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The map label for this gene is metH [H]

Identifier: 87201218

GI number: 87201218

Start: 3421708

End: 3424344

Strand: Reverse

Name: metH [H]

Synonym: Saro_3206

Alternate gene names: 87201218

Gene position: 3424344-3421708 (Counterclockwise)

Preceding gene: 87201219

Following gene: 87201215

Centisome position: 96.15

GC content: 64.39

Gene sequence:

>2637_bases
ATGACAAATACCCCCACCGGCTCGCGTTTCGTCAACGTCGGCGAACGCACCAACGTCACCGGCTCCGCCGCGTTCAAGAA
GCTGATCCTCGCGGGCGACTACGCCAAGGCGGTCGAAGTCGCGCGCCAGCAGGTCGAGAACGGCGCGCAGGTCATCGACG
TCAACATGGACGAGGGCCTGCTCGACGCGGTCGAGGCCATGACCACGTTCCTCAAGCTGATCGCCGCCGAGCCCGATATT
GCCCGCGTGCCGGTGATGATCGACAGCTCGAAGTGGGAAGTGATCGAAGCGGGCCTAAAGTGCGTTTCGGGCAAGCCCAT
CGTCAACTCGATCTCGATGAAGGAGGGCGAGGACGCCTTCCTCCACCACGCCCGCCTGTGCATGGACTATGGCGCTGCCG
TTGTCGTCATGGCGTTCGACGAGAAGGGCCAGGCCGATACGAAGGAGCGCAAGGTCGAGATCTGCGAGCGGGCCTACAAG
TTGCTCACCGGTATCGGCTTCCCGGCCGAAGACATCATCTTCGATCCCAACATCTTCGCCGTCGCCACCGGCATCGAGGA
GCATGACCGCTACGGGCTGGACTTCATCGAAGCCACGGCCGAGATCAAGGCGCGCTGTCCCCACGTCCATATCTCGGGCG
GCCTGTCGAACCTCTCGTTCTCGTTCCGCGGCAACGAGACAGTGCGCCGCGCGATGCATTCGGTGTTCCTCTACCACGCC
ATCCCTGCGGGCATGGACATGGCCATTGTCAACGCCGGCCAGCTCGACATCTACGACCAGATCGACCCGGTCCTCCGCGA
GGCTTGCGAGGACGTCGTGCTTAACCGCGCGCCTGCCGCGGGTGGTGACCAGACCGCGACCGAACGCCTGATCGAACTCG
CCGAAAGCTACAAGGGCAAGGACACCGTCGCCGAAAAGGCCGCCGAGGAATGGCGCGGCTGGGATGTCGTGCGCCGCCTG
GAACACGCGCTCGTCAAGGGCATCGATGCCCACGTCGTCGAAGACACCGAAGAGGCCCGCGCCGCCATCGCCGCGCGCGG
CGGTCGCCCGATCGAGGTGATCGAAGGCCCCTTGATGGAAGGCATGAACACGGTCGGGGACCTGTTCGGCTCGGGCAAGA
TGTTCCTGCCGCAGGTGGTCAAGTCCGCCCGCGTGATGAAGAAGGCCGTTGCCCACCTTATCCCCTATATCGAGGCGGAA
AAGGAAGAAGGCGCCAAGGCCAAGGGCCGGATCATAATGGCCACCGTCAAGGGCGACGTTCACGACATCGGCAAGAACAT
CGTCGGCGTGGTCCTGCAGTGCAACGGCTACGAAGTGATCGATCTGGGTGTCATGGTCCCCTGGGCGACGATCCTCGACA
CGGCGCAGAAGGAAGGCGTCGACATGATCGGCCTGTCCGGCCTCATCACGCCCTCGCTCGACGAGATGGTGACCGTTGCC
GAGGAAATGCAGCGCGCCGGGATGAGCATTCCGCTGCTGATCGGCGGCGCGACCACCAGCAAGGTCCACACCGCGCTGCG
CATCGATCCTGCCTATGACGGACCTGTCGTCCACGTGCTCGATGCCAGCCGCGCGGTCGGCGTCGCCTCGCAACTCCTTT
CGGATACGCAGGCGCAGGGCTTCATCAAGGCCACTGCCGCCGATTACCAGCATGTGCGCGATGCGCGCGCGGGCAAGGGC
GCATCAAAGCTGCTGAGCCTCGAAGACGCCCGGCTGAACGCCTTTTCGCCCGACTTTTCGGAAAAGGCTCCACCGCCCGA
ACAGCCGGGCCGCCACGTGTTCGAAGACTGGGACCTTACCGACCTTGTCGAGTGCTTCGACTGGACCCCGTTCTTCCGCT
CGTGGGAACTCGCCGGCACATGGCCGGCCATCCTCGACGATGAAATCGTCGGCGAATCCGCGCGCAATCTCTATGCGGAT
GCCCGCGCGATGCTCGACAGGATCATCAGCGAGAAATGGCTTACCGCCAAGGGCGTTGCCCAGTTCTGGCCCTGCGCGCG
CCATGGCGACGACGTCATCCTGCACCCGAACGACGACGAAACATCGGTGCGCCTGCCATTCCTGCGCCAGCAGTTCATCA
AGAGCCGTGGCCGCGCGAACTTCTGCCTTGCCGACTTCATCGACCCGGCGGGCGACTGGATCGGCGGCTTCGCGGTCGGC
ATCCACGGGATCGACGAACACCTCGCCCGCTTCAAGGCCGACAACGACGATTACTCGGACATCCTGCTGAAGGCCCTTGC
CGATCGCTTCGCCGAAGCCTTCGCCGAGCGCCTGCACCAGTACGTCCGCACCACCCTCTGGGCCTATGCTCCGGGCGAGC
AACTGACCAATGAGGCGCTGATCCGTGAGCAGTACCGCGGCATCCGTCCCGCGCCGGGCTATCCGGCTTGCCCTGACCAC
ACGCTCAAGCCGATCCTGTTCGACCTGCTCGACGCGACCAACACGGCCGGCATCACGCTGACCGAATCGCAGGCCATGCT
GCCCACCTCGGCGGTTTCGGGATTCTACTTCGCCCACCCCGAAAGCCAGTACTTCGGTGTCGCCACCATCGGTCGCGATC
AACTCGAGGACTACGCCGCCCGCCGTGGCTGGGATGTTTCCGCCATGGAACGCTGGCTTCGCCCCAACCTAGATTGA

Upstream 100 bases:

>100_bases
CAAGCGGGAGGGGTTAGGGGTGGGCAGCGGTCAAGCGCCCATCTCGAAGAAGAAAAGTCCACCCCCGACCCCTCCCGCCT
GCGGGAGGGGGGAGTAGAAG

Downstream 100 bases:

>100_bases
CGCGACAAAGCCCCCTCCGTTTCAACGGAGGGGGTAGCGGGCCGGCGCGCACTGCCCTGCGCCCGTCGGCTATTGCAGCC
GACTGGCCGCCACCCGCGAT

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 878; Mature: 877

Protein sequence:

>878_residues
MTNTPTGSRFVNVGERTNVTGSAAFKKLILAGDYAKAVEVARQQVENGAQVIDVNMDEGLLDAVEAMTTFLKLIAAEPDI
ARVPVMIDSSKWEVIEAGLKCVSGKPIVNSISMKEGEDAFLHHARLCMDYGAAVVVMAFDEKGQADTKERKVEICERAYK
LLTGIGFPAEDIIFDPNIFAVATGIEEHDRYGLDFIEATAEIKARCPHVHISGGLSNLSFSFRGNETVRRAMHSVFLYHA
IPAGMDMAIVNAGQLDIYDQIDPVLREACEDVVLNRAPAAGGDQTATERLIELAESYKGKDTVAEKAAEEWRGWDVVRRL
EHALVKGIDAHVVEDTEEARAAIAARGGRPIEVIEGPLMEGMNTVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPYIEAE
KEEGAKAKGRIIMATVKGDVHDIGKNIVGVVLQCNGYEVIDLGVMVPWATILDTAQKEGVDMIGLSGLITPSLDEMVTVA
EEMQRAGMSIPLLIGGATTSKVHTALRIDPAYDGPVVHVLDASRAVGVASQLLSDTQAQGFIKATAADYQHVRDARAGKG
ASKLLSLEDARLNAFSPDFSEKAPPPEQPGRHVFEDWDLTDLVECFDWTPFFRSWELAGTWPAILDDEIVGESARNLYAD
ARAMLDRIISEKWLTAKGVAQFWPCARHGDDVILHPNDDETSVRLPFLRQQFIKSRGRANFCLADFIDPAGDWIGGFAVG
IHGIDEHLARFKADNDDYSDILLKALADRFAEAFAERLHQYVRTTLWAYAPGEQLTNEALIREQYRGIRPAPGYPACPDH
TLKPILFDLLDATNTAGITLTESQAMLPTSAVSGFYFAHPESQYFGVATIGRDQLEDYAARRGWDVSAMERWLRPNLD

Sequences:

>Translated_878_residues
MTNTPTGSRFVNVGERTNVTGSAAFKKLILAGDYAKAVEVARQQVENGAQVIDVNMDEGLLDAVEAMTTFLKLIAAEPDI
ARVPVMIDSSKWEVIEAGLKCVSGKPIVNSISMKEGEDAFLHHARLCMDYGAAVVVMAFDEKGQADTKERKVEICERAYK
LLTGIGFPAEDIIFDPNIFAVATGIEEHDRYGLDFIEATAEIKARCPHVHISGGLSNLSFSFRGNETVRRAMHSVFLYHA
IPAGMDMAIVNAGQLDIYDQIDPVLREACEDVVLNRAPAAGGDQTATERLIELAESYKGKDTVAEKAAEEWRGWDVVRRL
EHALVKGIDAHVVEDTEEARAAIAARGGRPIEVIEGPLMEGMNTVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPYIEAE
KEEGAKAKGRIIMATVKGDVHDIGKNIVGVVLQCNGYEVIDLGVMVPWATILDTAQKEGVDMIGLSGLITPSLDEMVTVA
EEMQRAGMSIPLLIGGATTSKVHTALRIDPAYDGPVVHVLDASRAVGVASQLLSDTQAQGFIKATAADYQHVRDARAGKG
ASKLLSLEDARLNAFSPDFSEKAPPPEQPGRHVFEDWDLTDLVECFDWTPFFRSWELAGTWPAILDDEIVGESARNLYAD
ARAMLDRIISEKWLTAKGVAQFWPCARHGDDVILHPNDDETSVRLPFLRQQFIKSRGRANFCLADFIDPAGDWIGGFAVG
IHGIDEHLARFKADNDDYSDILLKALADRFAEAFAERLHQYVRTTLWAYAPGEQLTNEALIREQYRGIRPAPGYPACPDH
TLKPILFDLLDATNTAGITLTESQAMLPTSAVSGFYFAHPESQYFGVATIGRDQLEDYAARRGWDVSAMERWLRPNLD
>Mature_877_residues
TNTPTGSRFVNVGERTNVTGSAAFKKLILAGDYAKAVEVARQQVENGAQVIDVNMDEGLLDAVEAMTTFLKLIAAEPDIA
RVPVMIDSSKWEVIEAGLKCVSGKPIVNSISMKEGEDAFLHHARLCMDYGAAVVVMAFDEKGQADTKERKVEICERAYKL
LTGIGFPAEDIIFDPNIFAVATGIEEHDRYGLDFIEATAEIKARCPHVHISGGLSNLSFSFRGNETVRRAMHSVFLYHAI
PAGMDMAIVNAGQLDIYDQIDPVLREACEDVVLNRAPAAGGDQTATERLIELAESYKGKDTVAEKAAEEWRGWDVVRRLE
HALVKGIDAHVVEDTEEARAAIAARGGRPIEVIEGPLMEGMNTVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPYIEAEK
EEGAKAKGRIIMATVKGDVHDIGKNIVGVVLQCNGYEVIDLGVMVPWATILDTAQKEGVDMIGLSGLITPSLDEMVTVAE
EMQRAGMSIPLLIGGATTSKVHTALRIDPAYDGPVVHVLDASRAVGVASQLLSDTQAQGFIKATAADYQHVRDARAGKGA
SKLLSLEDARLNAFSPDFSEKAPPPEQPGRHVFEDWDLTDLVECFDWTPFFRSWELAGTWPAILDDEIVGESARNLYADA
RAMLDRIISEKWLTAKGVAQFWPCARHGDDVILHPNDDETSVRLPFLRQQFIKSRGRANFCLADFIDPAGDWIGGFAVGI
HGIDEHLARFKADNDDYSDILLKALADRFAEAFAERLHQYVRTTLWAYAPGEQLTNEALIREQYRGIRPAPGYPACPDHT
LKPILFDLLDATNTAGITLTESQAMLPTSAVSGFYFAHPESQYFGVATIGRDQLEDYAARRGWDVSAMERWLRPNLD

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=901, Percent_Identity=52.9411764705882, Blast_Score=895, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=888, Percent_Identity=60.0225225225225, Blast_Score=1024, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=898, Percent_Identity=51.0022271714922, Blast_Score=847, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 96252; Mature: 96121

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTPTGSRFVNVGERTNVTGSAAFKKLILAGDYAKAVEVARQQVENGAQVIDVNMDEGL
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCHH
LDAVEAMTTFLKLIAAEPDIARVPVMIDSSKWEVIEAGLKCVSGKPIVNSISMKEGEDAF
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LHHARLCMDYGAAVVVMAFDEKGQADTKERKVEICERAYKLLTGIGFPAEDIIFDPNIFA
HHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCEE
VATGIEEHDRYGLDFIEATAEIKARCPHVHISGGLSNLSFSFRGNETVRRAMHSVFLYHA
EECCCHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHH
IPAGMDMAIVNAGQLDIYDQIDPVLREACEDVVLNRAPAAGGDQTATERLIELAESYKGK
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
DTVAEKAAEEWRGWDVVRRLEHALVKGIDAHVVEDTEEARAAIAARGGRPIEVIEGPLME
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHCCCCCHHHHCCHHHH
GMNTVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPYIEAEKEEGAKAKGRIIMATVKGDV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
HDIGKNIVGVVLQCNGYEVIDLGVMVPWATILDTAQKEGVDMIGLSGLITPSLDEMVTVA
HHHCCHHEEEEEEECCEEEEEECHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
EEMQRAGMSIPLLIGGATTSKVHTALRIDPAYDGPVVHVLDASRAVGVASQLLSDTQAQG
HHHHHCCCCCEEEEECCCHHHHEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCC
FIKATAADYQHVRDARAGKGASKLLSLEDARLNAFSPDFSEKAPPPEQPGRHVFEDWDLT
EEEHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DLVECFDWTPFFRSWELAGTWPAILDDEIVGESARNLYADARAMLDRIISEKWLTAKGVA
HHHHHHCCCHHHHCCCCCCCCHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QFWPCARHGDDVILHPNDDETSVRLPFLRQQFIKSRGRANFCLADFIDPAGDWIGGFAVG
HHCCCCCCCCEEEECCCCCCCCEECHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHH
IHGIDEHLARFKADNDDYSDILLKALADRFAEAFAERLHQYVRTTLWAYAPGEQLTNEAL
HCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IREQYRGIRPAPGYPACPDHTLKPILFDLLDATNTAGITLTESQAMLPTSAVSGFYFAHP
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHCCEEEECC
ESQYFGVATIGRDQLEDYAARRGWDVSAMERWLRPNLD
CCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TNTPTGSRFVNVGERTNVTGSAAFKKLILAGDYAKAVEVARQQVENGAQVIDVNMDEGL
CCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCHH
LDAVEAMTTFLKLIAAEPDIARVPVMIDSSKWEVIEAGLKCVSGKPIVNSISMKEGEDAF
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LHHARLCMDYGAAVVVMAFDEKGQADTKERKVEICERAYKLLTGIGFPAEDIIFDPNIFA
HHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCEE
VATGIEEHDRYGLDFIEATAEIKARCPHVHISGGLSNLSFSFRGNETVRRAMHSVFLYHA
EECCCHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHH
IPAGMDMAIVNAGQLDIYDQIDPVLREACEDVVLNRAPAAGGDQTATERLIELAESYKGK
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
DTVAEKAAEEWRGWDVVRRLEHALVKGIDAHVVEDTEEARAAIAARGGRPIEVIEGPLME
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHEECCHHHHHHHHHHCCCCCHHHHCCHHHH
GMNTVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPYIEAEKEEGAKAKGRIIMATVKGDV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCH
HDIGKNIVGVVLQCNGYEVIDLGVMVPWATILDTAQKEGVDMIGLSGLITPSLDEMVTVA
HHHCCHHEEEEEEECCEEEEEECHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
EEMQRAGMSIPLLIGGATTSKVHTALRIDPAYDGPVVHVLDASRAVGVASQLLSDTQAQG
HHHHHCCCCCEEEEECCCHHHHEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHCC
FIKATAADYQHVRDARAGKGASKLLSLEDARLNAFSPDFSEKAPPPEQPGRHVFEDWDLT
EEEHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DLVECFDWTPFFRSWELAGTWPAILDDEIVGESARNLYADARAMLDRIISEKWLTAKGVA
HHHHHHCCCHHHHCCCCCCCCHHHHCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QFWPCARHGDDVILHPNDDETSVRLPFLRQQFIKSRGRANFCLADFIDPAGDWIGGFAVG
HHCCCCCCCCEEEECCCCCCCCEECHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHH
IHGIDEHLARFKADNDDYSDILLKALADRFAEAFAERLHQYVRTTLWAYAPGEQLTNEAL
HCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IREQYRGIRPAPGYPACPDHTLKPILFDLLDATNTAGITLTESQAMLPTSAVSGFYFAHP
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHCCEEEECC
ESQYFGVATIGRDQLEDYAARRGWDVSAMERWLRPNLD
CCCEEEEEECCHHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]