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The map label for this gene is metH [H]

Identifier: 87201219

GI number: 87201219

Start: 3424461

End: 3425537

Strand: Reverse

Name: metH [H]

Synonym: Saro_3207

Alternate gene names: 87201219

Gene position: 3425537-3424461 (Counterclockwise)

Preceding gene: 87201220

Following gene: 87201218

Centisome position: 96.18

GC content: 62.95

Gene sequence:

>1077_bases
GTGACCCTGATCCCTTCCCCCTTGATTCCTTCAGGCGCCCGCACCGCCTTTCTTGAACAGGCGAGCCAGCGCATCCTCAT
CACCGATGGCGCGTTCGGAACCGAGATCCAGAACTTCAAGCTCTCGGAAGAAGACTATGCCGGTACCCTTGGTCTTGCGA
AGGACCAGAAGGGCAACAACGACATCCTCGCGCTGACCAAACCGGAAGTCCCGGAATCGATTCACCGCGCCTATTTCGAG
GCCGGGGCCGACATTGCCGAAACCAATACCTTCAGCGCCAACCGCATCAGCCAGGCCGACTACGCCGCCGAACATCTCGT
GCGCGAGATCAACGTGGAAAGCGCCAGGCTCGCCCGCCGTGTCGCGGATGAGTTCACCGCCCGGGACGGCAAGCCGCGCT
TCGTCGCCGGCGCCATCGGCCCCACGAACAAGACCCTCTCGCTCAGCCCGGACGTCAACGATCCGGGATTCCGCGAGATC
GACTGGGATCACCTTGTCGATGTCTATGCCGAACAGGTCCACGCCCTTGTCGAAGGCGGCGCGGACTTCATCCTGATCGA
GACGGTCTTTGACACGCTCAACGCCAAGGCGGGTGTCATGTCGGTCCGTCAGGTCGAGAAGCAGCTCGGCCGTGAAATCC
CGATCATGATGTCGATGACGCTGACCGACCTTTCCGGCCGCAACCTCTCGGGGCACACGGTAGAGGCGTTCTGGTACGCG
ATCCGCCACGCAAGGCCCCTGACGGTCGGCCTCAACTGTTCGTTCGGTGCCACCCAGTTGCGCCCGCACGTCAAGGCGCT
TTCAGAAATCGCCGACACCCTGATCATGGTCTACCCCAACGCCGGCCTTCCCAACGAACTCGGCGAATATGACGAGATGC
CACAGACCACCGCCGGCCTCGTCAAGGAATGGGCGGATCACGGCCAGGTCAACGTCCTCGGCGGCTGCTGCGGTTCAACG
CCCGCGCACATCGCCGCCATGGCGCAGGCGGTGAAGGCGTTGCCTCCGCGCAAGATGGCTGTGCCGGAAACGGTAACGCG
GCTGGCAGGTCTCGAGCCGTTCATCATGGCTGCATAA

Upstream 100 bases:

>100_bases
GCGATTTCCACTTCTACACCCTCAACCGCGCCGAACTGTCCTATGCGATCTGCCACCTGCTTGGCCTTCGTCCCCGGACT
CCGGCGCTGGAGAAAGCCGC

Downstream 100 bases:

>100_bases
CCAAGTCCCCCTCCCGCAAGCGGGAGGGGTTAGGGGTGGGCAGCGGTCAAGCGCCCATCTCGAAGAAGAAAAGTCCACCC
CCGACCCCTCCCGCCTGCGG

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTLIPSPLIPSGARTAFLEQASQRILITDGAFGTEIQNFKLSEEDYAGTLGLAKDQKGNNDILALTKPEVPESIHRAYFE
AGADIAETNTFSANRISQADYAAEHLVREINVESARLARRVADEFTARDGKPRFVAGAIGPTNKTLSLSPDVNDPGFREI
DWDHLVDVYAEQVHALVEGGADFILIETVFDTLNAKAGVMSVRQVEKQLGREIPIMMSMTLTDLSGRNLSGHTVEAFWYA
IRHARPLTVGLNCSFGATQLRPHVKALSEIADTLIMVYPNAGLPNELGEYDEMPQTTAGLVKEWADHGQVNVLGGCCGST
PAHIAAMAQAVKALPPRKMAVPETVTRLAGLEPFIMAA

Sequences:

>Translated_358_residues
MTLIPSPLIPSGARTAFLEQASQRILITDGAFGTEIQNFKLSEEDYAGTLGLAKDQKGNNDILALTKPEVPESIHRAYFE
AGADIAETNTFSANRISQADYAAEHLVREINVESARLARRVADEFTARDGKPRFVAGAIGPTNKTLSLSPDVNDPGFREI
DWDHLVDVYAEQVHALVEGGADFILIETVFDTLNAKAGVMSVRQVEKQLGREIPIMMSMTLTDLSGRNLSGHTVEAFWYA
IRHARPLTVGLNCSFGATQLRPHVKALSEIADTLIMVYPNAGLPNELGEYDEMPQTTAGLVKEWADHGQVNVLGGCCGST
PAHIAAMAQAVKALPPRKMAVPETVTRLAGLEPFIMAA
>Mature_357_residues
TLIPSPLIPSGARTAFLEQASQRILITDGAFGTEIQNFKLSEEDYAGTLGLAKDQKGNNDILALTKPEVPESIHRAYFEA
GADIAETNTFSANRISQADYAAEHLVREINVESARLARRVADEFTARDGKPRFVAGAIGPTNKTLSLSPDVNDPGFREID
WDHLVDVYAEQVHALVEGGADFILIETVFDTLNAKAGVMSVRQVEKQLGREIPIMMSMTLTDLSGRNLSGHTVEAFWYAI
RHARPLTVGLNCSFGATQLRPHVKALSEIADTLIMVYPNAGLPNELGEYDEMPQTTAGLVKEWADHGQVNVLGGCCGSTP
AHIAAMAQAVKALPPRKMAVPETVTRLAGLEPFIMAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=339, Percent_Identity=48.6725663716814, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1790450, Length=338, Percent_Identity=56.508875739645, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535405, Length=347, Percent_Identity=48.4149855907781, Blast_Score=340, Evalue=6e-94,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38750; Mature: 38619

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIPSPLIPSGARTAFLEQASQRILITDGAFGTEIQNFKLSEEDYAGTLGLAKDQKGNN
CCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DILALTKPEVPESIHRAYFEAGADIAETNTFSANRISQADYAAEHLVREINVESARLARR
CEEEEECCCCHHHHHHHHHHCCCCHHHCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHH
VADEFTARDGKPRFVAGAIGPTNKTLSLSPDVNDPGFREIDWDHLVDVYAEQVHALVEGG
HHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ADFILIETVFDTLNAKAGVMSVRQVEKQLGREIPIMMSMTLTDLSGRNLSGHTVEAFWYA
CCEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
IRHARPLTVGLNCSFGATQLRPHVKALSEIADTLIMVYPNAGLPNELGEYDEMPQTTAGL
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCHHHHHH
VKEWADHGQVNVLGGCCGSTPAHIAAMAQAVKALPPRKMAVPETVTRLAGLEPFIMAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
TLIPSPLIPSGARTAFLEQASQRILITDGAFGTEIQNFKLSEEDYAGTLGLAKDQKGNN
CCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DILALTKPEVPESIHRAYFEAGADIAETNTFSANRISQADYAAEHLVREINVESARLARR
CEEEEECCCCHHHHHHHHHHCCCCHHHCCCCCCHHCCHHHHHHHHHHHHHCCHHHHHHHH
VADEFTARDGKPRFVAGAIGPTNKTLSLSPDVNDPGFREIDWDHLVDVYAEQVHALVEGG
HHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
ADFILIETVFDTLNAKAGVMSVRQVEKQLGREIPIMMSMTLTDLSGRNLSGHTVEAFWYA
CCEEEHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHH
IRHARPLTVGLNCSFGATQLRPHVKALSEIADTLIMVYPNAGLPNELGEYDEMPQTTAGL
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCHHHHHH
VKEWADHGQVNVLGGCCGSTPAHIAAMAQAVKALPPRKMAVPETVTRLAGLEPFIMAA
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]