVCQDPSCKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCV
CCCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHH
CGHSETQAHMDQRMKSKSSGKVSRKEMKKYMNKNEGLDNNPFKDALKNLNL
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA